Please use this identifier to cite or link to this item:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23958Full metadata record
| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 李英周(Ying-Chou Lee) | |
| dc.contributor.author | Yen-Ta Chen | en |
| dc.contributor.author | 陳彥達 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:13:05Z | - |
| dc.date.copyright | 2006-07-25 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-14 | |
| dc.identifier.citation | Arenal A., Pimentel R., García C., Pimentel E. and Aleström P. (2004). The SV40 T antigen nuclear localization sequence enhances nuclear import of vector DNA in embryos of a crustacean (Litopenaeus schmitti). Gene 337: 71-77.
Boshart M., Weber F., Jahn G., Dorsch-Häsler K., Fleckenstein B., and Schaffner W. (1985). A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41: 521-530 Brinster R.L., Chen H.Y., Trumbauer M.E., Yagle M.K. and Palmiter R.D. (1985). Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc. Natl. Acad. Sci. USA 82: 4438-4442. Browne R.A. and Sorgeloos P. (1991). Experimental biology of cyst diapause. In Artemia Biology. CRC Press. pp. 93-118. Chen Y.L. and Tsai H.J. (1997). Effect of electroporation conditions on loach sperm for successful gene transfer and early development. Fish. Sci. 63: 527-532. Chourrout D., Guyomard R., Houdebine L.M. (1986). High efficiency gene transfer in rainbow trout (Salmo gairdneri Rich) by microinjection into egg cytoplasm. Aquaculture 51: 143– 150. Clegg J.S. and Conte F.P. (1980). A review of the cellular and developmental biology of Artemia. In The Brine Shrimp Artemia. vol.2. Universa Press. pp. 11-54. Clegg J.S. (2001). Cryptobiosis- a peculiar state of biological organization. Comp. Biochem. Physiol. B. 128: 613-624. Collas P., Husebye H., Aleström P. (1996). The nuclear localization sequence of the SV40 T antigen promotes transgene uptake and expression in zebrafish embryo nuclei. Transgenic Res. 5: 451– 458. Dhont J., Lavens P. and Sorgeloos P. (1993). Preparation and use of Artemia as food for shrimp and prawn larvae. CRC Handbook of Mariculture (2nd edition). Crustacean Aquaculture. vol. 1. CRC Press. pp. 61-93. Evjemo J.O., Coutteau P., Olsen Y. and Sorgeloos P. (1997). The stability of docosahexaenoic acid in two Artemia species following enrichment and subsequent starvation. Aquaculture 155: 135-148. Gendreau S., Lardans V., Cadoret J.P., Mialhe E. (1995). Transient expression of a luciferase reporter gene after ballistic introduction into Artemia franciscana (Crustacea) embryos. Aquaculture 133: 199-205. Ghartey-Tagoe E.B., Babbin B.A., Nusrat A., Neish A.S. and Prausnitz M.R. (2006). Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporation. Int. J. Pharm. 315: 122-133. Gómez-Chiarri M., Smith G.J., de la Fuente J., Powers D.A. (1998). Gene transfer in shellfish and algae. In Gene Transfer in Aquatic Organisms. Springer. pp. 107-125. Inoue K., Yamashit S., Hata J., Kabeno S., Asada S., Nagahisa E. and Fujita T. (1990). Electroporation as a new technique for producing transgenic fish. Cell Differ. Dev. 29: 123– 128. Keilin D. (1959). The problem of anabiosis or latent life: history and current concept. Proc. Roy. Soc. Lond. Ser. B 150: 149-191. Levine M. (2002). How insects lose their limbs. Nature 415: 848-849. Lewis D.L., DeCamillis M.A., Brunetti C.R., Halder G., Kassner V.A., Selegue J.E., Higgs S. and Carroll S.B. (1999). Ectopic gene expression and homeotic transformations in arthropods using recombinant Sindbis viruses. Current Biology 9: 1279-1287. Lisac D., Franicevic V., Veljmelka Z., Buble J., Léger P. and Sorgeloos P. (1986). International study on Artemia XLIII. The effect of live food fatty acid content on growth and survival of sea bream (Sparus aurata) larvae. In Conference of ichthyopathology in aquaculture. pp. 1-10. Li S.S., Tsai H.J. (2000). Transfer of foreign gene to giant freshwater