請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22853
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蘇南維(Nan-Wei Su) | |
dc.contributor.author | Wei-Chia Chang | en |
dc.contributor.author | 張維家 | zh_TW |
dc.date.accessioned | 2021-06-08T04:30:46Z | - |
dc.date.copyright | 2009-12-29 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-11-30 | |
dc.identifier.citation | 方俊仁 (2008) 由heloferax spp.生產C50類葫蘿蔔素之研究. 國立台灣大學生物資源暨農學院農業化學系碩士論文.
Arakane K., Mizutani Y., and Sakata O. (2005) Preventive effects of carotenoids on photoaging and its application for cosmetics. J. Jpn. Cosmetic Sci. Soc. 29: 9-19. Armenta R. E., and Guerrero-Legarreta I. (2009) Stability studies on astaxanthin extracted from fermented shrimp byproducts. J Agric Food Chem. 57: 6095-6100. Bialonska D., Kasimsetty S. G., Schrader K. K., and Ferreira D. (2009) The effect of pomegranate (Punica granatum L.) byproducts and ellagitannins on the growth of human gut bacteria. J Agric Food Chem. 57: 8344-8349. Cerda B., Ceron J. J., Tomas-Barberan F. A., and Espin J. C. (2003) Repeated oral administration of high doses of the pomegranate ellagitannin punicalagin to rats for 37 days is not toxic. J Agric Food Chem. 51: 3493-3501. Cerda B., Espin J. C., Parra S., Martinez P., and Tomas-Barberan F. A. (2004) The potent in vitro antioxidant ellagitannins from pomegranate juice are metabolised into bioavailable but poor antioxidant hydroxy-6H-dibenzopyran-6-one derivatives by the colonic microflora of healthy humans. Eur J Nutr. 43: 205-220. Cerda B., Espin J. C., Parra S., Martinez P., and Tomas-Barberan F. A. (2005) Identification of urolithin A as a metabolite produced by human colon microflora from ellagic acid and related compounds. J Agric Food Chem. 53: 5571-5576. Cerda B., Llorach R., Ceron J. J., Espin J. C., and Tomas-Barberan F. A. (2003) Evaluation of the bioavailability and metabolism in the rat of punicalagin, an antioxidant polyphenol from pomegranate juice. Eur J Nutr. 42: 18-28. Cerda B., Tomas-Barberan F. A., and Espin J. C. (2005) Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans: identification of biomarkers and individual variability. J Agric Food Chem. 53: 227-235. Chung S. W., Ha Y. M., Kim Y. J., Song S., Lee H., Suh H., and Chung H. Y. (2009) Inhibitory effects of 6-(3-hydroxyphenyl)-2-naphthol on tyrosinase activity and melanin synthesis. Arch Pharm Res. 32: 289-294. Doyle B., and Griffiths L. A. (1980) The metabolism of ellagic acid in the rat. Xenobiotica. 10: 247-256. El-Aroud K. A., Abushoffa A. M., and Abdellatef H. E. (2007) Spectrophotometric and spectrofluorimetric methods for the determination of tranexamic acid in pharmaceutical formulation. Chem Pharm Bull (Tokyo). 55: 364-367. Fong N. J. C., Burgess M. L., Barrow K. D., and Glenn D. R. (2001) Carotenoid accumulation in the psychrotrophic bacterium Arthrobacter agilis in response to thermal and salt stress. Appl Microbiol Biot. 56: 750-756. Fujii T., and Saito M. (2009) Inhibitory effect of quercetin isolated from rose hip (Rosa canina L.) against melanogenesis by mouse melanoma cells. Biosci Biotechnol Biochem. 