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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 鄭光成 | |
dc.contributor.author | Tai-Ching Kuo | en |
dc.contributor.author | 郭岱青 | zh_TW |
dc.date.accessioned | 2021-06-08T03:28:59Z | - |
dc.date.copyright | 2019-08-20 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-16 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21223 | - |
dc.description.abstract | 生質酒精具有取代石化燃料的潛能,屬於其中一種再生性能源。相較於利用糧食作物為原料,以農林業副產物作為原料所生產的木質纖維素酒精,可避免排擠糧食供應的疑慮。由於木質纖維素資材質地堅固,無法直接為微生物所發酵利用,故於發酵前需先經過預處理及水解步驟以破壞結構並釋放出可發酵糖。然而在此過程中可能生成部份不利後續酵母菌發酵的有毒抑制物,使得生質酒精產量降低。本研究以甘蔗渣作為原料,以 1.25%的硫酸水溶液進行混合,並於 121oC 的環境中進行 2 小時的預處理,待蔗渣烘乾後再以 2%的硫酸進行酸水解,此法所製備之蔗渣水解液在纖維素水解率上可達 62%。另外,此蔗渣水解液中可測得部份有毒抑制物,包含甲酸 0.94 g/L、乙酸 2.73 g/L、糠醛 0.36 g/L 及羥甲基糠醛 1.28 g/L,在常壓非熱電漿功率 200 W、25 分鐘處理時間的條件下可有效降解 31%甲酸、45%乙酸、100%糠醛及 80%羥甲基糠醛,順利降至酵母菌可耐受範圍。將電漿處理後之蔗渣水解液進行發酵,其酒精產率可自 0.25 g/L/h 提升至 0.65 g/L/h,大幅縮減生質酒精生產時程及成本。從本研究中顯示常壓非熱電漿具有降解木質纖維素水解液中有毒抑制物的能力,並可因此提高生質酒精的產量。我們期待此技術日後於再生能源領域中所能帶來的貢獻。 | zh_TW |
dc.description.abstract | Bioethanol offers a sustainable solution for transition from fossil-based fuels to renewable alternatives. The one produced from agricultural and forest residues, which is called lignocellulosic bioethanol, shows less conflicts with the food supply compared tothe one made from food crops. Since the recalcitrant structure of lignocellulosic material, the process of pretreatment and hydrolysis should be done prior to fermentation. However, toxic compounds that inhibit fermentation were also produced during the process, which reduce the productivity of bioethanol. In this study, sugarcane bagasse was chosen as lignocellulosic material and was first pretreated by 1.25% H2SO4 at 121oC for 2 hours. Later a diluted acid hydrolysis was conducted by using 2% H2SO4 before fermentation. The hydrolysis rate of the sugarcane hydrolysate could reach 62%. Within the hydrolysate, inhibitory compounds such as formic acid, acetic acid, furfural and hydroxymethylfurfural (HMF) were detected at the concentration of 0.94, 2.73, 0.36, 1.28 g/L, respectively. By applying atmospheric cold plasma for detoxification, 31% of formic acid, 45% of acetic acid, 80% of HMF and 100% of furfural were degraded under 200W treatment for 25 minutes, which the toxicity degree can be tolerated by fermenting yeast. While applied plasma-treated hydrolysate in fermentation, the ethanol productivity enhanced from 0.25 g/L/h to 0.65 g/L/h, which can considerably improve the efficiency in industry. Our results demonstrate that atmospheric cold plasma could effectively degrade the inhibitors within hydrolysate, therefore enhance the productivity of bioethanol. We anticipate the great potential of the technique in the field of renewable energy. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T03:28:59Z (GMT). No. of bitstreams: 1 ntu-108-R06642004-1.pdf: 3252691 bytes, checksum: 6862a182d45cb396a85ac256ddf72cd3 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 目錄
