請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23425
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蘇慧敏 | |
dc.contributor.author | Yung-Yu Mei | en |
dc.contributor.author | 梅永郁 | zh_TW |
dc.date.accessioned | 2021-06-08T05:01:27Z | - |
dc.date.copyright | 2011-03-03 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-12-04 | |
dc.identifier.citation | Adams MM, Oung T, Morrison JH, Gore AC (2001) Length of postovariectomy interval and age, but not estrogen replacement, regulate N-methyl-D-aspartate receptor mRNA levels in the hippocampus of female rats. Exp Neurol 170:345-356.
Arnold AP, Breedlove SM (1985) Organizational and activational effects of sex steroids on brain and behavior: a reanalysis. Horm Behav 19:469-498. Behl C (2002) Oestrogen as a neuroprotective hormone. Nat Rev Neurosci 3:433-442. Benfenati F, Valtorta F, Rubenstein JL, Gorelick FS, Greengard P, Czernik AJ (1992) Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I. Nature 359:417-420. Berchtold NC, Kesslak JP, Pike CJ, Adlard PA, Cotman CW (2001) Estrogen and exercise interact to regulate brain-derived neurotrophic factor mRNA and protein expression in the hippocampus. Eur J Neurosci 14:1992-2002. Bliss TV, Collingridge GL (1993) A synaptic model of memory: long-term potentiation in the hippocampus. Nature 361:31-39. Bourre JM, Francois M, Youyou A, Dumont O, Piciotti M, Pascal G, Durand G (1989) The effects of dietary alpha-linolenic acid on the composition of nerve membranes, enzymatic activity, amplitude of electrophysiological parameters, resistance to poisons and performance of learning tasks in rats. J Nutr 119:1880-1892. Bowen RA, Wierzbicki AA, Clandinin MT (1999) Does increasing dietary linolenic acid content increase the docosahexaenoic acid content of phospholipids in neuronal cells of neonatal rats? Pediatr Res 45:815-819. Burdge GC (2006) Metabolism of alpha-linolenic acid in humans. Prostaglandins Leukot Essent Fatty Acids 75:161-168. Calon F LG, Morihara T, Yang F, Ubeda O, Salem N Jr, Frautschy SA, Cole GM. ( 2005) Dietary n-3 polyunsaturated fatty acid depletion activates caspases and decreases NMDA receptors in the brain of a transgenic mouse model of Alzheimer's disease. Eur J Neurosci 22:617-626. Cao D KK, Kim J, Moon HS, Jun SB, Lovinger D, Kim HY. (2009) Docosahexaenoic acid promotes hippocampal neuronal development and synaptic function. J Neurochem 111:510-521. Carrie I, Clement M, de Javel D, Frances H, Bourre JM (2000) Phospholipid supplementation reverses behavioral and biochemical alterations induced by n-3 polyunsaturated fatty acid deficiency in mice. J Lipid Res 41:473-480. Chakraborty PK, Brown JL, Ruff CB, Nelson MF, Mitchell AS (1991) Effects of long-term treatment with estradiol or clomiphene citrate on bone maintenance, and pituitary and uterine weights in ovariectomized rats. J Steroid Biochem Mol Biol 40:725-729. Chung WL, Chen JJ, Su HM (2008) Fish oil supplementation of control and (n-3) fatty acid-deficient male rats enhances reference and working memory performance and increases brain regional docosahexaenoic acid levels. J Nutr 138:1165-1171. Clandinin MT (1999) Brain development and assessing the supply of polyunsaturated fatty acid. Lipids 34:131-137. Curtis J, Finkbeiner S (1999) Sending signals from the synapse to the nucleus: possible roles for CaMK, Ras/ERK, and SAPK pathways in the regulation of synaptic plasticity and neuronal growth. J Neurosci Res 58:88-95. Cyr M, Ghribi