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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 許輔 | |
dc.contributor.author | Chen-Hao Yeh | en |
dc.contributor.author | 葉鎮豪 | zh_TW |
dc.date.accessioned | 2021-06-08T04:16:08Z | - |
dc.date.copyright | 2010-08-16 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-04 | |
dc.identifier.citation | References
Abadie, J. M.; Blassingame, C. L.; Bankson, D. D., Albumin cobalt binding assay to rule out acute coronary syndrome. Ann Clin Lab Sci 2005, 35 (1), 66-72. Ackerson, C. J.; Jadzinsky, P. D.; Jensen, G. J.; Kornberg, R. D., Rigid, specific, and discrete gold nanoparticle/antibody conjugates. J Am Chem Soc 2006, 128 (8), 2635-40. Adaskaveg, J. E.; Gilbertson, R. L.; Blanchette, R. A., Comparative studies of delignification caused by ganoderma species. Appl Environ Microbiol 1990, 56 (6), 1932-43. Adaskaveg, J. E.; Gilbertson, R. L.; Dunlap, M. R., Effects of Incubation Time and Temperature on In Vitro Selective Delignification of Silver Leaf Oak by Ganoderma colossum.Appl Environ Microbiol 1995, 61 (1), 138-44. Ali, O. A.; Huebsch, N.; Cao, L.; Dranoff, G.; Mooney, D. J., Infection-mimicking materials to program dendritic cells in situ. Nat Mater 2009, 8 (2), 151-8. Aslan, K.; Luhrs, C. C.; Perez-Luna, V. H., Controlled and reversible aggregation of biotinylated gold nanoparticles with streptavidin. J Phys Chem B 2004, 108 (40), 15631-15639. Aubin-Tam, M. E.; Hamad-Schifferli, K., Gold nanoparticle-cytochrome C complexes: the effect of nanoparticle ligand charge on protein structure. Langmuir 2005, 21 (26), 12080-4. Aubin-Tam, M. E.; Hamad-Schifferli, K., Structure and function of nanoparticle-protein conjugates. Biomed Mater 2008, 3 (3), 034001. Bastus, N. G.; Sanchez-Tillo, E.; Pujals, S.; Farrera, C.; Lopez, C.; Giralt, E.; Celada, A.; Lloberas, J.; Puntes, V., Homogeneous conjugation of peptides onto gold nanoparticles enhances macrophage response. ACS Nano 2009, 3 (6), 1335-44. Boisselier, E.; Astruc, D., Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem Soc Rev 2009, 38 (6), 1759-82. Bonifaz, L. C.; Bonnyay, D. P.; Charalambous, A.; Darguste, D. I.; Fujii, S.; Soares, H.; Brimnes, M. K.; Moltedo, B.; Moran, T. M.; Steinman, R. M., In vivo targeting of antigens to maturing dendritic cells via the DEC-205 receptor improves T cell vaccination. J Exp Med 2004, 199 (6), 815-24. Cao, L. Z.; Lin, Z. B., Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides. Immunol Lett 2002, 83 (3), 163-9. Chang, H. H.; Yeh, C. H.; Sheu, F., A novel immunomodulatory protein from Poria cocos induces Toll-like receptor 4-dependent activation within mouse peritoneal macrophages.J Agric Food Chem 2009, 57 (14), 6129-39. Chen, W. C.; Hau, D. M.; Wang, C. C.; Lin, I. H.; Lee, S. S., Effects of Ganoderma lucidum and krestin on subset T-cell in spleen of gamma-irradiated mice. Am J Chin Med 1995,23 (3-4), 289-98. Chien, C. M.; Cheng, J. L.; Chang, W. T.; Tien, M. H.; Tsao, C. M.; Chang, Y. H.; Chang, H. Y.; Hsieh, J. F.; Wong, C. H.; Chen, S. T., Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56+ NK-cell cytotoxicity in cord blood. Bioorg Med Chem 2004, 12 (21), 5603-9. Chieppa, M.; Bianchi, G.; Doni, A.; Del Prete, A.; Sironi, M.; Laskarin, G.; Monti, P.; Piemonti, L.; Biondi, A.; Mantovani, A.; Introna, M.; Allavena, P., Cross-linking of the mannose receptor on monocyte-derived dendritic cells activates an anti-inflammatory immunosuppressive program. J Immunol 2003, 171 (9), 4552-60. Chithrani, B. D.; Ghazani, A. A.; Chan, W. C., Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett 2006, 6 (4), 662-8. Chow, M. K.; Zukoski, C. F., Gold Sol Formation Mechanisms - Role of Colloidal Stability. J Colloid Interf Sci 1994, 165 (1), 97-109. Cognet, L.; Tardin, C.; Boyer, D.; Choquet, D.; Tamarat, P.; Lounis, B., Single metallic nanoparticle imaging for protein detection in cells. Proc Natl Acad Sci U S A 2003, 100(20), 11350-5. Cone, R. A., Barrier properties of mucus. Adv Drug Deliv Rev 2009, 61 (2), 75-85. Corot, C.; Petry, K. G.; Trivedi, R.; Saleh, A.; Jonkmanns, C.; Le Bas, J. F.; Blezer, E.; Rausch, M.; Brochet, B.; Foster-Gareau, P.; Baleriaux, D.; Gaillard, S.; Dousset, V., Macrophage imaging in central nervous system and in carotid atherosclerotic plaque using ultrasmall superparamagnetic iron oxide in magnetic resonance imaging. Invest Radiol2004, 39 (10), 619-25. Cosmi, L.; Liotta, F.; Lazzeri, E.; Francalanci, M.; Angeli, R.; Mazzinghi, B.; Santarlasci, V.; Manetti, R.; Vanini, V.; Romagnani, P.; Maggi, E.; Romagnani, S.; Annunziato, F., Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood 2003, 102 (12), 4107-14. Cushing, B. L.; Kolesnichenko, V. L.; O'Connor, C. J., Recent advances in the liquid-phase syntheses of inorganic nanoparticles. Chem Rev 2004, 104 (9), 3893-946. Cyster, J. G., Chemokines and cell migration in secondary lymphoid organs. Science 1999, 286 (5447), 