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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 張繼堯 | |
dc.contributor.author | Guo-Wen Pan | en |
dc.contributor.author | 潘國文 | zh_TW |
dc.date.accessioned | 2021-06-08T04:37:14Z | - |
dc.date.copyright | 2009-08-20 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-17 | |
dc.identifier.citation | 蘇惠美,1999。餌料生物之培養與利用。台灣省水產試驗所東港分所。
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Oostende, Belgium, European Aquaculture Society, Special Publication No. 30. Kahan, D., Berman, Y. & Bar-El, T. 1988. Maternal inhibition of hatching at high population densities in Tigriopus japonicus (Copepoda, Crustacea). Biological Bulletin 174:139-144. Kim I. C., Kim Y. J., Lee Y. M., Kim B. G., Park T. J., et al. 2004. cDNA Cloning of Translationally Controlled Tumor Protein/Histamine Releasing Factor (TCTP/HRF) from the Intertidal Harpacticoid Copepod Tigriopus japonicas. DNA seq. 15 (2), 159-163. Lee, C. & Hu, F. 1981. Salinity tolerance and salinity affects on brood size of Tigriopus japonicus Mori. Aquaculture 22:377-381. Lee, W.H., Zhang, X.K., Van Baalen, C. & Arnold, C.R. 1985. Feeding and reproductive performance of the harpacticoid Tisbe carolinensis (Copepoda, Crustacea) in four algal cultures. Marine Ecology Progress Series 24:273-279. Lee C. S., O’Bryen P. J., Marcus N. H.2005. Copepods in Aquaculture. Blackwell. Miliou, H. & Moraitou-Apostolopoulou, M. 1991. Combined effects of temperature and salinity on the population dynamics of Tisbe holothuriae Humes (Copepoda, Harpacticoida). Archiv fur Hydrobiologie 121:431-448. Miliou, H. & Moraitou-Apostolopoulou, M. 1991. Effects of seven diets on the population dynamics of laboratory cultured Tisbe holothuriae Humes (Copepoda, Harpacticoida). Helgolander Meeresuntersuchungen 45:345-356. Marcus, N.H., Lutz, R., Burnett, W. & Cable, P. 1994. Age, viability, and vertical distribution of zooplankton resting eggs from an anoxic basin: evidence of an egg bank. Limnology and Oceanography 39:154-158. Navarro, J.C., Batty, R.S., Bell, M.V. & Sargent, J.R. 1993. Effects of two Artemia diets with different contents of polyunsaturated fatty acids on the lipid composition of larvae of Atlantic herring (Clupea harengus). J. Fish Biol. 43:503-515. Naess, T., Germain-Henry, M. & Naas, K.E. 1995. First feeding of Atlantic halibut (Hippoglossus hippoglossus) using different combinations of Artemia and wild zooplankton. Aquaculture 130:235-250. Nanton, D. A. and Castell, J. D. 1998. The effects of dietary fatty acids on the fatty acid composition of the harpacticoid copepod, Tisbe sp., for use as a live food for marine fish larvae. Aquaculture 163, 251-261. Sargent, J.R. & Henderson, R.J. 1986. Lipids. In: The