73: 1989-1993. Grant W. D., Kamekura M., Mcgenity T. J., and Ventosa A. (2001) Order Ⅰ. Halobacteriales. In D. R. Boone, and R. W. Castenholz (eds.). Order Ⅰ. Halobacteriales Hakkinen S. H., Karenlampi S. O., Heinonen I. M., Mykkanen H. M., and Torronen A. R. (1999) Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. J Agric Food Chem. 47: 2274-2279. Hori I., Nihei K., and Kubo I. (2004) Structural criteria for depigmenting mechanism of arbutin. Phytother Res. 18: 475-479. Hunt G., Todd C., Cresswell J. E., and Thody A. J. (1994) Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes. J Cell Sci. 107 ( Pt 1): 205-211. Hyun S. K., Lee W. H., Jeong da M., Kim Y., and Choi J. S. (2008) Inhibitory effects of kurarinol, kuraridinol, and trifolirhizin from Sophora flavescens on tyrosinase and melanin synthesis. Biol Pharm Bull. 31: 154-158. Inoue T., Shiota Y., and Yoshizawa K. (2008) Quantum chemical approach to the mechanism for the biological conversion of tyrosine to dopaquinone. J Am Chem Soc. 130: 16890-16897. Itoh K., Hirata N., Masuda M., Naruto S., Murata K., Wakabayashi K., and Matsuda H. (2009) Inhibitory effects of Citrus hassaku extract and its flavanone glycosides on melanogenesis. Biol Pharm Bull. 32: 410-415. Jackman M. P., Huber M., Hajnal A., and Lerch K. (1992) Stabilization of the oxy form of tyrosinase by a single conservative amino acid substitution. Biochem J. 282 ( Pt 3): 915-918. Jimenez-Cervantes C., Solano F., Kobayashi T., Urabe K., Hearing V. J., Lozano J. A., and Garcia-Borron J. C. (1994) A new enzymatic function in the melanogenic pathway. The 5,6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J Biol Chem. 269: 17993-18000. Johnston D., Orlow S. J., Levy E., and Bystryn J. C. (1992) Induction of B16 melanoma melanogenesis by a serum-free synthetic medium. Exp Cell Res. 201: 91-98. Kim Y. J., Kim Y. A., and Yokozawa T. (2009) Protection against oxidative stress, inflammation, and apoptosis of high-glucose-exposed proximal tubular epithelial cells by astaxanthin. J Agric Food Chem. 57: 8793-8797. Kornhauser A., Wei R. R., Yamaguchi Y., Coelho S. G., Kaidbey K., Barton C., Takahashi K., Beer J. Z., Miller S. A., and Hearing V. J. (2009) The effects of topically applied glycolic acid and salicylic acid on ultraviolet radiation-induced erythema, DNA damage and sunburn cell formation in human skin. J Dermatol Sci. 55: 10-17. Kushner D.J. (1978) Life in high salt and solute concentrations: halophilic bacteria. In D. J. Kushner (ed.). Life in high salt and solute concentrations: halophilic bacteria. Academic Press London. Larrosa M., Gonzalez-Sarrias A., Garcia-Conesa M. T., Tomas-Barberan F. A., and Espin J. C. (2006) Urolithins, ellagic acid-derived metabolites produced by human colonic microflora, exhibit estrogenic and antiestrogenic activities. J Agric Food Chem. 54: 1611-1620. Larrosa M., Gonzalez-Sarrias A., Yanez-Gascon M. J., Selma M. V., Azorin-Ortuno M., Toti S., Tomas-Barberan F., Dolara P., and Espin J. C. (2009) Anti-inflammatory properties of a pomegranate extract and its metabolite urolithin-A in a colitis rat model and the effect of colon inflammation on phenolic metabolism. J Nutr Biochem. Lazrak T., Wolff G., Albrecht A. M., Nakatani Y., Ourisson G., and Kates M. (1988) Bacterioruberins reinforce reconstituted halobacterium lipid-membranes. Biochim Biophys Acta 939: 160-162. Lerner A. B., and Fitzpatrick T. B. (1950) Biochemistry of melanin formation. Physiol Rev. 30: 91-126. Liu S. H., Pan I. H., and Chu I. M. (2007) Inhibitory effect of p-hydroxybenzyl alcohol on tyrosinase activity and melanogenesis. Biol Pharm Bull. 30: 1135-1139. Losso J. N., Bansode R. R., Trappey A., 2nd, Bawadi H. A., and Truax R. (2004) In vitro anti-proliferative activities of ellagic acid. J Nutr Biochem. 