請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24026完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許金玉 | |
| dc.contributor.author | Hsiang-Tsui Wang | en |
| dc.contributor.author | 王湘翠 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:14:23Z | - |
| dc.date.copyright | 2006-08-04 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-06 | |
| dc.identifier.citation | Abu-Jawdeh, G., Comella, N., Tomita, Y., Brown, L. F., Tognazzi, K., Sokol, S. Y., and Kocher, O. (1999). Differential expression of frpHE: a novel human stromal protein of the secreted frizzled gene family, during the endometrial cycle and malignancy. Lab Invest 79, 439-447.
Agazie, Y., Ischenko, I., and Hayman, M. (2002). Concomitant activation of the PI3K-Akt and the Ras-ERK signaling pathways is essential for transformation by the V-SEA tyrosine kinase oncogene. Oncogene 21, 697-707. Almeida, M., Han, L., Bellido, T., Manolagas, S. C., and Kousteni, S. (2005). Wnt proteins prevent apoptosis of both uncommitted osteoblast progenitors and differentiated osteoblasts by beta-catenin-dependent and -independent signaling cascades involving Src/ERK and phosphatidylinositol 3-kinase/AKT. J Biol Chem 280, 41342-41351. Bafico, A., Gazit, A., Pramila, T., Finch, P. W., Yaniv, A., and Aaronson, S. A. (1999). Interaction of frizzled related protein (FRP) with Wnt ligands and the frizzled receptor suggests alternative mechanisms for FRP inhibition of Wnt signaling. J Biol Chem 274, 16180-16187. Bienz, M., and Clevers, H. (2000). Linking colorectal cancer to Wnt signaling. Cell 103, 311-320. Black, D. M., and Solomon, E. (1993). The search for the familial breast/ovarian cancer gene. Trends Genet 9, 22-26. Brown, A. M. (2001). Wnt signaling in breast cancer: have we come full circle? Breast Cancer Res 3, 351-355. Cadigan, K. M., and Nusse, R. (1996). wingless signaling in the Drosophila eye and embryonic epidermis. Development 122, 2801-2812. Caldwell, G. M., Jones, C., Gensberg, K., Jan, S., Hardy, R. G., Byrd, P., Chughtai, S., Wallis, Y., Matthews, G. M., and Morton, D. G. (2004). The Wnt antagonist sFRP1 in colorectal tumorigenesis. Cancer Res 64, 883-888. Candidus, S., Bischoff, P., Becker, K. F., and Hofler, H. (1996). No evidence for mutations in the alpha- and beta-catenin genes in human gastric and breast carcinomas. Cancer Res 56, 49-52. Cornelisse, C. J., Cornelis, R. S., and Devilee, P. (1996). Genes responsible for familial breast cancer. Pathol Res Pract 192, 684-693. Finch, P. W., He, X., Kelley, M. J., Uren, A., Schaudies, R. P., Popescu, N. C., Rudikoff, S., Aaronson, S. A., Varmus, H. E., and Rubin, J. S. (1997). Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action. Proc Natl Acad Sci U S A 94, 6770-6775. Fukui, T., Kondo, M., Ito, G., Maeda, O., Sato, N., Yoshioka, H., Yokoi, K., Ueda, Y., Shimokata, K., and Sekido, Y. (2005). Transcriptional silencing of secreted frizzled related protein 1 (SFRP 1) by promoter hypermethylation in non-small-cell lung cancer. Oncogene 24, 6323-6327. Galli, L. M., Barnes, T., Cheng, T., Acosta, L., Anglade, A., Willert, K., Nusse, R., and Burrus, L. W. (2006). Differential inhibition of Wnt-3a by Sfrp-1, Sfrp-2, and Sfrp-3. Dev Dyn 235, 681-690. He, B., Lee, A. Y., Dadfarmay, S., You, L., Xu, Z., Reguart, N., Mazieres, J., Mikami, I., McCormick, F., and