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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 靳宗洛 | |
| dc.contributor.author | Yi-Hui Wang | en |
| dc.contributor.author | 王伊卉 | zh_TW |
| dc.date.accessioned | 2021-06-08T03:30:23Z | - |
| dc.date.copyright | 2019-08-18 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-08-14 | |
| dc.identifier.citation | Abreu IA, Cabelli DE (2010) Superoxide dismutases—a review of the metal-associated mechanistic variations. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1804: 263-274
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21291 | - |
| dc.description.abstract | 鐵作為許多重要蛋白質和酶的輔助因子是所有生物體中不可缺少的元素。金屬伴護蛋白質(Metallochaperones)透過直接的蛋白質間相互作用將金屬離子遞送給特定的生理夥伴。目前只有三個已被證實為鐵伴護蛋白質(Fe chaperones)，人類粒線體蛋白質Frataxin、細胞質的Poly(C)-binding protein及阿拉伯芥葉綠體伴護蛋白質Chaperonin 20。本研究找到四個阿拉伯芥中核醣核酸結合蛋白質(RNA binding proteins; RBPs)分別為RBP1, RBP2, GRP7, GRP8對於位在細胞質的鐵超氧歧化酶(ΔTP-FeSOD1)具有潛在的鐵伴護蛋白質功用。在先前的研究，藉由阿拉伯芥原生質體暫時性表現，我們發現這些核醣核酸結合蛋白質都具有活化細胞質鐵超氧歧化酶的能力。為了分析鐵超氧歧化酶與核醣核酸結合蛋白質之間的交互作用: 利用(1) 酵母菌雙雜合系統發現GRP7, GRP8會與鐵超氧歧化酶有交互作用；(2) 雙分子螢光互補實驗 (BiFC) 發現核醣核酸結合蛋白質於細胞質中都會與的鐵超氧歧化酶有交互作用。經由體外超氧歧化酶活性測試，當加入GRP7或 GRP8後可提升FSD1的活性。確認對細胞質型的鐵超氧歧化酶來說，核醣核酸結合蛋白質極有可能是其鐵伴護蛋白質，因此，認為核醣核酸結合蛋白質對於細胞質中需要鐵的蛋白質具有給予鐵的能力。 | zh_TW |
| dc.description.abstract | Iron (Fe) is an essential element in plants and animals, while excess Fe accumulation causes cytotoxic through the production of reactive oxygen species. Metallochaperones deliver ion cofactors to the specific physiological partners through direct protein-protein interactions. Only three Fe chaperones are known, i.e. human mitochondrial frataxin, cytoplasmic poly(C)-binding proteins, and Arabidopsis chloroplastic chaperonin 20. We identified four RNA binding proteins (RBPs) RBP1, RBP2, GRP7, and GRP8 which could be functioned as a Fe chaperone for cytoplasmic-destined Fe superoxide dismutase 1 (ΔTP-FSD1) activation in Arabidopsis (Arabidopsis thaliana). In this study, using yeast two-hybrid and bimolecular fluorescence complementation assay confirmed the interaction relationship between RBPs and FSD1. In vitro SOD activity assay showed that GRP7 and GRP8 enhanced the FSD1 activity. In conclusion, our results demonstrated a critical role for RBPs to be Fe chaperones for the cytosolic FeSOD activation in Arabidopsis. Our results also suggested that RBP plays a role in Fe metalation of cytoplasmic Fe-containing proteins. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T03:30:23Z (GMT). No. of bitstreams: 1 ntu-108-R05b42010-1.pdf: 3755568 bytes, checksum: 706e4247bf7e3bc621418ee0dd0ca339 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | ACKNOWLEDGEMENTS i
