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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃鵬鵬(Pung-Pung Hwang) | |
| dc.contributor.author | Shang-Wu Shih | en |
| dc.contributor.author | 施尚武 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:37:42Z | - |
| dc.date.copyright | 2019-07-31 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-07-22 | |
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Regul. Integr. Comp. Physiol. 302, R283-291. Lu, S. W. (2017). The role of estrogen-related receptors in regulation of proton secretion in medaka (Oryzias latipes). National Taiwan University, Taipei, Taiwan. Murray, J., Auwerx, J. & Huss, J. M. (2013). Impaired myogenesis in estrogen-related receptor γ (ERRγ)-deficient skeletal myocytes due to oxidative stress. FASEB. J. 27, 135-150. Pei, L., Mu, Y., Leblanc, M., Alaynick, W., Barish, G. D., Pankratz, M., Tseng, T. W., Kaufman, S., Liddle, C., Yu, R. T., Downes, M., Pfaff, S. L., Auwerx, J., Gage, F. H. & Evans, R. M. (2015). Dependence of hippocampal function on ERRγ-regulated mitochondrial metabolism. Cell Metab. 21, 628-636. Poidatz, D., Dos Santos, E., Gronier, H., Vialard, F., Maury, B., De Mazancourt, P. & Dieudonné, M. N. (2015). Trophoblast syncytialisation necessitates mitochondrial function through estrogen-related receptor-γ activation. Mol. Hum. Reprod. 21, 206-216. Rossi, M., Colecchia, D., Iavarone, C., Strambi, A., Piccioni, F., Verrotti di Pianella, A. & Chiariello, M. (2011). Extracellular signal-regulated kinase 8 (ERK8) controls estrogen-related receptor α (ERRα) cellular localization and inhibits its transcriptional activity. J. Biol. Chem. 286, 8507-8522. Shen, W. P., Horng, J. L. & Lin, L. Y. (2011). Functional plasticity of mitochondrion-rich cells in the skin of euryhaline medaka larvae (Oryzias latipes) subjected to salinity changes. Am. J. Physiol. Regul. Integr. Comp. Physiol. 300, R858-868. Sonoda, J., Laganière, J., Mehl, I. R., Barish, G. D., Chong, L. W., Li, X., Scheffler, I. E., Mock, D. C., Bataille, A. R., Robert, F., Lee, C. H., Giguère, V. & Evans, R. M. (2007). Nuclear receptor ERRα and coactivator PGC-1β are effectors of IFN-γ induced host defense. Genes Dev. 21, 1909-1920. Tremblay, A. M., Dufour, C. R., Ghahremani, M., Reudelhuber, T. L. & Giguère, V. (2010). Physiological genomics identifies estrogen-related receptor alpha as a regulator of renal sodium and potassium homeostasis and the renin-angiotensin pathway. Mol. Endocrinol. 24, 22-32. Tremblay, A. M. & Giguère, V. (2007). The NR3B subgroup: an ovERRview. Nucl. Recept. Signal. 5, e009. Tremblay, G. B., Kunath, T., Bergeron, D., Lapointe, L., Champigny, C., Bader, J. A., Rossant, J. & Giguère, V. (2001). Diethylstilbestrol regulates trophoblast stem cell differentiation as a ligand of orphan nuclear receptor ERR beta. Genes Dev. 15, 833-838. Wang, D., Wang, Y., Liu, F. Q., Yuan, Z. Y. & Mu, J. J. (2016). High salt diet affects renal sodium excretion and ERRα expression. Int. J. Mol. Sci. 17, 480-491. Yang, N., Shigeta, H., Shi, H. & Teng, C. T. (1996). Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271, 5795-5804. Yu, D. D. & Forman, B. M. (2005). Identification of an agonist ligand for estrogen-related receptors ERRbeta/gamma. Bioorg. Med. Chem. Lett. 15, 1311-1313. Zhao, J., Lupino, K., Wilkins, B. J., Qiu, C., Liu, J., Omura, Y., Allred, A. L., McDonald, C., Susztak, K., Barish, G. D. & Pei, L. (2018). Genomic integration of ERRγ-HNF1β regulates renal bioenergetics and prevents chronic kidney disease. Proc. Natl. Acad. Sci. U. S. A. 115, E4910-4919. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21553 | - |
| dc.description.abstract | 廣鹽性硬骨魚類能夠生存在廣泛的鹽度範圍內，包含淡水及海水。成魚的鰓或胚胎表皮上的離子細胞是富含粒線體的特化細胞，能夠表現不同的離子運輸蛋白來運輸離子。這些細胞在淡水中負責吸收離子，在海水中則負責排出離子，藉此來維持體內體液的離子組成。先前研究已經提出了在廣鹽性青鱂魚中淡水型離子細胞和海水型離子細胞的模型，但仍無法完整解釋其離子調節的詳細機制。因此，發掘可能參與離子調控的因子是一項非常重要且有助於全面了解魚類如何適應變動環境的議題。類雌激素受體(ERR)是一種孤兒核受體，能夠作為轉錄因子來調控其他基因的表現，包括與能量代謝有關的基因。過去研究顯示，哺乳類的ERRα和ERRγ可以調控離子運輸蛋白的表現，暗示ERR家族可能參與離子的調控。因此，本研究使用印度種青鱂魚作為實驗物種來探討ERR家族在魚類離子調控中所扮演的角色。在將青鱂魚成魚及胚胎從淡水轉移到海水過後，ERRα、ERRβ1、ERRγ1和 ERRγ2在胚胎及成魚鰓的基因表現量皆顯著下降，表示ERR家族對青鱂魚在淡水的適應可能非常重要。接著，在原位雜交及免疫組織化學染色的結果指出，不論在成魚的鰓或是胚胎的表皮上，ERRγ2的mRNA表現大部分都和鈉鉀離子幫浦的蛋白質表現位置相同，表示ERRγ2會表現在大部分的離子細胞中。此外，弱化胚胎ERRγ2的表現會造成體內鈉、氯離子的含量降低，同時也降低離子細胞對鈉離子吸收的速率，但卻不影響胚胎表皮離子細胞的數目、耗氧及排銨速率。總結而言，本研究認為表現在離子細胞上的ERRγ2可能會參與鹽類的吸收機制，以利青鱂魚適應淡水。然而，ERRγ2如何調控離子細胞吸收鹽類的詳細機制尚不清楚，仍需在未來研究中更進一步釐清。 | zh_TW |