Jablons, D. M. (2005). Secreted frizzled-related protein 4 is silenced by hypermethylation and induces apoptosis in beta-catenin-deficient human mesothelioma cells. Cancer Res 65, 743-748. He, T. C., Sparks, A. B., Rago, C., Hermeking, H., Zawel, L., da Costa, L. T., Morin, P. J., Vogelstein, B., and Kinzler, K. W. (1998). Identification of c-MYC as a target of the APC pathway. Science 281, 1509-1512. Herynk, M. H., Tsan, R., Radinsky, R., and Gallick, G. E. (2003). Activation of c-Met in colorectal carcinoma cells leads to constitutive association of tyrosine-phosphorylated beta-catenin. Clin Exp Metastasis 20, 291-300. Hoang, B., Moos, M., Jr., Vukicevic, S., and Luyten, F. P. (1996). Primary structure and tissue distribution of FRZB, a novel protein related to Drosophila frizzled, suggest a role in skeletal morphogenesis. J Biol Chem 271, 26131-26137. Hrzenjak, A., Tippl, M., Kremser, M. L., Strohmeier, B., Guelly, C., Neumeister, D., Lax, S., Moinfar, F., Tabrizi, A. D., Isadi-Moud, N., Zatloukal, K., Denk, H. (2004). Inverse correlation of secreted frizzled-related protein 4 and beta-catenin expression in endometrial stromal sarcomas. J Pathol 204, 19-27. Imbert, A., Eelkema, R., Jordan, S., Feiner, H., and Cowin, P. (2001). Delta N89 beta-catenin induces precocious development, differentiation, and neoplasia in mammary gland. J Cell Biol 153, 555-568. Jones, S. E., and Jomary, C. (2002). Secreted Frizzled-related proteins: searching for relationships and patterns. Bioessays 24, 811-820. Jonsson, M., Borg, A., Nilbert, M., and Andersson, T. (2000). Involvement of adenomatous polyposis coli (APC)/beta-catenin signalling in human breast cancer. Eur J Cancer 36, 242-248. Kazanov, D., Shapira, I., Pick, M., Kolker, O., Liberman, E., Deutsch, V., Strier, L., Dvory-Sobol, H., Kunik, T., and Arber, N. (2003). Oncogenic transformation of normal enterocytes by overexpression of cyclin D1. Dig Dis Sci 48, 1251-1261. King, R. J. (1993). William L. McGuire Memorial Symposium. Estrogen and progestin effects in human breast carcinogenesis. Breast Cancer Res Treat 27, 3-15. Klopocki, E., Kristiansen, G., Wild, P. J., Klaman, I., Castanos-Velez, E., Singer, G., Stohr, R., Simon, R., Sauter, G., Leibiger, H., Essers, L., Weber, B., Hermann, K., Rosenthal, A., Hartmann, A., Dahl, E. (2004). Loss of SFRP1 is associated with breast cancer progression and poor prognosis in early stage tumors. Int J Oncol 25, 641-649. Ko, J., Ryu, K. S., Lee, Y. H., Na, D. S., Kim, Y. S., Oh, Y. M., Kim, I. S., and Kim, J. W. (2002). Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer. Exp Cell Res 280, 280-287. Langston, A. A., Malone, K. E., Thompson, J. D., Daling, J. R., and Ostrander, E. A. (1996). BRCA1 mutations in a population-based sample of young women with breast cancer. N Engl J Med 334, 137-142. Lee, A. Y., He, B., You, L., Dadfarmay, S., Xu, Z., Mazieres, J., Mikami, I., McCormick, F., and Jablons, D. M. (2004a). Expression of the secreted frizzled-related protein gene family is downregulated in human mesothelioma. Oncogene 23, 6672-6676. Lee, H. S., Lee, D. C., Park, M. H., Yang, S. J., Lee, J. J., Kim, D. M., Jang, Y., Lee, J. H., Choi, J. Y., Kang, Y. K., et al. (2006). STMN2 is a novel