請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22463
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 齊肖琪 | |
dc.contributor.author | Chen-Fu Xing | en |
dc.contributor.author | 刑辰馥 | zh_TW |
dc.date.accessioned | 2021-06-08T04:18:22Z | - |
dc.date.copyright | 2010-07-28 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-26 | |
dc.identifier.citation | Acosta, F., Vivas, J., Padilla, D., Vega, J., Bravo, J., Grasso, V., and Real, F. (2009). Invasion and survival of Photobacterium damselae subsp.
piscicida in non-phagocytic cells of gilthead sea bream, Sparus aurata L. J Fish Dis 32, 535-541. Alakomi, H., Skytta, E., Saarela, M., Mattila-Sandholm, T., Latva-Kala, K., and Helander, I. (2000). Lactic acid permeabilizes gram-negative bacteria by disrupting the outer membrane. Appl Environ Microbiol 66, 2001-2005. Aly, S.M., Abdel-Galil Ahmed, Y., Abdel-Aziz Ghareeb, A., and Mohamed, M.F. (2008). Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish Shellfish Immunol 25, 128-136. Arijo, S., Brunt, J., Chabrillon, M., Diaz-Rosales, P., and Austin, B. (2008). Subcellular components of Vibrio harveyi and probiotics induce immune responses in rainbow trout, Oncorhynchus mykiss (Walbaum), against V. harveyi. J Fish Dis 31, 579-590. Balcazar, J.L., de Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vendrell, D., and Muzquiz, J.L. (2006). The role of probiotics in aquaculture. Vet Microbiol 114, 173-186. Balcazar, J.L., de Blas, I., Ruiz-Zarzuela, I., Vendrell, D., Calvo, A.C., Marquez, I., Girones, O., and Muzquiz, J.L. (2007a). Changes in intestinal microbiota and humoral immune response following probiotic administration in brown trout (Salmo trutta). Br J Nutr 97, 522-527. Balcazar, J.L., de Blas, I., Ruiz-Zarzuela, I., Vendrell, D., Girones, O., and Muzquiz, J.L. (2007b). Enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (Oncorhynchus mykiss). FEMS Immunol Med Microbiol 51, 185-193. Balcazar, J.L., Rojas-Luna, T., and Cunningham, D.P. (2007c). Effect of the addition of four potential probiotic strains onthe survival of pacific white shrimp (Litopenaeus vannamei) following immersion challenge with Vibrio parahaemolyticus. J Invert Pathol 96, 147-150. Balcazar, J.L., Vendrell, D., de Blas, I., Ruiz-Zarzuela, I., Girones, O., and Muzquiz, J.L. (2007d). In vitro competitive adhesion and production of antagonistic compounds by lactic acid bacteria against fish pathogens. Vet Microbiol 122, 373-380. Barnes, A.C., Balebona, M.C., Horne, M.T., and Ellis, A.E. (1999). Superoxide dismutase and catalase in Photobacterium damselae subsp. piscicida and their roles in resistance to reactive oxygen species. Microbiology 145, 483-494. Cammarota, M., De Rosa, M., Stellavato, A., Lamberti, M., Marzaioli, I., and Giuliano, M. (2009). In vitro evaluation of Lactobacillus plantarum DSMZ 12028 as a probiotic: emphasis on innate immunity. Int J Food Microbiol 135, 90-98. Collado, M.C., Meriluoto, J., and Salminen, S. (2007). Development of new probiotics by strain combinations: Is it possible to improve the adhesion to intestinal mucus? J Dairy Sci 90, 2710-2716. Choudhury, D., Pal, A.K., Sahu, N.P., Kumar, S., Das, S.S., and Mukherjee, S.C. (2005). Dietary yeast RNA supplementation reduces mortality by Aeromonas hydrophila in rohu (Labeo rohita L.) juveniles. Fish Shellfish Immunol 19, 281-291. Cotter, P.D., Hill, C., and Ross, R.P. (2005). Bacteriocins: developing innate immunity for food. Nat Rev Microbiol 3, 777-788. Dalmin, G., Kathiresan, K., and Purushothaman, A. (2001). Effect of probiotics on bacterial population and health status of shrimp in culture pond ecosystem. Indian J Exp Biol 39, 939-942. D'Arienzo, R., Maurano, F., Lavermicocca, P., Ricca, E., and Rossi, M. (2009). Modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity. Cytokine 48, 254-259. Delcenserie, V., Martel, D., Lamoureux, M., Amiot, J., Boutin, Y., and Roy, D. (2008). Immunomodulatory effects of probiotics in the intestinal tract. Curr