2098-102. Daniel, M. C.; Astruc, D., Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 2004, 104 (1), 293-346. de la Fuente, J. M.; Berry, C. C., Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus. Bioconjug Chem 2005, 16 (5), 1176-80. Debouttiere, P. J.; Roux, S.; Vocanson, F.; Billotey, C.; Beuf, O.; Favre-Reguillon, A.; Lin, Y.; Pellet-Rostaing, S.; Lamartine, R.; Perriat, P.; Tillement, O., Design of gold nanoparticles for magnetic resonance imaging. Adv Funct Mater 2006, 16 (18), 2330-2339. Dixit, V.; Van den Bossche, J.; Sherman, D. M.; Thompson, D. H.; Andres, R. P., Synthesis and grafting of thioctic acid-PEG-folate conjugates onto Au nanoparticles for selective targeting of folate receptor-positive tumor cells. Bioconjug Chem 2006, 17 (3), 603-9. Dubois, P. M.; Stepinski, J.; Urbain, J.; Sibley, C. H., Role of the transmembrane and cytoplasmic domains of surface IgM in endocytosis and signal transduction. Eur J Immunol1992, 22 (3), 851-7. Eisenbarth, S. C.; Colegio, O. R.; O'Connor, W.; Sutterwala, F. S.; Flavell, R. A., Crucial role for the Nalp3 inflammasome in the immunostimulatory properties of aluminium adjuvants. Nature 2008, 453 (7198), 1122-6. el-Mekkawy, S.; Meselhy, M. R.; Nakamura, N.; Tezuka, Y.; Hattori, M.; Kakiuchi, N.; Shimotohno, K.; Kawahata, T.; Otake, T., Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum. Phytochemistry 1998, 49 (6), 1651-7. Eo, S. K.; Kim, Y. S.; Lee, C. K.; Han, S. S., Antiherpetic activities of various protein bound polysaccharides isolated from Ganoderma lucidum. J Ethnopharmacol 1999, 68 (1-3), 175-81. Eo, S. K.; Kim, Y. S.; Lee, C. K.; Han, S. S., Antiviral activities of various water and methanol soluble substances isolated from Ganoderma lucidum. J Ethnopharmacol 1999, 68(1-3), 129-36. Eo, S. K.; Kim, Y. S.; Lee, C. K.; Han, S. S., Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses. J Ethnopharmacol 2000, 72 (3), 475-81. Fahmy, T. M.; Bieler, J. G.; Edidin, M.; Schneck, J. P., Increased TCR avidity after T cell activation: a mechanism for sensing low-density antigen. Immunity 2001, 14 (2), 135-43. Fanning, S. L.; George, T. C.; Feng, D.; Feldman, S. B.; Megjugorac, N. J.; Izaguirre, A. G.; Fitzgerald-Bocarsly, P., Receptor cross-linking on human plasmacytoid dendritic cells leads to the regulation of IFN-alpha production. J Immunol 2006, 177 (9), 5829-39. Faulk, W. P.; Taylor, G. M., An immunocolloid method for the electron microscope. Immunochemistry 1971, 8 (11), 1081-3. Felsenfeld, D. P.; Choquet, D.; Sheetz, M. P., Ligand binding regulates the directed movement of beta1 integrins on fibroblasts. Nature 1996, 383 (6599), 438-40. Frens, J., A device for quantative measuring of shivering in goats. Lab Anim 1973, 7 (3), 287-8. Freund, P. L.; Spiro, M., Colloidal Catalysis - the Effect of Sol Size and Concentration. J Phys Chem-Us 1985, 89 (7), 1074-1077. Gao, Y.; Zhou, S.; Jiang, W.; Huang, M.; Dai, X., Effects of ganopoly (a Ganoderma lucidum polysaccharide extract) on the immune functions in advanced-stage cancer patients.Immunol Invest 2003, 32 (3), 201-15. Geoghegan, W. D.; Ackerman, G. A., Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem 1977,25 (11), 1187-200. Georganopoulou, D. G.; Chang, L.; Nam, J. M.; Thaxton, C. S.; Mufson, E. J.; Klein, W. L.; Mirkin, C. A., Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease. P Natl Acad Sci USA 2005, 102 (7), 2273-2276. Graham, V. A.; Marzo, A. L.; Tough, D. F., A role for CD44 in T cell development and function during direct competition between CD44+ and CD44- cells. Eur J Immunol 2007,37 (4), 925-34. Grewal, I. S.; Flavell, R. A., CD40 and CD154 in cell-mediated immunity. Annu Rev Immunol 1998, 16, 111-35. Guarise, C.; Pasquato, L.; De Filippis, V.; Scrimin, P., Gold nanoparticles-based protease assay. P Natl Acad Sci USA 2006, 103 (11), 3978-3982. Haak-Frendscho, M.; Kino, K.; Sone, T.; Jardieu, P., Ling Zhi-8: a novel T cell mitogen induces cytokine production and upregulation of ICAM-1 expression. Cell Immunol 1993,150 (1), 101-13. Hainfeld, J. F.; Liu, W.; Halsey, C. M.; Freimuth, P.; Powell, R. D., Ni-NTA-gold clusters target His-tagged proteins. J Struct Biol 1999, 127 (2), 185-98. Hainfeld, J. F.; Slatkin, D. N.; Focella, T. M.; Smilowitz, H. M., Gold nanoparticles: a new X-ray contrast agent. Br J Radiol 2006, 79 (939), 248-53. He, C. Y.; Li, W. D.; Guo, S. X.; Lin, S. Q.; Lin, Z. B., Effect of polysaccharides from Ganoderma lucidum on streptozotocin-induced diabetic nephropathy in mice. J Asian Nat Prod Res 2006, 8 (8), 705-11. Herdt, A. R.; Drawz, S. M.; Kang, Y. J.; Taton, T. A., DNA dissociation and degradation at gold nanoparticle surfaces. Colloid Surface B 2006, 51 (2), 130-139. Hikino, H.; Ishiyama, M.; Suzuki, Y.; Konno, C., Mechanisms of hypoglycemic activity of ganoderan B: a glycan of Ganoderma lucidum fruit bodies. Planta Med 1989, 55 (5), 423-8. Hikino, H.; Konno, C.; Mirin, Y.; Hayashi, T., Isolation and Hypoglycemic