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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 郭彥彬(Mark Yen-Ping Kuo) | |
dc.contributor.author | Shim-Jie Lin | en |
dc.contributor.author | 林世杰 | zh_TW |
dc.date.accessioned | 2021-06-08T05:16:23Z | - |
dc.date.copyright | 2011-10-03 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-07-29 | |
dc.identifier.citation | Abdulghani, J., and El-Deiry, W. S. (2010). TRAIL receptor signaling and therapeutics. Expert Opin Ther Targets 14, 1091-1108.
Adhami, V. M., Aziz, M. H., Reagan-Shaw, S. R., Nihal, M., Mukhtar, H., and Ahmad, N. (2004). Sanguinarine causes cell cycle blockade and apoptosis of human prostate carcinoma cells via modulation of cyclin kinase inhibitor-cyclin-cyclin-dependent kinase machinery. Mol Cancer Ther 3, 933-940. Ahmad, N., Gupta, S., Husain, M. M., Heiskanen, K. M., and Mukhtar, H. (2000). Differential antiproliferative and apoptotic response of sanguinarine for cancer cells versus normal cells. Clin Cancer Res 6, 1524-1528. Ashkenazi, A., and Dixit, V. M. (1998). Death receptors: signaling and modulation. Science 281, 1305-1308. Babich, H., Zuckerbraun, H. L., Barber, I. B., Babich, S. B., and Borenfreund, E. (1996). Cytotoxicity of sanguinarine chloride to cultured human cells from oral tissue. Pharmacol Toxicol 78, 397-403. Bayir, H., and Kagan, V. E. (2008). Bench-to-bedside review: Mitochondrial injury, oxidative stress and apoptosis--there is nothing more practical than a good theory. Crit Care 12, 206. Becci, P. J., Schwartz, H., Barnes, H. H., and Southard, G. L. (1987). Short-term toxicity studies of sanguinarine and of two alkaloid extracts of Sanguinaria canadensis L. J Toxicol Environ Health 20, 199-208. Beutler, B., and van Huffel, C. (1994). Unraveling function in the TNF ligand and receptor families. Science 264, 667-668. Bodmer, J. L., Holler, N., Reynard, S., Vinciguerra, P., Schneider, P., Juo, P., Blenis, J., and Tschopp, J. (2000). TRAIL receptor-2 signals apoptosis through FADD and caspase-8. Nat Cell Biol 2, 241-243. Briskin, K. B., Fady, C., Wang, M., and Lichtenstein, A. (1996). Apoptotic inhibition of head and neck squamous cell carcinoma cells by tumor necrosis factor alpha. Arch Otolaryngol Head Neck Surg 122, 559-563. Caballero-George, C., Vanderheyden, P. M., Apers, S., Van den Heuvel, H., Solis, P. N., Gupta, M. P., Claeys, M., Pieters, L., Vauquelin, G., and Vlietinck, A. J. (2002). Inhibitory activity on binding of specific ligands to the human angiotensin II AT(1) and endothelin 1 ET(A) receptors: bioactive benzo[c]phenanthridine alkaloids from the root of Bocconia frutescens. Planta Med 68, 770-775. Chai, J., Du, C., Wu, J. W., Kyin, S., Wang, X., and Shi, Y. (2000). Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature 406, 855-862. Chinnaiyan, A. M., O'Rourke, K., Tewari, M., and Dixit, V. M. (1995). FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell 81, 505-512. Choi, W. Y., Jin, C. Y., Han, M. H., Kim, G. Y., Kim, N. D., Lee, W. H., Kim, S. K., and Choi, Y. H. (2009). Sanguinarine sensitizes human gastric adenocarcinoma AGS cells to TRAIL-mediated apoptosis via down-regulation of AKT and activation of caspase-3. Anticancer Res 29, 4457-4465. Choi, W. Y., Kim, G. Y., Lee, W. H., and Choi, Y. H. (2008). Sanguinarine, a benzophenanthridine alkaloid, induces apoptosis in MDA-MB-231 human breast carcinoma cells through a reactive oxygen species-mediated mitochondrial pathway. Chemotherapy 54, 279-287. Debiton, E., Madelmont, J. C., Legault, J., and Barthomeuf, C. (2003). Sanguinarine-induced apoptosis is associated with an early and severe cellular glutathione depletion. Cancer Chemother Pharmacol 51, 