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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝文陽 | |
| dc.contributor.author | Yin-Ting Chen | en |
| dc.contributor.author | 陳盈廷 | zh_TW |
| dc.date.accessioned | 2021-06-08T04:37:27Z | - |
| dc.date.copyright | 2009-08-18 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-17 | |
| dc.identifier.citation | Aguilera, M., Jimenez-Pranteda, M. L., Kharroub, K., Gonzalez-Paredes, A., Durban, J. J., Russell, N. J., Ramos-Cormenzana, A. & Monteoliva-Sanchez, M. (2009). Marinobacter lacisalsi sp. nov. a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve 'Fuente de Piedra' in Southern Spain. International Journal of Systematic and Evolutionary Microbiology 59, 1691-1695.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23006 | - |
| dc.description.abstract | 碳氫化合物為石油的主要成份之一。本實驗室於2008年分離出二十九株能夠以十六烷作為單一碳源生長的分離菌，其中六株菌株經半年保存培養後失去其分解活性。將分解效率最高之四菌株作為研究對象，鑑種並利用氣相層析儀探討它們在本實驗室之人工海水、含有機營養鹽的人工海水與過濾海水間降解十六烷之差異。經16S rDNA序列分析與生理生化特性測試，判定四菌株HD5、HD8、HD51、MO27分別為Marinobacter hydrocarbonoclasticus、Alcanivorax dieselolei、Marinobacter mobilis、Alcanivorax balearicus，並發現過濾海水相較於人工海含有能夠促進菌株適應新系統的成份。含量較高的氮鹽、磷鹽等營養鹽或有機營養物質能助長菌株HD5和菌株MO27對十六烷的利用；同屬之HD8、MO27二菌株擁有不同基因序列與alkB基因數量，且其對三種系統環境的反應也不同。 | zh_TW |
| dc.description.abstract | The main composition of petroleum oil is hydrocarbon. Our laboratory isolated 29 bacterial strains which were able to utilize hexadecane as sole carbon source in 2008. After conserving strain sources for six months, six strains lost hydrocarbon-degrading ability. Four of the fastest growing bacterial strains were selected as material to study their hexadecane-utilization efficiency in three culture conditions: artificial seawater, artificial seawater with organic nutrient and filtered natural seawater. Strain HD5, HD8, HD51 and MO27 were identified as Marinobacter hydrocarbonoclasticus, Alcanivorax dieselolei, Marinobacter mobilis and Alcanivorax balearicus. Bacterial cells could adapt to new system more quickly in filtered natural seawater than in artificial seawater. Higher nitrogenous and phosphoric nutrients or organic nutrient content can improve hexadecane utilization by isolates HD5 and MO27. HD8 and MO27, classified as the same genus, had different gene sequences and number of alkB genes, and had different reactions to three culture conditions. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T04:37:27Z (GMT). No. of bitstreams: 1 ntu-98-R96241214-1.pdf: 1168987 bytes, checksum: b5f9827364c2537012a263181df24549 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 目錄 iii
表目錄 iv 圖目錄 v 中文摘要 vi Abstract vii 第一章 前言 1 一、石油於人類的益處與害處 1 二、石油汙染物的處理 1 三、降解石油的微生物 2 四、影響石油生物降解的因素 3 五、研究動機與目的 4 第二章 材料與方法 5 一、培養基配置 5 二、細菌樣本 5 三、分解效率再現性(reproducibility)之測試 5 四、菌株鑑定 6 五、十六烷分解效率 13 第三章 結果與討論 16 一、分解效率再現性之測試 16 二、16S rDNA序列分析與生理生化特性比較 17 三、菌株於PY培養基之生長狀況 19 四、菌株於三種培養液培養96小時後之十六烷降解結果 20 五、菌株以十六烷作為單一碳源之生長曲線與十六烷降解量變化 21 六、菌株的利用與未來展望 24 第四章 總結 25 參考文獻 26 附錄 51 | |
| dc.language.iso | zh-TW | |
| dc.title | 臺灣沿岸細菌分離株降解十六烷之研究探討 | zh_TW |
| dc.title | Comparison of hexadecane utilization by bacteria isolates from coastal water of Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 簡文達,蔡珊珊,李重義 | |
| dc.subject.keyword | 十六烷降解,培養條件,氣相層析儀,Marinobacter,Alcanivorax, | zh_TW |
| dc.subject.keyword | hexadecane degradation,culture condition,gas chromatography,Marinobacter,Alcanivorax, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2009-08-17 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| 顯示於系所單位： | 海洋研究所 | |
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