Activity of Ganoderans A and B, Glycans of Ganoderma lucidum Fruit Bodies1. Planta Med 1985, 51 (4), 339-40. Hirsch, L. R.; Jackson, J. B.; Lee, A.; Halas, N. J.; West, J. L., A whole blood immunoassay using gold nanoshells. Anal Chem 2003, 75 (10), 2377-81. Hori, Y.; Winans, A. M.; Huang, C. C.; Horrigan, E. M.; Irvine, D. J., Injectable dendritic cell-carrying alginate gels for immunization and immunotherapy. Biomaterials 2008, 29 (27), 3671-82. Horisberger, M.; Vauthey, M., Labelling of colloidal gold with protein. A quantitative study using beta-lactoglobulin. Histochemistry 1984, 80 (1), 13-8. Hsiao, J. K.; Chu, H. H.; Wang, Y. H.; Lai, C. W.; Chou, P. T.; Hsieh, S. T.; Wang, J. L.; Liu, H. M., Macrophage physiological function after superparamagnetic iron oxide labeling.NMR Biomed 2008, 21 (8), 820-9. Hsu, H. Y.; Hua, K. F.; Lin, C. C.; Lin, C. H.; Hsu, J.; Wong, C. H., Extract of Reishi polysaccharides induces cytokine expression via TLR4-modulated protein kinase signaling pathways. J Immunol 2004, 173 (10), 5989-99. Hsu, H. Y.; Hua, K. F.; Wu, W. C.; Hsu, J.; Weng, S. T.; Lin, T. L.; Liu, C. Y.; Hseu, R. S.; Huang, C. T., Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells. J Cell Physiol 2008, 215 (1), 15-26. Hsu, M. J.; Lee, S. S.; Lee, S. T.; Lin, W. W., Signaling mechanisms of enhanced neutrophil phagocytosis and chemotaxis by the polysaccharide purified from Ganoderma lucidum. Br J Pharmacol 2003, 139 (2), 289-98. Hsu, M. J.; Lee, S. S.; Lin, W. W., Polysaccharide purified from Ganoderma lucidum inhibits spontaneous and Fas-mediated apoptosis in human neutrophils through activation of the phosphatidylinositol 3 kinase/Akt signaling pathway. J Leukoc Biol 2002, 72 (1), 207-16. Hu, H.; Ahn, N. S.; Yang, X.; Lee, Y. S.; Kang, K. S., Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cell. Int J Cancer 2002,102 (3), 250-3. Huang, L.; Sun, F.; Liang, C. Y.; He, Y. X.; Bao, R.; Liu, L. X.; Zhou, C. Z., Crystal structure of LZ-8 from the medicinal fungus Ganoderma lucidium. Proteins-Structure Function and Bioinformatics 2009, 75 (2), 524-527. Huang, X.; Jain, P. K.; El-Sayed, I. H.; El-Sayed, M. A., Determination of the minimum temperature required for selective photothermal destruction of cancer cells with the use of immunotargeted gold nanoparticles. Photochem Photobiol 2006, 82 (2), 412-7. Hubbell, J. A.; Thomas, S. N.; Swartz, M. A., Materials engineering for immunomodulation. Nature 2009, 462 (7272), 449-60. Huie, C. W.; Di, X., Chromatographic and electrophoretic methods for Lingzhi pharmacologically active components. J Chromatogr B Analyt Technol Biomed Life Sci 2004, 812(1-2), 241-57. Hwang, S. F.; Liu, K. J.; Kuan, Y. H.; Tung, K. S.; Su, C. H.; Tung, T. C., The inhibitory effect on artificial pulmonary metastasis of murine S-180 sarcoma cells by orally administered Ganoderma lucidum culture broth. J. Chinese Oncol. Soc. 1989, 5, 10-15. Jiang, W.; Kim, B. Y.; Rutka, J. T.; Chan, W. C., Nanoparticle-mediated cellular response is size-dependent. Nat Nanotechnol 2008, 3 (3), 145-50. Jiang, W.; Swiggard, W. J.; Heufler, C.; Peng, M.; Mirza, A.; Steinman, R. M.; Nussenzweig, M. C., The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature 1995, 375 (6527), 151-5. Kanaras, A. G.; Kamounah, F. S.; Schaumburg, K.; Kiely, C. J.; Brust, M., Thioalkylated tetraethylene glycol: a new ligand for water soluble monolayer protected gold clusters.Chem Commun (Camb) 2002, (20), 2294-5. Kappes, D. J.; He, X., Role of the transcription factor Th-POK in CD4:CD8 lineage commitment. Immunol Rev 2006, 209, 237-52. Kapsenberg, M. L., Dendritic-cell control of pathogen-driven T-cell polarization. Nat Rev Immunol 2003, 3 (12), 984-93. Kelly, K. L.; Coronado, E.; Zhao, L. L.; Schatz, G. C., The optical properties of metal nanoparticles: The influence of size, shape, and dielectric environment. J Phys Chem B 2003,107 (3), 668-677. Kiessling, L. L.; Gestwicki, J. E.; Strong, L. E., Synthetic multivalent ligands as probes of signal transduction. Angew Chem Int Ed Engl 2006, 45 (15), 2348-68. Kino, K.; Yamashita, A.; Yamaoka, K.; Watanabe, J.; Tanaka, S.; Ko, K.; Shimizu, K.; Tsunoo, H., Isolation and characterization of a new immunomodulatory protein, ling zhi-8 (LZ-8), from Ganoderma lucidium. J Biol Chem 1989, 264 (1), 472-8. Kleindienst, P.; Brocker, T., Endogenous dendritic cells are required for amplification of T cell responses induced by dendritic cell vaccines in vivo. J Immunol 2003, 170 (6), 2817-23. Ko, J. L.; Lin, S. J.; Hsu, C. I.; Kao, C. L.; Lin, J. Y., Molecular cloning and expression of a fungal immunomodulatory protein, FIP-fve, from Flammulina velutipes. J Formos Med Assoc 1997, 96 (7), 517-24. Koiwai, K.; Tokuhisa, K.; Karinaga, R.; Kudo, Y.; Kusuki, S.; Takeda, Y.; Sakurai, K., Transition from a normal to inverted cylinder for an amidine-bearing lipid/pDNA complex and its excellent transfection. Bioconjug Chem 2005, 16 (6), 1349-51. Kumar, S.; Gandhi, K. S.; Kumar, R., Modeling of formation of gold nanoparticles by citrate method. Ind Eng Chem Res 2007, 46 (10), 3128-3136. Kuriyama, S.; Mitoro, A.; Tsujinoue, H.; Nakatani, T.; Yoshiji, H.; Tsujimoto, T.; Yamazaki, M.; Fukui, H., Particle-mediated gene transfer into murine livers using a newly developed gene gun. Gene Ther 2000, 7 (13), 1132-6. 