Issues Mol Biol 10, 37-54. De Man, C,, Rogosa, M., and Sharpe, E. (1960). A medium for the cultivation of lactobacilli. J Appl Bacteriol 23, 130-135. Dos Santos, N.M.S., Taverne-Thiele, J.J., Barnes, A.C., Van Muiswinkel, W.B., Ellis, A.E., and Rombout, J.H.W.M. (2001). The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp. piscicida bacterin: An ontogenetic study. Fish Shellfish Immunol 11, 65-74. Egusa, S. (1983). Disease problems in cultured yellowtail, Seriola quinqueradiata, culture: a review. Rapports et proces-verbaux des reunions Conseil International pour l'exploration de la mer 182, 10-18. Elkamel, A.A., and Thune, R.L. (2003). Invasion and replication of Photobacterium damselae subsp. piscicida in fish cell lines. J Aquat Anim Health 15, 167-174. Fang, Y.C. (2009). The impacts of different immunization strategies of inactivated Photobacterium damselae subsp. piscicida on cobia specific immune responses. Master thesis, Institute of Zoology, National Taiwan University. Fukuda, Y. and Kusuda, R. (1981). Efficacy of vaccination for pseudotuberculosis in cultured yellowtail by various routes of administration. Bull Japan Soc Sci Fish 47, 147-150. Fuller R. (1987). A review, probiotics in man and animals. J Appl Bacteriol 66, 365-378. Gauthier, G., Lafay, B., Ruimy, R., Breittmayer, V., Nicolas, J.L., Gauthier, M., and Christen, R. (1995). Small-subunit rRNA sequences and whole DNA relatedness concur for the reassignment of Pasteurella piscicida (Snieszko et al.) Janssen and Surgalla to the genus Photobacterium as Photobacterium damsela subsp. piscicida comb. nov. Int J Syst Bacteriol 45, 139-144. Hawke, J.P., Plakas, S.M., Minton, R.V., McPhearson, R.M., Snider, T.G., and Guarino, A.M. (1987). Fish pasteurellosis of cultured striped bass (Morone saxatilis) in coastal Alabama. . Aquaculture 65, 193-204. Holzapfel, W.H., and Wood, B.J.B. (1998). The genera of lactic acid bacteria (1st ed.). London Blackie Academic & Professional. Irianto, A., and Austin, B. (2002). Probiotics in aquaculture. J Fish Dis 25, 633-642. Iwana, G., and Nakanishi, T. (1996). The fish immune system, organism, pathogeand enviroment. Fish physiol 15. Kalchayanand, N., Hanlin M.B., Ray, B. (1992) Sublethal injury makes Gram-negative and resistant Gram-positive bacteria sensitive to the bacteriocins, pediocin AcH and nisin. Lett Appl Microbiol 15, 239-243. Kawakami, H., Shinohara, N., Fukuda, Y., Yamashita, H., Kihara, H., and Sakai, M. (1997). The efficacy of lipopolysaccharide mixed chloroform-killed cell (LPS-CKC) bacterin of Pasteurella piscicida on yellowtail, Seriola quinqueradiata. Aquaculture 154, 95-105. Kim, D.H., and Austin, B. (2006). Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics. Vet Immunol Immunopathol 114, 297-304. Ku, C.C. (2007). Handbook of cobia. Taiwan Fisheries Economic Development Association. 8-11. Ku, C.C., Wang, C.S., and Lu, C.H. (2008). A vaccination trial using attenuated Photobacterium damselae ssp. piscicida in cobia. J Fish Soc Taiwan 35, 127-131. Ku, C.C. (2009). In vitro antimicrobial susceptibility of Photobacterium damselae ssp. piscicida from cobia (Rachycentron cannadum) at Penghu (Pescodores) Island, Taiwan during 1999-2008. J Fish Soc Taiwan 36, 151-160. Kusuda, R., and Hamaguchi, M. (1987). A comparative study on efficacy of immersion and combination of immersion and oral vaccination methods against pseudotuberculosis in yellowtail. Bull JPN Soc Sci Fish 53, 1005-1008. Kusuda, R., and Hamaguchi, M. (1988). The efficacy of attenuated live bacterin of Pasteurella piscicida against pseudotuberculosis in yellowtail. Bull Eur Ass Fish Pathol 8, 51-53. Lee, K.K., and Ellis, A.E. (1990). Glycerophospholipid: cholesterol acyltransferase complexed with lipopolysaccharide (LPS) is a major lethal exotoxin and cytolysin of Aeromonas salmonicida: LPS stabilizes and enhances toxicity of the enzyme. J Bacteriol 172, 5385-5393. Lee, K.K., and Ellis, A.E. (1991). The role of the lethal extracellular