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Title: | 薏仁麩皮乙醇萃取物之活性篩選及其乙酸乙酯區分層之化學組成研究 Screening of Physiological Activities on Adlay Bran Ethanolic Extract and Investigation on Chemical Composition of Its Ethyl Acetate Fraction |
Authors: | Cheng-Pei Chung 鍾成沛 |
Advisor: | 江文章 |
Keyword: | 薏仁麩皮,乙酸乙酯區分層,螺旋內醯胺,螺旋烯酮,高效液相層析串聯質譜,部分純化物, adlay bran,ethyl acetate fraction,spirolactam,spiroenone,high-performance liquid chromatography-mass spectrum,partially purified fraction, |
Publication Year : | 2011 |
Degree: | 博士 |
Abstract: | 薏苡(Coix lachryma-jobi L. var. ma-yuen Stapf)在藥典記載具有利尿、抗腫瘤、舒緩糖尿病、健胃等功效,其可食部位糙薏仁(dehulled adlay, DA)由麩皮(adlay bran, AB)與胚乳組成,是東方各國普遍使用之藥食兼備的食材。近年研究指出其具有抑制癌細胞增生(anti-proliferation)、抗過敏(anti-allergy)與抗發炎(anti-inflammation)之活性,但其化學組成資料目前仍然不足。本研究使用DA乙醇萃取物(DA ethanolic extract, DAE)、AB乙醇萃取物(AB ethanolic extract, ABE)與精白薏仁乙醇萃取物(polished adlay etanolic extract, PAE)進行抑制各種癌細胞增生之活性篩選,其中以ABE最具活性且在其區分層中以乙酸乙酯區分層(ethyl acetate fraction of ABE, ABE-EA)效果最顯著,進而自其中分離到33個化合物,其中2個螺旋內醯胺(spirolactam)與1個螺旋烯酮(spiroenone)為新化合物。接續使用這些物質與以往曾經在AB中被發現的成分進行抑制癌細胞生長、降低去顆粒化效應與抗發炎之活性篩選,結果顯示最具生理活性者為類黃酮,其中以柚皮素(naringenin)、金聖草素(chrysoeriol)與多甲氧基化黃酮(polymethoxyflavones, PMFs)活性最佳;此外螺旋內醯胺對諸多癌細胞亦均具抑制效用,對於肝癌尤其顯著,而ABE中的酯化態植物固醇則具有優良的抑制去顆粒化活性。為了比較薏苡籽實各部位的化學組成,利用高效液相層析(high-performance liquid chromatography, HPLC)-串聯質譜(mass spectrum, MS)建立分析平台以供定性,結果顯示薏苡殼(adlay hull, AH)中也具有豐富的PMFs。本研究的活性篩選結果、AB之分離純化資料與HPLC-MS方法將可應用在分析AB相關之機能性部分純化物,並作為其保健素材之品管基礎。 Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is recorded as diuric, antitumor, anti-diabetes and stomachic agent. Edible parts of adlay seeds, dehulled adlay (DA), are composed of adlay bran (AB) and endosperm, and are regarded as food and medicine in Eastern world. DA was reported to possess anti-proliferative, anti-allergic, and anti-inflammatory activities, but it is still insufficient of its chemical composition. In the present study, DA ethanolic extract (DAE), AB ethanolic extract (ABE), and polished adlay ethanolic extract (PAE) were prepared, and screened for anti-proliferative capacities on cancer cell lines. ABE was active comprehensively, and 33 compounds were purified from most effective ethyl acetate fraction of ABE (ABE-EA), including 2 new spirolactams and 1 new spiroenone. The components that have been reported in AB and that isolated in this study were examined for anti-proliferative, anti-degranulation, and anti-inflammatory activities. Among them, flavonoids in ABE-EA, such as naringenin, chrysoeriol, and polymethoxyflavones (PMFs) were superior. Besides, cancer cells were inhibited by spirolactams especially hepatomas; while esterified phytosterols reduced degranulation significantly. A high-performance liquid chromatography (HPLC)-mass spectrum (MS) analytical platform was established, and PMFs were also corresponded in adlay hull (AH). The results of biofunctional screening, chemical composition in AB and HPLC-MS method may be utilized in developing AB-related partially purified fraction. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22698 |
Fulltext Rights: | 未授權 |
Appears in Collections: | 食品科技研究所 |
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