80. Kwon, Y. J.; James, E.; Shastri, N.; Frechet, J. M., In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles. Proc Natl Acad Sci U S A 2005, 102 (51), 18264-8. Lakshmi, B.; Ajith, T. A.; Sheena, N.; Gunapalan, N.; Janardhanan, K. K., Antiperoxidative, anti-inflammatory, and antimutagenic activities of ethanol extract of the mycelium of Ganoderma lucidum occurring in South India. Teratog Carcinog Mutagen 2003, Suppl 1, 85-97. Lakshmi, B.; Ajith, T. A.; Sheena, N.; Gunapalan, N.; Janardhanan, K. K., Antiperoxidative, anti-inflammatory, and antimutagenic activities of ethanol extract of the mycelium of Ganoderma lucidum occurring in South India. Teratog Carcinog Mutagen 2003, Suppl 1, 85-97. Lasne, D.; Blab, G. A.; Berciaud, S.; Heine, M.; Groc, L.; Choquet, D.; Cognet, L.; Lounis, B., Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cells.Biophys J 2006, 91 (12), 4598-604. Lee, M. S.; Kim, Y. J., Signaling pathways downstream of pattern-recognition receptors and their cross talk. Annu Rev Biochem 2007, 76, 447-80. Li, H.; Nookala, S.; Re, F., Aluminum hydroxide adjuvants activate caspase-1 and induce IL-1beta and IL-18 release. J Immunol 2007, 178 (8), 5271-6. Li, H.; Willingham, S. B.; Ting, J. P.; Re, F., Cutting edge: inflammasome activation by alum and alum's adjuvant effect are mediated by NLRP3. J Immunol 2008, 181 (1), 17-21. Li, Y.; Hahn, D.; Holzgreve, W.; Hahn, S., Ready detection of donor-specific single-nucleotide polymorphisms in the urine of renal transplant recipients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Chem 2005, 51 (10), 1903-4. Li, Z.; Liu, J.; Zhao, Y., Possible mechanism underlying the antiherpetic activity of a proteoglycan isolated from the mycelia of Ganoderma lucidum in vitro. J Biochem Mol Biol2005, 38 (1), 34-40. Lim, Y. T.; Cho, M. Y.; Choi, B. S.; Noh, Y. W.; Chung, B. H., Diagnosis and therapy of macrophage cells using dextran-coated near-infrared responsive hollow-type gold nanoparticles. Nanotechnology 2008, 19 (37), -. Lin, K. I.; Kao, Y. Y.; Kuo, H. K.; Yang, W. B.; Chou, A.; Lin, H. H.; Yu, A. L.; Wong, C. H., Reishi polysaccharides induce immunoglobulin production through the TLR4/TLR2-mediated induction of transcription factor Blimp-1. J Biol Chem 2006, 281 (34), 24111-23. Lin, S. B.; Li, C. H.; Lee, S. S.; Kan, L. S., Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C, activating mitogen-activated protein kinases and G2-phase cell cycle arrest. Life Sci 2003, 72 (21), 2381-90. Lin, W. H.; Hung, C. H.; Hsu, C. I.; Lin, J. Y., Dimerization of the N-terminal amphipathic alpha-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis. J Biol Chem 1997, 272 (32), 20044-8. Lin, Y. L.; Liang, Y. C.; Lee, S. S.; Chiang, B. L., Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and p38 mitogen-activated protein kinase pathways. J Leukoc Biol 2005, 78 (2), 533-43. Lin, Y. L.; Liang, Y. C.; Tseng, Y. S.; Huang, H. Y.; Chou, S. Y.; Hseu, R. S.; Huang, C. T.; Chiang, B. L., An immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and MAPK pathways. J Leukoc Biol 2009, 86 (4), 877-89. Lin, Z. B.; Zhang, H. N., Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms. Acta Pharmacol Sin 2004, 25 (11), 1387-95. Link, S.; El-Sayed, M. A., Size and temperature dependence of the plasmon absorption of colloidal gold nanoparticles. J Phys Chem B 1999, 103 (21), 4212-4217. Liz-Marzan, L. M., Tailoring surface plasmons through the morphology and assembly of metal nanoparticles. Langmuir 2006, 22 (1), 32-41. Loo, C.; Lowery, A.; Halas, N.; West, J.; Drezek, R., Immunotargeted nanoshells for integrated cancer imaging and therapy. Nano Lett 2005, 5 (4), 709-11. Macdonald, T. T.; Monteleone, G., Immunity, inflammation, and allergy in the gut. Science 2005, 307 (5717), 1920-5. Mahmoud, K. A.; Luong, J. H., Impedance method for detecting HIV-1 protease and screening for its inhibitors using ferrocene-peptide conjugate/Au nanoparticle/single-walled carbon nanotube modified electrode. Anal Chem 2008, 80 (18), 7056-62. Malyala, P.; Chesko, J.; Ugozzoli, M.; Goodsell, A.; Zhou, F.; Vajdy, M.; O'Hagan, D. T.; Singh, M., The potency of the adjuvant, CpG oligos, is enhanced by encapsulation in PLG microparticles. J Pharm Sci 2008, 97 (3), 1155-64. Mantovani, A.; Sica, A.; Sozzani, S.; Allavena, P.; Vecchi, A.; Locati, M., The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol2004, 25 (12), 677-86. Meylan, E.; Tschopp, J.; Karin, M., Intracellular pattern recognition receptors in the host response. Nature 2006, 442 (7098), 39-44. Miyasaka, N.; Inoue, H.; Totsuka, T.; Koike, R.; Kino, K.; Tsunoo, H., An