請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23615
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭石通 | |
dc.contributor.author | Cherng-Hann Lin | en |
dc.contributor.author | 林承翰 | zh_TW |
dc.date.accessioned | 2021-06-08T05:04:45Z | - |
dc.date.copyright | 2011-02-20 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-01-25 | |
dc.identifier.citation | 劉浩雲 (2006) 耐熱番茄與不耐熱番茄中RSG與LeHsc70-1基因之分析及比較。國立台灣大學植物科學所畢業論文。
陳郁蕙 (2007) 耐熱番茄雄蕊表達基因E8-6和Clone 7之分離與分析。國立台灣大學植物科學所畢業論文。 陳顯榮 (1987) Nicotiana Plumbaginifolia 抗5-甲基色氨酸變異體之篩選及定性。國立台灣大學植物學研究所。 王怡潔 (2006) 耐熱番茄與不耐熱番茄中SAM3與SUS3基因之分析及比較。國立國立台灣大學植物科學所畢業論文。 張志豪 (2001) 酵素有機合成唾液酸衍生物。台灣大學生化科學研究所碩士論文。 蕭保元 (2004) 持續表現阿拉伯芥色胺酸合成酶可以幫助抵抗逆境。國立中央大學生命科學所碩士論文。 Barone, P., Zhang, X.H., and Widholm, J.M. (2009) Tobacco plastid transformation using the feedbackinsensitive anthranilate synthase [α]-subunit of tobacco (ASA2) as a new selectable marker. J. Exp. Bot. 60: 3195–3202. Blix, G., Svennerholm L., and Werner, I. (1952) The isolation of chondrosamine from gangliosides and from submaxillary mucin. Acta. Chem. Acand. 6: 358-362. Butelli, E., Titta, L., Giorgio, M., Mock, H.P., Matros, A., Peterek, S., Schijlen, E.G., Hall, R.D., Bovy, A.G., Luo, J., and Martin, C. (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat. Biotechnol. 26: 1301-1308. Carlson, S.E., Werkman, S.H., Tolley, E.A. (1996) Effect of long-chain ω-3 fatty acid supplementation on visual acuity and growth of preterm infants with and without bronchopulmonary dysplasia. Am. J. Clin. Nutr. 63: 687–697. Carolina, C. and Francisco, A.C. (2004) Tomato transformation and transgenic plant production. Plant Cell, Tissue and Organ Culture 76: 269–275. Cho, H.J., Brotherton, J.E., Song, H.S., and Widholm, J.M. (2000) Increasing tryptophan synthesis in a forage legume Astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) gene. Plant Physiol. 123: 1069–1076. Dale, P.J., Clarke, B., and Fontes, E.M.G. (2002) Potential for the environmental impact of transgenic crops. Nat. Biothchnol. 20: 567-574. Deikman, J., and Fischer, R.L. (1988a) Interaction of a DNA-binding factor with the 5'-flanking region of an ethylene-responsive fruit ripening gene from tomato. EMBO J. 7: 3315-3320. 49 Deikman, J., Xu, R.L., Kneissl, M.L., Ciardi, J.A., Kim, K.N., and Pelah, D. (1998b) Separation of cis elements responsive to ethylene, fruit development, and ripening in the 5'-flanking region of the ripening-related E8 gene. Plant Mol. Biol. 37: 1001-1011. Dombrecht B., Xue G.P., Sprague S.J., Kirkegaard J.A., Ross J.J., Reid J. B., Fitt G.P., Sewelam N., Schenk P.M., Manners J.M., and Kazan K. (2007) MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis. Plant cell 19: 2225-2245. Freund, R., Garcea, R., Sahli, R., and Benjamin, T.L. (1991) A single amino acid substitution in polyomavirus VP1 correlates with plaque size and hemagglutination behavior. J. Virol. 65: 350–355. Gibson, R.A. (1999) Long-chain polyunsaturated fatty acids and infant development. Lancet 354: 1919–1920. Glasgow, L.R. and Hill, R.L. (1980) Interaction of mycoplasma gallisepticum with sialylglycoproteins. Infect. Immun. 30: 353-361. Gomord, V. and Faye, L. (2004) Posttranslational modification of therapeutic proteins in plants. Curr. Opin. Plant Biol. 7: 171-181. Guo, C.T., Takahashi, T., Bukawa, W., Takahashi, N., Yagi, H., Kato, K., Hidari, K.I., Miyamoto, D., Suzuki, T., and Suzuki, Y. (2006) Edible bird's nest extract inhibits influenza virus infection. Antiviral. Res. 70: 140-146. Gubareva, L.V., Kaiser, L., and Hayden, F.G., (2000) Influenza virus neuraminidase inhibitors. Lancet 355: 827–835. Hakomori, S. (1984) Tumor-associated carbohydrate antigens. Annu. Rev. Immunol. 