target of beta-catenin/TCF-mediated transcription in human hepatoma cells. Biochem Biophys Res Commun 345, 1059-1067. Lee, J. L., Chang, C. J., Chueh, L. L., and Lin, C. T. (2003). Expression of secreted frizzled-related protein 2 in a primary canine mammary tumor cell line: a candidate tumor marker for mammary tumor cells. In Vitro Cell Dev Biol Anim 39, 221-227. Lee, J. L., Chang, C. J., Wu, S. Y., Sargan, D. R., and Lin, C. T. (2004b). Secreted frizzled-related protein 2 (SFRP2) is highly expressed in canine mammary gland tumors but not in normal mammary glands. Breast Cancer Res Treat 84, 139-149. Lee, J. L., Lin, C. T., Chueh, L. L., and Chang, C. J. (2004c). Autocrine/paracrine secreted Frizzled-related protein 2 induces cellular resistance to apoptosis: a possible mechanism of mammary tumorigenesis. J Biol Chem 279, 14602-14609. Lin, S. Y., Xia, W., Wang, J. C., Kwong, K. Y., Spohn, B., Wen, Y., Pestell, R. G., and Hung, M. C. (2000). Beta-catenin, a novel prognostic marker for breast cancer: its roles in cyclin D1 expression and cancer progression. Proc Natl Acad Sci U S A 97, 4262-4266. Lo, P. K., Mehrotra, J., D'Costa, A., Fackler, M. J., Garrett-Mayer, E., Argani, P., and Sukumar, S. (2006). Epigenetic Suppression of Secreted Frizzled Related Protein 1 (SFRP1) Expression in Human Breast Cancer. Cancer Biol Ther 5, 281-286. Longnecker, M. P. (1995). Alcohol consumption and risk of cancer in humans: an overview. Alcohol 12, 87-96. Melkonyan, H. S., Chang, W. C., Shapiro, J. P., Mahadevappa, M., Fitzpatrick, P. A., Kiefer, M. C., Tomei, L. D., and Umansky, S. R. (1997). SARPs: a family of secreted apoptosis-related proteins. Proc Natl Acad Sci U S A 94, 13636-13641. Morabia, A., Bernstein, M., Heritier, S., and Khatchatrian, N. (1996). Relation of breast cancer with passive and active exposure to tobacco smoke. Am J Epidemiol 143, 918-928. Moser, A. R., Hegge, L. F., and Cardiff, R. D. (2001). Genetic background affects susceptibility to mammary hyperplasias and carcinomas in Apc(min)/+ mice. Cancer Res 61, 3480-3485. Nusse, R. (1999). WNT targets. Repression and activation. Trends Genet 15, 1-3. Orford, K., Orford, C. C., and Byers, S. W. (1999). Exogenous expression of beta-catenin regulates contact inhibition, anchorage-independent growth, anoikis, and radiation-induced cell cycle arrest. J Cell Biol 146, 855-868. Pathak, D. R., and Whittemore, A. S. (1992). Combined effects of body size, parity, and menstrual events on breast cancer incidence in seven countries. Am J Epidemiol 135, 153-168. Polakis, P. (2000). Wnt signaling and cancer. Genes Dev 14, 1837-1851. Rattner, A., Hsieh, J. C., Smallwood, P. M., Gilbert, D. J., Copeland, N. G., Jenkins, N. A., and Nathans, J. (1997). A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors. Proc Natl Acad Sci U S A 94, 2859-2863. Satoh, S., Daigo, Y., Furukawa, Y., Kato, T., Miwa, N., Nishiwaki, T., Kawasoe, T., Ishiguro, H., Fujita, M., Tokino, T., Sasaki, Y., Imaoka, S., Murata, M., Shimano, T., Yamaoka, Y., Nakamura, Y.