immunomodulatory protein, Ling Zhi-8, facilitates cellular interaction through modulation of adhesion molecules. Biochem Biophys Res Commun 1992, 186 (1), 385-90. Moghimi, S. M.; Hunter, A. C.; Murray, J. C., Nanomedicine: current status and future prospects. FASEB J 2005, 19 (3), 311-30. Moncalvo, J. M.; Lutzoni, F. M.; Rehner, S. A.; Johnson, J.; Vilgalys, R., Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences.Syst Biol 2000, 49 (2), 278-305. Murasugi, A.; Tanaka, S.; Komiyama, N.; Iwata, N.; Kino, K.; Tsunoo, H.; Sakuma, S., Molecular cloning of a cDNA and a gene encoding an immunomodulatory protein, Ling Zhi-8, from a fungus, Ganoderma lucidum. J Biol Chem 1991, 266 (4), 2486-93. Nath, N.; Chilkoti, A., Interfacial phase transition of an environmentally responsive elastin biopolymer adsorbed on functionalized gold nanoparticles studied by colloidal surface plasmon resonance. Journal of the American Chemical Society 2001, 123 (34), 8197-8202. Ofodile, L. N.; Uma, N. U.; Kokubun, T.; Grayer, R. J.; Ogundipe, O. T.; Simmonds, M. S., Antimicrobial activity of some Ganoderma species from Nigeria. Phytother Res 2005,19 (4), 310-3. Ou, K.; Liu, Y.; Zhang, L.; Yang, X.; Huang, Z.; Nout, M. J.; Liang, J., Effect of neutrase, alcalase, and papain hydrolysis of whey protein concentrates on iron uptake by Caco-2 cells. J Agric Food Chem 2010, 58 (8), 4894-900. Pawelczyk, E.; Arbab, A. S.; Pandit, S.; Hu, E.; Frank, J. A., Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging. NMR Biomed 2006, 19 (5), 581-92. Quereshi, U. A.; Bhat, J. I.; Ali, S. W.; Mir, A. A.; Kambay, A. H.; Bhat, I. N., Acute salt poisoning due to different oral rehydration solution (ORS) packet sizes. Indian J Pediatr2010, 77 (6), 679-80. Reddy, S. T.; van der Vlies, A. J.; Simeoni, E.; Angeli, V.; Randolph, G. J.; O'Neil, C. P.; Lee, L. K.; Swartz, M. A.; Hubbell, J. A., Exploiting lymphatic transport and complement activation in nanoparticle vaccines. Nat Biotechnol 2007, 25 (10), 1159-64. Sakaguchi, S., Regulatory T cells: key controllers of immunologic self-tolerance. Cell 2000, 101 (5), 455-8. Scott-Browne, J. P.; White, J.; Kappler, J. W.; Gapin, L.; Marrack, P., Germline-encoded amino acids in the alphabeta T-cell receptor control thymic selection. Nature 2009, 458(7241), 1043-6. Seong, S. Y.; Matzinger, P., Hydrophobicity: an ancient damage-associated molecular pattern that initiates innate immune responses. Nat Rev Immunol 2004, 4 (6), 469-78. Seto, S. W.; Lam, T. Y.; Tam, H. L.; Au, A. L.; Chan, S. W.; Wu, J. H.; Yu, P. H.; Leung, G. P.; Ngai, S. M.; Yeung, J. H.; Leung, P. S.; Lee, S. M.; Kwan, Y. W., Novel hypoglycemic effects of Ganoderma lucidum water-extract in obese/diabetic (+db/+db) mice. Phytomedicine 2009, 16 (5), 426-36. Sharp, F. A.; Ruane, D.; Claass, B.; Creagh, E.; Harris, J.; Malyala, P.; Singh, M.; O'Hagan, D. T.; Petrilli, V.; Tschopp, J.; O'Neill, L. A.; Lavelle, E. C., Uptake of particulate vaccine adjuvants by dendritic cells activates the NALP3 inflammasome. Proc Natl Acad Sci U S A 2009, 106 (3), 870-5. Skala, M. C.; Crow, M. J.; Wax, A.; Izatt, J. A., Photothermal Optical Coherence Tomography of Epidermal Growth Factor Receptor in Live Cells Using Immunotargeted Gold Nanospheres. Nano Lett 2008, 8 (10), 3461-3467. Slifka, M. K.; Whitton, J. L., Functional avidity maturation of CD8(+) T cells without selection of higher affinity TCR. Nat Immunol 2001, 2 (8), 711-7. Sliva, D.; Labarrere, C.; Slivova, V.; Sedlak, M.; Lloyd, F. P., Jr.; Ho, N. W., Ganoderma lucidum suppresses motility of highly invasive breast and prostate cancer cells. Biochem Biophys Res Commun 2002, 298 (4), 603-12. Sokolov, K.; Follen, M.; Aaron, J.; Pavlova, I.; Malpica, A.; Lotan, R.; Richards-Kortum, R., Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles. Cancer Research 2003, 63 (9), 1999-2004. Sperling, R. A.; Rivera Gil, P.; Zhang, F.; Zanella, M.; Parak, W. J., Biological applications of gold nanoparticles. Chem Soc Rev 2008, 37 (9), 1896-908. Steinman, R. M.; Banchereau, J., Taking dendritic cells into medicine. Nature 2007, 449 (7161), 419-26. Stern, J. M.; Stanfield, J.; Kabbani, W.; Hsieh, J. T.; Cadeddu, J. A., Selective prostate cancer thermal ablation with laser activated gold nanoshells. J Urol 2008, 179 (2), 748-53. Storhoff, J. J.; Lazarides, A. A.; Mucic, R. C.; Mirkin, C. A.; Letsinger, R. L.; Schatz, G. C., What controls the optical properties of DNA-linked gold nanoparticle assemblies?Journal of the American Chemical Society 2000, 122 (19), 4640-4650. Sun, J.; He, H.; Xie, B. J., Novel antioxidant peptides from fermented mushroom Ganoderma lucidum. J Agric Food Chem 2004, 52 (21), 6646-52. Tanaka, S.; Ko, K.; Kino, K.; Tsuchiya, K.; Yamashita, A.; Murasugi, A.; Sakuma, S.; Tsunoo, H., Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8). An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions. J Biol Chem 1989, 264 (28), 16372-7. Taylor, P. R.; Martinez-Pomares, L.; Stacey, M.; Lin, H. H.; Brown, G. D.; Gordon, S., Macrophage receptors and immune recognition. Annu Rev Immunol 2005, 23, 901-44. Tong, M. H.; Chien, P. J.; Chang, H. H.; Tsai, M. J.; Sheu, F., High processing tolerances of immunomodulatory proteins in Enoki and Reishi mushrooms. J Agric Food Chem2008, 56 (9), 3160-6. van der Hem, L. G.; van der Vliet, J. A.; Bocken, C. F.; Kino, K.; Hoitsma, A. J.; Tax, W. J., Ling Zhi-8: studies of a new immunomodulating agent. Transplantation 1995, 60 (5), 438-43. Verma, A.; Uzun, O.; Hu, Y.; Han, H. S.; Watson, N.; Chen, S.; Irvine, D. J.; Stellacci, F., Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. Nat Mater 2008, 7 (7), 588-95. Wang, S. Y.; Hsu, M. L.; Hsu, H. C.; Tzeng, C. H.; Lee, S. S.; Shiao, M. S.; Ho, C. K., The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int J Cancer 1997, 70 (6), 699-705. Wang, X.; Yuan, Y.; Wang, K.; Zhang, D.; Yang, Z.; Xu, P., Deproteinization of gellan gum produced by Sphingomonas paucimobilis ATCC 31461. Journal of Biotechnology2007, 128 (2), 403-7. Wang, Y. Y.; Khoo, K. H.; Chen, S. T.; Lin, C. C.; Wong, C. H.; Lin, C. H., Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorg Med Chem 2002, 10 (4), 1057-62. Xue, Q.; Ding, Y.; Shang, C.; Jiang, C.; Zhao, M., Functional expression of LZ-8, a fungal immunomodulatory protein from Ganoderma lucidium in Pichia pastoris. J Gen Appl Microbiol 2008, 54 (6), 393-8. Yang, P. H.; Sun, X.; Chiu, J. F.; Sun, H.; He, Q. Y., Transferrin-mediated gold nanoparticle cellular uptake. Bioconjug Chem 2005, 16 (3), 494-6. Yeh, C. H.; Hsiao, J. K.; Wang, J. L.; Sheu, F., Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages. J Nanopart Res 2010, 12(1), 151-160. Yeh, C. M.; Yeh, C. K.; Hsu, X. Y.; Luo, Q. M.; Lin, M. Y., Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis. Appl Environ Microbiol 2008, 74 (4), 1039-49. Yokoyama, H.; Dutriez, C.; Li, L.; Nemoto, T.; Sugiyama, K.; Sasaki, S.; Masunaga, H.; Takata, M.; Okuda, H., Grazing incident small angle x-ray scattering study of polymer thin films with embedded ordered nanometer cells. J Chem Phys 2007, 127 (1), -. Zayat, L.; Salierno, M.; Etchenique, R., Ruthenium(II) bipyridyl complexes as photolabile caging groups for amines. Inorg Chem 2006, 45 (4), 1728-31. Zayats, A. V.; Smolyaninov, II, High-optical-throughput individual nanoscale aperture in a multilayered metallic film. Opt Lett 2006, 31 (3), 398-400. Zayats, M.; Huang, Y.; Gill, R.; Ma, C. A.; Willner, I., Label-free and reagentless aptamer-based sensors for small molecules. J Am Chem Soc 2006, 128 (42), 13666-7. Zhang, C. X.; Zhang, Y.; Wang, X.; Tang, Z. M.; Lu, Z. H., Hyper-Rayleigh scattering of protein-modified gold nanoparticles. Anal Biochem 2003, 320 (1), 136-40. Zhang, H. N.; He, J. H.; Yuan, L.; Lin, Z. B., In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life Sci2003, 73 (18), 2307-19. Zhao, X.; Jain, S.; Benjamin Larman, H.; Gonzalez, S.; Irvine, D. J., Directed cell migration via chemoattractants released from degradable microspheres. Biomaterials 2005, 26(24), 5048-63. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22357 | - |
dc.description.abstract | 園產品功能性成分進行奈米化處理,根據其粒徑、形狀以及材料性質而在調控功能性成分的消化吸收與生物可利用性上具有不同的利用價值。然而,就分子層次而言,粒徑不同之奈米化園產品與細胞間之交互作用仍屬未知。靈芝菌絲體中萃取出之免疫調節蛋白LZ-8 (Ling Zhi-8),經酵母菌重組後以單體之形式存在,其分子量約為15 kDa。本研究中,利用靜電吸附原理 (electrostatic adsorption) 將LZ-8鍵結構築於奈米金粒子 (gold nanoparticles) 上,獲得大小分布於10奈米至100奈米的穩定LZ-8奈米金粒子,其LZ-8蛋白的鍵結量與奈米金粒子之大小成正比。
LZ-8奈米金粒子處理小鼠腹腔巨噬細胞可以提升LZ-8刺激巨噬細胞產生IL-1β與IL-10之能力,而流式細胞儀分析經GNP-LZ-8刺激之小鼠巨噬細胞,則顯示多價構築LZ-8於奈米金粒子上,可以提升LZ-8刺激細胞表面分子MHC class II, CD80, CD86的表現量增加,其中以30nm以上GNP-LZ-8的效果較為顯著。巨噬細胞的顆粒性增加則與奈米金粒子之大小成正相關。與同濃度的LZ-8測試組比較,LZ-8奈米金粒子處理小鼠脾細胞可以明顯提升脾細胞增生與刺激IL-2、INF-γ細胞激素分泌之能力,其中以50nm與30nm大小的GNP-LZ-8效果最為顯著;流式細胞儀分析經GNP-LZ-8刺激之小鼠脾細胞,顯示構築LZ-8於奈米金粒子上,可以提升LZ-8刺激CD3+與CD3-細胞表面CD25分子表現量增加與顆粒性上升,其中同樣以50與30nm的LZ-8奈米金粒子效果較佳。上述結果顯示,LZ-8奈米金粒子所引發的免疫調節活性在吞噬與非吞噬細胞中具有不同的規模依賴性。而奈米化處理除考量其生物傳遞特性外,其粒徑之大小,亦會影響LZ-8之生物活性,此一發現也許對奈米技術在園產品上的應用與園產品功能性之探討有所助益。 | zh_TW |
dc.description.abstract | Nano-scaled functional ingredients with different sizes, shapes and material properties have many applications in biological transport and hence the bioavailability. In spite of what has been achieved so far, cellular response between nano-scaled functional ingredient and target cells remains mostly unknown. Immunomodulatory protein named Ling Zhi-8 (LZ-8) extracted from the mycelia of Ganoderma lucidum has been cloned and expressed at Saccharomyces cerevisiae expression system as a recombinant protein rLZ-8. In the present study, suspensions of gold nanoparticles (GNPs) with stable surface rLZ-8 coating were prepared using electrostatic adsorption strategy. As size distribution from 10 to 100 nm, the quantity of rLZ-8 binding on GNPs was obviously correlated with particle size, which significantly enhanced the multivalency of rLZ-8 being conjugated on GNP surface.