| dc.description.abstract | Euryhaline teleosts are capable of surviving within a wide range of salinities including in freshwater (FW) and seawater (SW). Ionocytes in fish gills or embryonic skin are specialized mitochondrion-rich cells which express different ion transporters to transport ions. These cells are responsible for ion absorption and secretion to maintain ion compositions of body fluids in FW and SW, respectively. The basic regulatory model of FW and SW types of ionocytes in medaka has been proposed, but the detailed ionoregulatory mechanisms are still not explained well. Thus, discovering possible factors involved in ion regulation is an important issue and is helpful to completely understand how fishes acclimate to fluctuating environments. Estrogen-related receptors (ERRs), steroid hormone receptors, are orphan nuclear receptors, functioning as transcription factors to regulate the expression of other genes, including energy metabolism-related genes. Previous studies showed that ERRα and ERRγ regulate the expression of ion transporters in mammals, implying that ERRs might involve in ion regulation. Therefore, this study used Indian medaka (Oryzias melastigma), euryhaline teleosts, as experimental organisms to investigate the role of ERRs in ion regulation of fishes. The mRNA expression of errα, errβ1, errγ1, and errγ2 in medaka embryos and adult gills were down-regulated after transfer from FW to SW, suggesting that ERRs may play roles in acclimation to FW. Besides, the results of in situ hybridization showed the mRNA signals of errγ2 were mostly co-localized with the immunohistochemistry signals of ionocyte marker, Na+-K+ ATPase (NKA), in both embryos and adult gills in FW, indicating that most part of ERRγ2 is expressed in the ionocyte. Moreover, ERRγ2 knockdown in embryos reduced the Na+/Cl- contents and Na+ influxes of ionocytes, but did not affect the ionocyte numbers in the embryonic skin, O2 consumption rate, and NH4+ secretion rate in the embryos. Overall, the present study suggested that ERRγ2 expressed in ionocytes may participate in salt-absorption mechanisms to adapt to FW. However, how ERRγ2 regulates the salt-absorption of ionocytes is still unclear and will be further investigated in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:37:42Z (GMT). No. of bitstreams: 1 ntu-108-R06B45004-1.pdf: 1782283 bytes, checksum: ba4057fd3d9f0ac565ca81de6f3cfa6e (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 謝辭................................................................................................i
中文摘要 ...........................................................................................ii Abstract..........................................................................................iii Contents............................................................................................v List of tables...................................................................................viii List of figures....................................................................................ix 1. Introduction................................................................................1 1.1 Fish ion regulation.........................................................................1 1.2 Estrogen-related receptor (ERR).............................................................2 1.2.1 Structural feature and transcriptional activity of ERRs.....................................3 1.2.2 ERRs as regulators of energy metabolism.....................................................4 1.2.3 ERRs as regulators of ionic homeostasis/acid-base balance...................................5 1.3 