謝誌 i 中文摘要 ii Abstract iii 目錄 iv 圖目錄 ix List of tables xiv 一、前言 16 二、文獻回顧 18 2.1化石燃料介紹及其議題 18 2.1.1化石燃料種類 18 2.1.2環境及社會議題 18 2.2可再生性能源介紹 18 2.2.1再生能源種類 18 2.2.2生質能源 19 2.2.2.1生質柴油 19 2.2.2.2生質酒精 20 2.3利用不同原料發酵生產生質酒精 20 2.3.1利用糖質原料發酵生產 20 2.3.2利用澱粉質原料發酵生產 21 2.3.3利用木質纖維素原料發酵生產 22 2.3.3.1利用軟木發酵生產 24 2.3.3.2利用硬木發酵生產 24 2.3.4利用藻類原料發酵生產 25 2.4木質纖維素生質酒精預處理 25 2.4.1物理法 (Physical) 25 2.4.1.1輾壓法 (Milling) 25 2.4.1.2輻射法 (Irradiation) 26 2.4.1.3高溫水解法 (Hydrothermal) 27 2.4.1.4蒸氣爆裂法 (Steam explosion) 28 2.4.2物理化學法 (Physico-chemical) 28 2.4.2.1二氧化碳爆裂法 (CO2 explosion) 29 2.4.2.2氨水纖維爆裂法 (Ammonia fiber explosion, AFEX) 29 2.4.2.3濕式氧化法 (Wet oxidation) 29 2.4.3化學法 (Chemical) 30 2.4.3.1鹼處理法 (Alkaline treatment) 30 2.4.3.2酸處理法 (Acidic treatment) 30 2.4.3.3有機溶劑處理法 (Organic solvent) 31 2.4.4生物法 (Biological) 31 2.5預處理後抑制發酵之副產物 36 2.5.1糠醛 (Furfural) 36 2.5.2羥甲基糠醛 (Hydroxymethylfurfural) 37 2.5.3甲酸 (Formic acid)、乙酸 (Acetic acid) 37 2.5.4酚類化合物 (Phenolic compounds) 37 2.6水解抑制物之因應方式 39 2.6.1生質選擇 39 2.6.2物理法 39 2.6.3化學法 40 2.6.4生物分解 42 2.6.5酵母菌選擇或耐受性培養 42 2.6.6基因工程 43 2.7電漿介紹 48 2.7.1電漿概論 48 2.7.2常壓電漿的優勢 49 2.7.3電漿產生方式 49 2.7.4電漿氣體及特性 50 2.7.5電漿的應用 52 2.8生質酒精發酵方式 55 2.8.1批式發酵 (Batch fermentation) 55 2.8.2饋料-批式發酵 (Fed-batch fermentation) 55 2.8.3連續式發酵 (Continuous fermentation) 55 2.8.4非同步糖化式發酵 (Separate hydrolysis and fermentation, SHF) 55 2.8.5同步糖化式發酵 (Simultaneous saccharification fermentation, SSF) 56 2.9甘蔗概述 56 2.10發酵所使用之微生物 59 2.11 Kluyveromyces marxianus概述 60 三、研究目的與假設 61 3.1研究目的 61 3.2研究假設 61 3.3研究架構 61 四、材料與方法 63 4.1甘蔗渣預處理及水解 63 4.2發酵菌株挑選 63 4.3發酵培養基 63 4.4蔗渣農業資材 63 4.5替代氮源 63 4.6 HPLC標準品及分析溶劑 64 4.7儀器設備 64 4.8菌種保存 65 4.9甘蔗渣農業資材的前處理 66 4.10甘蔗渣預處理條件試驗 66 4.11甘蔗渣水解試驗 66 4.12 酵母菌發酵 67 4.13酵母菌檢量線製作 67 4.14電漿條件試驗 68 4.15分析 68 4.15.1樣品濃度分析 68 4.15.2 HPLC-RI條件 68 4.16統計 69 五、結果與討論 70 5.1蔗渣水解液製備 70 5.1.1預處理 70 5.1.2水解 72 5.2 Kluveromyces marxianus (K-21) 生化特性分析 74 5.2.1 K-21生長曲線 74 5.2.2 K-21對不同種碳源之利用及發酵情形 75 5.2.3 K-21對不同抑制物之耐受度試驗 78 5.3非熱電漿效果評估 92 5.3.1以處理時間為變因之電漿效果評估 92 5.3.2以電漿功率為變因之電漿效果評估 93 5.3.3目標物與降解率之關係探討 93 5.3.4電漿蔗渣水解液之處理結果 95 5.3.5電漿處理之蔗渣水解液發酵效果評估 97 5.4 替代氮源試驗 106 六、結論與展望 109 七、參考文獻 110 八、附錄 xv | |
dc.language.iso | zh-TW | |
dc.title | 常壓非熱電漿對蔗渣水解液進行解毒生產生質酒精 | zh_TW |
dc.title | Detoxification of Sugarcane Bagasse Hydrolysate by
Atmospheric Cold Plasma for Bioethanol Production | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉?睿,劉啟德,陳彥卉,邱致穎,丁俞文 | |
dc.subject.keyword | 木質纖維素,生質酒精,甘蔗渣,常溫電漿,解毒, | zh_TW |
dc.subject.keyword | lignocellulose,bioethanol,sugarcane bagasse,cold plasma,detoxification, | en |
dc.relation.page | 139 | |
dc.identifier.doi | 10.6342/NTU201903616 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2019-08-17 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
Appears in Collections: | 生物科技研究所 |
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ntu-108-1.pdf Restricted Access | 3.18 MB | Adobe PDF |
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