15: 672-678. Lu Z. R., Shi L., Wang J., Park D., Bhak J., Yang J. M., Park Y. D., Zhou H. W., and Zou F. (2009) The effect of trifluoroethanol on tyrosinase activity and conformation: inhibition kinetics and computational simulations. Appl Biochem Biotechnol. In press. Maddaluno J. F., and Faull K. F. (1988) Inhibition of mushroom tyrosinase by 3-amino-L-tyrosine: molecular probing of the active site of the enzyme. Experientia. 44: 885-887. Maeda K., and Fukuda M. (1996) Arbutin: mechanism of its depigmenting action in human melanocyte culture. J Pharmacol Exp Ther. 276: 765-769. Masatoshi, A. N. Hiroaki, T. Keniji D. Hiroshi, and Keiichiro I. (1991) Agent containing an ellagic acid series compound for external application and use thereof. Patent No.:US05073545. Mukhtar H., Das M., and Bickers D. R. (1986) Inhibition of 3-methylcholanthrene-induced skin tumorigenicity in BALB/c mice by chronic oral feeding of trace amounts of ellagic acid in drinking water. Cancer Res. 46: 2262-2265. Nakayasu M., Saeki H., Tohda H., and Oikawa A. (1977) Effects of sugars on melanogenesis in cultured melanoma cells. J Cell Physiol. 92: 49-55. Nishioka K. (1978) Particulate tyrosinase of human malignant melanoma. Solubilization, purification following trypsin treatment, and characterization. Eur J Biochem. 85: 137-146. Ohguchi K., Ito M., Yokoyama K., Iinuma M., Itoh T., Nozawa Y., and Akao Y. (2009) Effects of sesquiterpene lactones on melanogenesis in mouse B16 melanoma cells. Biol Pharm Bull. 32: 308-310. Oka M., Nagai H., Ando H., Fukunaga M., Matsumura M., Araki K., Ogawa W., Miki T., Sakaue M., Tsukamoto K., Konishi H., Kikkawa U., and Ichihashi M. (2000) Regulation of melanogenesis through phosphatidylinositol 3-kinase-Akt pathway in human G361 melanoma cells. J Invest Dermatol. 115: 699-703. Park K. H., Park Y. D., Lee J. R., Hahn H. S., Lee S. J., Bae C. D., Yang J. M., Kim D. E., and Hahn M. J. (2005) Inhibition kinetics of mushroom tyrosinase by copper-chelating ammonium tetrathiomolybdate. Biochim Biophys Acta. 1726: 115-120. Parvez S., Kang M., Chung H. S., Cho C., Hong M. C., Shin M. K., and Bae H. (2006) Survey and mechanism of skin depigmenting and lightening agents. Phytother Res. 20: 921-934. Prabhu P. N., Ashokkumar P., and Sudhandiran G. (2009) Antioxidative and antiproliferative effects of astaxanthin during the initiation stages of 1,2-dimethyl hydrazine-induced experimental colon carcinogenesis. Fundam Clin Pharmacol. 23: 225-234. Pretsch W., and Favor J. (2007) Genetic, biochemical, and molecular characterization of nine glyceraldehyde-3-phosphate dehydrogenase mutants with reduced enzyme activity in Mus musculus. Mamm Genome. 18: 686-692. Priyadarsini K. I., Khopde S. M., Kumar S. S., and Mohan H. (2002) Free radical studies of ellagic acid, a natural phenolic antioxidant. J Agric Food Chem. 50: 2200-2206. Saito T., Miyabe Y., Ide H., and Yamamoto O. (1997) Hydroxyl radical scavenging ability of bacterioruberin. Radiat Phys Chem. 50: 267-269. Saito T., Terato H., and Yamamoto O. (1994) Pigments of Rubrobacter-Radiotolerans. Arch Microbiol. 162: 414-421. Schmaus G., Vielhaber G., Jacobs K., and Franke H. (2006) 4-(1-Phenylethyl) 1,3-benzenediol: a new highly potent lightening agent. J Cosmet Sci. 57: 197-198. Schroterova L., Kralova V., Voracova A., Haskova P., Rudolf E., and Cervinka M. (2009) Antiproliferative effects of selenium compounds in colon cancer cells: comparison of different cytotoxicity assays. Toxicol In Vitro. 