請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25563
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 廖秀娟(Vivian Hsiu-Chuan Liao) | |
dc.contributor.author | Yi-Chen Hsieh | en |
dc.contributor.author | 謝翼振 | zh_TW |
dc.date.accessioned | 2021-06-08T06:18:53Z | - |
dc.date.copyright | 2011-08-16 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-03 | |
dc.identifier.citation | Abernathy, C. O., Liu, Y. P., Longfellow, D., Aposhian, H. V., Beck, B., Fowler, B., Goyer, R., Menzer, R., Rossman, T., Thompson, C., and Waalkes, M. (1999). Arsenic: health effects, mechanisms of actions, and research issues. Environ Health Perspect 107, 593-597.
Alper, S. L., Darman, R. B., Chernova, M. N., and Dahl, N. K. (2002). The AE gene family of Cl/HCO3- exchangers. J Nephrol 15 Suppl 5, S41-53. Ames, B. N., Shigenaga, M. K., and Hagen, T. M. (1993). Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A 90, 7915-7922. Barbieri, M., Bonafe, M., Franceschi, C., and Paolisso, G. (2003). Insulin/IGF-I-signaling pathway: an evolutionarily conserved mechanism of longevity from yeast to humans. Am J Physiol Endocrinol Metab 285, E1064-1071. Beckman, K. B., and Ames, B. N. (1997). Oxidative decay of DNA. J Biol Chem 272, 19633-19636. Bevensee, M. O., Boron, W. F. (2008). Control of intracellular pH. Elisevir, New York. Boron, V. F., Hediger, M. A., Boulpaep, E. L., and Romero, M. F. (1997). The renal electrogenic Na+:HCO-3 cotransporter. J Exp Biol 200, 263-268. Cadenas, E. (1989). Biochemistry of oxygen toxicity. Annu Rev Biochem 58, 79-110. Chen, Y. C., Lin-Shiau, S. Y., and Lin, J. K. (1998a). Involvement of reactive oxygen species and caspase 3 activation in arsenite-induced apoptosis. J Cell Physiol 177, 324-333. Chen, Y. H., Wu, M. L., and Fu, W. M. (1998b). Regulation of acetylcholine release by intracellular acidification of developing motoneurons in Xenopus cell cultures. J Physiol 507 ( Pt 1), 41-53. Claeys, I., Simonet, G., Poels, J., Van Loy, T., Vercammen, L., De Loof, A., and Vanden Broeck, J. (2002). Insulin-related peptides and their conserved signal transduction pathway. Peptides 23, 807-816. Davison, K., Mann, K. K., Waxman, S., and Miller, W. H., Jr. (2004). JNK activation is a mediator of arsenic trioxide-induced apoptosis in acute promyelocytic leukemia cells. Blood 103, 3496-3502. Facchini, F. S., Hua, N., Abbasi, F., and Reaven, G. M. (2001). Insulin resistance as a predictor of age-related diseases. J Clin Endocrinol Metab 86, 3574-3578. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811. Fridovich, I. (1995). Superoxide radical and superoxide dismutases. Annu Rev Biochem 64, 97-112. Giulivi, C., Boveris, A., and Cadenas, E. (1995). Hydroxyl radical generation during mitochondrial electron transfer and the formation of 8-hydroxydesoxyguanosine in mitochondrial DNA. Arch Biochem Biophys 316, 909-916. Grichtchenko, II, Choi, I., Zhong, X., Bray-Ward, P., Russell, J. M., and Boron, W. F. (2001). Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger. J Biol Chem 276, 8358-8363. Gudz, T. I., Tserng, K. Y., and Hoppel, C. L. (1997). Direct inhibition of mitochondrial respiratory chain complex III by cell-permeable ceramide. J Biol Chem 272, 24154-24158. Henderson, S. T., and Johnson, T. E. (2001). daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans. Curr Biol 11, 1975-1980. Honda, Y., and Honda, S. (2002). Oxidative stress and life span determination in the nematode Caenorhabditis elegans. Ann N Y Acad Sci 959, 466-474. Inoue, M., Sato, E. F., Nishikawa, M., Hiramoto, K., Kashiwagi, A., and Utsumi, K. (2004). Free radical theory of apoptosis and metamorphosis. Redox Rep 9, 237-247. Itoh, T., Zhang, Y. F., Murai, S., Saito, H., Nagahama, H., Miyate, H., Saito, Y., and Abe, E. (1990). The effect of arsenic trioxide on brain monoamine metabolism and locomotor activity of mice. Toxicol Lett 54, 345-353. Iwama, K., Nakajo, S., Aiuchi, T., and Nakaya, K. (2001). Apoptosis induced by arsenic trioxide in leukemia U937 cells is dependent on activation of p38, inactivation of ERK and the Ca2+-dependent production of superoxide. Int J Cancer 92, 518-526. Jing, Y., Dai, J., Chalmers-Redman, R. M., Tatton, W. G., and Waxman, S. (1999). Arsenic trioxide selectively induces acute promyelocytic leukemia cell apoptosis via a hydrogen peroxide-dependent pathway. Blood 94, 2102-2111. Kamath, R. S., Martinez-Campos, M., Zipperlen, P., Fraser, A. G., and Ahringer, J. (2001). Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol 2, RESEARCH0002. Kannan, G. M., Tripathi, N., Dube, S. N., Gupta, M., and Flora, S. J. (2001). Toxic effects of arsenic (III) on some hematopoietic and central nervous system variables in rats and guinea pigs. J Toxicol Clin Toxicol 39, 675-682. Kao, G., Nordenson, C., Still, M., Ronnlund, A., Tuck, S., and Naredi, P. (2007). ASNA-1 positively regulates insulin secretion in C. elegans and mammalian cells. Cell 128, 577-587. Kimura, K. D., Tissenbaum, H. A., Liu, Y., and Ruvkun, G. (1997). daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. Science 277, 942-946. Kobayashi, H., Yuyama, A., Ishihara, M., and Matsusaka, N. (1987). Effects of arsenic on cholinergic parameters in brain in vitro. Neuropharmacology 26, 1707-1713. Lee, S. S., Kennedy, S., Tolonen, A. C., and Ruvkun, G. (2003). DAF-16 target genes that control C. elegans life-span and metabolism. Science 300, 644-647. Lee, T. C., Ko, J. L., and Jan, K. Y. (1989). Differential cytotoxicity of sodium arsenite in human fibroblasts and Chinese hamster ovary cells. Toxicology 56, 289-299. Lerman, S. A., Clarkson, T. W., and Gerson, R. J. (1983). Arsenic uptake and metabolism by liver cells is dependent on arsenic oxidation state. Chem Biol Interact 45, 401-406. Lin, K., Hsin, H., Libina, N., and Kenyon, C. (2001). Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling. Nat Genet 28, 139-145. Loh, K. P., Huang, S. H., De Silva, R., Tan, B. K., and Zhu, Y. Z. (2006). Oxidative stress: apoptosis in neuronal injury. Curr Alzheimer Res 3, 327-337. Mahieux, R., Pise-Masison, C., Gessain, A., Brady, J. N., Olivier, R., Perret, E., Misteli, T., and Nicot, C. (2001). Arsenic trioxide induces apoptosis in human T-cell leukemia virus type 1- and type 2-infected cells by a caspase-3-dependent mechanism involving Bcl-2 cleavage. Blood 98, 3762-3769. Mahoney, T. R., Luo, S., and Nonet, M. L. (2006). Analysis of synaptic transmission in Caenorhabditis elegans using an aldicarb-sensitivity assay. Nat Protoc 1, 1772-1777. McElwee, J., Bubb, K., and Thomas, J. H. (2003). Transcriptional outputs of the Caenorhabditis elegans forkhead protein DAF-16. Aging Cell 2, 111-121. Mello, C., and Fire, A. (1995). DNA transformation. Methods Cell Biol 48, 451-482. Mello, C. C., Kramer, J. M., Stinchcomb, D., and Ambros, V. (1991). Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J 10, 3959-3970. Morris, J. Z., Tissenbaum, H. A., and Ruvkun, G. (1996). A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans. Nature 382, 536-539. Murphy, C. T., McCarroll, S. A., Bargmann, C. I., Fraser, A., Kamath, R. S., Ahringer, J., Li, H., and Kenyon, C. (2003). Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature 424, 277-283. Nagaraja, T. N., and Desiraju, T. (1993). Regional alterations in the levels of brain biogenic amines, glutamate, GABA, and GAD activity due to chronic consumption of inorganic arsenic in developing and adult rats. Bull Environ Contam Toxicol 50, 100-107. Ogg, S., Paradis, S., Gottlieb, S., Patterson, G. I., Lee, L., Tissenbaum, H. A., and Ruvkun, G. (1997). The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature 389, 994-999. Oh, S. W., Mukhopadhyay, A., Dixit, B. L., Raha, T., Green, M. R., and Tissenbaum, H. A. (2006). Identification of direct DAF-16 targets controlling longevity, metabolism and diapause by chromatin immunoprecipitation. Nat Genet 38, 251-257. Palmer, H. J., and