請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24195完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭彥彬 | |
| dc.contributor.author | Ching-Chin Yang | en |
| dc.contributor.author | 楊靜欽 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:18:11Z | - |
| dc.date.copyright | 2005-08-18 | |
| dc.date.issued | 2005 | |
| dc.date.submitted | 2005-07-29 | |
| dc.identifier.citation | Akagi, K., M. Sano, et al. (2003). 'Involvement of toxicity as an early event in urinary bladder carcinogenesis induced by phenethyl isothiocyanate, benzyl isothiocyanate, and analogues in F344 rats.' Toxicol Pathol 31(4): 388-96.
Ashkenazi A, Dixit VM. Death receptors: signaling and modulation. Science 1998;281:1305–8. Barecki-Roach, M., E. J. Wang, et al. (2003). 'Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein.' J Pharm Pharmacol 55(9): 1251-7. Barillari, J., D. Gueyrard, et al. (2001). 'Barbarea verna as a source of 2-phenylethyl glucosinolate, precursor of cancer chemopreventive phenylethyl isothiocyanate.' Fitoterapia 72(7): 760-4. Bennett BL, Sasaki DT, Murray BW, et al SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.. Proc Natl Acad Sci USA, 98: 13681-6, 2001. Bonnesen, C., I. M. Eggleston, et al. (2001). 'Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines.' Cancer Res 61(16): 6120-30. Boone, C. W., G. D. Stoner, et al. (2000). 'Quantitative grading of rat esophageal carcinogenesis using computer-assisted image tile analysis.' Cancer Epidemiol Biomarkers Prev 9(5): 495-500. Boysen, G., P. M. Kenney, et al. (2003). 'Effects of benzyl isothiocyanate and 2-phenethyl isothiocyanate on benzo[a]pyrene and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone metabolism in F-344 rats.' Carcinogenesis 24(3): 517-25. Budihardjo I, Oliver H, Lutter M, Luo X, Wang X. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol 1999;15:269–90. Canistro, D., C. D. Croce, et al. (2004). 'Genetic and metabolic effects of gluconasturtiin, a glucosinolate derived from cruciferae.' Mutat Res 545(1-2): 23-35. Carmella, S. G., A. Borukhova, et al. (1997). 'Analysis of human urine for pyridine-N-oxide metabolites of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a tobacco-specific lung carcinogen.' Cancer Epidemiol Biomarkers Prev 6(2): 113-20. Chen, Y. H., H. J. Dai, et al. (2003). 'Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages.' Planta Med 69(8): 696-700. Chen, Y. R., J. Han, et al. (2002). 'Phenylethyl isothiocyanate induces apoptotic signaling via suppressing phosphatase activity against c-Jun N-terminal kinase.' J Biol Chem 277(42): 39334-42. Chen, Y. R., W. Wang, et al. (1998). 'Molecular mechanisms of c-Jun N-terminal kinase-mediated apoptosis induced by anticarcinogenic isothiocyanates.' J Biol Chem 273(3): 1769-75. Chiao, J. W., F. Chung, et al. (2000). 'Modulation of growth of human prostate cancer cells by the N-acetylcysteine conjugate of phenethyl isothiocyanate.' Int J Oncol 16(6): 1215-9. Chiao, J. W., H. Wu, et al. (2004). 'Ingestion of an isothiocyanate metabolite from cruciferous vegetables inhibits growth of human prostate cancer cell xenografts by apoptosis and cell cycle arrest.' Carcinogenesis 25(8): 1403-8. Chung, F. L., C. C. Conaway, et al. (2000). 'Chemoprevention of colonic aberrant crypt foci in Fischer rats by sulforaphane and phenethyl isothiocyanate.' Carcinogenesis 21(12): 2287-91. Chung FL, Morse MA, Eklind KI, Lewis J. Quantitation of human uptake of the anticarcinogen phenethyl isothiocyanate after a watercress meal. Cancer Epidemiol Biomarkers Prev 1992;1:383–8. Conaway, C. C., D. Jiao, et al. (1999). 