Indian medaka as a model to study fish ion regulation.......................................6 1.4 Purpose.....................................................................................7 2. Materials and methods.......................................................................8 2.1 Experimental animals........................................................................8 2.2 Acclimation experiments.....................................................................8 2.3 Isolation of total RNA......................................................................8 2.4 Reverse-transcription polymerase chain reaction (RT-PCR) analysis...........................9 2.5 Quantitative PCR (qPCR).....................................................................9 2.6 Sample fixation and cryosectioning.........................................................10 2.7 In situ hybridization (ISH)................................................................10 2.8 Immunohistochemistry (IHC).................................................................11 2.9 Morpholino oligonucleotide (MO) knockdown..................................................12 2.10 Measurements of whole-body ion contents....................................................12 2.11 Scanning ion-selective electrode technique (SIET)..........................................13 2.12 Measurements of O2 consumption rate and NH4+ secretion rate................................14 2.13 Agonist experiments........................................................................14 2.14 Statistical analysis.......................................................................15 3. Results....................................................................................16 3.1 Effects of salinity change on the mRNA expression of ERRs in medaka........................16 3.2 Tissue distribution of ERRs in FW-acclimated medaka........................................16 3.3 Localization of ERRγ2 in FW-acclimated medaka..............................................16 3.4 Effects of ERRγ2 knockdown on the whole-body ion contents..................................17 3.5 Effects of ERRγ2 knockdown on the ion influxes of ionocytes................................17 3.6 Effects of ERRγ2 knockdown on the ionocyte numbers.........................................18 3.7 Effects of ERRγ2 knockdown on O2 consumption rate and NH4+ excretion rate..................18 3.8 Effects of ERRγ2 agonist on the whole-body ion contents and the ion influxes of ionocytes..18 4. Discussion.................................................................................20 4.1 Potential of ERRs for ion regulation.......................................................20 4.2 Expression of ERRγ2 in ionocytes...........................................................20 4.3 Actions of ERRγ2 on internal Na+ and Cl- homeostasis.......................................21 4.4 Relation between ion regulation and energy metabolism in ionocytes.........................23 5. Conclusion and perspectives................................................................25 References.........................................................................................26 Tables and figures.................................................................................32 | |
| dc.language.iso | en | |
| dc.title | 類雌激素受體在青鱂魚離子調控之角色 | zh_TW |
| dc.title | The role of estrogen-related receptors in ion regulation of medaka (Oryzias melastigma) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 曾庸哲(Yung-Che Tseng),周銘翊(Ming-Yi Chou),韓玉山(Yu-San Han),邵奕達(Yi-Ta Shao) | |
| dc.subject.keyword | 類雌激素受體,離子調控,鹽類吸收,離子細胞,青?魚, | zh_TW |
| dc.subject.keyword | estrogen-related receptor,ion regulation,salt absorption,ionocyte,medaka, | en |
| dc.relation.page | 42 | |
| dc.identifier.doi | 10.6342/NTU201901567 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2019-07-22 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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