prawn (Macrobrachium rosenbergii) by spermatophore-microinjection. Mol. Reprod. Dev. 56: 149– 154. Martinez-Lamparero A., Casero M.C., Ortiz-Caro J. and Sastre L. (2003). Regulation of promoter occupancy during activation of cryptobiotic embryos from the crustacean Artemia franciscana. J. Exp. Biol. 206: 1565-1573. Morris R.W. and Afzelius B.A. (1967). The structure of the shell and outer membranes in encysted Artemia salina embryos during cryptobiosis and development. J. Ultstruct. Res. 20: 244-259. Nambu Z., Tanaka S. and Nambu F. (2004). Influence of photoperiod and temperature on reproductive mode in the brine shrimp, Artemia franciscana. J. Exp. Zool. Comp. Exp. Biol. 301: 542-546. Ortega M.A., Díaz-Guerra M. and Sastre L. (1996). Actin gene structure in two Artemia species, A. franciscana and A.parthenogenetica. J. Mol.Evol. 43: 224-235. Pavlopoulos A. and Averof M. (2005). Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis. Proc. Natl. Acad. Sci. USA 102: 7888-7893. Ronshaugen M., McGinnis N. and McGinnis W. (2002). Hox protein mutation and macroevolution of the insect body plan. Nature 415: 914-917. Sanford J.C. (1988). The biolistic process. Trends Biotechnol. 6: 299-302. Sorgeloos P., Bossuyt E., Laviña E., Baeza-Mesa M. and Persoone G. (1977). Decapsulation of Artemia cysts: A simple technique for the improvement of the use of brine shrimp in aquaculture. Aquaculture 12: 311-315. Takagi A., Sasado T., Tamiya G., Ozato K., Wakamatsu Y., Takeshita A. and Kimura M. (1994). An efficient expression vector for transgenic medaka construction. Mol. Mar. Biol. Biotechnol. 3: 192–199. Tanguay J. A., Reyes R.C. and Clegg J.S. (2004). Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia. J. Biosci. 29: 489-501. Tate W.P. and Marshall C.J. (1991). Post-dormancy transcription and translation in the brine shrimp. In Artemia Biology. CRC Press. pp. 21-36. Tsai H.J., Tseng F.S. and Liao I.C. (1995). Electroporation of sperm to introduce foreign into the genome of loach (Misgurnus anguillicaudatus). Can. J. Fish. Aquat. Sci. 52: 776-787. Tsai H.J. (2000). Electroporated sperm mediation of a gene transfer system for finfish and shellfish. Mol. Reprod. Dev. 56: 281– 284. Tseng F.S., Tsai H.J., Liao I.C., Song Y.L. (2000). Introducing foreign DNA into tiger shrimp (Penaeus monodon) by electroporation. Theriogenology 54: 1421– 1432. Watanabe T., Oowa F., Kitajima C., and Fujta S. (1978). Nutritional quality of brine shrimp, Artemia salina, as a living feed from the viewpoint of essential fatty acid for fish. In Bulletin of the Japanese Society of Scientific Fisheries 44: 1115-1121. Wolf H., Rols M.P., Boldt E., Neumann E. and Teissie J. (1994). Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophy. J. 66: 524-531. Zelenin A.V., Alimov A.A. Barmintzev V.A., Benjimov A.O., Zelenina I.A., Krasnov A.M. and Kolesnikov V.A. (1991). The delivery of foreign genes into fertilized eggs using high-velocity microprojectiles. FEBS Lett. 287: 118-120. (Website resources) Drewes C.(2005). Quantitative Investigations of Hatching in Brine Shrimp Cysts. ABLE. http://www.eeob.iastate.edu/faculty/DrewesC/htdocs/#non-oligochaetes USGS (2001). United States Geological Survey- Great Salt Lake: http://www.usgs.gov/ (Others) 蔡奇立. (1993). Gene Transfer of Oyster (Crassostrea gigas Thunberg), hard clam and brine shrmp (Artemia salina Linnaeus) by electroporaton. 國立台灣大學漁業科學研究所養殖組碩士論文. 詹益豪. (2003). 超音波誘發豐年蝦卵活化及助長之研究. 國立中山 大學機械工程研究所碩士論文. 陳紫媖, 蘇惠美. (2005). 水產種苗的生產. 科學發展385: 1-10. 陳曜鴻, 李文志, 蔡懷楨. (1999). 九孔β-tubulin 基因的選殖及分子結構分析. 生物技術在水產養殖上應用論文集 3: 59-66. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23958 | - |
