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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳敏慧(Min-Huey Chen) | |
| dc.contributor.author | Yi-Wen Han | en |
| dc.contributor.author | 韓依彣 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:49:03Z | - |
| dc.date.copyright | 2009-09-15 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-28 | |
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Transcript profiling of periodontal fibroblasts and osteoblasts. Lallier TE, Spencer A, Fowler MM (2005). J Periodontol. 76(7):1044-55. 67. TsukamotoY, Fukutani S, Shin-Ike T, Kubota T, Sato S, Suzuki Y, Mori M (1992). Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts. Arch Oral Biol. Dec;37(12) : 1045-55. 68. Tziafas D (1995). Basic mechanisms of cytodifferentiation and dentinogenesis during dental pulp repair. Int J Dev Biol 39:281–290 69. van der Burgt TP and Plasschaert AJ (1985). Tooth discoloration induced by dental materials. Oral Surg Oral Med Oral Pathol 60, 666–669. 70. van der Burgt TP, Mullaney TP and Plasschaert AJ (1986). Tooth discoloration induced by endodontic sealers. Oral Surg Oral Med Oral Pathol 61, 84–89. 71. von der Fehr, FR, Loe H, Theilade E (1970). Experimentalcaries in man. Caries Res. 4, 131-148. 72. Wei X, Ling J, Wu L, Liu L, Xiao Y (2007). Expression of mineralization markers in dental pulp cells. J Endod 33(6):703-8. 73. Wurtz T, Houari S, Mauro N, MacDougall M, Peters H, Berdal A (2008). Fluoride at non-toxic dose affects odontoblast gene expression in vitro.Toxicology 10;249(1):26-34. 74. Xiao S, Yu C, Chou X, Yuan W, Wang Y, Bu L, et al. (2001). Dentinogenesis imperfecta 1 with or without progressive hearing loss is associated with distinct mutations in DSPP. Nat Genet 27:201–204. 75. Xu T, Bianco P, Fisher LW, Longenecker G, Smith E, Goldstein S, et al. (1998). Targeted disruption of the biglycan gene leads to an osteoporosis-like phenotype in mice. Nat Genet 20:78–82. 76. Yamamura T (1985). Differentiation of pulpal cells and inductive influences of various matrices with reference to pulpal wound healing. J Dent Res 64: 530–540. 77. Yamauchi M, Chandler GS and Katz EP (1992). Collagen cross.linking and mineralization. In Chemistry and Biology of Mineralized Tissues (Eds H. Slavkin and P.A. Price). Elsevier, New York, 39-46. 78. Zhang W, Walboomers XF, Wolke JG, Bian Z, Fan MW and Jansen JA (2005). Differentiation ability of rat postnatal dental pulp cells in vitro. Tissue Eng 11 79. Zhang X, Zhao J, Li C, Gao S, Qiu C, Liu P, et al. (2001). DSPP mutation in dentinogenesis imperfecta Shields type II. Nat Genet 27:151–152. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23236 | - |
