請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24620完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 錢宗良(Chung-Liang Chien) | |
| dc.contributor.author | Fong-Di Wei | en |
| dc.contributor.author | 魏凨笛 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:33:36Z | - |
| dc.date.copyright | 2011-10-07 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-27 | |
| dc.identifier.citation | References:
Bellairs R, Osmond M (2005) The atlas of chick development. Chapter 9, Unit 86 87. Bennett GS (1987) Changes in intermediate filament composition during neurogenesis. Curr Top Dev Biol 21:151-183. Bennett GS, Hollander BA, Laskowska D (1988) Expression and phosphorylation of the mid-sized neurofilament protein NF-M during chick spinal cord neurogenesis. J Neurosci Res 21:376-390. Carden MJ, Trojanowski JQ, Schlaepfer WW, Lee VM (1987) Two-stage expression of neurofilament polypeptides during rat neurogenesis with early establishment of adult phosphorylation patterns. J Neurosci 7:3489-3504. Chiappe LM (1995) The first 85 million years of avian evolution. Nature 378:349 - 355. Chien CL, Liem RK (1995) The neuronal intermediate filament, alpha-internexin is transiently expressed in amacrine cells in the developing mouse retina. Exp Eye Res 61:749-756. Choiniere JN, Xu X, Clark JM, Forster CA, Guo Y, Han F (2010) A basal alvarezsauroid theropod from the early Late Jurassic of Xinjiang, China. Science 327:571-574. Chou YH, Bischoff JR, Beach D, Goldman RD (1990) Intermediate filament reorganization during mitosis is mediated by p34cdc2 phosphorylation of vimentin. Cell 62:1063-1071. Coulombe PA, Bousquet O, Ma L, Yamada S, Wirtz D (2000) The 'ins' and 'outs' of intermediate filament organization. Trends Cell Biol 10:420-428. da Costa Calaza K, Hokoc JN, Gardino PF (2000) Neurogenesis of GABAergic cells in the chick retina. Int J Dev Neurosci 18:721-726. Dahl D, Bignami A (1986) Neurofilament phosphorylation in development. A sign of axonal maturation? Exp Cell Res 162:220-230. Doh ST, Hao H, Loh SC, Patel T, Tawil HY, Chen DK, Pashkova A, Shen A, Wang H, Cai L (2010) Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques. BMC Dev Biol 10:8. Dutting D, Gierer A, Hansmann G (1983) Self-renewal of stem cells and differentiation of nerve cells in the developing chick retina. Brain Res 312:21-32. Fekete DM, Perez-Miguelsanz J, Ryder EF, Cepko CL (1994) Clonal analysis in the chicken retina reveals tangential dispersion of clonally related cells. Dev Biol 166:666-682. Fischer AJ, Omar G (2005) Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in Muller glia in the chicken retina. J Comp Neurol 484:1-14. Fliegner KH, Kaplan MP, Wood TL, Pintar JE, Liem RK (1994) Expression of the gene for the neuronal intermediate filament protein alpha-internexin coincides with the onset of neuronal differentiation in the developing rat nervous system. J Comp Neurol 342:161-173. Fliegner KH, Liem RK (1991) Cellular and molecular biology of neuronal intermediate filaments. Int Rev Cytol 131:109-167. Foisner R, Traub P, Wiche G (1991) Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc Natl Acad Sci U S A 88:3812-3816. Fuchs E, Weber K (1994) Intermediate filaments: structure, dynamics, function, and disease. Annu Rev Biochem 63:345-382. Gardino PF, dos Santos RM, Hokoc JN (1993) Histogenesis and topographical distribution of tyrosine hydroxylase immunoreactive amacrine cells in the developing chick retina. Brain Res Dev Brain Res 