Biological Chemistry of Marine Copepods (Corner, E.D.S. & O’Hara, S.C.M., eds), pp.59-108. Oxford University Press, New York, NY. Santella, L. & Ianora, A. 1990. Subitaneous and diapause eggs in Mediterranean populations of Pontella mediterranea (Copepoda: Calanoida): a morphological study. Marine Biology 105:83-90. Segner, H., Storch, V., Reinecke, M., Kloas, W. & Hanke, W. 1994. The development of functional digestive and metabolic organs in turbot, Scophthalmus maximus. Mar. Biol. 119:471-486. Sun, B. & Fleeger, J.W. 1995. Sustained mass culture of Amphiascoides atopus a marine harpacticoid copepod in a recirculating system. Aquaculture 136:313-321. Su, H. M., M. S. Su, and I. C. Liao, 1997. Collection and culture of live foods for aquaculture in Taiwan. Hydrobiology, 358: 37-40. Sargent, J.R., McEvoy, L.A. & Bell, J.G. 1997. Requirements, presentation and sources of polyunsaturated fatty acids in marine fish larval feeds. Aquaculture 155:117-127. Stottrup, J.G. & Norsker, N.H. 1997. Production and use of copepods in marine fish larviculture.Aquaculture 155:231-247. Stottrup, J.G. & Norsker, N.H. 1997. Production and use of copepods in marine fish larviculture. Aquaculture 155:231-247. Shields R. J., Bell J. G., Luizi F. S., Gara B., Bromage N. R. 1999. Natural Copepods Are Superior to Enriched Artemia Nauplii as Feed for Halibut Larvae (Hippoglossus hippoglossus) in Terms of Survival, Pigmentation and Retinal Morphology: Relation to Dietary Essential Fatty Acids J. Nutr. 129(6), 1186-94. Sargent, J., Bell, G., McEvoy, L., Tocher, D. & Esteves, A. 1999. Recent developments in the essential fatty acid nutrition of fish. Aquaculture 177:191-199. Takano, H. 1971. Breeding experiments of a marine littoral copepod Tigriopus japonicus Mori. Bulletin of the Tokai Regional Fisheries Research Laboratory 64:71-80. Watanabe, T., Kitajima, C. & Fujita, S. 1983. Nutritional values of live organisms used in Japan for mass propagation of fish: a review. Aquaculture 34:115-143. Zhang, Q. & Uhlig, G. 1993. Effects of density on larval development and female productivity of Tisbe holothuriae (Copepoda, Haepacticoida) under laboratory conditions. Helgolander Meeresuntersunchungen 47:229-241. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23001 | - |
dc.description.abstract | 橈足類也許是地球上數量最多的多細胞生物,超過11,500個種類比陸地上的昆蟲數量還多。此成功演化的原因主要歸功於其高生殖率及快速的胚胎發生。而海洋橈足類也成為自然界孕育魚苗最佳的餌料生物。橈足類因為體型小富含高度長鏈不飽和脂肪酸,有潛力發展成為魚苗初期發育最佳的餌料生物。本研究對象日本虎斑猛水蚤對環境適應力強,且為雜食性,容易培養,因此有潛力發展為大量培養的魚苗生物餌料種類之一。關於日本虎斑猛水蚤生長發育及營養分析的研究不少,但基因層次上的相關研究並不多。本研究利用餵食日本虎斑猛水蚤不同的食物,並探討其生長情形與體內脂肪酸組成,再構築日本虎斑猛水蚤之cDNA基因庫,來探討其營養代謝合成相關基因和發育生長相關基因群之表現。本實驗以不同食物來培養日本虎斑猛水蚤14天,並觀察族群變化。於第14天收集蟲體分析脂肪酸組成,發現餵食周氏扁藻的蟲體,密度為每公升302.5隻,DHA和EPA分別佔總脂肪酸的1.56%與1.58%。餵食發酵液的蟲體,密度為每公升390隻,DHA和EPA分別佔總脂肪酸的5.53%與8.27%。餵食酵母的蟲體,密度為每公升392.5隻,DHA和EPA分別佔總脂肪酸的7.57%與11.57%。餵食奶粉的蟲體,密度為每公升1,217.5隻,DHA和EPA分別佔總脂肪酸4.24%與6.12%。以產量和食物取得便利性而言,奶粉為培養日本虎斑猛水蚤之最佳食物來源。本實驗利用cDNA 微陣列晶片經交叉比對後篩選出142個差異性表現基因,經定序比對分析,其中86個比對到相似序列基因,11個為功能不明的基因序列,扣除重複後共得72個促進表現基因。這些促進表現基因包括轉譯因子、呼吸鏈、結構、信息傳遞、發育、代謝、生長因子、運輸蛋白和調控蛋白等。此外,我們也選殖出Delta-9-desaturase基因,此將有助於日本虎斑猛水蚤在脂質代謝方面的相關研究。 | zh_TW |