2: 103-126. Haldrup, A., Petersen, S., and Okkels, F. (1998) Plant xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows efective selection of transgenic plant cells using D-xylose as the selection agent. Plant Mol. Biol. 37: 287–296. Heritage, J. (2004) The fate of transgenes in the human gut. Nat. Biotechnol. 22: 170-172. Hsiao P., Sanjaya, Su R.C., A J., Silva T., and Chan M.T. (2007) Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation. Planta 225: 897-906. Jacobs, C.L., Goon, S., Yarema, K. J., Hinderlich, S., Hang, H.C., Chai, D.H., and Bertozzi, C.R. (2001) Substrate specificity of the sialic acid biosynthetic pathway. Biochemstry. 40: 12864-12874. Last, R.L., Bissinger, P.H., Mahoney, D.J., Radwanski, E.R., and Fink, G.R. (1991) Tryptophan mutants in Arabidopsis: the consequences of duplicated tryptophan 50 synthase beta genes. Plant Cell 3: 345–358. Loomes, L.M., Uemura, K., Childs, R.A., Paulson, J.C., Rogers, G.N., Scudder, P.R., Michalski, J., Hounsell, E.F., Taylor-Robinson, D., and Feizi, T. (1984) Erythrocyte receptors for mycoplasma pneumonia are sialylated oligosaccharides of Ii antigen type. Nature 307: 560-563. Maru I., Ohnishi J., Ohta Y., and Tsukada Y. (2002) Why is sialic acid attracting interest now? complete enzymatic synthesis of sialic acid with N-Acylglucosamine 2-Epimerase. J. Biosci. Bioeng. 93: 258-265. Matrosovich, M. and Klenk, H.D. (2003) Natural and synthetic sialic acid-containing inhibitors of influenza virus receptor binding. Rev. Med. Virol. 13: 85-97. Miki, B. and McHugh, S. (2004) Selection marker genes in transgenic plants: applications, alternatives and biosafety. J. Biotechnol. 170: 193-232. Morgan, B.L. and Winick, M. (1980) Effects of administration of N–acetylneuraminic acid (NANA) on brain NANA content and behavior. J. Nutr. 110: 416–424. Newburg, D.S. (1999) Human milk glycoconjugates that inhibit pathogens. Curr. Med. Chem. 6: 117–127. Oskar, E., Magnus, H., and Torgny, N. (2005) (2005) The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation. Plant Mol. Biol. 57:425–433 Pardoe, G.I. and Uhlenbruck, G. (1970) Characteristics of antigenie determinants of intact cell surfaces. J. Med. Lab. Technol. 27: 249-263. Pritchett, T.J., Brossmer, R., Rose, U., and Paulson, J.C. (1986) Recognition of monovalent sialosides by influenza virus H3 hemagglutinin. Virology 160: 502-506. Reinhard, Z., Daniel, K., Richard, S., and Friedrich, A. (2006) Sialic acid concentrations in plants are in the range of inadvertent contamination. Planta 224: 222-227. Reutter, W., Kiittgen, E., Bauer, C., and Gerok, W. (1982) Biological and significance of sialic acids. Sialic acids chemistry, metabolism and tin&ion. Cell biology monographs, 10: 263-292. Roseman, S. (1970) The synthesis of carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion. Chem. Phys. Lipids 5: 270-297. Saint-Jore-Dupas, C., Faye, L., and Gomord, V. (2007) From planta to pharma with glycosylation in the toolbox. Trends. Biotechnol. 25: 317–323. Sanjaya, Hsiao, P.Y., Su, R.C., Ko, S.S., Tong, C.G., Yang, R.Y., and Chan, M.T. (2008) Overexpression of Arabidopsis thaliana tryptophan synthase beta 1 (AtTSB1) in Arabidopsis and tomato confers tolerance to cadmium stress. Plant Cell Environ. 