cytolysin of Aeromonas salmonicida in the pathology of furunculosis. J Fish Dis 14, 453-460. Liao, I.C., Huang, T.S., Tsai, W.S., Hsueh, C.M., Chang, S.L., and Leano, E.M. (2004). Cobia culture in Taiwan: current status and problems. Aquaculture 237, 155-165. Lin, J.H.Y., Chen, T.Y., Chen, M.S., Chen, H.E., Chou, R.L., Chen, T.I., Su, M.S., and Yang, H.L. (2006). Vaccination with three inactivated pathogens of cobia (Rachycentron canadum) stimulates protective immunity. Aquaculture 255, 125-132. Liu, P.C., Lin, J.I.Y., and Lee, K.K. (2003). Virulence of Photobacterium damselae subsp. piscicida in cultured cobia Rachycentron canadum. J Basic Microbiol 43, 499-507. Lopez-Doriga, M.V., Barnes, A.C., Dos Santos, N.M.S., and Ellis, A.E. (2000). Invasion of fish epithelial cells by Photobacterium damselae subsp. piscicida : Evidence for receptor specificity, and effect of capsule and serum. Microbiology 146, 21-30. Magarinos, B., Romalde, J.L., Bandin, I., Fouz, B., and Toranzo, A.E. (1992). Phenotypic, Antigenic, and Molecular Characterization of Pasteurella piscicida Strains Isolated from Fish. App Environ Microbiol 58, 3316-3322. Magarinos, B., Toranzo, A.E., and Romalde, J.L. (1996). Phenotypic and pathobiological characteristics of Pasteurella piscicida. Annu Rev Fish Dis 6, 41-64. Mathur, S., and Singh, R. (2005). Antibiotic resistance in food lactic acid bacteria - a review. Int J Food Microbiol 105, 281-295. Montville, T.J., and Bruno, M.E. (1994). Evidence that dissipation of proton motive force is a common mechanism of action for bacteriocins and other antimicrobial proteins. Int J Food Microbiol 24, 53-74. Munro, A.L.S., Hasting, T.S., Ellis. A.E. and Liversidge, J. (1980) Studies on an ichthyotoxic material produced extracellularly by the furnculosis bacterium, Aeromonas salmonicida. In: Proceedings of the third session of the EIPAC (FAO/OIE) CPRA: Fish Disease, 98-106. Nelson, J.S., Kawahala, E., Kawai, K., and Kusuda, R. (1989). Macrophage infiltration in pseudotuberculosis of yellowtail, Seriola quinqueradiata. Bull Mar sci Fish kochi Univ 11, 17-22. Nikoskelainen, S., Ouwehand, A., Salminen, S., and Bylund, G. (2001). Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus. Aquaculture 198, 229-236. Nikoskelainen, S., Ouwehand, A.C., Bylund, G., Salminen, S., and Lilius, E. (2003). Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish Shellfish Immunol 15, 443-452. Osorio, C.R., Collins, M.D., Toranzo, A.E., Barja, J.L., and Romalde, J.L. (1999). 16S rRNA gene sequence analysis of Photobacterium damselae and Nested PCR method for rapid detection of the causative agent of fish pasteurellosis. Appl Environ Microbiol 65, 2942-2946. Paineau, D., Carcano, D., Leyer, G., Darquy, S., Alyanakian, M.A., Simoneau, G., Bergmann, J.F., Brassart, D., Bornet, F., and Ouwehand, A.C. (2008). Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial. FEMS Immunol Med Microbiol 53, 107-113. Panigrahi, A., Kiron, V., Kobayashi, T., Puangkaew, J., Satoh, S., and Sugita, H. (2004). Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Vet Immunol Immunopathol 102, 379-388. Panigrahi, A., Kiron, V., Satoh, S., Hirono, I., Kobayashi, T., Sugita, H., Puangkaew, J., and Aoki, T. (2007). Immune modulation and expression of cytokine genes in rainbow trout Oncorhynchus mykiss upon probiotic feeding. Dev Comp Immunol 31, 372-382. Prieur, G., Nicolas, J.L., Plusquellec, A., and Vigneulle, M. (1990). Interactions between bivalves molluscs and bacteria in the marine environment. Oceanogr Mar Biol Annu Rev 28, 227-352. Puangkaew, J., Kiron, V., Somamoto, T., Okamoto, N., Satoh, S., Takeuchi, T., and Watanabe, T. (2004). Nonspecific immune response of rainbow trout (Oncorhynchus mykiss Walbaum) in relation to different status of vitamin E and highly unsaturated fatty acids. Fish Shellfish Immunol 16, 25-39. Rajan, P.R., Lin, J.H.Y., Ho, M.S., and Yang, H.L. (2003). Simple and rapid detection of Photobacterium damselae ssp. piscicida by a PCR technique and plating method. J App Microbiol 95, 1375-1380. Rescigno, M., Urbano, M., Valzasina, B., Francolini, M., Rotta, G., Bonasio, R., Granucci, F., Kraehenbuhl, J.P., and Ricciardi-Castagnoli, P. (2001). Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria. Nat Immunol 2, 361-367. Robertson, P.A.W., O'Dowd, C., Burrells, C., Williams, P., and Austin, B. (2000). Use of Carnobacterium sp. as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum). Aquaculture 185, 235-243. Romalde, J.L. (2002). Photobacterium damselae subsp. piscicida: An integrated view of a bacterial fish pathogen. Int Microbiol 5, 3-9. Salinas, I., Myklebust, R., Esteban, M.A., Olsen, R.E., Meseguer, J., and Ringo, E. (2008). In vitro studies of Lactobacillus delbrueckii subsp. lactis in Atlantic salmon (Salmo salar L.) foregut: tissue responses and evidence of protection against Aeromonas salmonicida subsp. salmonicida epithelial damage. Vet Microbiol 128, 167-177. Salminen, S., Ouwehand, A., Benno, Y. and Lee, Y.K. (1999). Probiotics: how should they be defined? Trends Food Sci Technol 10, 107-110. Santos, Y., Romalde, J.L., Bandin, I., Magarinos, B., Nunez, S., Barja, J.L., and Toranzo, A.E. (1993). Usefulness of the API-20E system for the identification of bacterial fish pathogens. Aquaculture 116, 111-120. Singh, V., Singh, K., Amdekar, S., Singh, D.D., Tripathi, P., Sharma, G.L., and Yadav, H. (2009). Innate and specific gut-associated immunity and microbial interference. FEMS Immunol Med Microbiol 55, 6-12. Sharifuzzaman, S.M., and Austin, B. (2009). Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol 108, 2162-2170. Sugita, H., Okano, R., Suzuki, Y., Iwai, S., Mizukami, M., Akiyama, N., and Matsuura, S. (2002). Antibacterial abilities of intestinal bacteria from larval and juvenile Japanese flounder against fish pathogens. Fisheries Sci 68, 1004-1011. Thune, R.L., Stanley, L.A. and Cooper, R.K. (1993). Pathogenesis of Gram-negative bacterial infections in warm-water fish. Annu Rev Fish Dis 3, 37-68. Toranzo, A.E., Barreiro, S., Casal, J.F., Figueras, A., Magariiios, B., and Barja, J.L. (1991). Pasteurellosis in cultured gilthead seabream (Sparus aurata): first report in Spain. Aquaculture 99, 1-15. Toranzo, A. E. and Barja, J.L. (1993). Virulence factors of bacteria pathogenic for coldwater fish. Ann Rev Fish Dis 3, 5-36. Tseng, D.Y., Ho, P.L., Huang, S.Y., Cheng, S.C., Shiu, Y.L., Chiu, C.S., and Liu, C.H. (2009). Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20. Fish Shellfish Immunol 26, 339-344. Tung, M.C., Tsai, S.S., Ho, L.F., Huang, S.T., and Chen, S.C. (1985). An acute septicemic infection of Pasteurella organism in pond-cultured Formosa snake-head fish (Channa maculata Lacepede) in Taiwan. Fish Pathol 20, 143-148. Vaseeharan, B., and Ramasamy, P. (2003). Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett Appl Microbiol 36, 83-87. Weisburg ,W., Barns, S., Pelletier, D., and Lane, D. (1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697-703. Zappulli, V., Patarnello, T., Patarnello, P., Frassineti, F., Franch, R., Manfrin, A., Castagnaro, M., and Bargelloni, L. (2005). Direct identification of Photobacterium damselae subspecies piscicida by PCR-RFLP analysis. Dis Aquat Org 65, 53-61. Zhang, W., Azevedo, M.S.P., Wen, K., Gonzalez, A., Saif, L.J., Li, G., Yousef, A.E., and Yuan, L. (2008). Probiotic Lactobacillus acidophilus enhances the immunogenicity of an oral rotavirus vaccine in gnotobiotic pigs. Vaccine 26, 3655-3661. Zhou, X., Tian, Z., Wang, Y., and Li, W. (2009). Effect of treatment with probiotics as water additives on tilapia (Oreochromis niloticus) growth performance and immune response. Fish Physiol Biochem, DOI10.1007/s10695-009-9320-z Zorrilla, I., Balebona, M.C., Morinigo, M.A., Sarasquete, C., and Borrego, J.J. (1999). Isolation and characterization of the causative agent of pasteurellosis, Photobacterium damsela ssp. piscicida, from sole, Solea senegalensis (Kaup). J Fish Dis 22, 167-172. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22463 | - |
