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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳蕙芬 | |
| dc.contributor.author | Chun-Yang Chang | en |
| dc.contributor.author | 張鈞暘 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:45:57Z | - |
| dc.date.copyright | 2019-02-15 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-02-12 | |
| dc.identifier.citation | 1. Durand, S. and G. Storz, Reprogramming of anaerobic metabolism by the FnrS small RNA. Mol Microbiol, 2010. 75(5): p. 1215-31.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21768 | - |
| dc.description.abstract | 大腸桿菌中的 glutaredoxin-4 (Grx4) 編碼基因為 grxD，為目前唯一在其中發現的單巰 glutaredoxin (僅具 CGFS 的活性區域)。本篇研究利用 grxD-lacZ 融合基因及染色體中 Grx4-3xFLAG 觀察 grxD 的調控，當 small RNA大量表現時，FnrS 負向調控 grxD-lacZ 達原本約 1/2 的表現量，在 Grx4-3xFLAG 蛋白質層面，則看到更明顯的抑制。RyhB 亦負向調控 grxD-lacZ 約 20% 的表現。除此之外，轉錄因子缺失狀態下，發現 NsrR 參與 grxD 的正向調控，nsrR 缺失造成表現量下降近 1/2 ，而 Grx4-3xFLAG 蛋白質層面則影響更鉅。因此 grxD-lacZ 的表現受到如：氧、鐵、一氧化氮相關因子等的調控，也在 Grx4-3xFLAG 對生長產生的影響指出這部分的精細調控的重要性。
除了基因調控的研究之外，在 E. coli 基因工程中 P1 噬菌體扮演重要的地位，必須要用於建構上述各種突變株，故本篇論文亦克服之前 P1 容易失活的問題，利用新的增殖方式使 P1 在基因工程操作上運作順利。最後，藉由之前對 SulA、ClpYQ 的深度研究，我們開發了一套「新穎之轉形及轉殖效率增加與抑制細胞分裂技術」，也期望能夠應用在基因工程、微生物族群分析等研究上。 | zh_TW |
| dc.description.abstract | The glutaredoxin-4 (Grx4)-encoding in grxD gene in Escherichia coli, which is the only monothiol glutaredoxin (only active region with CGFS) found in it. In this study, grxD-lacZ fusion gene and Grx4-3xFLAG in chromosome were used to observe the regulation of grxD. When small RNA was expressed in a high level, FnrS negatively regulated grxD-lacZ to about 1/2 of the original expression, at the level of Grx4-3xFLAG protein the more obvious repression happend. RyhB also negatively regulates the performance of grxD-lacZ by ~20%. In addition, in the absence of transcription factors, NsrR was found to be involved in the positive regulation of grxD, and nsrR deletion caused a decrease in performance by nearly 1/2, while the Grx4-3xFLAG protein level was more influential. Therefore, the performance of grxD-lacZ is regulated by factors such as oxygen, iron, and nitric oxide related factors, and the influence of Grx4-3xFLAG on growth indicates the importance of fine regulation of this part.
In addition to gene regulation studies, P1 phage plays an important role in E. coli genetic engineering and be used to construct these various mutants. Therefore, this paper also overcomes the problem of easy inactivation of P1 before, and utilizes new proliferation, the way to make P1 work well in genetic engineering operations. Finally, with the previous in-depth study of SulA and ClpYQ, we have developed a set of 'new enhance cloning and transformation efficiency method and cell division inhibition technology' and expected to be applied in genetic engineering, microbial population analysis and other research. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:45:57Z (GMT). No. of bitstreams: 1 ntu-108-D97623005-1.pdf: 3772817 bytes, checksum: 020022fe795727bfd0bd41cd9bcf0959 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 中文摘要 vi