O, Di Paolo T (2000) Regional and selective effects of oestradiol and progesterone on NMDA and AMPA receptors in the rat brain. J Neuroendocrinol 12:445-452. Cyr M, Ghribi O, Thibault C, Morissette M, Landry M, Di Paolo T (2001) Ovarian steroids and selective estrogen receptor modulators activity on rat brain NMDA and AMPA receptors. Brain Res Brain Res Rev 37:153-161. Daniel JM, Hulst JL, Berbling JL (2006) Estradiol replacement enhances working memory in middle-aged rats when initiated immediately after ovariectomy but not after a long-term period of ovarian hormone deprivation. Endocrinology 147:607-614. Delion S, Chalon S, Guilloteau D, Lejeune B, Besnard JC, Durand G (1997) Age-related changes in phospholipid fatty acid composition and monoaminergic neurotransmission in the hippocampus of rats fed a balanced or an n-3 polyunsaturated fatty acid-deficient diet. J Lipid Res 38:680-689. DeMar JC, Jr., Ma K, Bell JM, Igarashi M, Greenstein D, Rapoport SI (2006) One generation of n-3 polyunsaturated fatty acid deprivation increases depression and aggression test scores in rats. J Lipid Res 47:172-180. Diaz-Veliz G, Urresta F, Dussaubat N, Mora S (1991) Effects of estradiol replacement in ovariectomized rats on conditioned avoidance responses and other behaviors. Physiol Behav 50:61-65. Dosemeci A, Albers RW (1996) A mechanism for synaptic frequency detection through autophosphorylation of CaM kinase II. Biophys J 70:2493-2501. Favrelere S, Stadelmann-Ingrand S, Huguet F, De Javel D, Piriou A, Tallineau C, Durand G (2000) Age-related changes in ethanolamine glycerophospholipid fatty acid levels in rat frontal cortex and hippocampus. Neurobiol Aging 21:653-660. Fernandez SM, Frick KM (2004) Chronic oral estrogen affects memory and neurochemistry in middle-aged female mice. Behav Neurosci 118:1340-1351. Galea LA, Wide JK, Paine TA, Holmes MM, Ormerod BK, Floresco SB (2001) High levels of estradiol disrupt conditioned place preference learning, stimulus response learning and reference memory but have limited effects on working memory. Behav Brain Res 126:115-126. Garcia-Segura LM, Azcoitia I, DonCarlos LL (2001) Neuroprotection by estradiol. Prog Neurobiol 63:29-60. Gibbs RB (1998) Levels of trkA and BDNF mRNA, but not NGF mRNA, fluctuate across the estrous cycle and increase in response to acute hormone replacement. Brain Res 810:294. Giltay EJ, Duschek EJ, Katan MB, Zock PL, Neele SJ, Netelenbos JC (2004) Raloxifene and hormone replacement therapy increase arachidonic acid and docosahexaenoic acid levels in postmenopausal women. J Endocrinol 182:399-408. Gould E, Woolley CS, Frankfurt M, McEwen BS (1990) Gonadal steroids regulate dendritic spine density in hippocampal pyramidal cells in adulthood. J Neurosci 10:1286-1291. Green P, Glozman S, Kamensky B, Yavin E (1999) Developmental changes in rat brain membrane lipids and fatty acids. The preferential prenatal accumulation of docosahexaenoic acid. J Lipid Res 40:960-966. Grodin JM, Siiteri PK, MacDonald PC (1973) Source of estrogen production in postmenopausal women. J Clin Endocrinol Metab 36:207-214. Hinds HL, Tonegawa S, Malinow R (1998) CA1 long-term potentiation is diminished but present in hippocampal slices from alpha-CaMKII mutant mice. Learn Mem 5:344-354. Holmes MM, Wide JK, Galea LA (2002) Low levels of estradiol facilitate, whereas high levels of estradiol impair, working memory performance on the radial arm maze. Behav Neurosci 116:928-934. Kanaseki