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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳益群(Yi-Chun Wu) | |
dc.contributor.author | Wen-Chin Huang | en |
dc.contributor.author | 黃文錦 | zh_TW |
dc.date.accessioned | 2021-06-08T04:15:48Z | - |
dc.date.copyright | 2010-08-11 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-04 | |
dc.identifier.citation | References
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22338 | - |
dc.description.abstract | Cell migration plays an important role during animal development. The gonadogenesis of the C. elegans hermaphrodite is guided by stage-specific migration of two leader cells, distal tip cells (DTCs). These cells undergo three sequential phases of migration-centrifugal, dorsal and centripetal-during larva stages and lead the formation of U-shaped gonad arm. Although the guidance cue netrin has been shown to control the dorsal migration of the DTCs, the guidance systems that regulate their longitudinal migration is still unclear. In addition, the temporal control of these spatial migrations is poorly understood. We find that MOM-5, a Frizzled receptor (Fz), is required for the proper DTC centripetal migration specifically at the 4th larval stage and that all five wnts are involved in this process. Four of the wnts signal to MOM-5 in promoting correct pathfinding, while the other one seems to act in suppression of MOM-5. Using the gfp reporter, we find that mom-5 is transcriptionally upregulated in the DTCs at the 4th larval stage, consistent with its cell-autonomous function in DTC centripetal migration. Overexpression of MOM-5/Fz using various promotors results in the L4-specific pathfinding defect, indicating that the regulation of the MOM-5/Fz level is critical for the correct centripetal migration at L4. Moreover, our genetic analysis suggests that MOM-5/Fz may acts upstream of DSH-1/Dsh and CED-5/GEF, but in paralle to CED-2/CrkII, in the signaling pathway to regulate the L4-specific DTC centripetal migration, at least in the anterior DTC migration Together, our work shows that MOM-5/Fz acts with specific wnts to guide the centripetal migration of DTCs at the 4th larval stage and may define a novel signaling pathway involving CED-5/GEF but not CED-2/CrkII | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T04:15:48Z (GMT). No. of bitstreams: 1 ntu-99-R97b43026-1.pdf: 3111192 bytes, checksum: 9db976b1adb2e7a607abf52f343a6bcb (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | Table of Contents
致謝 i 中文摘要 ii 英文摘要 iii Table of Contents 1 Introduction 4 Materials and Methods 9 Strains & Genetics 9 Transgenic lines 9 Molecular biology 10 RNA interference (RNAi) 13 Analysis of phenotypes 14 Results 15 The Frizzled receptor MOM-5 is required for correct DTC pathfinding 15 Different Wnts act redundantly to guide anterior and posterior DTCs and may function as repellents and attractants 17 Wnts are instructive guidance cues 20 mom-5 acts with distinct wnts in anterior and posterior DTCs 21 mom-5 is transcriptionally upregulated in DTC phase III migration at the L4 stage 23 Overexpression of MOM-5/Fz also cause phase III pathfinding defect, and is not sufficient to affect centripetal DTC migration 24 dsh-1 and other canonical wnt pathway components also show defects in DTC path finding 26 mom-5/Fz may act upstream of dsh-1/Dsh, ced-5/GEF and ced-10/Rac to regulate phase III path finding of DTCs. 27 Discussion 31 MOM-5 may be repressed in phase I and upregulated at L4 stage 31 The opposing responses make symmetrical gonad morphogenesis 33 The opposite trend of L4 phase III pathfinding defect of anterior and posterior DTCs in mom-5 mutant 34 References 37 Figures 44 Figure 1. The pathfinding defect of mom-5 and egl-20 44 Figue 2. mom-5 is required for proper phase III path finding 45 Figure 3. Wnts act redundantly to guide the phase III path finding of DTCs 46 Figure 4. mom-5 acts with distinct wnts in anterior and posterior DTCs 48 Figure 5. mom-5 is transcriptionally upregulated in DTC phase III migration at the L4 stage 49 Figure 6. MOM-5 translational fusion GFP 50 Figure 7. Overexpression of MOM-5/Fz is not sufficient to affect centripetal DTC migration 51 Figure 8. mom-5 and dsh-1 act in the same pathway with ced-5 and ced-10 52 Figure 9. Model of DTC phase III migration 53 Tables 54 Table 1. The pathfinding defect occurs at 4th larva stage in both mom-5 and ced-5 mutants 54 Table 2. MOM-5 is up-regulated at 4th larva stage 55 Table 3. Ectopic expression of Wnts and MOM-5 56 Table 4. Mutation in conanical Wnt pathway causes phase III pathfinding defect 57 Table 5. ced-5 and ced-10 rescue dsh-1 during DTCs phase III migration 58 | |
dc.language.iso | en | |
dc.title | 線蟲性腺發育過程中MOM-5/Frizzled 在前後端遠頂細胞啟動相反的訊息去引導其遷移 | zh_TW |
dc.title | MOM-5/Frizzled-mediated opposing signals direct distal tip cell migration during gonadogenesis in Caenorhabditis elegans | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳昌熙,汪宏達 | |
dc.subject.keyword | 線蟲,細胞遷移, | zh_TW |
dc.subject.keyword | mom-5,C. elegans,distal tip cell (DTC),cell migration,Wnt signaling, | en |
dc.relation.page | 58 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2010-08-05 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
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