Abstract vii 已發表文章 viii 一、 前言 1 1.1 活性氧類 (Reactive Oxygen Species) 1 1.2 氧化壓力 (Oxidative Stress) 及硝化壓力 (Nitrosative Stress) 3 1.3 氧化還原調控 (Redox regulation) 3 1.4 Glutaredoxin系統 4 1.5 Grx4 簡介 6 1.6 鐵硫簇 (Iron-sulfur cluster, ISC) 8 1.7 E. coli 中鐵之調控 9 1.7.1 Fur： 9 1.7.2 RyhB： 10 1.8 E. coli 呼吸路徑之調控因子 – FNR 10 1.9 E. coli 呼吸路徑之調控因子 – FnrS 11 1.10 E. coli 一氧化氮相關之調控因子 12 1.11 E. coli Grx4可能的交互作用蛋白質 – BolA 13 1.12 生物膜形成及造成的影響 13 二、 材料與方法 15 2.1 一般研究材料 15 2.1.1 菌株及質體 15 2.1.2 藥品及試劑 16 2.1.3 器材設備 17 2.1.4 分析軟體 17 2.2 一般實驗方法 18 2.2.1 質體製備及轉殖 18 2.2.2 質體製備 18 2.2.3 勝任細胞 ( competent cell ) 製備 19 2.2.4 轉形作用 ( transformation ) 20 2.3 選殖基因表現系統之建立 23 2.3.1 載體 (vector) 的製備 23 2.3.2 選殖基因片段之製備 25 2.3.3 選殖片段之處理 26 2.3.4 接合反應 (ligation reaction) 26 2.3.5 grxD promoter 點突變之質體建構 27 2.4 噬菌體一般實驗方法 27 2.4.1 製備嗜菌體 plate lysates (Preparation of phage plate lysate) 28 2.4.2 Titer the lysate 28 2.4.3 P1 轉導作用 ( P1 transduction ) 29 2.4.4 λ的特定轉導作用 (specialize transduction) 31 2.5 建構基因剃除 (knock out) 與 3x-FLAG 突變株 33 2.6 β-galactosidase 活性分析 36 2.7 西方墨點分析 (Western Blotting) 38 三、 改進噬菌體 P1 增殖方式 40 3.1 前言 40 3.2 材料與方法 42 3.2.1 P1 phage 增殖與濃縮 42 3.3 結果 44 3.3.1 增殖與濃縮方法 P1 phage增殖的效果 44 3.3.2 增殖與濃縮方法中添加Yeast extract對 P1 phage增殖的效果 44 3.4 討論 45 3.4.1 增殖與濃縮方式及添加Yeast extract對 P1 phage的影響 45 四、 大腸桿菌 grxD 基因表現之調控 46 4.1 研究動機與目的 46 4.2 結果 46 4.2.1 歸納與繪製 grxD 上游的 promoter 區域及 cis-element 位置 46 4.2.2 氧含量相關因子抑制 grxD 表現 (FnrS and/or FNR) 47 4.2.3 鐵含量相關因子調控 grxD 表現 (RyhB and/or Fur) 49 4.2.4 一氧化氮相關因子促進 grxD 表現 (NsrR) 50 Grx4-3xFLAG 對生長的影響 52 4.2.5 探討 Grx4 與 BolA 交互作用的意義 53 4.2.6 BolA 與 Grx4 的交互作用現象 53 4.3 討論 54 4.3.1 grxD 受到small RNA 所調控 54 4.3.2 grxD 受到多重因子所調控 54 4.3.3 grxD 具有兩個以上的promoter 55 4.3.4 Grx4 無法建構缺失突變株 55 五、 新穎之轉形及轉殖效率增加與抑制細胞分裂技術 57 5.1 發明所欲解決之問題 57 5.1.1 先前技術之轉形效率可再提升： 57 5.1.2 先前技術之選殖偽陽性率過高： 57 5.1.3 TA選殖(TA cloning)效率及許多使用問題仍待改善： 58 5.1.4 先前技術因受限於轉形效率及偽陽性過高造成選殖的整體問題： 59 5.2 解決問題之技術手段 60 5.2.1 染色體同源重組菌株提高轉形效率 60 5.2.2 藉由抑制細胞分裂減少選殖偽陽性率 60 5.2.3 本專利可進行無縫選殖(Seamless cloning) 60 六、 參考文獻 64 | |
| dc.language.iso | zh-TW | |
| dc.title | 大腸桿菌中 Grx4 蛋白質特性及其與 ClpYQ 的交互關係 | zh_TW |
| dc.title | The characteristics of Grx4 and its interaction with ClpYQ protease in Escherichia coli | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 邱志郁,黃思蓴,陳建德,王鳳英 | |
| dc.subject.keyword | grxD,Grx4,glutaredoxin,FnrS,RyhB,NsrR,氧,鐵,一氧化氮,SulA,ClpYQ, | zh_TW |
| dc.subject.keyword | grxD,Grx4,glutaredoxin,FnrS,RyhB,NsrR,Oxygen,iron,nitric oxide,SulA,ClpYQ, | en |
| dc.relation.page | 99 | |
| dc.identifier.doi | 10.6342/NTU201900467 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2019-02-12 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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