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標題: | 太平洋雙色鰻於西太平洋之族群遺傳結構 Population Genetic Structure of the Anguilla bicolor pacifica in the West Pacific |
作者: | Yi-Ting Lai 賴奕廷 |
指導教授: | 韓玉山 |
關鍵字: | 太平洋雙色鰻,細胞色素c氧化?次單元I,族群遺傳結構,產卵場,北赤道洋流,民答那峨洋流,西里伯斯海, Anguilla bicolor pacifica,COI,population genetic structure,spawning area,North Equatorial Current (NEC),Mindanao Current (MC),Celebes Sea, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 太平洋雙色鰻為短鰭鰻,相關研究指出此物種可能為非隨機交配族群,然而由於對其族群遺傳結構所知甚少,到目前為止此觀點仍具有爭議。基於資源管理與進行保育工作的先決條件,探討此物種的族群遺傳結構具有十分重要的意義。為此,本篇研究分析來自西太平洋八個樣點,共192隻太平洋雙色鰻其玻璃鰻之遺傳變異,利用粒線體DNA COI基因發現45個單倍型,顯示低核苷酸歧異度(0.0023)與高單型多樣性(0.744),中性檢定為負值且顯著。以族群分化指數(FST)為距離係數,建構各族群之UPGMA樹狀圖得知太平洋雙色鰻在地理區域階級具分化現象,可分為(i)黑潮(Yilan及Aparri)與民答那峨洋流(Mati、General Santos及Dumoga River)一群和(ii)西里伯斯海(Pagadian、Zamboanga及Palu)一群。AMOVA分析結果表明,不同的地理區域之間存在顯著的遺傳分化。結果表明Pagadian、Zamboanga及Palu可劃分為同一保育區域,鰻苗產卵場與洋流漂送路徑的不同,可能是導致此族群遺傳結構的主要原因。本研究顯示,位於西太平洋之太平洋雙色鰻在地理區域階級具分化現象,建議按照不同的洋流系統分為兩個管理單位。 The short-finned eel, Anguilla bicolor pacifica, is considered to be non-panmictic. However, this view has been challenged because little is known about its population genetic structure. Resolving this issue is of high importance, as it is the prerequisite for conservation efforts. For this, we examined spatial and temporal genetic variation in the A. bicolor pacifica glass eels, collected from eight representative localities across the West Pacific. The partial cytochrome c oxidase subunit 1 (COI) mitochondrial DNA (mtDNA) gene was investigated in 192 specimens. A total of 45 haplotypes were detected. The genetic pattern revealed a low level of nucleotide diversity (0.0023) but a high level of haplotype diversity (0.744). Data showed the variability in populations and neutrality tests were significant. Based on the UPGMA tree of populations using pairwise FST as genetic distance measures in the West Pacific, the data were combined into (i) Kuroshio (Yilan and Aparri) and Mindanao Current (Mati, General Santos and Dumoga River) and (ii) Celebes Sea (Pagadian, Zamboanga and Palu) according to their sampling localities. Simulated scenarios of population genetic structure, tested with AMOVA resulted in significant values indicating the divergence between sampled groups according to different geographical regions. There was evidence of significant genetic differentiation among populations and a high correlation when the eel was divided into management units. The results suggested Pagadian, Zamboanga and Palu as separate conservation unit for A. bicolor pacifica in the West Pacific. The difference in spawning sites and larval dispersal routes may be invoked as the main determining factors for this population genetic structure. This study revealed that A. bicolor pacifica could be considered as two management units according to different ocean current systems. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21356 |
DOI: | 10.6342/NTU201901277 |
全文授權: | 未授權 |
顯示於系所單位: | 漁業科學研究所 |
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