'Disposition and pharmacokinetics of phenethyl isothiocyanate and 6-phenylhexyl isothiocyanate in F344 rats.' Drug Metab Dispos 27(1): 13-20. Conaway, C. C., J. Krzeminski, et al. (2001). 'Decomposition rates of isothiocyanate conjugates determine their activity as inhibitors of cytochrome p450 enzymes.' Chem Res Toxicol 14(9): 1170-6. Dahl, E. L. and R. T. Mulcahy (2001). 'Cell-type specific differences in glutamate cysteine ligase transcriptional regulation demonstrate independent subunit control.' Toxicol Sci 61(2): 265-72. Dingley, K. H., E. A. Ubick, et al. (2003). 'Effect of dietary constituents with chemopreventive potential on adduct formation of a low dose of the heterocyclic amines PhIP and IQ and phase II hepatic enzymes.' Nutr Cancer 46(2): 212-21. Dong, Z. (2000). 'Effects of food factors on signal transduction pathways.' Biofactors 12(1-4): 17-28. Futakuchi, M., M. Hirose, et al. (1998). 'Inhibition of DMBA-initiated rat mammary tumour development by 1-O-hexyl-2,3,5-trimethylhydroquinone, phenylethyl isothiocyanate, and novel synthetic ascorbic acid derivatives.' Eur J Cancer Prev 7(2): 153-9. Gerhauser, C., K. Klimo, et al. (2003). 'Mechanism-based in vitro screening of potential cancer chemopreventive agents.' Mutat Res 523-524: 163-72. Goosen, T. C., U. M. Kent, et al. (2000). 'Inactivation of cytochrome P450 2B1 by benzyl isothiocyanate, a chemopreventative agent from cruciferous vegetables.' Chem Res Toxicol 13(12): 1349-59. Green DR, Reed JC. Mitochondria and apoptosis. Science 1998;281:1309–12. Gross-Steinmeyer, K., P. L. Stapleton, et al. (2004). 'Phytochemical-induced changes in gene expression of carcinogen-metabolizing enzymes in cultured human primary hepatocytes.' Xenobiotica 34(7): 619-32. Hartter, S., G. Tybring, et al. (2002). 'The N-demethylation of the doxepin isomers is mainly catalyzed by the polymorphic CYP2C19.' Pharm Res 19(7): 1034-7. Hecht SS, Chung FL, Richie JP, et al. Effects of watercress consumption on metabolism of a tobacco-specific lung carcinogen in smokers. Cancer Epidemiol Biomarkers Prev 1995;4:877–84. Hecht, S. S. (1996). 'Chemoprevention of lung cancer by isothiocyanates.' Adv Exp Med Biol 401: 1-11. Hecht, S. S. (1997). 'Approaches to chemoprevention of lung cancer based on carcinogens in tobacco smoke.' Environ Health Perspect 105 Suppl 4: 955-63. Hecht, S. S. (1999). 'Chemoprevention of cancer by isothiocyanates, modifiers of carcinogen metabolism.' J Nutr 129(3): 768S-774S. Hecht, S. S., S. G. Carmella, et al. (1999). 'Effects of watercress consumption on urinary metabolites of nicotine in smokers.' Cancer Epidemiol Biomarkers Prev 8(10): 907-13. Hecht SS. (2000). Inhibition of carcinogenesis by isothiocyanates. Drug Metab Rev;32:395–411. Hecht, S. S., P. M. Kenney, et al. (2000). 'Effects of phenethyl isothiocyanate and benzyl isothiocyanate, individually and in combination, on lung tumorigenesis induced in A/J mice by benzo[a]pyrene and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.' Cancer Lett 150(1): 49-56. Hecht, S. S., P. Upadhyaya, et al. (2002). 'Inhibition of lung tumorigenesis in A/J mice by N-acetyl-S-(N-2-phenethylthiocarbamoyl)-L-cysteine and myo-inositol, individually and in combination.' Carcinogenesis 23(9): 1455-61. Hengartner MO. (2000). The biochemistry of apoptosis. Nature;407:770–6 Hirose, M., T. Yamaguchi, et al. (1998). 'Strong promoting activity of phenylethyl isothiocyanate and benzyl isothiocyanate on urinary bladder carcinogenesis in F344 male rats.' Int J Cancer 77(5): 773-7. Hu, K. and M. E. Morris (2004). 'Effects of benzyl-, phenethyl-, and alpha-naphthyl isothiocyanates on P-glycoprotein- and MRP1-mediated transport.' J Pharm Sci 93(7): 1901-11. Hu, R., B. R. Kim, et al. (2003). 