31: 1074-1085. Schauer, R. (2000) Achievements and challenges of sialic acid research. Glycoconj. J. 51 17: 485–499 Schauer, R. (1991) Biosynthesis and function of N- and O-substituted sialic acids. Glycobiology 1: 449-452. Schauer, R. and Kamerling, J.P. (1997) Chemistry, biochemistry and biology of sialic acids. In Glycoproteins II, ed. H Schachter, J Montreuil & JFG Vliegenthart, 19B: 243–372. Schauer, R., Kelm, S., Reuter, G. and Roggentin, P. (1995) Biochemistry and role of sialic acid. In Biology of the Sialic Acids, ed. A Rosenberg, pp 7–49. New York: Plenum Press. Stachel, S.E., Nester, E.W., and Zambryski, P.C. (1986) A plant cell factor induces Agrobacterium tumefaciens vir gene expression. Proc. Nat. Acad. Sci. 83: 379–383 Suzuki, Y., Nagao, Y., Kate, H., Matsumoto, M., Nerome, K., Nakajima, K., Nobusawa, E. (1986) Human influenza A virus hemagglutinin distinguishes sialyloligosaccharides in membrane-associated gangliosides as its receptor which mediates the adsorption and fusion processes of virus infection. Specificity for oligosaccharides and sialic acids and the sequence to which sialic acid is attached. J. Biol. Chem. 261: 17057-17061. Tanner, M.E. (2005) The enzymes of sialic acid biosynthesis. Bioorg. Chem. 33: 216-228. Varki, A. (1993) Biological roles of oligosaccharides: all of the theories are correct. Glycobiology 3: 97–130. von Itzstein, M. and Thomson, R.J. (1997) Sialic acids and sialic acid-recognising proteins: drug discovery targets and potential glycopharmaceuticals. Curr. Med. Chem. 4: 185–210. Wang B. (2009) Sialic acid is an essential nutrient for brain development and cognition Annu. Rev. Nutr. 29: 177-222. Wang, B., Yu, B., Karim, M., Hu, H., Sun, Y., McGreevy, P., Petocz, P., Held, S., and Brand-Miller J. (2007 )Dietary sialic acid supplementation improves learning and memory in piglets Am. J. Clin. Nutr. 85: 561-569. Wiebke, A. and Ralph, B. (2009) Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion. Plant Physiol. 151: 59–66. Widholm, J.M. (1972 a) Cultured Nicotiana tabacum cells with an altered anthranilate synthetase which is less sensitive to feedback inhibition. Biochim. Biophys. Acta. 261: 52-58. Widholm, J.M. (1972b) Anthranilate synthetase from 5-methyltryptophan-susceptible and -resistant cultured Daucuscarota cells. Biochim. Biophys. Acta. 279: 48–57. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23615 | - |
dc.description.abstract | 唾液酸是含有9個碳原子的酸性胺基醣類,在自然界有43種衍生物,氮原子乙醯化的唾液酸便是N-乙醯葡萄醣胺(N-acetylneuraminic acid)。N-乙醯葡萄醣胺在自然界分布最廣,並且是人體中最主要的唾液酸。分子結構上N-乙醯葡萄醣胺存在於糖蛋白(glycoprotein)和糖脂(glycolipid)上多醣的末端,是辨識細胞的重要標記;並且在人體中有65%唾液酸存在於中樞神經系統中。病源菌和病毒尌是以附著在N-乙醯葡萄醣胺的方式,而侵入人體細胞,因此病源菌辨識N-乙醯葡萄醣胺,並且附著寄主細胞,為寄主感病的第一個步驟。唾液酸可應用在降低病原菌和病毒辨識寄主細胞。唾液酸也是組成神經細胞的重要成分之一。但是N-乙醯葡萄醣胺不存在於植物體中,故本實驗以reverse transcription-polymerase chain reaction的方式由豬腎細胞中的cDNA中分離出可以合成N-乙醯葡萄醣胺的兩個基因,分別可產生酵素N-acylglucosamine 2-epimerase (AGE)和N-acetylneuraminate lyase (NAL),並同時將AGE和NAL建構在植物轉殖載體上,以農桿菌將此載體轉染至菸草中,並以載體上原有的抗hygromycin基因做為篩選標誌。目前已由HPLC分析得知,在轉殖菸草中,可以合成N-乙醯葡萄醣胺。同時也在含有AGE和NAL的植物轉殖載體上,加上阿拉伯芥的tryptophan synthase beta 1 (AtTSB1)基因作為非抗生素抗性篩選標的基因,以農桿菌將此含有AGE、NAL、和AtTSB1的載體轉染至番茄中,再以5-methyl tryptophan篩選得到轉殖番茄。目前致力於轉殖番茄以得到含有N-乙醯葡萄醣胺的機能性番茄。 | zh_TW |
dc.description.abstract | The sialic acids are a family of nine carbon α-keto acids that play a wide variety of biological roles in nature. In mammals, they are found at the distal ends of cell surface glycoconjugates, and thus are major determinants of cellular recognition and adhesion events. N-acetylneuraminic acid (Neu5Ac) is the most widespread form of sialic acid and nearly the only form found in humans. Influenza viruses recognize the Neu5Ac on the cell surface, and then bind to host cells. The application of Neu5Ac was shown to decrease the binding of pathogens to host cells. Neu5Ac is also a key component of neural tissues. However, Neu5Ac has not been found in plants. Two genes, encoding