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  1. NTU Theses and Dissertations Repository
  2. 醫學院
  3. 法醫學科所
Please use this identifier to cite or link to this item: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23687
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dc.contributor.advisor翁德怡
dc.contributor.authorHung-Wei Laien
dc.contributor.author賴泓瑋zh_TW
dc.date.accessioned2021-06-08T05:07:13Z-
dc.date.copyright2011-10-11
dc.date.issued2011
dc.date.submitted2011-06-23
dc.identifier.citation1. 何邦立, 華航B-1816名古屋空難航空醫學調查分析. 中華民國航空醫學會刊, 1996. 10(2): p. 65-71.
2. Kuo T.-L., Medico-legal evaluation of ethanol concentration in the medical data of the factual investigation of the factual investigation draft report of CAL A300-622R B1816. 1995.
3. Corry J.E.L., Possible Sources of Ethanol Ante- and Post-mortem: its Relationship to the Biochemistry and Microbiology of Decomposition. Journal of Applied Microbiology, 1978. 44(1): p. 1-56.
4. Yajima D., Motani H., Kamei K., Sato Y., Hayakawa M., and Iwase H., Ethanol production by Candida albicans in postmortem human blood samples: Effects of blood glucose level and dilution. Forensic Science International, 2006. 164(2-3): p. 116-121.
5. Clark M.A. and Jones J.W., Studies on putrefactive ethanol production. I: Lack of spontaneous ethanol production in intact human bodies. J Forensic Sci, 1982. 27(2): p. 366-371.
6. Blume P. and Lakatua D.J., The effect of microbial contamination of the blood sample on the determination of ethanol levels in serum. American Journal of Clinical Pathology, 1973. 60(5): p. 700-702.
7. Ball W. and Lichtenwalner M., Ethanol production in infected urine. N Engl J Med, 1979. 301(11): p. 614.
8. Nanikawa R., Moriya F., and Hashimoto Y., Experimental studies on the mechanism of ethanol formation in corpses. Z Rechtsmed, 1988. 101(1): p. 21-26.
9. Chang J. and Kollman S.E., The effect of temperature on the formation of ethanol by Candida albicans in blood. J Forensic Sci, 1989. 34(1): p. 105-109.
10. Amick G.D. and Habben K.H., Inhibition of ethanol production by Saccharomyces cerevisiae in human blood by sodium fluoride. J Forensic Sci, 1997. 42(4): p. 690-692.
11. Gottschalk G., Bacterial metabolism. 1986, New York: Springer-Verlag.
12. Bogusz M., Guminska M., and Markiewicz J., Studies on the formation of endogenous ethanol in blood putrefying in vitro. J Forensic Med, 1970. 17(4): p. 156-168.
13. Blackmore D.J., The bacterial production of ethyl alcohol. J Forensic Sci Soc, 1968. 8(2): p. 73-78.
14. Davis G.L., Leffert R.L., and Rantanen N.W., Putrefactive ethanol sources in postmortem tissues of conventional and germ-free mice. Archives of Pathology, 1972. 94(1): p. 71-74.
15. Caplan Y.H., Vitreous humor in the evaluation of postmortem blood ethanol concentrations. Journal of Analytical Toxicology, 1990. 14(5): p. 305.
16. D.V. C., author's response to letter. Journal of Forensic Sciences, 1994. 39(3): p. 600.
17. Bonnichsen R., Halstrom F., Moller K.O., and Theorell H., Development of ethanol in blood samples and human organs during forensic chemical practice. Acta Pharmacol Toxicol (Copenh), 1953. 9(4): p. 352-361.
18. Jones A.W., Andersson R., Sakshaug J., and Morland J., Possible formation of ethanol in postmortem blood specimens after antemortem treatment with mannitol. J Anal Toxicol, 1991. 15(3): p. 157-158.
19. Canfield D.V., Kupiec T., and Huffine E., Postmortem alcohol production in fatal aircraft accidents. J Forensic Sci, 1993. 38(4): p. 914-917.
20. Collison I.B., Elevated postmortem ethanol concentrations in an insulin-dependent diabetic. J Anal Toxicol, 2005. 29(7): p. 762-764.
21. Harrison B., Markes R., Bradley P., and Ismail I.A., A comparison of statistical techniques to evaluate the performance of the Glucometer Elite blood glucose meter. Clin Biochem, 1996. 29(6): p. 521-527.
22. MacKinnon D.T. and Henderson A.R., A laboratory assessment of the Miles Glucometer Elite blood glucose meter. Clin Biochem, 1994. 27(6): p. 501-505.
23. Gr, Nert, C, Wood, and W. G G., Comparison between the Bayer ELITE glucometer and the hexokinase method for glucose determination on the Eppendorf EPOS 5060 using capillary whole blood samples. 1995, De Gruyter: Berlin, ALLEMAGNE.
24. Macchia T., Mancinelli R., Gentili S., Lugaresi E.C., Raponi A., and Taggi F., Ethanol in biological fluids: headspace GC measurement. J Anal Toxicol, 1995. 19(4): p. 241-246.