(2000). AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1. Nat Genet 24, 245-250. Schlosshauer, P. W., Brown, S. A., Eisinger, K., Yan, Q., Guglielminetti, E. R., Parsons, R., Ellenson, L. H., and Kitajewski, J. (2000). APC truncation and increased beta-catenin levels in a human breast cancer cell line. Carcinogenesis 21, 1453-1456. Shtutman, M., Zhurinsky, J., Simcha, I., Albanese, C., D'Amico, M., Pestell, R., and Ben-Ze'ev, A. (1999). The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway. Proc Natl Acad Sci U S A 96, 5522-5527. Singletary, S. E., Allred, C., Ashley, P., Bassett, L. W., Berry, D., Bland, K. I., Borgen, P. I., Clark, G., Edge, S. B., Hayes, D. F., Hughes, L. L., Hutter, R. V., Morrow, M., Page, D. L., Recht, A., Theriault, R. L., Thor, A., Weaver, D. L., Wieand, H. S., Greene, F. L. (2002). Revision of the American Joint Committee on Cancer staging system for breast cancer. J Clin Oncol 20, 3628-3636. Suzuki, H., Watkins, D. N., Jair, K. W., Schuebel, K. E., Markowitz, S. D., Chen, W. D., Pretlow, T. P., Yang, B., Akiyama, Y., Van Engeland, M., Toyota, M., Tokino, T., Hinoda, Y., Imai, K., Herman, J. G., Baylin, S. B. (2004). Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer. Nat Genet 36, 417-422. Tetsu, O., and McCormick, F. (1999). Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Nature 398, 422-426. Tsukamoto, A. S., Grosschedl, R., Guzman, R. C., Parslow, T., and Varmus, H. E. (1988). Expression of the int-1 gene in transgenic mice is associated with mammary gland hyperplasia and adenocarcinomas in male and female mice. Cell 55, 619-625. Ugolini, F., Adelaide, J., Charafe-Jauffret, E., Nguyen, C., Jacquemier, J., Jordan, B., Birnbaum, D., and Pebusque, M. J. (1999). Differential expression assay of chromosome arm 8p genes identifies Frizzled-related (FRP1/FRZB) and Fibroblast Growth Factor Receptor 1 (FGFR1) as candidate breast cancer genes. Oncogene 18, 1903-1910. Ugolini, F., Charafe-Jauffret, E., Bardou, V. J., Geneix, J., Adelaide, J., Labat-Moleur, F., Penault-Llorca, F., Longy, M., Jacquemier, J., Birnbaum, D., and Pebusque, M. J. (2001). WNT pathway and mammary carcinogenesis: loss of expression of candidate tumor suppressor gene SFRP1 in most invasive carcinomas except of the medullary type. Oncogene 20, 5810-5817. Uren, A., Reichsman, F., Anest, V., Taylor, W. G., Muraiso, K., Bottaro, D. P., Cumberledge, S., and Rubin, J. S. (2000). Secreted frizzled-related protein-1 binds directly to Wingless and is a biphasic modulator of Wnt signaling. J Biol Chem 275, 4374-4382. Van de Vijver, M. J., and Nusse, R. (1991). The molecular biology of breast cancer. Biochim Biophys Acta 1072, 33-50. Veeck, J., Niederacher, D., An, H., Klopocki, E., Wiesmann, F., Betz, B., Galm, O., Camara, O., Durst, M., Kristiansen, G., Huszka, C., Knuchel, R., Dahl, E. (2006). Aberrant methylation of the Wnt antagonist SFRP1 in breast cancer is associated with unfavourable prognosis. Oncogene. Wissmann, C., Wild, P. J., Kaiser, S., Roepcke, S., Stoehr, R., Woenckhaus, M., Kristiansen, G., Hsieh, J. C., Hofstaedter, F., Hartmann, A., Knuechel, R., Rosenthal, A., Pilarsky, C. (2003). WIF1, a component of the Wnt pathway, is down-regulated in prostate, breast, lung, and bladder cancer. J Pathol 201, 204-212. Wong, S. C., Lo, S. F., Lee, K. C., Yam, J. W., Chan, J. K., and Wendy Hsiao, W. L. (2002). Expression of frizzled-related protein and Wnt-signalling molecules in invasive human breast tumours. J Pathol 196, 145-153. Zhou, Z., Wang, J., Han, X., Zhou, J., and Linder, S. (1998). Up-regulation of human secreted frizzled homolog in apoptosis and its down-regulation in breast tumors. Int J Cancer 78, 95-99. Zi, X., Guo, Y., Simoneau, A. R., Hope, C., Xie, J., Holcombe, R. F., and Hoang, B. H. (2005). Expression of Frzb/secreted Frizzled-related protein 3, a secreted Wnt antagonist, in human androgen-independent prostate cancer PC-3 cells suppresses tumor growth and cellular invasiveness. Cancer Res 65, 9762-9770. 