'The roles of JNK and apoptotic signaling pathways in PEITC-mediated responses in human HT-29 colon adenocarcinoma cells.' Carcinogenesis 24(8): 1361-7. Hudson, T. S., P. S. Carlton, et al. (2001). 'Investigation of the enhancement of N-nitrosomethylbenzylamine-induced esophageal tumorigenesis by 6-phenylhexyl isothiocyanate.' Cancer Lett 162(1): 19-26. Hudson, T. S., G. D. Stoner, et al. (2005). 'Comparison of phenethyl and 6-phenylhexyl isothiocyanate-induced toxicity in rat esophageal cell lines with and without glutathione depletion.' Toxicol Lett 155(3): 427-36. Itoh, N., Y. Tsujimoto, and S. Nagata. 1993. Effect of bcl-2 on Fas-mediated cell death. J. Immunol. 151:621-627. Iwai, K. T. Miyawaki, T. Takizawa, A. Konno, K. Ohta, A. Yachie, H. Seki, and N. Taniguchi. (1994). Differential expression of bcl-2 and susceptibility to anti-Fas-mediated cell death in peripheral blood lymphocytes, monocytes, and neutrophils. Blood 84:1201-1208 Ji, Y. and M. E. Morris (2003). 'Determination of phenethyl isothiocyanate in human plasma and urine by ammonia derivatization and liquid chromatography-tandem mass spectrometry.' Anal Biochem 323(1): 39-47. Ji, Y. and M. E. Morris (2004). 'Effect of organic isothiocyanates on breast cancer resistance protein (ABCG2)-mediated transport.' Pharm Res 21(12): 2261-9. Jiao, D., T. J. Smith, et al. (1997). 'Chemopreventive activity of thiol conjugates of isothiocyanates for lung tumorigenesis.' Carcinogenesis 18(11): 2143-7. Johnson, C. R., J. Chun, et al. (2004). 'Intrinsic cytotoxicity and chemomodulatory actions of novel phenethylisothiocyanate sphingoid base derivatives in HL-60 human promyelocytic leukemia cells.' J Pharmacol Exp Ther 309(2): 452-61. Kassie, F. and S. Knasmuller (2000). 'Genotoxic effects of allyl isothiocyanate (AITC) and phenethyl isothiocyanate (PEITC).' Chem Biol Interact 127(2): 163-80. Kassie, F., B. Laky, et al. (2003). 'Effects of garden and water cress juices and their constituents, benzyl and phenethyl isothiocyanates, towards benzo(a)pyrene-induced DNA damage: a model study with the single cell gel electrophoresis/Hep G2 assay.' Chem Biol Interact 142(3): 285-96. Keum, Y. S., E. D. Owuor, et al. (2003). 'Involvement of Nrf2 and JNK1 in the activation of antioxidant responsive element (ARE) by chemopreventive agent phenethyl isothiocyanate (PEITC).' Pharm Res 20(9): 1351-6. Kong, A. N., E. Owuor, et al. (2001). 'Induction of xenobiotic enzymes by the MAP kinase pathway and the antioxidant or electrophile response element (ARE/EpRE).' Drug Metab Rev 33(3-4): 255-71. Kong, A. N., R. Yu, et al. (2000). 'Signal transduction events elicited by natural products: role of MAPK and caspase pathways in homeostatic response and induction of apoptosis.' Arch Pharm Res 23(1): 1-16. Kong, A. N., R. Yu, et al. (1998). 'Differential activation of MAPK and ICE/Ced-3 protease in chemical-induced apoptosis. The role of oxidative stress in the regulation of mitogen-activated protein kinases (MAPKs) leading to gene expression and survival or activation of caspases leading to apoptosis.' Restor Neurol Neurosci 12(2-3): 63-70. Kuang, Y. F. and Y. H. Chen (2004). 'Induction of apoptosis in a non-small cell human lung cancer cell line by isothiocyanates is associated with P53 and P21.' Food Chem Toxicol 42(10): 1711-8. Kurt W. (1999).Kohn Molecular Interaction Map of the Mammalian Cell Cycle Control and DNA Repair Systems Vol. 10, Issue 8,; 2703-2734. Leclercq, I., J. P. Desager, et al. (1998). 'Inhibition of chlorzoxazone metabolism, a clinical probe for CYP2E1, by a single ingestion of watercress.' Clin Pharmacol Ther 64(2): 144-9. Lee JC, Laydon JT, McDonnell PC, et al A protein kinase involved in the regulation of inflammatory cytokine biosynthesis.. Nature, 372: 739-46, 199 Liebes, L., C. C. Conaway, et al. (2001). 