23: 1406-1411. Sealy R. C., Hyde J. S., Felix C. C., Menon I. A., and Prota G. (1982) Eumelanins and pheomelanins: characterization by electron spin resonance spectroscopy. science. 217: 545-547. Shahmohammadi H. R., Asgarani E., Terato H., Saito T., Ohyama Y., Gekko K., Yamamoto O., and Ide H. (1998) Protective roles of bacterioruberin and intracellular KCl in the resistance of Halobacterium salinarium against DNA-damaging agents. Journal of Radiation Research (Tokyo). 39: 251-262. Shimogaki H., Tanaka Y., Tamai H., and Masuda M. (2000) In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition. Int J Cosmet Sci. 22: 291-303. Singh B. N., Singh B. R., Singh R. L., Prakash D., Dhakarey R., Upadhyay G., and Singh H. B. (2009) Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem Toxicol. 47: 1109-1116. Song H. S., and Sim S. S. (2009) Acetoside inhibits alpha-MSH-induced melanin production in B16 melanoma cells by inactivation of adenyl cyclase. J Pharm Pharmacol. 61: 1347-1351. Thresiamma K. C., and Kuttan R. (1996) Inhibition of liver fibrosis by ellagic acid. Indian J Physiol Pharmacol. 40: 363-366. Tsuji-Naito K., Hatani T., Okada T., and Tehara T. (2007) Modulating effects of a novel skin-lightening agent, alpha-lipoic acid derivative, on melanin production by the formation of DOPA conjugate products. Bioorg Med Chem. 15: 1967-1975. Vielhaber G., Schmaus G., Jacobs K., Franke H., Lange S., Herrmann M., Joppe H., and Koch O. (2007) 4-(1-phenylethyl)1,3-benzenediol: a new, highly efficient lightening agent. Int J Cosmet Sci. 29: 65-66. Yoshimura M., Watanabe Y., Kasai K., Yamakoshi J., and Koga T. (2005) Inhibitory effect of an ellagic acid-rich pomegranate extract on tyrosinase activity and ultraviolet-induced pigmentation. Biosci Biotechnol Biochem. 69: 2368-2373. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22853 | - |
dc.description.abstract | 本研究係探討bacterioruberin以及鞣花酸代謝物urolithin A、urolithin B作為美白化妝品應用新素材之可行性。以小鼠黑色素瘤細胞株B16F0做為試驗目標物,將上述材料分別與其共培養後,得到細胞存活率達80%以上之最高濃度,並定此濃度為最高測試濃度,再以此濃度處理B16F0,探討其對黑色素生成量、對B16F0酪胺酸酶的活性之抑制、以及對B16F0酪胺酸酶mRNA之表現等的影響情形,作為判斷urolithin A和urolithin B以及bacterioruberin是否具有作為美白素材的潛力。試驗結果顯示,最高測試濃度分別為urolithin A 10 μM、urolithin B 10 μM與bacterioruberin 3.75 nM。以上述之濃度分別處理B16F0(培養基為含測試樣品之5 %胎牛血清的DMEM,持續培養72小時),可減少黑色素生成達23%以上。Urolithin A和urolithin B以及bacterioruberin可有效抑制B16F0酪胺酸酶與蕈酪胺酸酶活性。B16F0分別與urolithin A和urolithin B以及bacterioruberin共培養72小時後,不會影響B16F0之酪胺酸酶mRNA的表現量,顯示這些測試樣品對B16F0黑色素生成能力的抑制係直接來自於測試樣品對酪胺酸酶活性之抑制作用。進一步探討上述測試樣品與酪胺酸酶之作用機制,結果顯示urolithin A和urolithin B以及bacterioruberin抑制B16F0酪胺酸酶與基質L-DOPA之模式為競爭型之抑制反應。 | zh_TW |
dc.description.abstract | This study was to find out the potential of urolithin A, urolithin B and bacterioruberin in whitening application of cosmetics. B16F0 cells were employed as the tested cells for evaluating the potent use of above three samples. B16F0 cells were treated by various concentrations of urolithin A, urolithin B and bacterioruberin. The concentrations of three samples, made the viability of B16F0 cells higher than 80% , were set as the highest concentration for further tests. The experimental works including the inhibition of melanin formation and tyrosinase activity of B16F0 cells as well as the corresponding mRNA expression of B16F0 tyrosinase were conducted in this study. The results revealed that the highest concentrations of three