羅方吟 (2005). 碩士論文 (The roles of sFRP1 and sFRP2 in human breast cancer) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24026 | - |
| dc.description.abstract | Wnt訊息傳導路徑在細胞的生長和分化上都扮演重要角色，當Wnt配體(ligands)與細胞膜上的Frizzled受體(receptors)結合後，會促使細胞質中beta-catenin增加並進入細胞核中調控特定基因之轉錄，例如：c-myc、c-jun和cyclin D1等與細胞生長有關之基因。目前在許多癌細胞中都發現參與負調控Wnt訊息傳導路徑的APC (adenomatosis polyposis coli protein)或AXIN等因子有變異的情形，因而造成此路徑有不正常的活化；然而乳癌檢體中也被發現有β-catenin增加的情形，不過並無APC或AXIN等蛋白的異常，因此推測造成乳癌中Wnt訊息傳導路徑不正常活化的原因，應該發生於APC及AXIN這個複合體的上游。由於過去的研究，造成癌細胞中此訊息傳導路徑持續被活化的基因異常，往往是受體或此訊息傳導路徑中的其他成員，而非配體本身，因此突顯了在上游調控Wnt配體與細胞膜上的Frizzled受體結合的蛋白質sFRPs(secreted frizzled related proteins；包含 sFRP1至sFRP5五個成員)的重要性。我們因而有系統性的分析sFRPs在乳癌中的表現情形以及與臨床症狀的相關性，並進一步以乳癌細胞株做模式研究其分子機制。
本論文中，我們以半定量聚合酶連鎖反應(semi-quantitative RT-PCR)或是real time PCR等方法分析sFRP3、4和5在144個乳癌檢體中的表現情形，結合過去實驗室對於sFRP1和sFRP2已完成的研究，結果發現低表現量的sFRP1或sFRP4皆與乳癌的較差愈後有關，如與術後其他器官的轉移復發(systematic recurrence)具相關性，其生統分析得到的P值分別為0.0224和0.0099。進ㄧ步我們建立了sFRP1或sFRP4穩定表現的乳癌細胞株進而分析其癌化情形。結果發現無論是sFRP1或sFRP4均會減低癌細胞的生長速度，調高癌細胞對胎牛血清的需求量，甚至減少soft agar 群落的生成能力。接下去則分析細胞中與Wnt訊息傳導路徑相關分子的表現情形，結果發現在sFRP1或sFRP4的表現下會促進GSK-3beta的活性，進而beta-catenin蛋白質在細胞質和細胞核的表現量減少，因此調控轉錄的能力下降，致使其下游基因的表現，包括：c-myc和cyclin D1都有下降的情形。另外進一步以siRNA將sFRP1抑制後，則相反地會促進Wnt訊息傳導路徑的活化，以上實驗結果皆應證了sFRP1或sFRP4皆具有抑制Wnt訊息傳導路徑的活化的功能。 綜合以上的研究結果，我們提出一個重要的乳癌致癌機制，那就是sFRP1和sFRP4會抑制Wnt訊息傳導路徑的活化，若失去正常sFRP1和sFRP4的表現量時，Wnt訊息傳導路徑則會過度的活化，而使乳癌的癌化程度增加。至於sFRP1和sFRP4是否會透過其他生理功能參與調控乳癌之癌化過程，則有待深入的研究。 | zh_TW |
| dc.description.abstract | Wnt signaling pathway plays an important role in normal embryonal development of different tissues by regulating growth, apoptosis and differentiation. Binding of a particular Wnt ligand to its target Frizzled (Fz) receptor involves formation of a membrane-located complex and a key output of this process is the stabilization of intracellular beta-catenin. As a result, increase level of beta-catenin is targeted to the nucleus, and it enables transcriptional activation of a panoply of target genes, such as c-myc, c-jun, cyclin D1 and etc. Aberrant activation of Wnt pathway has been found in several cancers. For instances, APC (adenomatosis polyposis coli protein) or AXIN, which are both negative regulators of the Wnt pathway, is mutated in colorectal cancer, oral cancer, and gastric cancer. A few of APC or AXIN mutations are found in breast carcinomas, while more than 60% of human breast cancer specimens have higher beta-catenin concentration. Therefore, it is possible that, in breast cancer, Wnt signaling is activated by components of the pathway in the upstream of the Axin complex. The recent reports indicate sFRPs (secreted frizzled related proteins) are modulators of the Wnt pathways through influencing the binding of Wnts and frizzled receptors. Hence, we are interested in exploring the roles of sFRPs in breast cancer carcinogenesis.