'High-performance liquid chromatography-based determination of total isothiocyanate levels in human plasma: application to studies with 2-phenethyl isothiocyanate.' Anal Biochem 291(2): 279-89. Matthiessen, J. N. and M. A. Shackleton (2000). 'Advantageous attributes of larval whitefringed weevil, Naupactus leucoloma (Coleoptera: Curculionidae) for bioassaying soil fumigants, and responses to pure and plant-derived isothiocyanates.' Bull Entomol Res 90(4): 349-55. Moonen, H. J., Y. E. Dommels, et al. (2004). 'Effects of polyunsaturated fatty acids on prostaglandin synthesis and cyclooxygenase-mediated DNA adduct formation by heterocyclic aromatic amines in human adenocarcinoma colon cells.' Mol Carcinog 40(3): 180-8. Moran, J. A., E. L. Dahl, et al. (2002). 'Differential induction of mafF, mafG and mafK expression by electrophile-response-element activators.' Biochem J 361(Pt 2): 371-7. Moreno, R. L., T. Goosen, et al. (2001). 'Differential effects of naturally occurring isothiocyanates on the activities of cytochrome P450 2E1 and the mutant P450 2E1 T303A.' Arch Biochem Biophys 391(1): 99-110. Morris, C. R., S. C. Chen, et al. (2004). 'Inhibition by allyl sulfides and phenethyl isothiocyanate of methyl-n-pentylnitrosamine depentylation by rat esophageal microsomes, human and rat CYP2E1, and Rat CYP2A3.' Nutr Cancer 48(1): 54-63. Morse, M. A. (1998). 'Inhibition of NNK-induced lung tumorigenesis by modulators of NNK activation.' Exp Lung Res 24(4): 595-604. Morse, M. A., J. Lu, et al. (1999). 'Metabolism of N-nitrosobenzylmethylamine by human cytochrome P-450 enzymes.' J Toxicol Environ Health A 58(7): 397-411. Murphy, S. E., L. M. Johnson, et al. (2001). 'Consumption of watercress fails to alter coumarin metabolism in humans.' Drug Metab Dispos 29(6): 786-8. Nakajima, M., R. Yoshida, et al. (2001). 'Inhibition and inactivation of human cytochrome P450 isoforms by phenethyl isothiocyanate.' Drug Metab Dispos 29(8): 1110-3. Nakamura, Y., Y. Morimitsu, et al. (2000). 'A glutathione S-transferase inducer from papaya: rapid screening, identification and structure-activity relationship of isothiocyanates.' Cancer Lett 157(2): 193-200. Neo, J. C., P. Rose, et al. (2005). 'beta-Phenylethyl isothiocyanate mediated apoptosis: a proteomic investigation of early apoptotic protein changes.' Proteomics 5(4): 1075-82. Niazi Shahabi, H., F. Bergquist, et al. (2003). 'An investigation of dopaminergic metabolites in the striatum and in the substantia nigra in vivo utilising radiolabelled L-DOPA and high performance liquid chromatography: a new approach in the search for transmitter metabolites.' Neuroscience 120(2): 425-33. Nishikawa, A., F. Furukawa, et al. (2004). 'Potent chemopreventive agents against pancreatic cancer.' Curr Cancer Drug Targets 4(4): 373-84. Nishikawa, A., M. A. Morse, et al. (2003). 'Inhibitory effects of 2-mercaptoethane sulfonate and 6-phenylhexyl isothiocyanate on urinary bladder tumorigenesis in rats induced by N-butyl-N-(4-hydroxybutyl)nitrosamine.' Cancer Lett 193(1): 11-6. Noriyuki Miyoshi, Koji Uchida, Toshihiko Osawa and Yoshimasa Nakamura (2004), A Link between Benzyl Isothiocyanate-Induced Cell Cycle Arrest and Apoptosis: Involvement of Mitogen-Activated Protein Kinases in the Bcl-2 Phosphorylation Cancer Research; 64, 2134-2142. Ogawa, K., M. Futakuchi, et al. (1998). 'Stage and organ dependent effects of 1-O-hexyl-2,3,5-trimethylhydroquinone, ascorbic acid derivatives, n-heptadecane-8-10-dione and phenylethyl isothiocyanate in a rat multiorgan carcinogenesis model.' Int J Cancer 76(6): 851-6. Ogawa, K., M. Hirose, et al. (2001). 