dc.description.abstract | 海鱺(Rachycentron canadum)是台灣海水箱網水產養殖重要的魚種,然而 海鱺漁業受到發光桿菌症(photobacteriosis)嚴重危害,病原為美人魚發光桿菌 殺魚亞種(Photobacterium damselae subsp. piscicida, Pdp)。目前雖以抗生素及疫 苗來防範疫情,但效果仍有限。 本研究探討一株分離自海鱺腸道的乳酸菌 (Pediococcus pentosaceus strain 4012, LAB4012),於體外實驗中發現 LAB4012 的 胞外分泌物會抑制 Pdp 生長;而在 Pdp 浸泡攻毒實驗中,海鱺苗經餵食含 LAB4012 的飼料兩週後,其相對存活率(Relative percent survival, RPS)可達 80%,餵食四週後攻毒,相對存活率更高。而且餵食 LAB4012 兩週後,海鱺的成 長率比起沒有餵食的對照組高12%。雖然海鱺餵食 LAB4012 兩週後,無法增強周 邊血液白血球的吞噬活性(phagocytosis activity),但其呼吸爆活性(respiratory burst)卻顯著提高。以 Pdp 去活化疫苗免疫海鱺後,發現餵食 LAB4012 並不會 提高魚體內的專一性免疫反應,但在Pdp浸泡攻毒實驗中,卻可使免疫魚再提高 20%的存活率,造成加成保護效果的原因極可能與 LAB4012 所誘發之先天免疫反 應有關。綜合以上特性,證明 LAB4012 乳酸菌可作為海鱺抗 Pdp 感染之益生菌。 | zh_TW |
dc.description.abstract | Cobia (Rachycentron canadum) is an important fish species for marine-cage aquaculture in Taiwan. However, cobia industry has been severely damaged by photobacteriosis, which is caused by Photobacterium damselae subsp. piscicida (Pdp). Antibiotics and vaccines have been applied for the control of Pdp infection, but the efficacy is not consistent. In this study, one lactic acid bacteria, Pediococcus pentosaceus strain 4012 (LAB4012), was isolated from the intestine of cobia, was investigated, and it was found that the culture supernatant of LAB4012 was able to inhibit Pdp growth in vitro. In the Pdp immersion challenge test, the relative percentage of survival (RPS) in cobia fed with LAB4012 for 2 weeks was 80%, and it was even higher in cobia for 4-week LAB4012-feeding. Furthermore, the body weight of the cobia after 2-week feeding with LAB4012 was 12% higher than that of non-fed control group. Although feeding with LAB4012 did not enhance the phagocytic activity of peripheral blood leukocytes (PBLs), but the respiratory burst of PBLs was significantly elevated. Feeding with LAB4012 did not improve the specific immune response of cobia after immunization with inactivated Pdp, but significantly increased 20% survival rate after Pdp challenge. The synergetic protection is highly possible related with LAB4012-induced innate immunity. In summary, LAB4012 is suggested to be a probiotic for cobia against Pdp infection. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:18:22Z (GMT). No. of bitstreams: 1 ntu-99-R97b41023-1.pdf: 4432957 bytes, checksum: 41189f052989f446c9861999366a0196 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要................................................................................................I
Abstract...............................................................................................II Contents..............................................................................................III Index of Tables......................................................................................VI Index of Figures...................................................................................VII Index of Appendixes............................................................................VIII I. Introduction.......................................................................................1 1. Cobia......................................................................................1 1.1. Characteristics......................................................................1 1.2. Diseases..............................................................................1 2. Photobacterium damselae subsp. piscicida..........................................2 2.1. Characteristics.....................................................................2 2.2. Pathogenicity........................................................................3 2.3. Photobacteriosis.....................................................................4 3. Probiotics.................................................................................5 3.1. The definition of probiotic........................................................5 