N-acylglucosamine 2-epimerase (AGE) and N-acetylneuraminate lyase (NAL), involved in synthesis of Neu5Ac were isolated from the cDNA of porcine kidney by reverse transcription-polymerase chain reaction. Vector containing both AGE and NAL genes were then constructed, and transformed into tobaccos by Agrobacterium-mediated transformation. The selection marker gene of the vector is the hygromycin-resistance gene, hptII. By HPLC analysis, Neu5Ac can be synthesized by the transgenic tobacco. Also, a non-antibiotic selection marker gene, AtTSB1, from Arabidopsis was introduced into the vector with AGE and NAL genes. The vector with AGE, NAL, and AtTSB1 was transformed into tomatoes by Agrobacterium-mediated transformation, and the transgenic tomatoes were selected by 5-methyl tryptophan. Now, the nutritional enhancement tomatoes with Neu5Ac is on progress to be produced. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T05:04:45Z (GMT). No. of bitstreams: 1 ntu-100-R97b42006-1.pdf: 1727957 bytes, checksum: 98ee7c7a33fb985e94e5e02929017429 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員審定書...........................................I
致謝....................................................II 中文摘要.................................. ............III Abstract .............................................. IV 第一章 前言 .............................................1 唾液酸 (N-acetylneuraminic acid, Neu5Ac)............ ..1 Neu5Ac合成途徑 ..................................... ..2 E8-6 啟動子 .......................................... 3 非抗生素篩選 ......................................... 4 番茄營養成分 ......................................... 5 研究目的與方向 ....................................... 6 第二章 材料與方法 ...................................... 7 一、豬腎與植物細胞RNA的萃取 .......................... 7 二、AGE基因、NAL基因與AtTSB1基因的調取 ............... 8 三、E8-6啟動子之調取 ................................. 9 四、質體構築與挑選 ................................... 9 五、轉殖番茄所用之質體構築 .......................... 11 六、農桿菌之轉型 .................................... 13 七、轉殖番茄 ........................................ 14 八、轉殖菸草 ........................................ 16 九、唾液酸的含量分析 ................................ 17 第三章 結果 ........................................... 20 一、唾液酸合成酵素基因釣取 .......................... 20 二、轉殖載體的構築 .................................. 20 三、目標基因於轉殖株中的篩選與表現 .................. 22 四、轉殖株中唾液酸含量的分析 ........................ 24 第四章 討論 ............................................26 一、三種不同轉殖載體功用 ............................ 26 二、T-DNA大小與轉殖效率的關係 ....................... 27 三、番茄轉植株死亡原因 .............................. 28 四、非轉殖株能在5MT培養基中生長的原因探討 ........... 28 五、菸草轉殖株內無法合成唾液酸原因探討 .............. 30 六、植物中合成唾液酸的未來潛力 ...................... 31 圖表 .................................................. 32 參考文獻 .............................................. 48 附錄 .................................................. 52 | |
dc.language.iso | zh-TW | |
dc.title | 以番茄與菸草為材料轉殖N-acylglucosamine 2-epimerase與N-acetylneuraminate lyase基因以生產N-acetylneuraminic acid | zh_TW |
dc.title | Transforming N-acylglucosamine 2-epimerase and N-acetylneuraminate lyase genes to produce N-acetylneuraminic acid in tomato and tobacco | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林讚標,鄭貽生,張孟基 | |
dc.subject.keyword | 唾液酸,番茄,N-乙醯葡萄醣胺,色胺酸合成酶, | zh_TW |
dc.subject.keyword | Sialic acid,tomato,N-acetylneuraminic acid (Neu5Ac),Tryptophan synthase β1 (AtTSB1), | en |
dc.relation.page | 58 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-01-25 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf 目前未授權公開取用 | 1.69 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。