Treatment of mouse peritoneal macrophages with GNP-LZ-8 significantly increased IL-1β cytokine production and surface CD80/86, MHC class II molecule expression as compare with the univalent free form LZ-8. Although the induction of cytokine and surface molecule activities was enhanced for all GNP-LZ-8, particle with larger size exhibited greater difference on both cytokine production and surface costimulatroy molecule expression on macrophages. Furthermore, side scatter (SSC) variation of macrophages after GNP-LZ-8 uptake revealing a granularity enhancement as well as an increase of GNP-LZ-8 size. GNP-LZ-8 treatment further indicated the enhancement of cell proliferation, IL-2 and INF-γ cytokine production as compare to univalent free form rLZ-8 on splenoctes. Moreover, free form rLZ-8 could activate the surface expression of CD25 on CD3+ splenocytes, while GNP-LZ-8 treatment elicited more significant upregulation on both CD3+ and CD3- cells. Among splenocyte treated with various sizes GNP-LZ-8, the greatest difference was observed in 30 and 50 nm GNP-LZ-8-treated cells. Taken together, these data implied the induction of diverse size-dependent immunomodulatory responses between phagocyte and non-phagocyte. Base on these results, nano-size modification should no longer be consider only as a passive rule of delivery or bio-transportation, but could also play an active role in mediating biological effects. The findings presented here may assist in the design and development of nano-scaled functional ingredient. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:16:08Z (GMT). No. of bitstreams: 1 ntu-99-R97628209-1.pdf: 8717609 bytes, checksum: 11d2f418c60035b2df61139752d54286 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | Content
誌謝....................................................................................................................................I 摘要...................................................................................................................................II Abstract............................................................................................................................III Content..............................................................................................................................V Chapter 1. Introduction..................................................................................................1 1.1. Preface.................................................................................................................1 1.2. Overview of Ganoderma lucidum.......................................................................3 1.2.1. Classification and morphological characteristics of G. lucidum...............3 1.2.2. Bioactivities of chemical components from G. lucidum...........................5 1.2.2.1. Immunomodulatory activity............................................................5 1.2.2.2. Anti-bacterial activity......................................................................7 1.2.2.3. Anti-viral activity.............................................................................8 1.2.2.4. Hypoglycemic activity.....................................................................9 1.2.2.5. Anti-tumor activity........................................................................10 1.3. Fungal immunomodulatory protein Ling Zhi-8................................................11 1.3.1. Physical and molecular property of LZ-8...............................................11 1.3.2. Heterologous expression of LZ-8...........................................................13 1.3.3. Immunomodulatory activity of LZ-8......................................................14 1.4. Materials engineering for immunomodulation..................................................15 1.4.1. Materials engineering for enhancing antigen uptake by APC.................16 1.4.2. Materials for intracellular targeting.........................................................19 1.4.3. Materials to trigger immune-specific functions.......................................20 1.5. Overview of Gold nanoparticles.........................................................................23 1.5.1. Synthesis of gold nanoparticles...............................................................23 1.5.2. Surface modification and protein-conjugation of GNPs..........................25 1.5.3. Application of gold nanoparticles in biology and medicine....................28 1.5.3.1. Application of gold nanoparticles in labeling and imaging...........28 1.5.3.2. Application of gold nanoparticles as a vehicle for delivery..........30 1.5.3.3. Application of gold nanoparticles in detection..............................31 1.5.3.4. Application of gold nanoparticles in diagnostics and therapies....34 Chapter 2. Materials and methods...............................................................................36 2.1. Materials............................................................................................................36 2.1.1. Animals....................................................................................................36 2.1.2. Reagents and chemicals...........................................................................36 2.1.3. Commercial kits.......................................................................................38 2.1.4. Instruments..............................................................................................38 2.2. Methods..............................................................................................................39 2.2.1 Functional expression and detection of rLZ-8..........................................39 2.2.1.1. Quantitative expression of rLZ-8 in Saccharomyces cerevisiae.39 2.2.1.2. LZ-8 specific monoclonal antibody production and western blotting.....................................................................................................41 2.2.2. Synthesis of rLZ-8 conjugated gold nanoparticles..................................42 2.2.2.1. Preperation of gold nanoparticles with different sizes................42 2.2.2.2. rLZ-8 protein adsorption on gold nanoparticles.........................43 2.2.2.2.1. Optimal conjugation pH adjustment and protein concentration of GNP-LZ-8...........................................43 2.2.2.2.2. Quantitative production of Gold Nanoparticle conjugates with LZ-8........................................................................44 2.2.2.2.3. Agarose gel electrophoresis...............................................44 2.2.2.2.4. Determination of rLZ-8 binding content on gold nanoparticles..................................................................45 2.2.2.2.5. SDS-PAGE confirm and western blotting........................45 2.2.2.2.6. Desorption kinetics characterization of rLZ-8 from the GNP surface...................................................................46 2.2.3. Macrophage activation induced by GNP-LZ-8.......................................46 2.2.3.1. Primary cell cultures...................................................................46 2.2.3.2. Determination of IL-1β and IL-10 production............................47 2.2.3.3. Fluorescence-activated cell sorting (FACS) analysis..................48 2.2.3.4. Statistical analysis.......................................................................49 2.2.4. Splenocyte activation induced by GNP-LZ-8..........................................49 2.2.4.1. Preparation of primary splenocytes.............................................49 2.2.4.2. Purification of T cells..................................................................50 2.2.4.3. BrdU incorporation assay............................................................51 