3.2. The application of probiotic in aquaculture....................................6 3.3. Characteristics of Lactic acid bacteria............................................7 4. The aim of this study....................................................................8 II. Materials and Methods......................................................................10 1. Bacteria.................................................................................10 2. Identification of LAB strains by 16S rDNA.......................................10 3. Anti-Pdp activities of the LAB strains..............................................11 4. Cobia....................................................................................11 5. Preparation of LAB-containing feed................................................12 6. Pdp challenge...........................................................................12 7. Screening of probiotica candidates.................................................13 8. The effect of feeding duration........................................................13 9. The effect of LAB on innate immune response....................................14 9.1. Phagocytosis assay...............................................................14 9.2. Respiratory burst activity........................................................15 10. The synergetic protection induced by LAB4012 on Pdp-vaccinated cobia.....16 10.1.Vaccination of cobia with inactivated Pdp.....................................16 10.2.Quantification of anti-Pdp immunoglobulin....................................17 11. Statistical analysis....................................................................18 III. Results.........................................................................................19 1. Growth rates of Pdp in the presence of LAB’s metabolites.....................19 2.Survival rates of the cobia separately fed with three LABs......................19 3. The relationship of feeding duration and protection efficacy on against Pdp infection...................................20 3. The relationship of feeding duration and protection efficacy on against Pdp infection..................................................................................20 4. Feeding with LAB4012 increased the growth rate of cobia...................20 5. The effect of LAB4012 on the innate immunity...................................20 6. Feeding with LAB4012 effectively protected cobia from Pdp infection...21 7. Feeding with LAB4012 did not improve the antibody response after vaccination.......................................................22 8. Feeding with LAB4012 induced synergetic protection in vaccinated cobia against Pdp challenge................................................................22 IV. Discussion.....................................................................................24 V. References......................................................................................30 Tables............................................................................................44 Figures...............................................................................................46 Appendixes.......................................................................................56 | |
dc.language.iso | en | |
dc.title | 餵食乳酸菌對海鱺抗發光桿菌症之保護作用 | zh_TW |
dc.title | Protection of cobia (Rachycentron canadum) from photobacteriosis by feeding lactic acid bacteria Pediococcus pentosaceus | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 胡宏熙,宋延齡,陳俊堯 | |
dc.subject.keyword | 海鱺,益生菌,乳酸菌,發光桿菌症,發光桿菌, | zh_TW |
dc.subject.keyword | Cobia,Probiotic,Lactic acid bacteria,Photobacteriosis,Photobacterium damselae subsp. piscicida, | en |
dc.relation.page | 60 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2010-07-27 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 4.33 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。