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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
dc.contributor.author | Wei-Jhih Cheng | en |
dc.contributor.author | 程偉智 | zh_TW |
dc.date.accessioned | 2021-06-08T06:02:37Z | - |
dc.date.copyright | 2007-07-31 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-25 | |
dc.identifier.citation | Cho,M.I., Matsuda,N., Lin,W.L., Moshier,A., and Ramakrishnan,P.R. (1992). In vitro formation of mineralized nodules by periodontal ligament cells from the rat. Calcif. Tissue Int. 50, 459-467.
Frau,S., Bernadou,J., and Meunier,B. (1997). Nuclease activity and binding characteristics of a cationic 'manganese porphyrin-bis(benzimidazole) dye (Hoechst 33258)' conjugate. Bioconjug. Chem. 8, 222-231. Grueterich,M., Espana,E., and Tseng,S.C. (2002). Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane. Invest Ophthalmol. Vis. Sci. 43, 63-71. Ivanovski,S., Haase,H.R., and Bartold,P.M. (2001). Expression of bone matrix protein mRNAs by primary and cloned cultures of the regenerative phenotype of human periodontal fibroblasts. J. Dent. Res. 80, 1665-1671. Jansen,J.A., den Braber,E.T., Walboomers,X.F., and de Ruijter,J.E. (1999). Soft tissue and epithelial models. Adv. Dent. Res. 13, 57-66. Kamalia,N., McCulloch,C.A., Tenebaum,H.C., and Limeback,H. (1992). Dexamethasone recruitment of self-renewing osteoprogenitor cells in chick bone marrow stromal cell cultures. Blood 79, 320-326. Kurpakus,M.A., Daneshvar,C., Davenport,J., and Kim,A. (1999). Human corneal epithelial cell adhesion to laminins. Curr. Eye Res. 19, 106-114. Lian,J.B., and Stein,G.S. (1995). Development of the osteoblast phenotype: molecular mechanisms mediating osteoblast growth and differentiation. Iowa Orthop. J. 15, 118-140. Lynch,M.P., Stein,J.L., Stein,G.S., and Lian,J.B. (1995). The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. Exp. Cell Res. 216, 35-45. Marquez-San,M.S., Rupf,S., Frenzel,J., and Eschrich,K. (2003). The effects of extracts from periodontopathic bacteria on human periodontal fibroblasts stimulated with mineralization supplements. J. Oral Sci. 45, 127-137. Nohutcu,R.M., McCauley,L.K., Shigeyama,Y., and Somerman,M.J. (1996). Expression of mineral-associated proteins by periodontal ligament cells: in vitro vs. ex vivo. J. Periodontal Res. 31, 369-372. Owen,T.A., Aronow,M., Shalhoub,V., Barone,L.M., Wilming,L., Tassinari,M.S., Kennedy,M.B., Pockwinse,S., Lian,J.B., and Stein,G.S. (1990). Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J. Cell Physiol 143, 420-430. Oyen,M.L., Calvin,S.E., and Cook,R.F. (2004). Uniaxial stress-relaxation and stress-strain responses of human amnion. J. Mater. Sci. Mater. Med. 15, 619-624. Palaiologou,A.A., Yukna,R.A., Moses,R., and Lallier,T.E. (2001). Gingival, dermal, and periodontal ligament fibroblasts express different extracellular matrix receptors. J. Periodontol. 72, 798-807. Parry,S., and Strauss,J.F., III (1998). Premature rupture of the fetal membranes. N. Engl. J. Med. 338, 663-670. Rinastiti,M., Harijadi, Santoso,A.L., and Sosroseno,W. (2006). Histological evaluation of rabbit gingival wound healing transplanted with human amniotic membrane. Int. J. Oral Maxillofac. Surg. 35, 247-251. Saito,Y., Yoshizawa,T., Takizawa,F., Ikegame,M., Ishibashi,O., Okuda,K., Hara,K., Ishibashi,K., Obinata,M., and Kawashima,H. (2002). A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2. J. Cell Sci. 115, 4191-4200. Shiga,M., Kapila,Y.L., Zhang,Q., Hayami,T., and Kapila,S. (2003). Ascorbic acid induces collagenase-1 in human periodontal ligament cells but not in MC3T3-E1 osteoblast-like cells: potential association between collagenase expression and changes in alkaline phosphatase phenotype. J. Bone Miner. 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Nuclease activity and binding characteristics of a cationic 'manganese porphyrin-bis(benzimidazole) dye (Hoechst 33258)' conjugate. Bioconjug. Chem. 8, 222-231. Grueterich,M., Espana,E., and Tseng,S.C. (2002). Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane. Invest Ophthalmol. Vis. Sci. 43, 63-71. Grueterich,M., and Tseng,S.C. (2002). Human limbal progenitor cells expanded on intact amniotic membrane ex vivo. Arch. Ophthalmol. 120, 783-790. Hamilton,V., Yuan,Y., Rigney,D.A., Puckett,A.D., Ong,J.L., Yang,Y., Elder,S.H., and Bumgardner,J.D. (2006). Characterization of chitosan films and effects on fibroblast cell attachment and proliferation. J. Mater. Sci. Mater. Med. 17, 1373-1381. Harris,R.J. (1998). Root coverage with a connective tissue with partial thickness double pedicle graft and an acellular dermal matrix graft: a clinical and histological evaluation of a case report. J. Periodontol. 69, 1305-1311. 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Staining properties of deepithelialized human amniotic membrane. Cornea 24, 853-856. Kim,J.S., Kim,J.C., Na,B.K., Jeong,J.M., and Song,C.Y. (2000). Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn. Exp. Eye Res. 70, 329-337. Kurpakus,M.A., Daneshvar,C., Davenport,J., and Kim,A. (1999). Human corneal epithelial cell adhesion to laminins. Curr. Eye Res. 19, 106-114. Lallier,T.E., Spencer,A., and Fowler,M.M. (2005). Transcript profiling of periodontal fibroblasts and osteoblasts. J. Periodontol. 76, 1044-1055. Le Du,M.H., and Millan,J.L. (2002). Structural evidence of functional divergence in human alkaline phosphatases. J. Biol. Chem. 277, 49808-49814. Lekic,P., Sodek,J., and McCulloch,C.A. (1996). Osteopontin and bone sialoprotein expression in regenerating rat periodontal ligament and alveolar bone. Anat. Rec. 244, 50-58. Lian,J.B., and Stein,G.S. (1995). 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Marquez-San,M.S., Rupf,S., Frenzel,J., and Eschrich,K. (2003). The effects of extracts from periodontopathic bacteria on human periodontal fibroblasts stimulated with mineralization supplements. J. Oral Sci. 45, 127-137. Meller,D., and Tseng,S.C. (1999). Conjunctival epithelial cell differentiation on amniotic membrane. Invest Ophthalmol. Vis. Sci. 40, 878-886. Nohutcu,R.M., McCauley,L.K., Koh,A.J., and Somerman,M.J. (1997). Expression of extracellular matrix proteins in human periodontal ligament cells during mineralization in vitro. J. Periodontol. 68, 320-327. Nohutcu,R.M., McCauley,L.K., Shigeyama,Y., and Somerman,M.J. (1996). Expression of mineral-associated proteins by periodontal ligament cells: in vitro vs. ex vivo. J. Periodontal Res. 31, 369-372. Owen,T.A., Aronow,M., Shalhoub,V., Barone,L.M., Wilming,L., Tassinari,M.S., Kennedy,M.B., Pockwinse,S., Lian,J.B., and Stein,G.S. (1990). 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25112 | - |
dc.description.abstract | 人類羊膜是一天然生物性材料,目前已有基礎及臨床研究報告顯示羊膜可被成功應用於一些醫療用途,例如作為皮膚燒燙傷之傷口貼附材料、眼角膜的重建等,至於利用羊膜做為口腔內組織再生的相關研究,迄今仍為數極少。本研究之目的是探討將人類羊膜作為細胞貼附基質時對於牙周細胞生長的影響,以評估羊膜應用於口腔內組織修復的可行性。
本研究第一部份是將兩種口腔細胞:牙周韌帶纖維細胞與牙齦纖維細胞分別在體外培養於羊膜基質層與經去細胞處理的基底膜層,並對其活性、形態與成骨分化潛力表現進行評估。第二部份則嘗試將幾丁聚醣與羊膜形成一複合材料,對其強度進行測試,並且進行動物實驗,將羊膜與此複合材料分別應用於口腔內頰側黏膜之傷口修復。 本實驗之結果發現: 相較於培養於組織培養皿上的生長,上述兩種口腔細胞培養在羊膜上時,都具有相當甚至更優的活性,並呈現類似纖維細胞的形態,此外,藉由聚合酶連鎖反應(RT-PCR)與鹼性磷酸酶(Alkaline phosphatase,ALP)活性測定發現,牙周韌帶纖維細胞在羊膜上仍具有顯著的ALP表現性,由於牙周韌帶細胞是一具有骨母細胞分化特性的纖維細胞,上述結果顯示羊膜能使牙周韌帶細胞保有其特殊功能性。 為加強羊膜此一天然生物材料的機械強度,本研究藉由覆蓋的方式使幾丁聚醣高分子溶液在經去細胞處理的羊膜基底膜上形成一層薄膜,藉由此方式形成的複合材料經單軸向拉伸測試發現其所能承載的拉力大小明顯上升。此外,本研究將羊膜與其複合材料貼附於米格魯犬頰側黏膜的傷口上,以評估其作為傷口敷材的效果,結果顯示: 無論是以羊膜或幾丁聚醣與羊膜複合材料覆蓋傷口,傷口在三週後都有完整且良好的癒合效果,尤其是羊膜複合材料覆蓋之頰側黏膜,傷口收縮程度最小,在組織學鏡檢下,可見表皮細胞遷移至傷口處,並生長形成多層的扁平麟狀表皮細胞,下方則充滿新生的膠原纖維組織。 | zh_TW |
dc.description.abstract | Human amniotic membrane is a nature biomaterial which has been used in several medical treatments such as cornea reconstruction and as a biological dressing for skin wounds and burns. The purpose of this study was to investigate the possibility of applying human amnions for oral tissue repair by evaluating the growth and behavior of periodontal ligament fibroblast and gingival fibroblasts cultured on the denuded human amniotic membrane.