2.2.4.4. Determination of IFN-γ, IL-2 cytokine production.....................51 2.2.4.5. Fluorescence-activated cell sorting (FACS)................................52 2.2.4.6. Quantitative real-time PCR.........................................................53 2.2.4.7. Statistical analysis.......................................................................54 Chapter 3. Results..........................................................................................................56 3.1. Functional expression and detection of rLZ-8...................................................56 3.2. Synthesis of gold nanoparticles with different sizes...........................................56 3.3. rLZ-8 protein adsorption on gold nanoparticles................................................57 3.3.1. Optimal conjugation pH adjustment of GNPs solution........................57 3.3.2. Optimal protein concentration for GNP-LZ-8 conjugation..................58 3.4. Characteristics of GNP-LZ-8 with different sizes.............................................59 3.4.1. Size distribution of GNP-LZ-8..............................................................59 3.4.2. Stability of GNP-LZ-8 dispersions3.4.3. LZ-8 loading analysis of GNP-LZ-8...........................................................................................59 3.4.3. LZ-8 loading analysis of GNP-LZ-8.....................................................60 3.4.4. Desorption kinetics characterization of LZ-8 on GNP-LZ-8 surface...61 3.5. Mouse peritoneal macrophage activation induced by GNP-LZ-8.....................61 3.5.1. GNP-LZ-8 treatment induced IL-1β production on macrophages........62 3.5.2. GNP-LZ-8 uptake induced side scatter change on macrophages..........62 3.5.3. GNP-LZ-8 treatment induced CD 80/86 and MHC class II expression on macrophages in a size dependent manner....................................63 3.6. Mouse splenocyte activation induced by GNP-LZ-8........................................64 3.6.1. GNP-LZ-8 treatment induced splenocyte proliferation........................64 3.6.2. GNP-LZ-8 treatment induced IL-2 and INF-γ production on Splenocyte.............................................................................................65 3.6.3. GNP-LZ-8 uptake induced side scatter change on splenocyte.............65 3.6.4.GNP-LZ-8 treatment induced CD25 expression on CD3+ and CD3- splenocyte.............................................................................................66 Chapter 4. Discussions...................................................................................................68 4.1. Functional Expression of rLZ-8 in S. cerevisiae................................................68 4.2. Synthesis of gold nanoparticles with different sizes..........................................69 4.3. Labeling of rLZ-8 on the surface of GNPs........................................................70 4.3.1. Optimal pH and rLZ-8 concentration for GNP-LZ-8 synthesis............70 4.3.2. Quantity and stability of rLZ-8 on GNP surface...................................72 4.4. Multivalence of LZ-8 on GNPs enhance the immunomodulatory effect on macrophages in a size dependent manner........................................................73 4.5. Multivalence of LZ-8 on GNPs enhance the immunomodulatory effect on splenocytes in otherwise special size dependent manner................................75 4.6. Possible physiological roles of GNP-LZ-8 sizes...............................................77 4.7. Conclusions........................................................................................................78 5. References...................................................................................................................79 Table and Figure Content Figure 1. Functional expression and characteristics of rLZ-8.........................................98 Figure 2. UV-Vis absorbance measurements of GNPs with shift of the surface plasmon band peaks correlating with GNPs of different sizes.......................................................99 Figure 3. Absorbance observation on the various sizes of GNPs (1:0.3-1:2.5) coated with rLZ-8 in different pH condition.............................................................................100 Figure 4. Absorbance derivative before and after addition of 2%NaCl on the various sizes of GNPs (1:0.3-1:2.5) coated with different rLZ-8 concentration........................101 Figure 5. (A) Agarose gel electrophoresis of GNP-LZ-8 with different sizes (B) Zeta potential measurements of GNP-LZ-8..........................................................................102 Figure 6. rLZ-8 loading analysis of GNP-LZ-8............................................................103 Figure 7. (A) SDS-PAGE analysis of GNP-LZ-8 with different sizes (B) Western blotting by using mouse anti-LZ-8 monoclonal antibodies...........................................104 Figure 8. Desorption kinetics characterization of rLZ-8 from the GNP surface in (A) phosphate buffer solution (pH 7.4) and (B) complete DMEM cellculture media.........105 Figure 9. IL-1β production enhancement of GNP-LZ-8 with various sizes..................106 Figure 10. Side scatter variation of macropahges treated with various sizes of GNP-LZ-8 in a size-dependent manner......................................................................107 Figure 11. CD 80 variation of macropahges treated with various sizes of GNP-LZ-8.................................................................................................................... 108 Figure 12. CD 86 variation of macropahges treated with various sizes of GNP-LZ-8......................................................................................................................109 Figure 13. MHC class II variation of macropahges treated with various sizes of GNP-LZ-8......................................................................................................................110 Figure 14. BrdU incorporation enhancement of GNP-LZ-8 with various sizes............111 Figure 15. IL-2 production enhancement of GNP-LZ-8 with various sizes..................112 Figure 16. IL-2 production enhancement of GNP-LZ-8 with various sizes..................113 Figure 17. Side scatter variation of splenocytes treated with various sizes of GNP-LZ-8 in a size-dependent manner............................................................................................114 Figure 18. CD25 upregulation on CD3+ and CD3- splenocytes treated with various sizes of GNP-LZ-8.................................................................................................................115 Table 1. Endotoxin contents of LZ-8 and PS-G measured by LAL gel-clot assay.....116 Table 2. Characterization of GNP-LZ-8 with different sizes.........................................117 Table 3. MFI of CD3+CD25+ and CD3-CD25+ splenocytes.......................................118 | |
dc.language.iso | en | |
dc.title | 靈芝蛋白Ling Zhi-8鍵結於奈米金粒子促進巨噬細胞及脾細胞免疫調節能力與其粒徑依賴性質之探討 | zh_TW |
dc.title | Multivalency of Ling Zhi-8 coated Gold Nanoparticles Enhances the Immunomodulatory Response on Macrophages and Splenocytes As a Function of Size | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 許先業,周志輝,劉?叡,繆希椿 | |
dc.subject.keyword | 奈米金粒子,靈芝,LZ-8,免疫調節蛋白,巨噬細胞活化,脾細胞活化, | zh_TW |
dc.subject.keyword | gold nanoparticles,Immunomodulatory protein,Ganoderma lucidum,Ling Zhi-8,macrophage activation,splenocyte activation, | en |
dc.relation.page | 118 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2010-08-04 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝學研究所 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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