'Dose-dependent promotion by phenylethyl isothiocyanate, a known chemopreventer, of two-stage rat urinary bladder and liver carcinogenesis.' Nutr Cancer 40(2): 134-9. Palaniswamy, U. R., R. J. McAvoy, et al. (2003). 'Ontogenic variations of ascorbic acid and phenethyl isothiocyanate concentrations in watercress (Nasturtium officinale R.Br.) leaves.' J Agric Food Chem 51(18): 5504-9. Perocco, P., R. Iori, et al. (2002). 'In vitro induction of benzo(a)pyrene cell-transforming activity by the glucosinolate gluconasturtiin found in cruciferous vegetables.' Cancer Lett 184(1): 65-71. Powolny, A., K. Takahashi, et al. (2003). 'Induction of GADD gene expression by phenethylisothiocyanate in human colon adenocarcinoma cells.' J Cell Biochem 90(6): 1128-39. Pullar, J. M., S. J. Thomson, et al. (2004). 'The chemopreventive agent phenethyl isothiocyanate sensitizes cells to Fas-mediated apoptosis.' Carcinogenesis 25(5): 765-72. Richter, E. and A. R. Tricker (2002). 'Effect of nicotine, cotinine and phenethyl isothiocyanate on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) metabolism in the Syrian golden hamster.' Toxicology 179(1-2): 95-103. Rose, P., J. S. Armstrong, et al. (2005). 'Beta-phenylethyl isothiocyanate mediated apoptosis; contribution of Bax and the mitochondrial death pathway.' Int J Biochem Cell Biol 37(1): 100-19. Rose, P., K. Faulkner, et al. (2000). '7-Methylsulfinylheptyl and 8-methylsulfinyloctyl isothiocyanates from watercress are potent inducers of phase II enzymes.' Carcinogenesis 21(11): 1983-8. Rose, P., M. Whiteman, et al. (2003). 'beta-Phenylethyl isothiocyanate-mediated apoptosis in hepatoma HepG2 cells.' Cell Mol Life Sci 60(7): 1489-503. Rose, P., Y. K. Won, et al. (2005). 'Beta-phenylethyl and 8-methylsulphinyloctyl isothiocyanates, constituents of watercress, suppress LPS induced production of nitric oxide and prostaglandin E2 in RAW 264.7 macrophages.' Nitric Oxide 12(4): 237-43. Rushmore, T. H. and A. N. Kong (2002). 'Pharmacogenomics, regulation and signaling pathways of phase I and II drug metabolizing enzymes.' Curr Drug Metab 3(5): 481-90. Shabany, K., P. C. Chiu, et al. (2002). 'Rapid in vivo assay for topical oral cancer chemopreventive agents.' Int J Oncol 21(1): 159-64. Solt, D. B., K. Chang, et al. (2003). 'Phenethyl isothiocyanate inhibits nitrosamine carcinogenesis in a model for study of oral cancer chemoprevention.' Cancer Lett 202(2): 147-52. Son, H. Y., A. Nishikawa, et al. (2000). 'Modifying effects of 4-phenylbutyl isothiocyanate on N-nitrosobis(2-oxopropyl)amine-induced tumorigenesis in hamsters.' Cancer Lett 160(2): 141-7. Staack, R., S. Kingston, et al. (1998). 'A comparison of the individual and collective effects of four glucosinolate breakdown products from brussels sprouts on induction of detoxification enzymes.' Toxicol Appl Pharmacol 149(1): 17-23. Sticha, K. R., P. M. Kenney, et al. (2002). 'Effects of benzyl isothiocyanate and phenethyl isothiocyanate on DNA adduct formation by a mixture of benzo[a]pyrene and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in A/J mouse lung.' Carcinogenesis 23(9): 1433-9. Sticha, K. R., M. E. Staretz, et al. (2000). 'Effects of benzyl isothiocyanate and phenethyl isothiocyanate on benzo[a]pyrene metabolism and DNA adduct formation in the A/J mouse.' Carcinogenesis 21(9): 1711-9. Stoner, G. D., L. A. Kresty, et al. (1999). 'Isothiocyanates and freeze-dried strawberries as inhibitors of esophageal cancer.' Toxicol Sci 52(2 Suppl): 95-100. Susin SA, Lorenzo HK, Zamzami N, et al. (1999) Molecular characterization of mitochondrial apoptosis-inducing factors. Nature;397:441–6. Takagi, H., M. Shibutani, et al. (2005). 