samples, without significant cytotoxic effect, were respective 10 μM for urolithin A, 10 μM for urolithin B and 3.75 nM for bacterioruberin, and the above concentrations of three samples incubated with B16F0 for 72 h could effectively reduce the melanin formation more than 23% in B16F0 cells. Urolithin A, urolithin B and bacterioruberin could not only inhibit the activity of B16F0 tyrosinase, but also inhibit the activity of mushroom tyrosinase. In addition, the results of RT-PCR revealed that there were no significant influences on the mRNA expression of B16F0 tyrosinase. Therefore, we suggested that the melanin formation of B16F0 cells reduced by urolithin A, urolithin B and bacterioruberin could be attributed their capabillity to act directly with the tyrosinase of cells. Furthermore, the results from the enzyme kinetics studies of tyrosinase inhibited by urolithin A, urolithin B and bacterioruberin revealed that all tested samples were the same mode of competitive inhibition toward the L-DOPA substrate of B16F0 tyrosinase. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:30:46Z (GMT). No. of bitstreams: 1 ntu-98-R96623026-1.pdf: 901074 bytes, checksum: 2142f15b65043054f3f3fb8f90afa9e6 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 口試委員會審定書 i
致謝 ii 中文摘要 iii Abstract iv 縮寫表 vi 第二章、文獻回顧 2 壹、鞣花酸之基本性質 2 貳、自然界之鞣花酸 2 参、鞣花酸的生理活性 2 一、抗癌力 2 二、抗氧化力 3 三、抑菌力 3 四、美白功效 3 第二節、Urolithin A與urolithin B 6 壹、Urolithin A與urolithin B之基本性質 6 貳、Urolithin A與urolithin B代謝的研究 6 参、Urolithin A與urolithin B生理活性 7 第三節、菌紅素(Bacterioruberin) 11 壹、菌紅素之基本性質 11 貳、菌紅素的生理活性 11 参、菌紅素的還原力及自由基清除能力 12 第四節、蝦紅素(Astaxanthin) 14 壹、蝦紅素之基本性質 14 貳、蝦紅素的生理活性 14 一、抗氧化與抗癌 14 二、美白功效 15 第五節、黑色素 17 壹、黑色素之生成 17 貳、美白成分之功效 18 一、抑制酪胺酸酶活性 18 二、還原與淡化黑色素 18 第三章、材料與方法 21 第一節、實驗架構 21 第二節、實驗材料 22 壹、生物性材料 22 貳、試藥 22 第三節、儀器設備 24 第四節、實驗方法 25 壹、B16F0之培養與細胞毒性測試 25 一、B16F0之型態與培養條件 25 二、細胞毒性測試 25 貳、B16F0黑色素生成及生成量指標 26 一、B16F0黑色素穩定生成條件之探討 26 二、B16F0黑色素生成之測定與黑色素生成抑制性試驗 26 参、Bacterioruberin、urolithin A及B對B16F0與蕈酪胺酸酶活性之抑制試驗 27 一、B16F0酪胺酸酶活性測試 27 1. B16F0細胞酪胺酸酶的製備 27 2. Bacterioruberin、urolithin A及B對B16F0細胞酪胺酸酶的活性之抑制試驗 27 二、Bacterioruberin、urolithin A及B對蕈酪胺酸酶的活性之抑制試驗 29 肆、Bacterioruberin、urolithin A及B對B16F0酪胺酸酶mRNA表現量之影響 30 一、B16F0 total RNA之製備: 30 二、DNase處理: 30 三、MMLV High Performance Reverse Transcriptase: 31 四、聚合酶連鎖反應: 31 五、Bacterioruberin、urolithin A及B對於B16F0酪胺酸酶之作用機制 32 六、數據之統計分析 32 第四章、結果與討論 33 第一節、B16F0黑色素穩定生成之程序建立 33 第二節、細胞毒性測試 35 第三節、B16F0黑色素生成之測定 39 第四節、Bacterioruberin、urolithin A及B對蕈酪胺酸酶活性抑制之影響 42 第五節、Bacterioruberin、urolithin A及B對B16F0酪胺酸酶活性抑制之影響 44 第六節、Bacterioruberin、urolithin A及B對B16F0酪胺酸酶mRNA表現量之影響 46 第七節、Bacterioruberin、urolithin A及B對於酪胺酸酶作用之動力學機制 50 第五章、結論 55 第六章、參考文獻 56 附錄一、美白成分之結構 64 附錄二、DMEM之成分 66 附錄三、菌紅素的分離與純化流程圖 67 附錄四、Urolithin A與urolithin B之HPLC分析與吸收光譜 68 附錄五、GAPDH基因之不同PCR循環數電泳圖比較 70 | |
dc.language.iso | zh-TW | |
dc.title | Bacterioruberin和鞣花酸代謝物urolithin A、urolithin B抑制B16F0黑色素形成之研究 | zh_TW |
dc.title | Effects of bacterioruberin and ellagic acid metabolites,urolithin A and urolithin B on the melanin formation in B16F0 cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李敏雄(Min-Hsiung Lee),洪傳揚(Chwan-Yang Hong),鍾玉明(Yu-Ming Chung),林銘澤(Ming-Tse Lin) | |
dc.subject.keyword | urolithin A,urolithin B,bacterioruberin, | zh_TW |
dc.relation.page | 70 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2009-12-01 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 879.96 kB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。