72:226-236. Gervasi C, Szaro BG (1997) Sequence and expression patterns of two forms of the middle molecular weight neurofilament protein (NF-M) of Xenopus laevis. Brain Res Mol Brain Res 48:229-242. Gill FB (2006) Ornithology. Chapter 7, Unit 186-189. Gill SR, Wong PC, Monteiro MJ, Cleveland DW (1990) Assembly properties of dominant and recessive mutations in the small mouse neurofilament (NF-L) subunit. J Cell Biol 111:2005-2019. Glasgow E, Druger RK, Fuchs C, Lane WS, Schechter N (1994) Molecular cloning of gefiltin (ON1): serial expression of two new neurofilament mRNAs during optic nerve regeneration. EMBO J 13:297-305. Grant P, Pant HC (2000) Neurofilament protein synthesis and phosphorylation. J Neurocytol 29:843-872. Heins S, Wong PC, Muller S, Goldie K, Cleveland DW, Aebi U (1993) The rod domain of NF-L determines neurofilament architecture, whereas the end domains specify filament assembly and network formation. J Cell Biol 123:1517-1533. Hering H, Kroger S (1996) Formation of synaptic specializations in the inner plexiform layer of the developing chick retina. J Comp Neurol 375:393-405. Herrmann H, Aebi U (2000) Intermediate filaments and their associates: multi-talented structural elements specifying cytoarchitecture and cytodynamics. Curr Opin Cell Biol 12:79-90. Hirokawa N, Glicksman MA, Willard MB (1984) Organization of mammalian neurofilament polypeptides within the neuronal cytoskeleton. J Cell Biol 98:1523-1536. Hokoc JN, Ventura AL, Gardino PF, De Mello FG (1990) Developmental immunoreactivity for GABA and GAD in the avian retina: possible alternative pathway for GABA synthesis. Brain Res 532:197-202. Hughes WF, LaVelle A (1974) On the synaptogenic sequence in the chick retina. Anat Rec 179:297-301. Kaplan MP, Chin SS, Fliegner KH, Liem RK (1990) Alpha-internexin, a novel neuronal intermediate filament protein, precedes the low molecular weight neurofilament protein (NF-L) in the developing rat brain. J Neurosci 10:2735-2748. Knabe W, Washausen S, Happel N, Kuhn HJ (2007) Development of starburst cholinergic amacrine cells in the retina of Tupaia belangeri. J Comp Neurol 502:584-597. Lee MK, Cleveland DW (1996) Neuronal intermediate filaments. Annu Rev Neurosci 19:187-217. Lendahl U, Zimmerman LB, McKay RD (1990) CNS stem cells express a new class of intermediate filament protein. Cell 60:585-595. Li BS, Daniels MP, Pant HC (2001) Integrins stimulate phosphorylation of neurofilament NF-M subunit KSP repeats through activation of extracellular regulated-kinases (Erk1/Erk2) in cultured motoneurons and transfected NIH 3T3 cells. J Neurochem 76:703-710. Liem RK (1990) Neuronal intermediate filaments. Curr Opin Cell Biol 2:86-90. Liem RK (1993) Molecular biology of neuronal intermediate filaments. Curr Opin Cell Biol 5:12-16. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402-408. Meller K (1984) The ultrastructure of the developing inner and outer segments of the photoreceptors of chick embryo retina as revealed by the rapid-freezing and deep-etching techniques. Anat Embryol (Berl) 169:141-150. Meller K, Tetzlaff W (1976) Scanning electron microscopic studies on the development of the chick retina. Cell Tissue Res 170:145-159. Nixon RA, Shea TB (1992) Dynamics of neuronal intermediate filaments: a developmental perspective. Cell Motil Cytoskeleton 22:81-91. Nixon RA, Sihag RK (1991) Neurofilament phosphorylation: a new look at regulation and function. Trends Neurosci 14:501-506. Oblinger MM, Wong