dc.description.abstract | Copepods may be the most abundant multi-cellular organism in the world, which contains more than 11,500 species that more than that of insects. Its evolutionary success contributes to its high reproduction rate and rapid embryonic development, and the oceanic copepods naturally become the best living feed for fish fry in the nature. Copepods has the potential to become the best living feed for fish fry early development because of its small size and rich in long chain unsaturated fatty acid. The Tigriopus japonicus in this study has great adaptability to environment, polyphagia and easy to culture, so has the potential to develop as one of the fish fry living feed species that can be mass produced. Although there are quite a few studies on growth development and nutritional analysis of Tigriopus japonicus, however, the study on genetic level is still quite limited. In this study, we fed Tigriopus japonicus with different food to investigate the growth situation and fatty acid composition. The cDNA library of Tigriopus japonicus was further constructed to discuss the expression of nutritional metabolism related genes and development related genes. Tigriopus japonicus were fed with different food for 14 days and the population changes were observed. The fatty acid composition analysis on the 14th day showed that the density of Tigriopus japonicus fed with Tetraselmis chui was 302.5/L, and the DHA and EPA was 1.56% and 1.58% of total fatty acid respectively. The density of Tigriopus japonicus fed with fermented liquid was 390/L, and the DHA and EPA was 5.53% and 8.27% of total fatty acid respectively. The density of Tigriopus japonicus fed with yeast was 392.5/L, and the DHA and EPA was 7.57% and 11.57% of total fatty acid respectively. The density of Tigriopus japonicus fed with milk powder was 1217.5/L, and the DHA and EPA was 4.24% and 6.12% of total fatty acid respectively. From the production amount and convenience point of view, milk powder is the best food source of Tigriopus japonicus culture. In this study, we screened and obtained 142 up-regulated genes from cDNA library. After blasting, 86 of which can find the homologous genes, 11 of which are unknown function. The up-regulated genes include transcription factors, respiratory chain, structure, signal transduction, development, metabolism, growth factors, transportation proteins and regulatory proteins. Besides, we also subcloned Delta-9-desaturasae gene, which might be helpful on the research of Tigriopus japonicus on lipid metabolism. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:37:14Z (GMT). No. of bitstreams: 1 ntu-98-R96b45017-1.pdf: 2537668 bytes, checksum: 9e6a89af610cb65250cff3cb595f3df5 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 目 錄