| dc.description.abstract | 氟素在牙科公共衛生上的應用已經持續很長的一段時間。氟對於預防孩童齲齒的發生是很重要，也是很有效的一個藥物。另一方面因為氟可以刺激骨母細胞的增生以及刺激骨組織的形成，氟素也被拿來當作治療骨質疏鬆症。在即將脫落的乳牙牙髓組織中，存在多能力的幹細胞。而這些幹細胞具有高度增生及分化能力的特性，並且可分化成為牙本質母細胞。然而氟對於乳牙牙髓細胞作用的毒性或是其作用的角色都尚未清楚。所以本實驗的主要目的是觀察氟化鈉對於體外培養乳牙牙髓細胞其生長能力和礦化基因表現的影響 。
乳牙牙髓細胞是以2.5x104cell/cm2的密度培養於含氟化鈉濃度為0, 10-6, 10-5, 10-4, 5x10-4及10-3M之培養液來進行MTT測試觀察細胞生長的情形。另外將乳牙牙髓細胞同樣以密度培養於0, 10-6, 10-5, 10-4 及 5x10-4M並和培養於牙本質細胞誘導礦化培養液中的細胞進行鹼性磷酸酶(alkaline phosphatase)、鈣化能力以及礦化基因表現的比較。 在MTT測試中，發現到當氟化鈉的濃度為10-3M，乳牙牙髓細胞在培養的第1天以及第3天其細胞生長的活性是受到限制。然而在培養的第10天，在含氟化鈉培養基的細胞，其生長活性高於控制組。在鹼性磷酸酶活性試驗中，在培養的第5天和第7天，含氟化鈉培養基的細胞，其活性都低於控制組；然而到了第14天，有接受氟化鈉刺激的細胞，其活性超越控制組。在礦化基因表現，在培養第7天和第10天，鹼性磷酸酶基因的表現低於控制組和牙本質細胞誘導礦化組；於培養第14天，鹼性磷酸酶基因的表現則會高於控制組。在骨鈣蛋白基因表現，於培養的第7天，於含氟化鈉培養基中的細胞其表現較弱；然而第14天含有氟化鈉培養基中的細胞基因的表現則高於控制組。由以上的結果可以推論，氟化鈉確實會影響乳牙牙髓細胞的生長以及礦化表現，氟化鈉對於調控乳牙牙髓細胞生長及礦化基因的表現仍需做進一步的研究。 | zh_TW |
| dc.description.abstract | The use of fluorides in dental public health programs has a long history. Fluoride is an important and effective factor for reducing the caries incidence in children. Fluoride also increases proliferation of osteoblasts and stimulates bone formation. Exfoliated human deciduous tooth contains multipotent stem cells in the pulp tisuue. These cells were identified to be a population of highly proliferative, clonogenic cells capable of differentiating into odontoblasts. However the cellular and molecular pathways of fluoride toxicity in pulp cells derived exfoliated deciduous teeth are not very well understood. The objective of the present study was to evaluate the effects of sodium fluoride (NaF) on pulp cells derived from exfoliated deciduous teeth cultured in vitro.
Pulp cells at 2.5x104cell/cm2 were incubated in vitro with NaF at 0, 10-6, 10-5, 10-4, 5x10-4 and 10-3M and MTT test was examined. Pulp cells at 2.5x104cells/cm2 in density were also incubated in vitro with NaF at 0, 10-6, 10-5, 10-4 and 5x10-4M compared to odontogenic induction medium, and then the ALP activity, calcification and mineralization were examined. A cell based quantitative evaluation of the MTT assay showed that NaF at concentration of 10-3M arrested cell growth on day 1 and day 3. Whereas the MTT assay showed that higher proliferative rate treated with NaF versus control on day 10. Alkaline phosphatase activity decreased in cells treated with NaF on day 5 and day 7 versus control. On day 14, cells treated with NaF had higher alkaline phosphatase activity than control. The gene of alkaline phosphatase expression in cells treated with NaF decreased on day 7 and day 10 versus control and induction group but enhanced on day 14. Osteocalcin expression of NaF treated cells decreased on day 7 versus control but enhanced on day 14. In conclusion, results of this study indicated that NaF modulates proliferation and mineralization pulp cells derived from exfoliated deciduous teeth proliferation and mineralization. This findings suggest that NaF may play a significant role in pulp cells physiology. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:49:03Z (GMT). No. of bitstreams: 1 ntu-98-R95422006-1.pdf: 56273007 bytes, checksum: c81684ce780f5e03dc07ba95a211c4f4 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 中文摘要 V