T, Ikeuchi Y, Sugiura H, Yamauchi T (1991) Structural features of Ca2+/calmodulin-dependent protein kinase II revealed by electron microscopy. J Cell Biol 115:1049-1060. Lacroix L, Broersen LM, Weiner I, Feldon J (1998) The effects of excitotoxic lesion of the medial prefrontal cortex on latent inhibition, prepulse inhibition, food hoarding, elevated plus maze, active avoidance and locomotor activity in the rat. Neuroscience 84:431-442. Lin SY, Wu K, Levine ES, Mount HT, Suen PC, Black IB (1998) BDNF acutely increases tyrosine phosphorylation of the NMDA receptor subunit 2B in cortical and hippocampal postsynaptic densities. Brain Res Mol Brain Res 55:20-27. Lledo PM, Hjelmstad GO, Mukherji S, Soderling TR, Malenka RC, Nicoll RA (1995) Calcium/calmodulin-dependent kinase II and long-term potentiation enhance synaptic transmission by the same mechanism. Proc Natl Acad Sci U S A 92:11175-11179. Lu B (2003) Pro-region of neurotrophins: role in synaptic modulation. Neuron 39:735-738. Marszalek JR, Lodish HF (2005) Docosahexaenoic acid, fatty acid-interacting proteins, and neuronal function: breastmilk and fish are good for you. Annu Rev Cell Dev Biol 21:633-657. McLaughlin KJ, Bimonte-Nelson H, Neisewander JL, Conrad CD (2008) Assessment of estradiol influence on spatial tasks and hippocampal CA1 spines: evidence that the duration of hormone deprivation after ovariectomy compromises 17beta-estradiol effectiveness in altering CA1 spines. Horm Behav 54:386-395. Minami M, Kimura S, Endo T, Hamaue N, Hirafuji M, Togashi H, Matsumoto M, Yoshioka M, Saito H, Watanabe S, Kobayashi T, Okuyama H (1997) Dietary docosahexaenoic acid increases cerebral acetylcholine levels and improves passive avoidance performance in stroke-prone spontaneously hypertensive rats. Pharmacol Biochem Behav 58:1123-1129. Mora S, Dussaubat N, Diaz-Veliz G (1996) Effects of the estrous cycle and ovarian hormones on behavioral indices of anxiety in female rats. Psychoneuroendocrinology 21:609-620. Morris MC, Evans DA, Bienias JL, Tangney CC, Bennett DA, Wilson RS, Aggarwal N, Schneider J (2003) Consumption of fish and n-3 fatty acids and risk of incident Alzheimer disease. Arch Neurol 60:940-946. Nichols RA, Chilcote TJ, Czernik AJ, Greengard P (1992) Synapsin I regulates glutamate release from rat brain synaptosomes. J Neurochem 58:783-785. Nomikos GG, Spyraki C (1988) Influence of oestrogen on spontaneous and diazepam-induced exploration of rats in an elevated plus maze. Neuropharmacology 27:691-696. Okaniwa Y, Yuasa S, Yamamoto N, Watanabe S, Kobayashi T, Okuyama H, Nomura M, Nagata Y (1996) A high linoleate and a high alpha-linolenate diet induced changes in learning behavior of rats. Effects of a shift in diets and reversal of training stimuli. Biol Pharm Bull 19:536-540. Paganini-Hill A, Henderson VW (1994) Estrogen deficiency and risk of Alzheimer's disease in women. Am J Epidemiol 140:256-261. Pettit DL, Perlman S, Malinow R (1994) Potentiated transmission and prevention of further LTP by increased CaMKII activity in postsynaptic hippocampal slice neurons. Science 266:1881-1885. Rebas E, Lachowicz L, Lachowicz A (2005) Estradiol modulates the synapsins phosphorylation by various protein kinases in the rat brain under in vitro and in vivo conditions. J Physiol Pharmacol 56:39-48. Roy EJ, Wade GN (1977) Role of food intake in estradiol-induced body weight changes in female rats. Horm Behav 8:265-274. Sawai T, Bernier F, Fukushima T, Hashimoto