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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃榮南(Rong-Nan Huang) | |
| dc.contributor.author | Xoong Harng Oon | en |
| dc.contributor.author | 溫松翰 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:46:02Z | - |
| dc.date.copyright | 2019-02-15 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-02-12 | |
| dc.identifier.citation | Arumugam G, Swamy MK, Sinniah UR. 2016. Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules 21:369. doi: 10.3390/molecules21040369.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21770 | - |
| dc.description.abstract | 精油 (Essential oils, EOs) 為許多植物產生的次級代謝物，可能和對抗其天敵的防衛機制相關。精油的藥用價值已廣為人知，如芳香、治療用途、抗菌性能、昆蟲忌避和阻礙效果等，因此這些揮發性化合物在農業害蟲的管理應有很大的應用價值。精油是環境友善及在自然界中是可生物分解的，然而，因其可在短時間內經由蒸發、氧化和聚合作用等因素而快速降解而不受重視，所以迫切需要評估這些現存的問題。微膠囊化技術因其具有緩慢釋放效果常用於製藥工業，但在有害生物管理較少使用。本研究評估微膠囊化的左手香 (Plectranthus amboinicus (Lour.) Spreng.) 精油對於桃蚜 (Myzus persicae (Sulzer)) 與小菜蛾 (Plutella xylostella (Linnaeus)) 的防治效果。左手香精油主要的有效成分由14種合化合物組成，將其中三種含量最多的成分carvacrol, ρ-cymene 及β-caryophyllene以海藻酸鈉 (sodium alginate (SA)) 包埋，經氣相層析質譜儀 (Gas Chromatography-Mass Spectrophotometer, GC-MS) 及固相微萃取 (Solid Phase Microextraction, SPME) 分析0.5% SA 包埋的精油，ρ-cymene 在0~72小時內以高濃度釋放，β-caryophyllene 則相反；Carvacrol在各時段的釋放情形相同。膠囊化的效率測試結果顯示SA的濃度越高 (2%) 則效率越佳，但膠囊越大。再者，生物檢定結果顯示膠囊化的精油以劑量應變方式連續五天對桃蚜與小菜蛾具燻蒸毒性。然而，在開放空間下，膠囊化的精油對桃蚜的效果會因蒸氣會向多方向散逸而下降。反之，對小菜蛾的產卵阻礙檢定有不錯的效果，但對桃蚜的忌避檢定則不顯著。 | zh_TW |
| dc.description.abstract | Essential oils (EOs) are secondary metabolites produced by many plants which are found possibly crucial in defense mechanism against its natural enemies. They are also widely known for their medicinal, fragrant, therapeutic use, antimicrobial properties, insect repellent and deterrent effects. Thus, these volatile have great values towards the management of agricultural pest. Essential oils are environmental friendly and biodegradable in nature, however, essential oil has limited application due to rapid degradation over a short duration via vaporization, oxidation, polymerization, etc. This problem needs to be addressed. Microencapsulation technologies are commonly used in pharmaceutical industry because of its slow release effects, but less common in pest management. In this study, we evaluated the effect of encapsulated Plectranthus amboinicus (Lour.) Spreng essential oil towards Myzus persicae (Sulzer) and Plutella xylostella (Linnaeus). The active ingredients of Pl. amboinicus essential oils comprise about 14 complex compounds and three major components (carvacrol, ρ-cymene and β-caryophyllene) were encapsulated within sodium alginate (SA). Gas Chromatography-Mass Spectrophotometer (GCMS) analysis with Solid Phase Microextraction (SPME) adsorption founded that in 0.5% SA encapsulated oils, ρ-cymene was released in higher concentration at 0-72 hours whereas β-caryophyllene was the lowest. Carvacrol remains constant at all times. Encapsulation efficiency test displayed that the higher the concentration of SA (2%) the better is the encapsulating efficiency and the bigger is the size of the capsules. Bioassay results showed that encapsulated oils exhibits fumigant toxicity to M. persicae and P. xylostella for five consecutive days in a dose dependent manner. However, the effectiveness of encapsulated EOs towards M. persicae declined accordingly in open space that allows the fumes to escape at multiple directions. Oviposition deterrent assays with P. xylostella showed promising effects, while repellent assays were not significant towards M. persicae. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:46:02Z (GMT). No. of bitstreams: 1 ntu-108-R05632015-1.pdf: 2549005 bytes, checksum: 5f4910f0782943110ff2d5e71d3bfee7 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 口試委員審定書 i
Acknowledgement ii 中文摘要 iii Abstract iv Table of Contents v List of Tables vii List of Figures viii Table of Appendixes x Chapter 1: Introduction 1 1.1 Essential oils 1 1.2 Plectranthus amboinicus and it’s essential oil 2 1.3 Green Peach Aphid M. persicae 4 1.4 Diamondback Moth (DBM) P. xylostella 5 1.5 Gas Chromatography Mass Spectrometry (GC-MS) and Solid Phase Microextraction (SPME) 6 1.6 Encapsulation of essential oils 7 1.7 Y- Olfactometer 8 1.8 Research aim 8 Chapter 2: Materials and Methods 10 2.1 Insect rearing of M. persicae and P. xylostella 10 2.2 Plectranthus amboinicus essential oil extraction and analysis 10 2.3 Encapsulation of Pl. amboinicus essential oil 11 2.4 The release kinetics of encapsulated essential oil 12 2.5 Repellent bioassay against M. persicae 13 2.6 Fumigant toxicity bioassay against M. persicae 14 2.7 Ovipositional deterrent bioassay against P. xylostella 17 2.8 Fumigant toxicity bioassay against P. xylostella 18 2.9 Statistical analysis 18 Chapter 3: Results 19 3.1 Characterization of SA capsules with essential oil 19 3.2 Repellent bioassay using M. persicae 19 3.3 Fumigant toxicity bioassay against M. persicae 20 3.4 Ovipositional deterrent bioassay against DBM P. xylostella 22 3.5 Fumigant toxicity bioassay against P. xylostella 22 Chapter 4: Discussion 24 4.1 Characterization of essential oil encapsulated SA capsule 24 4.2 Experimental design for repellent and fumigant toxicity bioassay against M. persicae 25 4.3 Experimental design for ovipositional deterrent and fumigant toxicity bioassay against P. xylostella 26 4.4 Relationship of GC-MS results and bioassay results 27 4.5 Future work 28 References 29 Appendixes 55 | |
| dc.language.iso | en | |
| dc.title | 海藻膠包埋左手香精油主要成分對桃蚜與小菜蛾的影響 | zh_TW |
| dc.title | Effects of Encapsulated Main Components of Plectranthus amboinicus Essential Oils Towards Myzus persicae and Plutella xylostella | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳文哲(Wen-Jer Wu),許如君(Ju-Chun Hsu),蔡坤憲(Kun-Hsien Tsai),黃旌集(Chin-Gi Huang) | |
| dc.subject.keyword | 精油,微膠囊化,左手香,桃蚜, | zh_TW |
| dc.subject.keyword | essential oil,microencapsulation,Plectranthus amboinicus,Myzus persicae, | en |
| dc.relation.page | 80 | |
| dc.identifier.doi | 10.6342/NTU201900370 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2019-02-12 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
| 顯示於系所單位： | 昆蟲學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-108-1.pdf 目前未授權公開取用 | 2.49 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。