Abstract VII 第一章 引言 1 第二章 文獻回顧 2 第一節 牙髓組織 2 1.1 牙髓組織的組成及功能 2 1.2 牙髓再生 3 1.3 牙髓幹細胞 4 第二節 氟化物 5 2.1 氟在牙科的應用 5 2.2 氟對其他細胞的影響 7 第三節 細胞分化和成骨指標蛋白基因的表現 8 3.1 鹼性磷酸酶（Alkaline phosphatase，ALP） 9 3.2 骨鈣蛋白（Osteocalcin，OCN） 10 3.3 牙本質涎磷蛋白（Dentin sialophosphoprotein，DSPP） 11 第三章 實驗目的 13 第四章 研究材料與方法 14 第一節 所使用的細胞及細胞培養條件 14 1.1 人類乳牙牙髓組織的取得 14 1.2 培養牙髓細胞之先關藥品配製 14 1.3 乳牙牙髓細胞之培養 15 第二節 氟化鈉（So d i um F l uo ri de , Na F）對於乳牙牙髓細胞生長能力實驗 15 2.1 含氟化鈉細胞培養液之配製 15 2.2 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide)測試 15 第三節 氟化鈉（So d i um F l uo ri de , Na F）對於乳牙牙髓細胞誘導分化實驗 16 3.1 牙本質母細胞誘導分化培養液之配製 16 3.2 鹼性磷酸酶 （Alkaline Phosphatase , ALP）活性 16 3.3 Alizarin Red Stain 18 3.5 反轉錄聚合酶連鎖反應 (Reverse Transcription-Polymerase Chain Reaction，RTPCR 19 第四節 統計分析 22 第五章 實驗結果 23 第一節 人類乳牙牙髓細胞之培養 23 1.1 人類乳牙牙髓細胞進行流式細胞儀分析 （Flow cytometry） 23 第二節 氟化鈉對乳牙牙髓細胞生長能力試驗 23 第三節 氟化鈉對乳牙牙髓細胞礦化能力試驗 23 3.1 鹼性磷酸酶染色之結果 23 3.2 鹼性磷酸酶活性定量試驗結果 24 3.3 Alizarin red stain 結果 25 3.4 反轉錄聚合酶連鎖反應 (Reverse Transcription-Polymerase Chain Reaction，RTPCR 25 3.5 即時定量聚合酶連鎖反應(Real-time PCR). 26 第六章 討論 28 第一節 人類乳牙牙髓細胞的培養 2 8 第二節 人類乳牙牙髓細胞之間質幹細胞表面標幟 2 8 第三節 氟化鈉對乳牙牙髓細胞生長的影響 2 9 第四節 鹼性磷酸酶活性測試和鹼性磷酸酶染色試驗 3 1 第五節 A l iz a r i n R e d St a i n 3 2 第六節 反轉錄聚合酶連鎖反應和即時定量聚合酶連鎖反應 3 4 6.1 鹼性磷酸酶（Alkaline phosphatase, ALP） 35 6.2 骨鈣蛋白（Osteocalcin, OCN） 35 6.3 第一型膠原蛋白（Collagen Type I，COL type I） 36 6.4 牙本質涎磷蛋白（dentin sialophosphoprotein，DSPP） 36 第七章 結論 38 第八章 未來研究方向 39 參考文獻 40 | |
| dc.language.iso | zh-TW | |
| dc.subject | 牙髓細胞 | zh_TW |
| dc.subject | 氟化鈉 | zh_TW |
| dc.subject | 乳牙 | zh_TW |
| dc.subject | 礦化 | zh_TW |
| dc.subject | 牙本質分化 | zh_TW |
| dc.subject | odontogenic differentiation | en |
| dc.subject | Sodium fluoride | en |
| dc.subject | pulp cells | en |
| dc.subject | exfoliated deciduous teeth | en |
| dc.subject | mineralization | en |
| dc.title | 氟化鈉對乳牙牙髓細胞生長以及礦化基因表現的調控 | zh_TW |
| dc.title | Regulation of growth and mineralizing genes expression by sodium fluoride in pulp cells from human exfoliated deciduous teeth | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 郭敏光 | |
| dc.contributor.oralexamcommittee | 林俊彬,楊台鴻 | |
| dc.subject.keyword | 氟化鈉,牙髓細胞,乳牙,礦化,牙本質分化, | zh_TW |
| dc.subject.keyword | Sodium fluoride,pulp cells,exfoliated deciduous teeth,mineralization,odontogenic differentiation, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2009-07-28 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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