T, Ogura H, Nishizawa Y (2002) Estrogen induces a rapid increase of calcium-calmodulin-dependent protein kinase II activity in the hippocampus. Brain Res 950:308-311. Scharfman HE, Maclusky NJ (2005) Similarities between actions of estrogen and BDNF in the hippocampus: coincidence or clue? Trends Neurosci 28:79-85. Sfikakis A, Spyraki C, Sitaras N, Varonos D (1978) Implication of the estrous cycle on conditioned avoidance behavior in the rat. Physiol Behav 21:441-446. Silva AJ, Stevens CF, Tonegawa S, Wang Y (1992) Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice. Science 257:201-206. Sinclair AJ, Begg D, Mathai M, Weisinger RS (2007) Omega 3 fatty acids and the brain: review of studies in depression. Asia Pac J Clin Nutr 16 Suppl 1:391-397. Singh M, Meyer EM, Millard WJ, Simpkins JW (1994) Ovarian steroid deprivation results in a reversible learning impairment and compromised cholinergic function in female Sprague-Dawley rats. Brain Res 644:305-312. Soderberg M, Edlund C, Kristensson K, Dallner G (1991) Fatty acid composition of brain phospholipids in aging and in Alzheimer's disease. Lipids 26:421-425. Sohrabji F, Miranda RC, Toran-Allerand CD (1995) Identification of a putative estrogen response element in the gene encoding brain-derived neurotrophic factor. Proc Natl Acad Sci U S A 92:11110-11114. Su HM (2010) Mechanisms of n-3 fatty acid-mediated development and maintenance of learning memory performance. J Nutr Biochem 21:364-373. Takeuchi T, Fukumoto Y, Harada E (2002) Influence of a dietary n-3 fatty acid deficiency on the cerebral catecholamine contents, EEG and learning ability in rat. Behav Brain Res 131:193-203. Takeuchi T, Iwanaga M, Harada E (2003) Possible regulatory mechanism of DHA-induced anti-stress reaction in rats. Brain Res 964:136-143. Tarttelin MF, Gorski RA (1973) The effects of ovarian steroids on food and water intake and body weight in the female rat. Acta Endocrinol (Copenh) 72:551-568. Tsien JZ, Huerta PT, Tonegawa S (1996) The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory. Cell 87:1327-1338. Tully AM, Roche HM, Doyle R, Fallon C, Bruce I, Lawlor B, Coakley D, Gibney MJ (2003) Low serum cholesteryl ester-docosahexaenoic acid levels in Alzheimer's disease: a case-control study. Br J Nutr 89:483-489. Walf AA, Frye CA (2005) Antianxiety and antidepressive behavior produced by physiological estradiol regimen may be modulated by hypothalamic-pituitary-adrenal axis activity. Neuropsychopharmacology 30:1288-1301. Walf AA, Frye CA (2006) A review and update of mechanisms of estrogen in the hippocampus and amygdala for anxiety and depression behavior. Neuropsychopharmacology 31:1097-1111. Warren SG, Juraska JM (1997) Spatial and nonspatial learning across the rat estrous cycle. Behav Neurosci 111:259-266. Wise PM, Dubal DB, Wilson ME, Rau SW, Liu Y (2001) Estrogens: trophic and protective factors in the adult brain. Front Neuroendocrinol 22:33-66. Woolley CS, McEwen BS (1993) Roles of estradiol and progesterone in regulation of hippocampal dendritic spine density during the estrous cycle in the rat. J Comp Neurol 336:293-306. Woolley CS, McEwen BS (1994) Estradiol regulates hippocampal dendritic spine density via an N-methyl-D-aspartate receptor-dependent mechanism. J Neurosci 14:7680-7687. Woolley CS, Gould E, Frankfurt M, McEwen BS (1990) Naturally occurring fluctuation in dendritic spine density on adult hippocampal pyramidal neurons. J Neurosci 10:4035-4039. Wu