'Limited tumor-initiating activity of phenylethyl isothiocyanate by promotion with sodium L-ascorbate in a rat two-stage urinary bladder carcinogenesis model.' Cancer Lett 219(2): 147-53. Thapliyal, R. and G. B. Maru (2001). 'Inhibition of cytochrome P450 isozymes by curcumins in vitro and in vivo.' Food Chem Toxicol 39(6): 541-7. Thornberry N, Lazebnick Y. (1998) Caspases: enemies within. Science;281:1312–6 Tseng, E., A. Kamath, et al. (2002). 'Effect of organic isothiocyanates on the P-glycoprotein- and MRP1-mediated transport of daunomycin and vinblastine.' Pharm Res 19(10): 1509-15. Tseng, E., E. A. Scott-Ramsay, et al. (2004). 'Dietary organic isothiocyanates are cytotoxic in human breast cancer MCF-7 and mammary epithelial MCF-12A cell lines.' Exp Biol Med (Maywood) 229(8): 835-42. Uematsu, Y., K. Hirata, et al. (2002). '[Determination of isothiocyanates and related compounds in mustard extract and horseradish extract used as natural food additives].' Shokuhin Eiseigaku Zasshi 43(1): 10-7. Van der Logt, E. M., H. M. Roelofs, et al. (2004). 'Effects of dietary anticarcinogens and nonsteroidal anti-inflammatory drugs on rat gastrointestinal UDP-glucuronosyltransferases.' Anticancer Res 24(2B): 843-9. Van Lieshout, E. M., M. M. Bedaf, et al. (1998). 'Effects of dietary anticarcinogens on rat gastrointestinal glutathione S-transferase theta 1-1 levels.' Carcinogenesis 19(11): 2055-7. Van Lieshout, E. M., M. P. Ekkel, et al. (1998). 'Effects of dietary anticarcinogens on rat gastrointestinal glutathione peroxidase activity.' Oncol Rep 5(4): 959-63. Visanji, J. M., S. J. Duthie, et al. (2004). 'Dietary isothiocyanates inhibit Caco-2 cell proliferation and induce G2/M phase cell cycle arrest, DNA damage, and G2/M checkpoint activation.' J Nutr 134(11): 3121-6. Wallig, M. A., S. Kingston, et al. (1998). 'Induction of rat pancreatic glutathione S-transferase and quinone reductase activities by a mixture of glucosinolate breakdown derivatives found in Brussels sprouts.' Food Chem Toxicol 36(5): 365-73. Wei MC, Zong WX, Cheng EH, et al. (2001) Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science;292:727–30. Witschi, H., I. Espiritu, et al. (2002). 'Expression of cyclin D1/2 in the lungs of strain A/J mice fed chemopreventive agents.' Carcinogenesis 23(2): 289-94. Wolf BB, Green DR. (1999) Suicidal tendencies: apoptotic cell death by caspase family proteinases. J Biol Chem;274:20049–52. Woodle, E. S., and H. R. Kulkarni. (1998). Programmed cell death. Transplantation 65:681-691 Xiao, D., S. Choi, et al. (2005). 'Role of mitogen-activated protein kinases in phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells.' Mol Carcinog 43(3): 130-40. Xiao, D., C. S. Johnson, et al. (2004). 'Proteasome-mediated degradation of cell division cycle 25C and cyclin-dependent kinase 1 in phenethyl isothiocyanate-induced G2-M-phase cell cycle arrest in PC-3 human prostate cancer cells.' Mol Cancer Ther 3(5): 567-75. Xiao, D. and S. V. Singh (2002). 'Phenethyl isothiocyanate-induced apoptosis in p53-deficient PC-3 human prostate cancer cell line is mediated by extracellular signal-regulated kinases.' Cancer Res 62(13): 3615-9. Xiao, D., Y. Zeng, et al. (2005). 'Caspase-dependent apoptosis induction by phenethyl isothiocyanate, a cruciferous vegetable-derived cancer chemopreventive agent, is mediated by Bak and Bax.' Clin Cancer Res 11(7): 2670-9. Xiong, X., W. Chen, et al. (2003). '[Effects of ursolic acid on liver-protection and bile secretion].' Zhong Yao Cai 26(8): 578-81. Xu, C., C. Y. Li, et al. (2005). 'Induction of phase I, II and III drug metabolism/transport by xenobiotics.' Arch Pharm Res 28(3): 249-68. Xu, C., G. Shen, et al. (2005). 