J, Parysek LM (1989) Axotomy-induced changes in the expression of a type III neuronal intermediate filament gene. J Neurosci 9:3766-3775. Pachter JS, Liem RK (1984) The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve. Dev Biol 103:200-210. Pachter JS, Liem RK (1985) alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-1322. Partin AW, Briggman JV, Subong EN, Szaro R, Oreper A, Wiesbrock S, Meyer J, Coffey DS, Epstein JI (1997) Preliminary immunohistochemical characterization of a monoclonal antibody (PRO:4-216) prepared from human prostate cancer nuclear matrix proteins. Urology 50:800-808. Perrin FE, Boisset G, Docquier M, Schaad O, Descombes P, Kato AC (2005) No widespread induction of cell death genes occurs in pure motoneurons in an amyotrophic lateral sclerosis mouse model. Hum Mol Genet 14:3309-3320. Prada C, Puga J, Perez-Mendez L, Lopez R, Ramirez G (1991) Spatial and Temporal Patterns of Neurogenesis in the Chick Retina. Eur J Neurosci 3:559-569. Rios H, Brusco A, Pecci Saavedra J (1997) Development of serotoninergic chick retinal neurons. Int J Dev Neurosci 15:729-738. Romer AS (1967) Major steps in vertebrate evolution. Science 158:1629-1637. Schwartz ML, Shneidman PS, Bruce J, Schlaepfer WW (1990) Axonal dependency of the postnatal upregulation in neurofilament expression. J Neurosci Res 27:193-201. Shaw G, Weber K (1982) Differential expression of neurofilament triplet proteins in brain development. Nature 298:277-279. Shin DH, Lee KS, Lee E, Cho SS, Kim J, Kim JW, Kwon BS, Lee HW, Lee WJ (2003) The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina. Neuroscience Letters 342:167-170. Sihag RK, Inagaki M, Yamaguchi T, Shea TB, Pant HC (2007) Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments. Exp Cell Res 313:2098-2109. Spence SG, Robson JA (1989) An autoradiographic analysis of neurogenesis in the chick retina in vitro and in vivo. Neuroscience 32:801-812. Szaro BG, Lee VM, Gainer H (1989) Spatial and temporal expression of phosphorylated and non-phosphorylated forms of neurofilament proteins in the developing nervous system of Xenopus laevis. Brain Res Dev Brain Res 48:87-103. Szaro BG, Strong MJ (2010) Post-transcriptional control of neurofilaments: New roles in development, regeneration and neurodegenerative disease. Trends Neurosci 33:27-37. Tajika M, Yamamoto K, Mekada A, Kani K, Okabe H (2004) Neuronal intermediate filament in the developing rat retina. Acta Histochemica Et Cytochemica 37:95-99. Tesser P, Jones PS, Schechter N (1986) Elevated levels of retinal neurofilament mRNA accompany optic nerve regeneration. J Neurochem 47:1235-1243. Torelli S, Sogos V, Marzilli MA, Datri M, Gremo F (1989) Developmental Expression of Intermediate Filament Proteins in the Chick-Embryo Retina - Invivo and Invitro Comparison. Experimental Biology 48:187-196. Yuan A, Rao MV, Sasaki T, Chen Y, Kumar A, Veeranna, Liem RK, Eyer J, Peterson AC, Julien JP, Nixon RA (2006) Alpha-internexin is structurally and functionally associated with the neurofilament triplet proteins in the mature CNS. J Neurosci 26:10006-10019. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24620 | - |
| dc.description.abstract | α-internexin 是一種神經元中間絲蛋白。在過去的研究中，它在發育中的囓齒類動物視網膜的表現經常和神經中間絲蛋白三合體(neurofilament triplet proteins)一起做比較。 α-internexin在發育中的小鼠視網膜會短暫地表現在無軸突細胞(amacrine cells)。在成體中，只會分布在視神經(optic nerve fibers)和水平細胞(horizontal cells)中。資料顯示α-internexin可能與發育中視網膜內神經連結可塑性有有關。由於α-internexin過去在雞視網膜中的表現尚未被探討，本研究主要目的在瞭解雞視網膜發育過程中，α-internexin和神經元中間絲蛋白在時間和空間的變化分布。