First, we investigate the expression pattern of sFRPs in 144 human breast cancer specimens by either semi-quantitative RT-PCR or real time PCR. After statistical data analysis, lower expression level of sFRP1 or sFRP4 correlates with systematic recurrence (p=0.0224 and p= 0.0099, respectively). Ectopic expression of sFRP1 or sFRP4 in breast cancer cells has important implications in tumorigenesis, including inhibition of growth rate and soft agar colony formation. Saturation density and serum requirement assay are slightly changed. The migration activity in vitro is influenced as well. Besides, we find that expression of sFRP1 or sFRP4 in breast cancer cells attenuates Wnt signaling, including GSK-3beta activation, down-regulation of beta-catenin, inhibition of beta-catenin transcriptional activity and down-regulation of its target genes such as c-myc and cyclin D1. These data indicate that sFRP1 or sFRP4 plays an important role in negatively regulation of Wnt signaling pathway. In conclusion, we discover an important mechanism in human breast cancer carcinogenesis. The mechanism is that sFRP1 or sFRP4 inhibits Wnt signaling pathway, and loss of sFRP1 or sFRP4 will enhance breast cancer carcinogenesis. However, we don’t know if sFRP1 or sFRP4 modulates breast cancer carcinogenesis through other signaling pathway, more detail investigation will be needed. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:14:23Z (GMT). No. of bitstreams: 1 ntu-95-R93442006-1.pdf: 4287721 bytes, checksum: 01aca073450808a97f450ac7971dad98 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 謝誌 ................................................II
中文摘要 ...............................................III 英文摘要 ................................................IV 縮寫表 .................................................V 目次 ................................................VI 圖次 ...............................................VII 表次 ..............................................VIII 附錄 ................................................IX 緒 論 .................................................1 一、乳癌介紹 ........................................1 二、Wnt訊息傳導路徑(Wnt signaling pathway) .............4 三、Secreted Frizzled Related proteins(sFRPs) .............5 四、實驗目的 ........................................8 研究材料及方法 ........................................9 一、 研究材料 ........................................9 二、 研究方法 .......................................13 第一節 sFRP家族在乳癌檢體中基因表達分析 ............13 第二節 細胞培養 .......................................16 第三節 sFRP1及sFRP4表達載體的構築 .....................16 第四節 sFRP1 siRNA及sFRP4 siRNA表達載體的構築 ..20 第五節 建立穩定表達sFRP1及sFRP4的細胞株以及穩定表達sFRP1 siRNA的細胞株 .......................................21 第六節 細胞癌化程度分析( Tumorigenicity assays) ..23 第七節 Wnt pathway訊息傳導的分析 .....................25 結 果 ................................................27 ㄧ、分析sFRPs在乳癌檢體中的表現情形與臨床症狀的關聯性 ..27 二、sFRP1和sFRP4在乳癌形成過程之角色及其分子機制 ..29 討論 ................................................32 參考文獻 ................................................35 圖次 Figure 1. Maps of sFRP family 42 Figure 2. sFRP3 expression in human breast cancer cells 43 Figure 3. sFRP4 expression in human breast cancer cells 44 Figure 4. sFRP5 expression in human breast cancer cells 45 Figure 5. Ectopic expression of sFRP1 or sFRP4 in human breast cancer cell line MCF-7 46 Figure 6. Growth condition in stable expression of sFRP1 or sFRP4 in MCF-7 47 Figure 7. Soft agar colony formation assay in stable expression of sFRP1 or sFRP4 in MCF-7 48 Figure 8. Ectopic expression of sFRP1 or sFRP4 in human breast cancer cell line HBL-435 49 Figure 9. Growth condition in stable expression of sFRP1 or sFRP4 in HBL-435 50 Figure 10. Soft agar colony formation assay in stable expression of sFRP1 or sFRP4 in HBL-435 51 Figure 11. Expression of sFRP1 or sFRP4 decreases the migration activity of HBL-435 cells in a collagen I-coated membrane. 52 Figure 12. Changes of the Wnt signaling pathway in stable expression of sFRP1 or sFRP4 in MCF-7 53 Figure 13. To determine the localization of | |
| dc.language.iso | zh-TW | |
| dc.subject | 癌化分析 | zh_TW |
| dc.subject | 乳癌 | zh_TW |
| dc.subject | 術後其他器官轉移復發 | zh_TW |
| dc.subject | Wnt | en |
| dc.subject | sFRPs | en |
| dc.subject | beta-catenin | en |
| dc.title | sFRPs基因在人類乳癌中所扮演的角色 | zh_TW |
| dc.title | The roles of sFRPs in human breast cancer | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張智芬,游偉絢 | |
| dc.subject.keyword | 乳癌,術後其他器官轉移復發,癌化分析, | zh_TW |
| dc.subject.keyword | sFRPs,Wnt,beta-catenin, | en |
| dc.relation.page | 80 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2006-07-06 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 生物化學暨分子生物學研究所 | zh_TW |
| 顯示於系所單位： | 生物化學暨分子生物學科研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-95-1.pdf 未授權公開取用 | 4.19 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。