474-482. Ding, Z., Tang, S. C., Weerasinghe, P., Yang, X., Pater, A., and Liepins, A. (2002). The alkaloid sanguinarine is effective against multidrug resistance in human cervical cells via bimodal cell death. Biochem Pharmacol 63, 1415-1421. Eischen, C. M., Kottke, T. J., Martins, L. M., Basi, G. S., Tung, J. S., Earnshaw, W. C., Leibson, P. J., and Kaufmann, S. H. (1997). Comparison of apoptosis in wild-type and Fas-resistant cells: chemotherapy-induced apoptosis is not dependent on Fas/Fas ligand interactions. Blood 90, 935-943. Fanger, N. A., Maliszewski, C. R., Schooley, K., and Griffith, T. S. (1999). Human dendritic cells mediate cellular apoptosis via tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). J Exp Med 190, 1155-1164. Filomeni, G., Aquilano, K., Rotilio, G., and Ciriolo, M. R. (2003). Reactive oxygen species-dependent c-Jun NH2-terminal kinase/c-Jun signaling cascade mediates neuroblastoma cell death induced by diallyl disulfide. Cancer Res 63, 5940-5949. Fukuda, M., Hamao, A., Tanaka, A., Kitada, M., Suzuki, S., Kusama, K., and Sakashita, H. (2003). Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/APO2L) and its receptors expression in human squamous cell carcinoma of the oral cavity. Oncol Rep 10, 1113-1119. Fulda, S., and Debatin, K. M. (2004). Exploiting death receptor signaling pathways for tumor therapy. Biochim Biophys Acta 1705, 27-41. Giuliana, G., Pizzo, G., Milici, M. E., Musotto, G. C., and Giangreco, R. (1997). In vitro antifungal properties of mouthrinses containing antimicrobial agents. J Periodontol 68, 729-733. Godowski, K. C. (1989). Antimicrobial action of sanguinarine. J Clin Dent 1, 96-101. Green, D. R., and Ferguson, T. A. (2001). The role of Fas ligand in immune privilege. Nat Rev Mol Cell Biol 2, 917-924. Griffith, T. S., Wiley, S. R., Kubin, M. Z., Sedger, L. M., Maliszewski, C. R., and Fanger, N. A. (1999). Monocyte-mediated tumoricidal activity via the tumor necrosis factor-related cytokine, TRAIL. J Exp Med 189, 1343-1354. Gross, A., McDonnell, J. M., and Korsmeyer, S. J. (1999). BCL-2 family members and the mitochondria in apoptosis. Genes Dev 13, 1899-1911. Hannah, J. J., Johnson, J. D., and Kuftinec, M. M. (1989). Long-term clinical evaluation of toothpaste and oral rinse containing sanguinaria extract in controlling plaque, gingival inflammation, and sulcular bleeding during orthodontic treatment. Am J Orthod Dentofacial Orthop 96, 199-207. Henderson, P. J., and Lardy, H. A. (1970). Bongkrekic acid. An inhibitor of the adenine nucleotide translocase of mitochondria. J Biol Chem 245, 1319-1326. Holy, J., Lamont, G., and Perkins, E. (2006). Disruption of nucleocytoplasmic trafficking of cyclin D1 and topoisomerase II by sanguinarine. BMC Cell Biol 7, 13. Hsu, S., Singh, B., and Schuster, G. (2004). Induction of apoptosis in oral cancer cells: agents and mechanisms for potential therapy and prevention. Oral Oncol 40, 461-473. Huh, J., Liepins, A., Zielonka, J., Andrekopoulos, C., Kalyanaraman, B., and Sorokin, A. (2006). Cyclooxygenase 2 rescues LNCaP prostate cancer cells from sanguinarine-induced apoptosis by a mechanism involving inhibition of nitric oxide synthase activity. Cancer Res 66, 3726-3736. Hussain, A. R., Al-Jomah, N. A., Siraj, A. K., Manogaran, P., Al-Hussein, K., Abubaker, J., Platanias, L. C., Al-Kuraya, K. S., and Uddin, S. (2007). Sanguinarine-dependent induction of apoptosis in primary effusion lymphoma cells. Cancer Res 67, 3888-3897. Itoh, N., Yonehara, S., Ishii, A., Yonehara, M., Mizushima, S., Sameshima, M., Hase, A., Seto, Y., and Nagata, S. (1991). The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell 66, 233-243. Jang, Y. J., Park, K. S., Chung, H. Y., and Kim, H. I. (2003). Analysis of the phenotypes of Jurkat clones with different TRAIL-sensitivities. Cancer Lett 194, 107-117. Jia, L. Q., Osada, M., Ishioka, C., Gamo, M., Ikawa, S., Suzuki, T., Shimodaira, H., Niitani, T., Kudo, T., Akiyama, M., et al. (1997). Screening the p53 status of human cell lines using a yeast functional assay. Mol Carcinog 19, 243-253. Kayagaki, N., Yamaguchi, N., Nakayama, M., Kawasaki, A., Akiba, H., Okumura, K., and Yagita, H. (1999). Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity. J Immunol 162, 2639-2647. Kerr, J. F., Wyllie, A. H., and Currie, A. R. (1972). Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26, 239-257. Kischkel, F. C., Lawrence, D. A., Tinel, A., LeBlanc, H., Virmani, A., Schow, P., Gazdar, A., Blenis, J., Arnott, D., and Ashkenazi, A. (2001). Death receptor recruitment of endogenous caspase-10 and apoptosis initiation in the absence of caspase-8. J Biol Chem 276, 46639-46646. Ko, Y. C., Huang, Y. L., Lee, C. H., Chen, M. J., Lin, L. M., and Tsai, C. C. (1995). Betel quid chewing, cigarette smoking and alcohol consumption related to oral cancer in Taiwan. J Oral Pathol Med 24, 450-453. Koga, Y., Matsuzaki, A., Suminoe, A., Hattori, H., and Hara, T. (2004). Neutrophil-derived TNF-related apoptosis-inducing ligand (TRAIL): a novel mechanism of antitumor effect by neutrophils. Cancer Res 64, 1037-1043. Kuftinec, M. M., Mueller-Joseph, L. J., and Kopczyk, R. A. (1990). Sanguinaria toothpaste and oral rinse regimen clinical efficacy in short- and long-term trials. J Can Dent Assoc 56, 31-33. Kwon, B., Youn, B. S., and Kwon, B. S. (1999). Functions of newly identified members of the tumor necrosis factor receptor/ligand superfamilies in lymphocytes. Curr Opin Immunol 11, 340-345. Lee, N. K., and Lee, S. Y. (2002). Modulation of life and death by the tumor necrosis factor receptor-associated factors (TRAFs). J Biochem Mol Biol 35, 61-66. Li, J., Meyer, A. N., and Donoghue, D. J. (1997). Nuclear localization of cyclin B1 mediates its biological activity and is regulated by phosphorylation. Proc Natl Acad Sci U S A 94, 502-507. Li, L. Y., Luo, X., and Wang, X. (2001). Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 412, 95-99. Liu, X., Kim, C. N., Yang, J., Jemmerson, R., and Wang, X. (1996). Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86, 147-157. Liu, X., Yue, P., Zhou, Z., Khuri, F. R., and Sun, S. Y. (2004). Death receptor regulation and celecoxib-induced apoptosis in human lung cancer cells. J Natl Cancer Inst 96, 1769-1780. Lopus, M., and Panda, D. (2006). The benzophenanthridine alkaloid sanguinarine perturbs microtubule assembly dynamics through tubulin binding. A possible mechanism for its antiproliferative activity. FEBS J 273, 2139-2150. Loro, L. L., Vintermyr, O. K., and Johannessen, A. C. (2003). Cell death regulation in oral squamous cell carcinoma: methodological considerations and clinical significance. J Oral Pathol Med 32, 125-138. Mackraj, I., Govender, T., and Gathiram, P. (2008). Sanguinarine. Cardiovasc Ther 26, 75-83. Mahady, G. B., Pendland, S. L., Stoia, A., and Chadwick, L. R. (2003). In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis. Phytother Res 17, 217-221. Malikova, J., Zdarilova, A., and Hlobilkova, A. (2006). Effects of sanguinarine and chelerythrine on the cell cycle and apoptosis. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150, 5-12. Mitscher, L. A., Drake, S., Gollapudi, S. R., and Okwute, S. K. (1987). A modern look at folkloric use of anti-infective agents. J Nat Prod 50, 1025-1040. Nakayama, M., Kayagaki, N., Yamaguchi, N., Okumura, K., and Yagita, H. (2000). Involvement of TWEAK in interferon gamma-stimulated monocyte cytotoxicity. J Exp Med 192, 1373-1380. Notani, K., Kobayashi, S., Kondoh, K., Shindoh, M., Ferguson, M. M., and Fukuda, H. (2000). A case of Sweet's syndrome (acute febrile neutrophilic dermatosis) with palatal ulceration. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 89, 477-479. Packham, G., and Stevenson, F. K. (2005). Bodyguards and assassins: Bcl-2 family proteins and apoptosis control in chronic lymphocytic leukaemia. Immunology 114, 441-449. Reed, J. C., Jurgensmeier, J. M., and Matsuyama, S. (1998). Bcl-2 family proteins and mitochondria. Biochim Biophys Acta 1366, 127-137. Robertson, C. (2006). What are the roles of surgery, radiation therapy and chemotherapy in the treatment of oral cancer? J Can Dent Assoc 72, 529-530. Schmeller, T., Latz-Bruning, B., and Wink, M. (1997). Biochemical activities of berberine, palmatine and sanguinarine mediating chemical defence against microorganisms and herbivores. Phytochemistry 44, 257-266. Schneider, P., and Tschopp, J. (2000). Apoptosis induced by death receptors. Pharm Acta Helv 74, 281-286. Seta, C., Fujita, M., Muraki, Y., Fukuda, J., Kobayashi, S., and Haneji, T. (2000). Fas expression and Fas monoclonal antibody-induced apoptosis in a human squamous cell carcinoma cell line, SCC-25. J Oral Pathol Med 29, 271-278. Sharawy, M., Ali, A. M., and Choi, W. S. (2003). Experimental induction of anterior disk displacement of the rabbit craniomandibular joint: an immuno-electron microscopic study of collagen and proteoglycan occurrence in the condylar cartilage. J Oral Pathol Med 32, 176-184. Shimizu, S., Narita, M., and Tsujimoto, Y. (1999). Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC. Nature 399, 483-487. Shin, M. S., Kim, H. S., Lee, S. H., Park, W. S., Kim, S. Y., Park, J. Y., Lee, J. H., Lee, S. K., Lee, S. N., Jung, S. S., et al. (2001). Mutations of tumor necrosis factor-related apoptosis-inducing ligand receptor 1 (TRAIL-R1) and receptor 2 (TRAIL-R2) genes in metastatic breast cancers. Cancer Res 61, 4942-4946. Singh, B. B., Chandler, F. W., Jr., Whitaker, S. B., and Forbes-Nelson, A. E. (1998). Immunohistochemical evaluation of bcl-2 oncoprotein in oral dysplasia and carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85, 692-698. Singh, R., Mackraj, I., Naidoo, R., and Gathiram, P. (2006). Sanguinarine downregulates AT1a gene expression in a hypertensive rat model. J Cardiovasc Pharmacol 48, 14-21. Speiser, D. E., Sebzda, E., Ohteki, T., Bachmann, M. F., Pfeffer, K., Mak, T. W., and Ohashi, P. S. (1996). Tumor necrosis factor receptor p55 mediates deletion of peripheral cytotoxic T lymphocytes in vivo. Eur J Immunol 26, 3055-3060. Sprick, M. R., Rieser, E., Stahl, H., Grosse-Wilde, A., Weigand, M. A., and Walczak, H. (2002). Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8. EMBO J 21, 4520-4530. Susin, S. A., Lorenzo, H. K., Zamzami, N., Marzo, I., Snow, B. E., Brothers, G. M., Mangion, J., Jacotot, E., Costantini, P., Loeffler, M., et al. (1999). Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397, 441-446. Szawelski, R. J., and Wharton, C. W. (1981). Kinetic solvent isotope effects on the deacylation of specific acyl-papains. Proton inventory studies on the papain-catalysed hydrolyses of specific ester substrates: analysis of possible transition state structures. Biochem J 199, 681-692. Tamamoto, T., Yoshimura, H., Takahashi, A., Asakawa, I., Ota, I., Nakagawa, H., Ohnishi, K., Ohishi, H., and Ohnishi, T. (2003). Heat-induced growth inhibition and apoptosis in transplanted human head and neck squamous cell carcinomas with different status of p53. Int J Hyperthermia 19, 590-597. Thornberry, N. A., and Lazebnik, Y. (1998). Caspases: enemies within. Science 281, 1312-1316. Tur, V., van der