Paulson, K. E. (1997). Reactive oxygen species and antioxidants in signal transduction and gene expression. Nutr Rev 55, 353-361. Parker, A. B., and Gilbert, D. G. (2008). Brain activity during anticipation of smoking-related and emotionally positive pictures in smokers and nonsmokers: a new measure of cue reactivity. Nicotine Tob Res 10, 1627-1631. Parker, M. D., Musa-Aziz, R., Rojas, J. D., Choi, I., Daly, C. M., and Boron, W. F. (2008). Characterization of human SLC4A10 as an electroneutral Na/HCO3 cotransporter (NBCn2) with Cl- self-exchange activity. J Biol Chem 283, 12777-12788. Romero, M. F., Fulton, C. M., and Boron, W. F. (2004). The SLC4 family of HCO3 - transporters. Pflugers Arch 447, 495-509. Romero, M. F., Henry, D., Nelson, S., Harte, P. J., Dillon, A. K., and Sciortino, C. M. (2000). Cloning and characterization of a Na+-driven anion exchanger (NDAE1). A new bicarbonate transporter. J Biol Chem 275, 24552-24559. Roos, A., and Boron, W. F. (1981). Intracellular pH. Physiol Rev 61, 296-434. Roth, G. S., Lane, M. A., Ingram, D. K., Mattison, J. A., Elahi, D., Tobin, J. D., Muller, D., and Metter, E. J. (2002). Biomarkers of caloric restriction may predict longevity in humans. Science 297, 811. Roy, A., Manna, P., and Sil, P. C. (2009). Prophylactic role of taurine on arsenic mediated oxidative renal dysfunction via MAPKs/ NF-kappaB and mitochondria dependent pathways. Free Radic Res 43, 995-1007. Russell, J. M., and Boron, W. F. (1976). Role of choloride transport in regulation of intracellular pH. Nature 264, 73-74. Samuel, S., Kathirvel, R., Jayavelu, T., and Chinnakkannu, P. (2005). Protein oxidative damage in arsenic induced rat brain: influence of DL-alpha-lipoic acid. Toxicol Lett 155, 27-34. Schreck, R., and Baeuerle, P. A. (1991). A role for oxygen radicals as second messengers. Trends Cell Biol 1, 39-42. Schwiening, C. J., and Boron, W. F. (1994). Regulation of intracellular pH in pyramidal neurones from the rat hippocampus by Na(+)-dependent Cl(-)-HCO3- exchange. J Physiol 475, 59-67. Sherman, T., Chernova, M. N., Clark, J. S., Jiang, L., Alper, S. L., and Nehrke, K. (2005). The abts and sulp families of anion transporters from Caenorhabditis elegans. Am J Physiol Cell Physiol 289, C341-351. Shi, H., Shi, X., and Liu, K. J. (2004). Oxidative mechanism of arsenic toxicity and carcinogenesis. Mol Cell Biochem 255, 67-78. Shila, S., Kokilavani, V., Subathra, M., and Panneerselvam, C. (2005a). Brain regional responses in antioxidant system to alpha-lipoic acid in arsenic intoxicated rat. Toxicology 210, 25-36. Shila, S., Subathra, M., Devi, M. A., and Panneerselvam, C. (2005b). Arsenic intoxication-induced reduction of glutathione level and of the activity of related enzymes in rat brain regions: reversal by DL-alpha-lipoic acid. Arch Toxicol 79, 140-146. Stadtman, E. R. (1992). Protein oxidation and aging. Science 257, 1220-1224. Studier, F. W., and Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-130. Timmons, L., Court, D. L., and Fire, A. (2001). Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263, 103-112. Timmons, L., and Fire, A. (1998). Specific interference by ingested dsRNA. Nature 395, 854. Tripathi, N., Kannan, G. M., Pant, B. P., Jaiswal, D. K., Malhotra, P. R., and Flora, S. J. (1997). Arsenic-induced changes in certain neurotransmitter levels and their recoveries following chelation in rat whole brain. Toxicol Lett 92, 201-208. Tseng, C. H., Chong, C. K., Chen, C. J., and Tai, T. Y. (1996). Dose-response relationship between peripheral vascular disease and ingested inorganic arsenic among residents in blackfoot disease endemic villages in Taiwan. Atherosclerosis 120, 125-133. Tseng, Y. Y., Yu, C. W., and Liao, V. H. (2007). Caenorhabditis elegans expresses a functional ArsA. FEBS J 274, 2566-2572. Vahidnia, A., van der Voet, G. B., and de Wolff, F. A. (2007). Arsenic neurotoxicity-a review. Hum Exp Toxicol 26, 823-832. Wang, C. Z., Yano, H., Nagashima, K., and Seino, S. (2000). The Na+-driven Cl-/HCO3- exchanger. Cloning, tissue distribution, and functional characterization. J Biol Chem 275, 35486-35490. Wang, Z., Conforti, L., Petrovic, S., Amlal, H., Burnham, C. E., and Soleimani, M. (2001). Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning, expression, and renal distribution. Kidney Int 59, 1405-1414. Yadav, R. S., Sankhwar, M. L., Shukla, R. K., Chandra, R., Pant, A. B., Islam, F., and Khanna, V. K. (2009). Attenuation of arsenic neurotoxicity by curcumin in rats. Toxicol Appl Pharmacol 240, 367-376. Yamanaka, K., and Okada, S. (1994). Induction of lung-specific DNA damage by metabolically methylated arsenics via the production of free radicals. Environ Health Perspect 102 Suppl 3, 37-40. Zheng, Y., Shi, Y., Tian, C., Jiang, C., Jin, H., Chen, J., Almasan, A., Tang, H., and Chen, Q. (2004). Essential role of the voltage-dependent anion channel (VDAC) in mitochondrial permeability transition pore opening and cytochrome c release induced by arsenic trioxide. Oncogene 23, 1239-1247. Zhong, W., and Sternberg, P. W. (2006). Genome-wide prediction of C. elegans genetic interactions. Science 311, 1481-1484. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25563 | - |
dc.description.abstract | 砷是一種自然存在的毒性物質,其毒性危性危害了世界上許多的人,而在文獻中指出砷毒性可能會影響神經系統的病變,但是關於砷對神經系統的影響卻沒有太多的資料,而在目前的研究中,砷對神經系統的影響已經在線蟲中被研究。砷也是一種可以誘導產生活性氧分子的化學物質,我們在線蟲中發現一種依賴鈉離子來傳輸氯離子跟碳酸氫根離子的傳輸蛋白:ABTS-1,這個傳輸蛋白可以保護線蟲去抵抗砷所造成的毒性。此外,基因轉殖的線蟲abts-1::GFP也顯示出abts-1主要表達在蟲體的神經元以及皮下組織,但是在經過砷的暴露之後,在咽喉以及體壁肌肉細胞有更加明顯的表達;相反的,在短暫的暴露juglone誘導所產生的氧化壓力後,咽喉及體壁肌肉細胞的表達卻有明顯的下降,而基因轉殖線蟲在暴露砷之後,與未暴露的線蟲比較起來,其mRNA的表達也有顯著的上升。此外缺少abts-1的線蟲對於神經干擾物質aldicarb及levamisole造成的癱瘓也相當的敏感,若是環境中有砷的影響之下,癱瘓的速度會更加的明顯。另外,對於abts-1突變或者是基因表達被抑制的線蟲,比起正常的線蟲有著更長的生命;同樣的,我們發現在缺乏abts-1的線蟲對於氧化壓力有著較高的抵抗力,而這兩個影響可能都與胰島素信號通路的調控有關聯,砷造成的毒性可能藉由其他機制的傷害而非砷誘導的氧化壓力。最後,我們指出砷、胰島素信號通路以及碳酸氫鹽傳輸蛋白ABTS-1可能存在著相互影響的關係。 | zh_TW |
dc.description.abstract | Arsenic poisoning affects millions of people worldwide. Although there is evidence to suggest that the nervous system is a target of arsenic, relatively little information is known regarding its effects on nervous system. The effects of arsenite on the nervous system in Caenorhabditis elegans were investigated in the present study. Arsenic is also a chemical which can induce the generation of reactive oxygen species. We found that abts-1, which encodes a Na+-dependent Cl-/HCO3- transporter, is required to protect C. elegans from arsenite toxicity. The transgenic strain abts-1::GFP showed the expression of abts-1 is primarily on the neurons and the hypodernis, but stronger expression was also found in the pharynx and body wall muscle cells after exposure to arsenite. On the contrary, the level of GFP decreased after exposing to juglone. The mRNA expression of abts-1 in transgenic strain increased after arsenite exposure comparing to those unexposed animals. We showed that worms lacking of abts-1 is hypersensitive to the paralytic effects of the cholinesterase inhibitor, aldicarb, and the nicotinic acetylcholine receptor agonist, levamisole. Arsenic exposure can enhance the effect to aldicarb and levamisole in abts-1 mutant worms. Moreover, we showed that abts-1 mutants and RNAi-mediated abts-1 knockdown worms have an increase in life span comparing to wild-type worms, and the regulation between abts-1 and insulin signaling. We also showed that lacking of abts-1 can resist to juglone-induced oxidative stress, which might be regulated by insulin signaling pathway. It means that the function of abts-1 to protect C. elegans from arsenite toxicity is not by decreasing the arsenite-induced oxidative stress. Our results indicate the effects of arsenite, insulin/IGF-1 signaling, and the ABTS-1 bicarbonate transporter. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T06:18:53Z (GMT). No. of bitstreams: 1 ntu-100-R98622032-1.pdf: 979483 bytes, checksum: 6a238b30c2b33bedcbe0b2c435ec3568 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 致謝..................................................................................I