中文摘要 Ⅰ 英文摘要 Ⅱ 目錄 Ⅳ 圖目次 Ⅶ 表目次 Ⅷ 壹、前言 1 1.1 台灣石斑魚簡介與養殖現況 1 1.2 石斑魚魚苗之餌料生物 2 1.3 橈足類之生活史(以猛水蚤目為例) 4 1.4 不同食物對橈足類營養品質和族群生長的影響 5 1.5 橈足類在養殖業上的運用 5 1.6 日本虎斑猛水蚤(Tigriopus japonicus)之簡介 6 1.7 周氏扁藻 (Tetraselmis chui)之簡介 7 1.8 實驗目的 8 貳、材料與方法 9 2.1 生物性材料 9 2.1.1 日本虎斑猛水蚤 9 2.1.2 周氏扁藻 9 2.2 不同食物條件對日本虎斑猛水蚤族群生長的影響 9 2.2.1 藥品 9 2.2.2 周氏扁藻的培養 10 2.2.3 日本虎斑猛水蚤培養 11 2.3 不同食物與日本虎斑猛水蚤的脂肪酸分析 12 2.3.1 藥品 12 2.3.2 粗脂肪萃取 13 2.3.3 皂化 13 2.3.4 甲基酯化 14 2.3.5 氣相層析 (Gas Chromatograph,GC) 14 2.4 日本虎斑猛水蚤cDNA基因庫之構築 15 2.4.1 藥品 15 2.4.2 日本虎斑猛水蚤total RNA之萃取 18 2.4.3 日本虎斑猛水蚤 mRNA之純化與RNA電泳 18 2.4.4 第一股cDNA合成 19 2.4.5 第二股cDNA合成 19 2.4.6 Complementary DNA兩端的修飾 20 2.4.7 第一股及第二股cDNA分析 20 2.4.8 EcoRⅠ adaptor的連接及磷酸化作用 20 2.4.9 XhoⅠ切割作用 21 2.4.10 分離不同大小之cDNA片段 21 2.4.11 Complementary DNA嵌入片段與載體之接合反應 21 2.4.12 λ噬菌體之包裝反應 22 2.4.13 日本虎斑猛水蚤cDNA基因庫效價之測定 22 2.4.14 Mass excision 22 2.5 Complementary DNA 微陣列之雜合反應 23 2.5.1 藥品 23 2.5.2 日本虎斑猛水蚤樣品探針之製備 24 2.5.3 Total RNA 之萃取、純化與RNA電泳 24 2.5.4 基因微陣列晶片之前準備 25 2.5.5 Complementary DNA合成與標定 25 2.5.6 Complementary DNA微陣列晶片之雜合反應 27 2.5.7 候選基因定序及序列比對分析 27 參、結果 28 3.1 不同食物來源對日本虎斑猛水蚤族群生長的影響 28 3.2 不同食物與虎斑猛水蚤的脂肪酸分析 28 3.2.1 不同食物之脂肪酸組成分析 28 3.2.2 以不同食物餵養的日本虎斑猛水蚤之脂肪酸組成分析 29 3.3 日本虎斑猛水蚤λ 噬菌體cDNA 基因庫之建立與力價之測定 29 3.4 Complementary DNA微陣列之雜合反應 30 3.5 Complementary DNA微陣列數據之分析 30 3.6 差異表現 cDNA之序列分析 31 肆、討論 32 伍、參考文獻 36 圖目次 圖一、 以不同食物餵食日本虎斑猛水蚤之生長曲線 42 圖二、 不同食物脂肪酸組成的氣相層析 43 圖三、 以不同食物餵食的日本虎斑猛水蚤脂肪酸組成的氣相層析 44 圖四、 日本虎斑猛水蚤mRNA之萃取 45 圖五、 雙股cDNA合成 46 圖六、 利用CL-2B管柱層析分離雙股cDNA 47 圖七、 不同食物對應於發酵液培養日本虎斑猛水蚤之基因表現微陣列雜合 48 圖八、 不同食物對應於酵母培養日本虎斑猛水蚤之基因表現微陣列雜合 49 圖九、 不同食物對應於奶粉培養日本虎斑猛水蚤之基因表現微陣列雜合 50 圖十、 不同食物對應於發酵液培養日本虎斑猛水蚤基因表現大於1.5倍之偉恩圖 51 圖十一、 不同食物對應於酵母培養日本虎斑猛水蚤基因表現大於1.5倍之偉恩圖 52 圖十二、 不同食物對應於奶粉培養日本虎斑猛水蚤基因表現大於1.5倍之偉恩圖 53 表目次 表一、 不同食物之脂肪酸組成分析 54 表二、 以不同食物培養的日本虎斑猛水蚤之脂肪酸組成分析 55 表三、 虎斑猛水蚤cDNA基因庫力價之測定 56 表四、 餵食不同食物之日本虎斑猛水蚤的促進表現基因 57 | |
dc.language.iso | zh-TW | |
dc.title | 日本虎斑猛水蚤之基因表現與功能性基因庫篩選 | zh_TW |
dc.title | Gene expression and function genomic library screening of copepod Tigriopus japonicus | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇建國,林正輝 | |
dc.subject.keyword | 虎斑猛水蚤,不飽和脂肪酸, | zh_TW |
dc.subject.keyword | Copepoda,Tigriopus japonicus,DHA,EPA, | en |
dc.relation.page | 60 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2009-08-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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