本論文利用即時定量聚合酶連鎖反應、西方墨點轉漬與免疫組織化學染色等方法研究α-internexin核酸表現與蛋白質表現量與分布。在雞胚時期第八天，α-internexin分布在神經節細胞(ganglion cell)上，接著在雞胚時期第十四天開始分布在無軸突細胞和水平細胞，並持續表達到胚胎時期第二十天。α-internexin在成體中則主要分布在視神經和無軸突細胞。除此之外，低分子量神經元中間絲蛋白與高分子量神經元中間絲蛋白在胚胎時期會和α-internexin共同出現在神經節細胞及視神經與無軸突細胞，成體時期更會分布在水平細胞上，顯示神經元中間絲蛋白可部分彌補α-internexin的功能。 本論文的結果顯示α-internexin會表現在發育中的雞視網膜，並且隨著時間的推衍而分布在不同的神經細胞中。我們認為α-internexin未來可以應用成為研究視網膜中神經細胞的特定標記物。 | zh_TW |
| dc.description.abstract | α-internexin is one of the neuronal intermediate filament proteins, together with neurofilament (NF) triplet proteins have been studied in developing rodent retina. α-internexin displayed transient expression in amacrine cells during development, and mainly localized in the optic nerve fibers, horizontal cell processes of adult mice. It has been suggested that α-internexin may play a role in neuroplasticity during development. Since the distribution of α-internexin in chicken retina has not yet been investigated, we aimed to discover the spatial and temporal distribution patterns of α-internexin and neurofilament NFL/NFH in developing chicken retina.
The RNA expression of α-internexin and NFH was examined by Real-Time Quantitative PCR. The proteins levels of α-internexin, NFL and NFH in developing retina was examined by Western blot. And the distribution of α-internexin together with NFL, NFH, parvalbumin and NeuN in chicken retina were examined by immunohistochemistry. The immunoreactivity of α-internexin was detected in ganglions cell at embryonic day 8 (E8), then appeared in soma and processes of amacrine cells as well as horizontal cells E14 onward. In adult, the immunoreactivity of α-internexin was detected mainly in the optic nerve fibers, soma and processes of amacrine cells, yet the immunoreactivity in horizontal cells could not be detected. Besides, neurofilament proteins were observed to co-localize with α-internexin in ganglion cells and amacrine cells in developing retina, and were found to appear in outer plexiform layer of adult chicken retina. This result might point to the replacement of α-internexin with neurofilament proteins in adult chicken retina. Our result revealed that differential distribution patterns of α-internexin could be found in the neurons of developing chicken retina. We suggest that α-internexin could be one of good neuronal markers in the study of chicken retina. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:33:36Z (GMT). No. of bitstreams: 1 ntu-100-R98446002-1.pdf: 22623869 bytes, checksum: dfc4c5553041afa531a5f8acffe7e079 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員會審定書
誌謝i 中文摘要:ii Abstract:iii Contents: v Chapter 1 Introduction:1 Chapter 2 Materials and Methods:9 Chapter 3 Results:14 Chapter 4 Discussion:21 Reference:28 Figure Ligands:34 | |
| dc.language.iso | en | |
| dc.title | 神經中間絲蛋白α-internexin在雞視網膜的表現 | zh_TW |
| dc.title | The neuronal intermediate filament α-internexin expression in chicken retina | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 盧國賢(Kuo-Shyan Lu),賴旗俊(Chi-Chun Lai) | |
| dc.subject.keyword | α 型神經中間絲蛋白,神經元中間絲蛋白,雞視網膜, | zh_TW |
| dc.subject.keyword | α-internexin,neuronal intermediate filament proteins,chicken retina, | en |
| dc.relation.page | 87 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-07-27 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 解剖學暨生物細胞學研究所 | zh_TW |
| 顯示於系所單位： | 解剖學暨細胞生物學科所 | |
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