中文摘要........................................................................III ABSTRACT...................................................................IV TABLE OF CONTENTS..............................................VI LIST OF TABLES.....................................................VIII LIST OF FIGURES......................................................IX Abbreviations................................................................XI CHAPTER 1 INTRODUCTION...................................1 1.1 Arsenic.................................................................................................1 1.2 Oxidative stress...................................................................................4 1.3 Bicarbonate transporter.....................................................................6 1.4 Insulin/insulin growth factor 1 signaling pathway..........................7 1.5 Caenorhabditis elegans......................................................................10 1.6 Purpose of study................................................................................12 CHAPTER 2 MATERIALS AND METHODS...........14 2.1 Chemicals...........................................................................................14 2.2 Strain, clone, and culture conditions...............................................14 2.3 RNA interference...............................................................................15 2.4 Paralysis assay...................................................................................16 2.5 Life span assay...................................................................................16 2.6 Expression of transgenic C. elegans.................................................17 2.7 Oxidative stress..................................................................................17 2.8 Statistical analysis..............................................................................18 CHAPTER 3 RESULTS.................................................19 3.1 Prediction of abts-1 interacted gene..................................................19 3.2 ABTS-1 is required for As(III) tolerance in C. elegans...................19 3.3 Effects of As(III) on abts-1 transcription in transgenic C. elegans.................................................................................................20 3.4 Effect of As(III) and abts-1 in cholinergic signaling........................25 3.5 Life span assay and the regulation in insulin signaling pathway...32 3.6 Antioxidant assay and the regulation in insulin signaling pathway...............................................................................................36 3.7 Effect of oxidative stress on abts-1 transgenic in transgenic C. elegans................................................................................................41 CHAPTER 4 DISCUSSION..........................................47 CHAPTER 5 CONCLUSION.......................................52 REFERENCE.................................................................54 | |
dc.language.iso | en | |
dc.title | Caenorhabditis elegans碳酸氫鹽傳輸蛋白ABTS-1之功能探討 | zh_TW |
dc.title | Characterization of the bicarbonate transporter ABTS-1 in Caenorhabditis elegans | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳佩貞(Pei-Jen Chen),劉逸軒(I-Hsuan Liu) | |
dc.subject.keyword | 砷線蟲,ABTS-1,RNA干擾,氧化壓力,胰島素信號通路,碳酸氫鹽傳輸蛋白, | zh_TW |
dc.subject.keyword | Arsenic,Caenorhabditis elegans,ABTS-1,RNAi,oxidative stress,insulin/IGF-1 signaling pathway,bicarbonate transporter, | en |
dc.relation.page | 61 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-08-03 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物環境系統工程學研究所 | zh_TW |
顯示於系所單位: | 生物環境系統工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 956.53 kB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。