'Suppression of NF-kappaB and NF-kappaB-regulated gene expression by sulforaphane and PEITC through IkappaBalpha, IKK pathway in human prostate cancer PC-3 cells.' Oncogene 24(28): 4486-95. Xu, K. and P. J. Thornalley (2000). 'Antitumour activity of sphingoid base adducts of phenethyl isothiocyanate.' Bioorg Med Chem Lett 10(1): 53-4. Xu, K. and P. J. Thornalley (2000). 'Studies on the mechanism of the inhibition of human leukaemia cell growth by dietary isothiocyanates and their cysteine adducts in vitro.' Biochem Pharmacol 60(2): 221-31. Xu, K. and P. J. Thornalley (2001). 'Involvement of glutathione metabolism in the cytotoxicity of the phenethyl isothiocyanate and its cysteine conjugate to human leukaemia cells in vitro.' Biochem Pharmacol 61(2): 165-77. Xu, K. and P. J. Thornalley (2001). 'Signal transduction activated by the cancer chemopreventive isothiocyanates: cleavage of BID protein, tyrosine phosphorylation and activation of JNK.' Br J Cancer 84(5): 670-3. Yang, C. S. (2001). 'Inhibition of carcinogenesis and toxicity by dietary constituents.' Adv Exp Med Biol 500: 541-50. Yang, Y. M., C. C. Conaway, et al. (2002). 'Inhibition of benzo(a)pyrene-induced lung tumorigenesis in A/J mice by dietary N-acetylcysteine conjugates of benzyl and phenethyl isothiocyanates during the postinitiation phase is associated with activation of mitogen-activated protein kinases and p53 activity and induction of apoptosis.' Cancer Res 62(1): 2-7. Yu, R., S. Mandlekar, et al. (1998). 'Chemopreventive isothiocyanates induce apoptosis and caspase-3-like protease activity.' Cancer Res 58(3): 402-8. Zhang Y, Talalay P. (1994). Anticarcinogenic activities of organic isothiocyanates: chemistry and mechanisms. Cancer Res;54:1976–81s. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24195 | - |
| dc.description.abstract | 根據衛生署民國九十三年的癌症登記報告指出，口腔癌在臺灣男性十大癌症中死亡率與發生率皆位居第四位。儘管近年在診斷及治療之科技上有不少進步，但口腔癌病人之五年存活率並未有明顯改變 。因此，需要一個更好的治療方法來改善整體存活率及生活品質。 流行病學研究指出，多吃蔬菜與水果，可以降低胃癌、食道癌、肺癌、口腔癌等腫瘤的發生率。 進一步研究發現，十字花科蔬菜中含有各種的有機異硫氰酸鹽(-N=C=S, ITC)類，具有抑制化學致癌作用。 食用乙基異硫氰酸酯（Phenylethyl Isothiocyanate, PEITC）的小鼠和大鼠可減少皮膚、直腸、口腔致癌物處理而引起癌瘤形成的機率。 但其可能的作用機制至今尚未明瞭。本研究在以人類口腔癌細胞株SAS探討PEITC對口腔癌細胞的影響，並進一步了解其可能的機制。 我們發現， 以7.5-15 μM PEITC處理SAS 細胞，可以明顯抑制其細胞生長(p<0.05)，且具有劑量依賴性 (dose-dependent) 的毒殺效應 (IC50= 7.5 μM)。 藉由流式細胞儀分析細胞之細胞週期結果顯示，以7.5 uM PEITC處理SAS 細胞，可增加G2/M細胞與sub G0細胞 (apoptotic cells)數目的百分比。而DAPI染色、cytochrome C 的釋放，及PARP的活化等，更證實PEITC引起人類口腔癌細胞死亡的方式為細胞凋亡。 在SAS細胞株中，PEITC的處理會促使p53蛋白在細胞核中表現以及p53的下游基因如Bax也會隨時間而增加，減少造成抑制細胞死亡的BcL2蛋白的表現，但並不會影響 BcL-xL 蛋白質之表現。 Fas 以及TRAIL receptor 2 (亦稱DR5) 的表現也隨著PEITC處理的時間有增加的趨勢增加。 PEITC 亦可以同時活化caspase 8 和 caspase 9 ，在 SAS 細胞的培養液中加入 caspase 8和 caspase 9的抑制劑 (Z-LEHD-FMK; Z-IETD-FMK)， 皆能減低PEITC引起的細胞凋亡，且有加成效果。 因此PEITC可藉由粒線體及死亡接受器兩條路徑造成口腔癌細胞的凋亡。另外，西方墨點分析與G2/M 相關蛋白質結果發現, PEITC會增加細胞中細胞週期調控之p21、p27 蛋白質之表現，但並不會影響 Cdk1、cyclinB1 蛋白質之表現。
進一步實驗證明PEITC會激發JNK（c-Jun NH2-termianl kinase）及ERK （Extra-cellular regulation kinase）的活性， JNK 的抑制劑SP600125 及ERK 的抑制劑 PD98059可抑制PEITC所引起的細胞凋亡。因此初步認為MAPK kinase的成員對於PEITC造成人類口腔癌細胞的凋亡也扮演了重要角色。對於PEITC是否可當作口腔癌抗癌藥物則需要再以更精確的動物實驗及臨床試驗作更深入的評估其抗癌的功效。 | zh_TW |
| dc.description.abstract | Oral cancer is the fourth leading cause of cancer-related deaths in male population in Taiwan. Despite recent advances in radiotherapy and chemotherapy, the survival of patients with oral cancer has not improved significantly. Continued investigation of new chemotherapeutic agents is thus needed. Recent studies have shown that feeding phenylethyl isothiocyanate (PEITC) inhibited carcinogen-induced mouse oral carcinogenesis. However, the mechanisms by which it inhibits oral carcinogenesis are not well understood.