摘要 ii ABSTRACT iii TABLE OF CONTENTS iv LIST OF FIGURES viii LIST OF TABLES ix ABBREVIATIONS x INTRODUCTION 1 Metallochaperones 1 Fe chaperone and poly(C)-binding protein (PCBP) 1 RNA-binding proteins (RBPs) 3 Superoxide dismutases (SODs) and known metallochaperones for SODs activation 4 Motivation and objectives 5 MATERIALS AND METHODS 7 Plant materials and growth conditions 7 RNA extraction and reverse transcription PCR (RT-PCR) 8 Construction 8 Yeast two-hybrid assay 9 Subcellular localization and bimolecular fluorescence complementation (BiFC) assay 10 SOD activity assay and immunoblotting 11 Recombinant protein purification and holo-protein preparation 12 Primer and accession number 13 RESULTS 14 Cytoplasmic factor that assist the FeSOD1 (FSD1) activation 14 Candidate proteins as Fe metallochaperones for ΔTP-FSD1 activation 14 Cytoplasmic localization of RBP1, RBP2, GRP7, and GPP8 15 GRP7 and GRP8 facilitates the FSD1 Activity in vitro and in vivo 16 Characterization of GRP7 and GRP8 T-DNA insertion mutants 17 Analysis of the functional domain of GRP7 required for ΔTP-FSD1 activation 18 DISUSSION 19 RNA binding proteins are Fe chaperones for cytoplasmic-destined FSD (ΔTP-FSD) activation 19 Dual functions of GRP7 in the regulation RNA expression in nucleus and functions as an Fe chaperone in the cytosol 19 Interaction strength between GRP7, GRP8, and ΔTP-FSD1 20 Other factors in cytosol might involve in GRPs-FSD1 activation 20 Reporters for Fe chaperone activity 21 CONCLUSIONS AND PROSPECTS 24 TABLES 25 Table 1. Protein candidates identified by LC-MS/MS analysis. 25 Table 2. Primers used in this study. 26 FIGURES 30 Figure 1. The Arabidopsis FeSOD actives in Nicotiana benthamiana. 30 Figure 2. Subcellular localization of RNA binding proteins. 31 Figure 3. BiFC assay of ΔTP-FSD1 and RBPs. 32 Figure 4. ΔTP-FSD1 interacts with GRP7 and GRP8 in yeast two-hybrid assay. 33 Figure 5. Affinity purification of the GST and GST-tagged GRP7 and GRP8. 34 Figure 6. Affinity purification GST-FSD1 and its activity assay. 35 Figure 7. FSD1 activation by GRP7 and GRP8 in vitro. 37 Figure 8. Amino acid sequence alignment and domain truncation of GRP7 and GRP8 and. 38 Figure 9. Characterization of the T-DNA insertion line of GRP7 and GRP8 40 Figure 10. ΔTP-FSD1 activity in grp7-2 protoplasts. 41 Figure 11. Effect of the domain truncated GRP7 on ΔTP-FSD1 activity. 42 SUPPLEMENTAL FIGURES 43 Supplemental Figure S1. The Arabidopsis ΔTP-FeSOD1 (ΔTP-FSD1) is active in yeast Saccharomyces cerevisiae. 43 Supplemental Figure S2. Effect of RBPs on FSD1 activation. 44 Supplemental Figure S3. RBP1 significantly enhanced FSD1 activity in vitro. 45 APPENDIXES 46 REFERENCES 53 | |
| dc.language.iso | en | |
| dc.title | 阿拉伯芥核糖核酸結合蛋白GRP7和GRP8具活化細胞質鐵超氧歧化酶之功能性研究 | zh_TW |
| dc.title | RNA-binding Protein GRP7 and GRP8 are Capable for Cytoplasmic-destined FeSOD Activation in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 葉靖輝,張英?,張孟基,李昆達 | |
| dc.subject.keyword | 阿拉伯芥,鐵伴護蛋白質,鐵恆定,鐵超氧歧化?,富含甘胺酸蛋白質, | zh_TW |
| dc.subject.keyword | Arabidopsis thaliana,Fe chaperone,Fe homeostasis,Fe Superoxide dismutase (FeSOD),Glycine-rich proteins (GRPs), | en |
| dc.relation.page | 56 | |
| dc.identifier.doi | 10.6342/NTU201903321 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2019-08-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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