| dc.description.abstract | 甲殼類生物豐年蝦 (Artemia sp.) 的無節幼蟲，又稱為鹽水蝦，是最廣泛用於餵食養殖魚貝類幼苗的活體浮游餌料生物。藉由基因轉殖豐年蝦系統建立的優勢，其上游消費者的特性可被間接改良，例如：快速成長或抗病。此方法也許可以降低使用基因改良魚貝類的潛在風險。在此，我們發展出了一種基因轉殖方法，能有效地將外源性的去氧核醣核酸 (DNA) 導入目標物種 (Artemia sinica) 的基因體中。不同的構築質體經由電穿孔法，被導入經去殼處理後的豐年蝦休眠卵中，在2千伏特、20微秒的脈衝波長度、20次的脈衝數和在100微升的體積中DNA濃度為每微升20微克的設定條件下。在我們試驗的七個不同構築質體中，被命名為pCMV-MB-EGFPITR的DNA片段，含有被人類巨細胞病毒啟動子和稻田魚β型肌動蛋白啟動子所驅動的增強型綠螢光蛋白報導基因，能產生最強的綠螢光表現。雖然豐年蝦無節幼蟲於24小時後的孵化率 (30±1％) 顯著地低於未經處理的對照組 (61±7％)，但於孵化後72小時的綠螢光表現率為33±6％。轉殖豐年蝦親代、第一子代和第二子代的基因體DNA被萃取出來，一個含有719個鹼基對的產物，以聚合酶連鎖反應被擴增放大出來，這符合了被當作是正控制組的報導基因長度。這些證據都說明了轉殖基因確實有鑲嵌入轉殖豐年蝦的基因體中，而且可以遺傳至子代。這是第一篇報導證明了具有強螢光表現的豐年蝦轉殖品系，可以被創造出來。 | zh_TW |
| dc.description.abstract | The nauplii of crustacean Artemia, also named brine shrimp, are the most widely used live zooplankton bait for feeding larvae of aquaculture finfish and shellfish. By taking advantages of genetically transformed Artemia, some traits of the feeder can be improved indirectly such as fast-growing or disease-resistance. This approach might decrease the potential risk of using genetically modified finfish and shellfish. Here we developed a gene transfer method which enabled to introduce exogenous DNA into the genome of target species－Artemia sinica efficiently. Different DNA constructs were introduced into the decapsulated cysts of Artemia by electroporation under 2KV, 20 μsec pulse length, 20 number of pulses and a DNA concentration of 20μg/μl in a volume of 100μl. Among seven different constructions we examined, a DNA fragment, named pCMV-MB-EGFPITR, containing EGFP reporter gene driven by human cytomegalovirus (CMV) promoter and medaka β-actin promoter, produced the most intense GFP expression. Although the 24 hour-hatching rate of Artemia nauplii (30±1％) was significantly lower than untreated group (61±7％), the GFP expression rate was 33±6％ in 72 hours after hatching. Genomic DNA was extracted from the F0, F1 and F2 generation of transgenic Artemia, a 719-bp product was amplified which was correspondent with the reporter gene as a positive control, by polymerase chain reaction. These evidences suggest that transgene was integrated into the genome of transgenic Artemia and inherited to their offsprings. This is the first report which demonstrates that a transgenic line of Artemia with strong fluorescent expression can be generated. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:13:05Z (GMT). No. of bitstreams: 1 ntu-95-R93b45014-1.pdf: 3822510 bytes, checksum: cdedbc4d866f5abb12e85576c5811c6b (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 誌謝 (Acknowledgement)………………................... Ⅱ
中文摘要 (Abstract in Chinese)………………...…… 1 英文摘要 (Abstract in English)………………………. 3 前言 (Introduction)……………………………………. 4 材料與方法 (Materials and methods) ……………… 10 結果 (Results)………………………………………… 27 討論 (Discussions)…………………………………… 36 參考資料 (References)………………………………. 50 圖與表 (Figures and tables) …………................. 56 附錄 (Appendix)………………………………………. 61 | |
| dc.language.iso | zh-TW | |
| dc.subject | 去殼處理的休眠卵 | zh_TW |
| dc.subject | 甲殼動物 | zh_TW |
| dc.subject | 豐年蝦 | zh_TW |
| dc.subject | 增強型的綠螢光蛋白 | zh_TW |
| dc.subject | 電穿孔法 | zh_TW |
| dc.subject | decapsulated cyst | en |
| dc.subject | EGFP (Enhanced Green Fluorescence Protein) | en |
| dc.subject | electroporation | en |
| dc.subject | Artemia | en |
| dc.subject | crustacean | en |
| dc.title | 電穿孔法在豐年蝦之基因轉殖研究 | zh_TW |
| dc.title | Gene transfer of brine shrimp (Artemia sinica) by electroporation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 蔡懷楨(Huai-Jen Tsai) | |
| dc.contributor.oralexamcommittee | 鄭登貴(Teng-Kui Cheng),嚴宏洋(Hong-Young Yan),陳一鳴(I-Ming Chen) | |
| dc.subject.keyword | 甲殼動物,豐年蝦,去殼處理的休眠卵,電穿孔法,增強型的綠螢光蛋白, | zh_TW |
| dc.subject.keyword | crustacean,Artemia,decapsulated cyst,electroporation,EGFP (Enhanced Green Fluorescence Protein), | en |
| dc.relation.page | 66 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2006-07-17 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| Appears in Collections: | 漁業科學研究所 | |
Files in This Item:
| File | Size | Format | |
|---|---|---|---|
| ntu-95-1.pdf Restricted Access | 3.73 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.