The present study examined the effects of PEITC on proliferation, cell cycle and apoptosis of oral cancer cell lines SAS using trypan blue assay, flow cytometry analysis, DAPI assay and Western blot analysis. PEITC significantly inhibited the proliferation of SAS oral cancer cell lines in a dose-dependent manner with a 50% inhibition concentration (IC50) of PEITC about 7.5 μM. DNA flow cytometric analysis showed that PEITC treatment induced a G2/M arrest. PEITC treatment also caused significant apoptosis of SAS cells as evidenced by DAPI staining, increase of cytochorme C release and cleavage of PARP. These results indicate that the inhibitory effects of PEITC on oral carcinogenesis may be related to the G2/M phase arrest and induction of apoptosis. The PEITC induced cell cycle arrest was associated with the increase of p21, p27 protein, but not the protein levels of Cdk1 and cyclin B1. We also found PEITC enhanced the expression of p53 and Bax in SAS cells. In addition, PEITC decreased the expression of Bcl-2 in SAS. PEITC treatment also induced the expression of death receptors, Fas and DR5. Furthermore, PEITC could induce the activation of caspase 8 and caspase 9 in SAS cells. The PEITC-induced apoptosis was significantly attenuated in the presence of specific inhibitors of caspase-8 and caspase-9. These results suggest that apoptosis occurred via both the mitochondria mediated and death receptor mediated pathway. The MAPK family of serine/threonine kinases, including extracellular signal-regulated kinase1/2 (ERK1/2), c-jun N-terminal kinase1/2/3 (JNK1/2/3), and p38 MAPK play an important role in cell proliferation and apoptosis in response to different stimuli. We found JNK and ERK were activated shortly after PEITC treatment in SAS cells. In addition, JNK inhibitor SP600125 and the ERK inhibitor PD98059 suppressed apoptosis induced by PEITC. These results indicate that MAPKs involve in apoptosis induction by PEITC in human oral cancer cells. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:18:11Z (GMT). No. of bitstreams: 1 ntu-94-R92450017-1.pdf: 2016189 bytes, checksum: 8ecdaef3a6d2ba8bb3b7f08f10648c3a (MD5) Previous issue date: 2005 | en |
| dc.description.tableofcontents | 中文摘要 1
Abstract 3 Chapter 1 7 INTRODUCTION 7 Oral cancer 7 PEITC 9 Cell cycle 12 regulation of cell cycle 13 Apoptosis 15 Regulation of apoptosis 17 MAPK signaling cascade 26 Chapter 2 28 MATERIALS AND METHODS 28 Cell culture 28 Cytosolic protein extraction 28 Trypan blue exclusion 29 Flow cytometry 29 Western blot 30 Immunohistochemistry 32 DAPI stain 32 Cell death detectionPLUS 33 Chapter 3 34 RESULTS 34 PEITC caused dose-dependent growth inhibition in oral cancer cell line SAS 34 PEITC induced G2/M cell cycle arrest and apoptosis in oral cancer cell line SAS 34 PEITC induces cytochrome c release and caspase activation 36 PEITC induces an increase in p53 protein and down-regulates the expression of Bcl-2 37 PEITC treatment induces Fas, DR5 and Bid expression 37 PEITC phosphorylate MAPK kinase protein family………………………38 ERK Activation Contributed to PEITC Induced Apoptosis in SAS Cell…39 Effects of PEITC treatment on Cell Cycle Regulatory Proteins 40 Chapter 4 56 DISSCUSION 56 Future researches and perspectives………………………………………..64 Chapter 5 66 REFERENCES 66 | |
| dc.language.iso | en | |
| dc.subject | 細胞週期 | zh_TW |
| dc.subject | 異硫氰酸苯乙酯 | zh_TW |
| dc.subject | 細胞凋亡 | zh_TW |
| dc.subject | 口腔癌 | zh_TW |
| dc.subject | PEITC | en |
| dc.subject | G2/M cell cycle arrest | en |
| dc.subject | MAPK kinase | en |
| dc.subject | apoptosis | en |
| dc.subject | SAS cell | en |
| dc.title | PEITC做為未來口腔癌化學預防與治療藥物的發展潛力 | zh_TW |
| dc.title | Study on the Potential Usage of phenethyl isothiocynate for the Treatment of Oral Cancer | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 93-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 郭生興,蕭宏昇 | |
| dc.subject.keyword | 異硫氰酸苯乙酯,細胞凋亡,口腔癌,細胞週期, | zh_TW |
| dc.subject.keyword | PEITC,SAS cell,apoptosis,MAPK kinase,G2/M cell cycle arrest, | en |
| dc.relation.page | 78 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2005-08-01 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
| 顯示於系所單位： | 口腔生物科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-94-1.pdf 未授權公開取用 | 1.97 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。