A, Ying Z, Gomez-Pinilla F (2004) Dietary omega-3 fatty acids normalize BDNF levels, reduce oxidative damage, and counteract learning disability after traumatic brain injury in rats. J Neurotrauma 21:1457-1467. Wu A, Ying Z, Gomez-Pinilla F (2008) Docosahexaenoic acid dietary supplementation enhances the effects of exercise on synaptic plasticity and cognition. Neuroscience 155:751-759. Zandi PP, Carlson MC, Plassman BL, Welsh-Bohmer KA, Mayer LS, Steffens DC, Breitner JC (2002) Hormone replacement therapy and incidence of Alzheimer disease in older women: the Cache County Study. JAMA 288:2123-2129 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23425 | - |
dc.description.abstract | 性荷爾蒙雌激素(estrogen)與必需脂肪酸二十二碳六烯酸(docosahexaenoic acid, DHA, 22:6n-3)均為影響腦部神經發育的重要因子,如保護神經與增加突觸可塑性。臨床研究發現停經後婦女接受雌激素補充療法後,血漿中的DHA濃度也隨之增加,並能降低阿茲海默症罹患率。本研究主要探討雌激素與DHA兩者對學習記憶與腦區的突觸蛋白表現是否有交互作用。
研究方法使用Sprague-Dawley大鼠,終生食用缺乏n-3 多元不飽和脂肪酸(polyunsaturated fatty acid, PUFA)飼料。雌鼠鼠齡為2個月性成熟後進行卵巢切除手術(ovariectomy)以剝奪其體內雌激素,卵巢切除後5個月,即鼠齡7個月開始補充魚油和/或雌激素,同時記錄體重與食量變化,於鼠齡9個月進行十字迷宮、強迫游泳測試、主動迴避測試與Morris水迷宮行為測試。在鼠齡12個月進行犧牲,收集腦部與周邊組織,進行腦部突觸蛋白質定量分析,包括:BDNF、NMDA受體(NR2A與NR2B)、synapsin I、及CaMKIIα。 結果如下:(1)單獨補充雌激素或合併補充魚油,能觀察到體重與總脂肪重的下降,以及子宮、腦下垂體與下視丘的重量上升。(2)單獨補充雌激素能促進海馬迴與前額葉皮質的synapsin I蛋白表現,單獨補充魚油也能促進海馬迴的synapsin I蛋白表現,合併補充雌激素與魚油則無觀察到協同作用。(3)單獨補充魚油及併用雌激素均能促進前額葉皮質的CaMKIIα蛋白表現,單獨補充雌激素則無作用。(4)合併補充魚油與雌激素能促進前額葉皮質的NR2A蛋白表現。(5)單獨或同時補充魚油與雌激素均無法改善個體的焦慮行為。(6)單獨補充魚油可提升主動迴避學習行為。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-08T05:01:27Z (GMT). No. of bitstreams: 1 ntu-99-R97441013-1.pdf: 1790734 bytes, checksum: 35b7a76e1e787c7a9cce2bc19ccdd0c5 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
中文摘要 I 英文摘要 II 目錄 III 圖表目錄 VIII 第一章 文獻回顧 1 一、引言 1 二、Docosahexaenoic acid (22:6 n-3,DHA) 1 三、雌激素 3 四、突觸可塑性 4 4-1 Brain-derived neurotrophic factor (BDNF) 5 4-2 N-methyl-D-aspartate receptor (NMDA receptor) 6 4-3 Calmodulin-dependent kinases II (CaMKII) 6 4-4 Synapsin I 7 五、雌激素、二十二碳六烯酸與學習行為 7 第二章 研究目的 9 一、研究動機 9 二、研究假說與架構 9 三、研究重要性 10 第三章 材料與方法 11 一、實驗動物與飼養條件 11 二、實驗飼料組成 11 三、動物分組與實驗流程 12 3-1 動物分組 12 3-2 實驗流程 12 四、兩側卵巢切除手術 13 五、行為實驗 14 5-1 焦慮行為測試 14 5-2 憂慮行為測試 14 5-3 主動迴避學習能力測試 15 5-4 空間學習記憶能力測試 15 六、動物犧牲 16 七、腦部突觸蛋白表現 17 7-1 蛋白質萃取 17 7-2. 蛋白質定量分析 18 7-3. 電泳檢定法 18 7-4. 蛋白質轉印 21 7-5. 阻隔 22 7-6. 酵素免疫染色法 23 7-7. 曝光 24 八、統計分析 24 第四章 結果 25 一、食量與體重變化 25 二、犧牲概況 26 2-1 子宮重量與總脂肪重量 26 2-2 腦下垂體重量、下視丘重量與其他 28 三、焦慮行為 29 四、憂慮行為 31 五、主動迴避試驗 32 六、空間學習能力 33 七、突觸蛋白表現 34 7-1. BDNF 34 7-2 NMDA receptor:NR2A與NR2B 35 7-3 CaMKIIα 36 7-4 Synapsin I 37 第五章 討論 38 一、卵巢切除與補充雌激素對食量、體重與血清雌激素濃度的影響 38 二、卵巢切除與補充雌激素和/或魚油後對個體行為的影響 38 2-1 焦慮行為 38 2-2 憂慮行為 39 2-3 主動迴避學習 39 2-4 空間學習記憶 40 三、卵巢切除與補充雌激素和/或魚油後對腦部突觸蛋白表現的影響 40 3-1 BDNF 41 3-2 NMDA receptor 41 3-3 CaMKIIα 42 3-4 synapsin I 43 第六章 總結 44 第七章 參考文獻 45 | |
dc.language.iso | zh-TW | |
dc.title | 探討大鼠去卵巢後給予雌激素與魚油補充對海馬迴突觸蛋白表現及學習行為之影響 | zh_TW |
dc.title | Effects of estrogen and fish oil supplementation on hippocampal synaptic protein expression and learning memory performance studies in ovariectomized rats | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡元奮,盧國賢,黃青真 | |
dc.subject.keyword | 雌激素,魚油,突觸蛋白, | zh_TW |
dc.subject.keyword | estrogen,fish oil,synaptic protein, | en |
dc.relation.page | 50 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2010-12-06 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 生理學研究所 | zh_TW |
顯示於系所單位: | 生理學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 1.75 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。