Periodontal ligament fibroblasts on the amnion stroma and gingival fibroblasts were cultured on the basement membrane of denuded amnion. Cell viabilities, morphologies and functions were evaluated. Moreover, we tried to combine the chitosan and the amnion to become a composite material. Then its mechanical properties and the performance being as a dressing material in the surgical defects of the Beagle dog’s oral mucosa were evaluated. The results showed that gingival and periodontal ligament cells could grow very well when cultured on the basement membrane side and stromal side of denuded amnion, respectively. Furthermore, the periondontal ligament fibroblasts expressed significant ly elevated ALP activity when cultured on the stroma. To increase the strength of the amnion, chitosan was coated onto the denuded amnion’s basement membrane. The result of tensile test reveals that this composite material indeed became stronger. In the animal experiment, dressing of amniotic membrane might facilitate the epithelialization of wound surface and neo-collagen formation underneath the wound surface. Our results suggest the potential of amniotic membrane being as the matrix scaffold for periodontal cell growth and that amniotic membrane transplantation might promote the wound healing of oral mucosa. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T06:02:37Z (GMT). No. of bitstreams: 1 ntu-96-R94548050-1.pdf: 2477027 bytes, checksum: 0ff63001a696cd0f3e5408466b27b949 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 致謝…………………………………………………………………………………I
表次目錄……………………………………………………………………………IV 圖次目錄……………………………………………………………………………V 中文摘要……………………………………………………………………………VII 英文摘要……………………………………………………………………………IX 第一章 引言…………………………………………………………………………1 一 牙周組織及其缺損…………………………………………………………1 二 牙周韌帶細胞之特性………………………………………………………3 三 牙齦纖維細胞之特性………………………………………………………4 四 細胞成骨指標基因之表現…………………………………………………4 五 人類羊膜的組織型態與組成………………………………………………5 六 幾丁聚醣薄膜之性質………………………………………………………7 七 細胞於羊膜與幾丁聚醣薄膜之體外培養實驗模式…………………………8 八 羊膜的機械性質……………………………………………………………9 九 羊膜運用於傷口癒合之模式…………………………………………………9 第二章 實驗目的……………………………………………………………………11 第三章 實驗材料與方法……………………………………………………………12 一 細胞培養…………………………………………………………………12 二 去細胞羊膜之製備…………………………………………………………12 三 幾丁聚醣薄膜之製備………………………………………………………13 四 去細胞羊膜複合幾丁聚醣材料之製備……………………………………13 五 Hoechst 33258 染色………………………………………………………14 六 組織切片及蘇木紫與伊紅(Hematoxylin & Eosin)染色……………………14 七 掃瞄式電子顯微鏡(SEM, Scanning Electronic Microscope)標本製備……15 八 MTT(3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide))測試………16 九 鹼性磷酸酶(Alkaline Phosphatase)活性…………………………………17 十 機械強度測試………………………………………………………………18 十一 促進基質礦化實驗之成骨基因表現……………………………………19 十二 動物試驗…………………………………………………………………22 第四章 結果…………………………………………………………………………24 一 MTT測試……………………………………………………………………24 二 掃描式電子顯微鏡(SEM)觀察………………………………………………24 三Hoechst 33258結果…………………………………………………………25 四 組織切片…………………………………………………………………26 五 成骨基因表現………………………………………………………………26 六 ALP蛋白質活性定量………………………………………………………27 七 機械強度測試………………………………………………………………28 八 頰側黏膜傷口覆蓋之動物實驗………………………………………………28 第五章 討論…………………………………………………………………………30 一 去細胞處理之羊膜基底膜層與羊膜基質層…………………………………30 二 牙周韌帶細胞及牙齦纖維細胞培養於羊膜時的型態與活性………………30 三 促牙周韌帶細胞基質礦化之研究…………………………………………33 四 羊膜複合材料………………………………………………………………35 五 動物實驗…………………………………………………………………37 第六章 結論…………………………………………………………………………39 第七章 未來研究方向………………………………………………………………40 參考文獻……………………………………………………………………………65 | |
dc.language.iso | zh-TW | |
dc.title | 人類牙周細胞培養於羊膜基質之研究 | zh_TW |
dc.title | Study of human periodontal cells cultured on amniotic membrane matrix | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳羿貞,李建南,林宏殷,李玫樺 | |
dc.subject.keyword | 人類羊膜,牙周韌帶纖維細胞,牙齦纖維細胞,礦化誘導,口腔黏膜, | zh_TW |
dc.subject.keyword | human amniotic membrane,periodontal ligament fibroblasts,gingival fibroblasts,mineralization,oral mucosa, | en |
dc.relation.page | 73 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2007-07-26 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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