Sloot, A. M., Reis, C. R., Szegezdi, E., Cool, R. H., Samali, A., Serrano, L., and Quax, W. J. (2008). DR4-selective tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) variants obtained by structure-based design. J Biol Chem 283, 20560-20568. van Loo, G., Schotte, P., van Gurp, M., Demol, H., Hoorelbeke, B., Gevaert, K., Rodriguez, I., Ruiz-Carrillo, A., Vandekerckhove, J., Declercq, W., et al. (2001). Endonuclease G: a mitochondrial protein released in apoptosis and involved in caspase-independent DNA degradation. Cell Death Differ 8, 1136-1142. van Loo, G., van Gurp, M., Depuydt, B., Srinivasula, S. M., Rodriguez, I., Alnemri, E. S., Gevaert, K., Vandekerckhove, J., Declercq, W., and Vandenabeele, P. (2002). The serine protease Omi/HtrA2 is released from mitochondria during apoptosis. Omi interacts with caspase-inhibitor XIAP and induces enhanced caspase activity. Cell Death Differ 9, 20-26. Villunger, A., Egle, A., Kos, M., Hartmann, B. L., Geley, S., Kofler, R., and Greil, R. (1997). Drug-induced apoptosis is associated with enhanced Fas (Apo-1/CD95) ligand expression but occurs independently of Fas (Apo-1/CD95) signaling in human T-acute lymphatic leukemia cells. Cancer Res 57, 3331-3334. Wallach, D., Varfolomeev, E. E., Malinin, N. L., Goltsev, Y. V., Kovalenko, A. V., and Boldin, M. P. (1999). Tumor necrosis factor receptor and Fas signaling mechanisms. Annu Rev Immunol 17, 331-367. Weerasinghe, P., Hallock, S., Tang, S. C., and Liepins, A. (2001). Role of Bcl-2 family proteins and caspase-3 in sanguinarine-induced bimodal cell death. Cell Biol Toxicol 17, 371-381. Wilson, K. M., and Milan, M. A. (1995). Age differences in the formation of equivalence classes. J Gerontol B Psychol Sci Soc Sci 50, P212-218. Wolff, J., and Knipling, L. (1993). Antimicrotubule properties of benzophenanthridine alkaloids. Biochemistry 32, 13334-13339. Wu, X., Lippman, S. M., Lee, J. J., Zhu, Y., Wei, Q. V., Thomas, M., Hong, W. K., and Spitz, M. R. (2002). Chromosome instability in lymphocytes: a potential indicator of predisposition to oral premalignant lesions. Cancer Res 62, 2813-2818. Yagita, H., Takeda, K., Hayakawa, Y., Smyth, M. J., and Okumura, K. (2004). TRAIL and its receptors as targets for cancer therapy. Cancer Sci 95, 777-783. Zou, H., Li, Y., Liu, X., and Wang, X. (1999). An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem 274, 11549-11556. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24120 | - |
dc.description.abstract | 在台灣,口腔癌為一常見癌症,且發生率和死亡率都不斷攀升,根據衛生署統計,口腔癌自1991年起至今,一直都為癌症十大死因之一。近年來,雖然整體醫療技術不斷進步,但口腔癌病人之五年存活率未有顯著改善。因此,迫切需要新的口腔癌診斷與治療的方法,來改善病人的存活率與生活品質。近幾年研究指出,血根鹼(sanguinarine)可抑制胰腺癌、攝護腺癌、皮膚表皮癌及大腸癌細胞生長或產生細胞凋亡。因此本研究以人類口腔癌細胞株SAS及Ca9-22來探討血根鹼對口腔癌細胞的影響,並進一步了解其可能的機制及血根鹼是否可當作口腔癌抗癌藥物。結果顯示,以sanguinarine處理SAS及Ca9-22 細胞,可以明顯抑制細胞生長,且濃度愈高或作用時間愈長,抑制效應就愈明顯 (SAS與Ca9-22 之IC50 分別為1.1 μM及1.2 μM )。西方墨點分析顯示血根鹼增加SAS與Ca9-22細胞中DR4,DR5與 t-bid蛋白質量的表現,進而活化pro-caspase-8與pro-caspase-9,表示血根鹼主要經由活化外生性細胞凋亡路徑,然後誘發內生性細胞凋亡路徑產生細胞凋亡。更進一步發現在SAS與Ca9-22細胞中加入自由基(ROS)的抑制劑,N-acetylcysteine(NAC)後可降低sanguinarine所誘發的細胞凋亡反應,顯示ROS在sanguinarine引起的人類口腔癌細胞細胞凋亡中扮演了重要的角色。 西方墨點法分析發現NAC可降低SAS與Ca9-22細胞中DR4和DR5 蛋白量的表現,可能因此降低sanguinarine所誘發的細胞凋亡 。
低毒性濃度(SAS處理0.55μM和Ca9-22處理0.6μM)的血根鹼協同5ng/ml的TRAIL對口腔癌細胞細胞凋亡有加乘的效果。對於血根鹼是否可當作口腔癌抗癌藥物則需要再以更精確的動物實驗及臨床試驗作更深入的評估其抗癌的功效。 | zh_TW |
dc.description.abstract | Oral cancer has been one of the top ten leading causes of death from cancer since 1991 in Taiwan. Despite significant advance in cancer treatment, the overall five-year survival for patients of oral cancer is still lowest among major cancers. As traditional treatments fail to cure oral cancer, urgent demand for efficient chemotherapeutic agents to improve survival and quality of life has motivated this study.