25. Skoog, 儀器分析精華版. 2007.
26. 中華民國行政院環境保護署環境檢驗所, 環境檢驗室檢量線製備及確認指引.
27. Bartlett G.R. and Marlow A.A., Comparative effects of the different formed elements on normal human blood glycolysis. J Appl Physiol, 1953. 6(6): p. 335-347.
28. 最新微生物學. 華杏機構叢書. 2001, 台北市: 匯華.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23687-
dc.description.abstract已有文獻證實,人死後微生物可進入血液中利用血糖發酵產生乙醇,導致血中乙醇濃度升高。造成未喝酒的人,法醫卻誤判生前飲酒,影響死者的權利甚大。相關的司法實例包括臺灣高等法院民事判決97年保險上字第19號、臺灣彰化地方法院民事判決93年度保險字第9號等。故需要進行相關實驗,觀察死後血糖濃度和微生物發酵所產生乙醇濃度兩者間的關係。本研究選擇E. Coli為在體外血中進行發酵作用而產生乙醇的微生物,並進行兩次實驗,第一次實驗研究高血糖與所產生乙醇濃度的相互關係,實驗一分成三組,分別為正常血糖種菌組、高血糖種菌組、和正常血糖無種菌組(Negative control),高血糖組起始血糖濃度為1056 mg/dl。第二次實驗研究E. Coli在不同血糖濃度下所可能產生的乙醇含量。血糖濃度分為200mg/dl、300mg/dl、400mg/dl,共三組;每組四支樣品,其中三支種菌,剩下一支做為陰性對照組。每支試管含5ml全血並存放於室溫下,第一次實驗進行一個月,於第0、1、2、5、7、9、12、15、19、22、26、29天進行血糖和乙醇濃度的分析作業。第二次實驗進行兩個星期,於第0、1、2、3、4、5、7、9、11、13天進行血糖和乙醇濃度的分析作業。分析儀器使用頂空氣相層析儀及拜耳理易測血糖機進行分析並加以記錄。實驗一高血糖組於實驗開始第1天即產生平均51.16±3.54 mg/dl的乙醇,且於整個實驗中平均乙醇濃度均高於50 mg/dl;正常血糖種菌組第1天產生平均1.66±2.35 mg/dl的乙醇,整個實驗過程平均乙醇濃度最高為5.63 mg/dl;正常血糖無種菌組在整個實驗過程中未產生乙醇。實驗二結果血糖濃度200mg/dl組於第1天產生20.44±4.87 mg/dl的乙醇,300mg/dl組於第1天產生36.67±3.95 mg/dl的乙醇,400mg/dl組於第1天產生50.87±0.73 mg/dl的乙醇,整個實驗二過程中三組乙醇濃度大致維持一定,無明顯下降趨勢。從結果可推論E. Coli利用血中葡萄糖發酵產生乙醇的能力,隨起始血糖濃度越高而越強,且能夠產生超過濃度50 mg/dl的乙醇。現實狀況如死者為糖尿病病人,死後血中存在E. Coli,且未良好控制血糖的情況下有可能導致誤判。zh_TW
dc.description.provenanceMade available in DSpace on 2021-06-08T05:07:13Z (GMT). No. of bitstreams: 1
ntu-100-R95452011-1.pdf: 2672356 bytes, checksum: c16dfd627702a62a825e70c21bb404ab (MD5)
Previous issue date: 2011
en
dc.description.tableofcontents中文摘要 i
Abstract iii
第一章 緒論 1
1. 前言: 1
2. 文獻回顧: 2
3. 研究動機: 5
第二章 材料與方法 6
1. 實驗材料: 6
2. 實驗儀器設備: 6
3. 實驗方法: 7
4. 方法確效: 11
第三章 實驗結果 13
1. 方法確效: 13
2. 血糖濃度變化: 14
3. 血中乙醇濃度變化: 15
第四章 討論 16
1. 本實驗血糖變化結果與國外相關文獻結果之比較 16
2. 大腸桿菌使用葡萄糖為基質製造乙醇的能力 16
3. 不同微生物利用血糖製造乙醇能力之比較 17
4. 大腸桿菌菌量對乙醇濃度的影響 17
5. 去補體作用對乙醇濃度的影響 18
第五章 實務與展望 19
1. 本研究與法醫實務應用之關係 19
2. 未來展望 19
參考文獻 20
dc.language.isozh-TW
dc.title微生物之發酵作用與血中葡萄糖及乙醇濃度的相關性研究zh_TW
dc.titleThe relationship among microbial fermentation, blood glucose and alcohol concentrationen
dc.typeThesis
dc.date.schoolyear99-2
dc.description.degree碩士
dc.contributor.oralexamcommittee邱清華,彭福佐
dc.subject.keyword大腸桿菌,發酵,血中乙醇濃度,血糖濃度,頂空氣相層析,zh_TW
dc.subject.keywordE. coli,fermentation,ethanol,blood glucose concentration,headspace gas chromatography,en
dc.relation.page38
dc.rights.note未授權
dc.date.accepted2011-06-23
dc.contributor.author-college醫學院zh_TW
dc.contributor.author-dept法醫學研究所zh_TW
Appears in Collections:法醫學科所

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