Sanguinarine has been shown to selectively induce tumor apoptosis in several types of cancers and is regarded as a promising chemotherapeutic agent. In this study, we showed that sanguinarine significantly suppressed the growth of oral cancer SAS and Ca9-22 cells in the time-dependent and dose-dependent manner. Western blotting revealed that sanguinarine induced apoptosis in these cells and showed sanguinarine increased DR4, DR5 protein expression and activated caspase-8 and -9. Subtoxic concentrations of sanguinarine sensitize two TRAIL resistant human oral cancer cells. The apoptosis induced by the combined treatment of TRAIL and sanguinarine was mainly achieved through activation of DR5/TRAIL and DR4/TRAIL apoptotic pathways.Combined treatment of sanguinarine and TRAIL may be used as a new promising therapy for oral cancer. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T05:16:23Z (GMT). No. of bitstreams: 1 ntu-100-R98450009-1.pdf: 2357818 bytes, checksum: 38bf7e75546cabaaadff864462d7fe8f (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 中文摘要 1
Abstract 2 第一章 導論 3 1-1 口腔癌流行病學 3 1-2 口腔癌的病理學 3 1-3 口腔癌的治療 4 1-4 血根鹼(Sanguinarine)之藥性 5 1-5 Sanguinarine之抗腫瘤機制 6 1-6 細胞凋亡(Apoptosis) 7 1-7 細胞凋亡之分子機制 8 1-8 Bcl-2基因家族 10 1-9 TNF receptor與death receptor 11 第二章 材料與方法 14 2-1 細胞株培養 14 2-2 細胞存活率試驗 (MTT assay) 15 2-3 西方墨點法-細胞蛋白質的測定 15 2-4 分析細胞質內Cytochrome c 18 2-5 利用TUNEL偵測細胞凋亡 18 2-6 利用Flow cytometry偵測自由基的產生 19 2-7 抑制劑之抑制作用 19 第三章 實驗結果 21 3-1 Sanguinarine抑制SAS與Ca9-22細胞生長 21 3-2 Sanguinarine誘導SAS與Ca9-22細胞凋亡反應 21 3-3 Sanguinarine誘導外生性細胞凋亡途徑的機制 22 3-4 Sanguinarine誘導內生性細胞凋亡途徑的機制 23 3-5 Sanguinarine對於自由基reactive oxygen species(ROS)的影響 24 3-6 Sanguinarine與TRAIL藥物加成作用的探討 25 第四章 討論 28 第五章 圖與附圖 33 第六章 參考文獻 57 | |
dc.language.iso | zh-TW | |
dc.title | 血根鹼與TRAIL引起人類口腔癌細胞株細胞凋亡加成作用之研究 | zh_TW |
dc.title | Sanguinarine sensitizes human oral cancer cells to TRAIL-induced apoptosis | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蕭宏昇,張正琪 | |
dc.subject.keyword | 口腔癌,血根鹼,TRAIL,細胞凋亡, | zh_TW |
dc.subject.keyword | oral cancer,sanguinarine,TRAIL,apoptosis, | en |
dc.relation.page | 66 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-07-29 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
顯示於系所單位: | 口腔生物科學研究所 |
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ntu-100-1.pdf 目前未授權公開取用 | 2.3 MB | Adobe PDF |
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