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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 嚴震東 | |
dc.contributor.author | Li-Wen Wang | en |
dc.contributor.author | 王立文 | zh_TW |
dc.date.accessioned | 2021-06-08T03:26:54Z | - |
dc.date.copyright | 2020-01-21 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-01-15 | |
dc.identifier.citation | Akopian AN, Sivilotti L & Wood JN. A tetrodotoxin-resistant voltage-gated sodium channel expressed by sensory neurons. Nature 1996, 379 (6562), 257–262.
Akopian AN, Souslova V, England S, Okuse K, Ogata N, Ure J, Smith A, Kerr BJ, McMahon SB, Boyce S, Hill R, Stanfa LC, Dickenson AH, Wood JN. The tetrodotoxin-resistant sodium channel SNS has a specialized function in pain pathway. Nature Neuroscience 1999, 2 (6), 541–548. Aniqa Tasnim, Zoe Rammelkamp, Amy B. Slusher, Krystyna Wozniak, Barbara S. Slusher and Mohamed H. Farah. Paclitaxel causes degeneration of both central and peripheral axon branches of dorsal root ganglia in mice. BMC Neuroscience 2016. Gary J. Bennett, Guo Kai Liu, Wen Hua Xiao, Hai Wei Jin1, and Chiang Siau. Terminal arbor degeneration (TAD): a novel lesion produced by the antineoplastic agent, paclitaxel. European Journal of Neuroscience 2011, 33 (9), 1667-1676. Katalin Jo´svay, Zolta´n Winter, Ro´bert L. Katona, La´szlo´ Pecze, Annama´ria Marton, Andrea Buhala, Gerda Szakonyi, Zolta´n Ola´h & Csaba Vizler. Besides neuro-imaging, the Thy1-YFP mouse could serve for visualizing experimental tumours, inflammation and wound-healing. Scientific Reports 2014, 4, 6776 Lisa A. Griffiths, Natalie A. Duggett, Ann L. Pitcher, and Sarah J. L. Flatters. Evoked and Ongoing Pain-Like Behaviours in a Rat Model of Paclitaxel-Induced Peripheral Neuropathy. Pain Research and Management 2018. Mary Ann Jordan, Leslie Wilson. Microtubules as a target for anticancer drugs. Nature Reviews Cancer 2004, 4 (4), 253-265 Miau-Hwa Ko, Ming-E Hub, Yu-Lin Hsieh, Chyn-Tair Lan, To-Jung Tseng Peptidergic intraepidermal nerve fibers in the skin contribute to the neuropathic pain in paclitaxel-induced peripheral neuropathy. Neuropeptides 2014, 48 (3), 109-117. Nathaniel T. Blair, Bruce P. Bean. Roles of tetrodotoxin (TTX)-sensitive Na+ current, TTX-resistant Na+ current, and Ca2+ current in the action potentials of nociceptive sensory neurons. The Journal of Neuroscience 2002, 22 (23), 10277-10290 Renata Zaja˛czkowska, Magdalena Kocot-Ke˛pska, Wojciech Leppert, Anna Wrzosek, Joanna Mika and Jerzy Wordliczek. Mechanisms of Chemotherapy-InducedPeripheral Neuropathy. International Journal of Molecular Sciences 2019 Renganathan M, Cummins TR, Waxman SG. Contribution of Nav1.8 Sodium Channels to Action Potential Electrogenesis in DRG Neurons. Journal of Neurophysiology 2001, 86 (2), 629-640. Robert J. Tanenberg. Diabetic Neuropathy: Painful or Painless. Hospital Physician 2009. Seretny M, Currie GL, Sena ES, Ramnarine S, Grant R, MacLeod MR, Colvin LA,and Fallon M. Incidence, prevalence, and predictors of chemotherapy-inducedperipheral neuropathy: A systematic review and meta-analysis. Pain 2014, 155 (12), 2461–2470. Shih-Tien Hsu, Chun-Hsu Yao, Yuan-Man Hsu, Jia-Horng Lin, Yung-Hsiang Chen & Yueh-Sheng Chen. Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model. Scientific Reports 2017. S. Lauren Kyte, Wisam Toma, Deniz Bagdas, Julie A. Meade, Lesley D. Schurman,Aron H. Lichtman, Zhi-Jian Chen, Egidio Del Fabbro, Xianjun Fang, John W. Bigbee, M. Imad Damaj, and David A. Gewirtz. Nicotine Prevents and Reverses Paclitaxel- Induced Mechanical Allodynia in a Mouse Model of CIPN. Journal of Pharmacology and Experimental Therapeutics 2017, 364, 110-119. S.R. Chaplan, F.W. Bach, J.W. Pogrel, J.M. Chung, T.L. Yaksh. Quantitative assessment of tactile allodynia in the rat paw. Journal of Neuroscience Methods 1994, 53 (1), 55-63. Wisam Toma, S. Lauren Kyte, Deniz Bagdas, Yasmin Alkhlaif, Shakir D. Alsharari, Aron H. Lichtman, Zhi-Jian Chen, Egidio Del Fabbro, John W. Bigbee , David A. Gewirtz , M. Imad Damaj. Effects of paclitaxel on the development of neuropathy and affective behaviors in the mouse. Neuropharmacology 2017, 305-315. Xiao-Long Zhang1, Xian-Ying Cao, Ren-Chun Lai1, Man-Xiu Xie and Wei-An Zeng. Puerarin Relieves Paclitael-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons. Frontiers in Pharmacology 2019 Yi-Fan Chen, Li-Hsien Chen, Yu-Min Yeh, Pei-Ying Wu, Yih-Fung Chen, Lian-Yun Chang, Jang-Yang Chang & Meng-Ru Shen. Minoxidil is a potential neuroprotective drug for paclitaxelinduced peripheral neuropathy. Scientific Reports 2017. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21093 | - |
dc.description.abstract | 化療所引起的周邊神經病變(Chemotherapy-induced peripheral neuropathy,CIPN)是紫杉醇治療的一種劑量限制性的副作用。紫杉醇的神經毒性可以導致神經纖維退化,這可能與動物在服用紫杉醇後的感受傷害行為有關。在我們的研究中,我們分別在第0、2、4和6天將紫杉醇以每公斤體重注射4毫克的劑量注射到基因轉殖小鼠的腹腔內,然後我們進行了一系列的實驗,例如von Frey測試和免疫組織化學染色。Thy 1-eGFP和Nav1.8-tdTomato小鼠分別被用來追蹤大的和小的皮下神經纖維。我們的實驗結果表明,注射紫杉醇的小鼠機械性閾值
(mechanical threshold)會降低,這是痛覺異常的徵兆。此外,我們追踪了紫杉醇注射後幾個時間點在活的小鼠中皮膚神經纖維的變化。大直徑的神經纖維未被發現有明顯變化。在機械性敏感化發生的時間點,Nav1.8-tdTomato小鼠的小直徑神經纖維和神經末端的密度有顯著的下降。我們得出的結論是,小纖維神經病變可能有助於CIPN的發生。 | zh_TW |
dc.description.abstract | Chemotherapy-induced peripheral neuropathy (CIPN) is a dose-limiting side effect of paclitaxel treatments. The neurotoxicity of paclitaxel can lead to the degeneration of nerve fibers, which may have a link with nociceptive behaviors in animals after paclitaxel administration. In our study, 4 mg/kg paclitaxel was injected intraperitoneally into transgenic mice on four alternate days (day 0, 2, 4 and 6), and then we conducted a series of experiments, such as von Frey assessment and immunohistochemistry. Thy 1-eGFP and Nav1.8-tdTomato reporter mice were used for tracking large and small subcutaneous nerve fibers respectively. Our results demonstrated that the paclitaxel-injected mice showed a reduced mechanical threshold, which was a sign of allodynia. Furthermore, we tracked the changes of cutaneous nerve fibers in living mice at several time points after paclitaxel injections. No large diameter fiber change was found. The densities of Nav1.8-tdTomato positive small diameter fibers and terminals significantly decreased at the time point at which mechanical sensitization occurred. We concluded that small fibers neuropathy may contribute to CIPN. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T03:26:54Z (GMT). No. of bitstreams: 1 ntu-109-R05B21011-1.pdf: 1291818 bytes, checksum: c0dab0a941a964b70daaf05f0a1ec9a4 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | Content…………………………………………………………………..2
摘要……………………………………………………………………....5 Abstract………………………………………………………………….6 Introduction …………………………………………………………….8 Paclitaxel and chemotherapy-induced peripheral neuropathy………………8 Nav1.8 and Thy 1………………………………………………………………10 Aim of this study……………………………………………………………….12 Materials and Methods………………………………………………..13 Animals…………………………………………………………………………13 Drugs…………………………………………………………………………...14 Administration of Paclitaxel…………………………………………………..15 Behavioral testing of mechanical allodynia………………………………….16 Immunohistochemistry………………………………………………………..17 In vivo longitudinal imaging with fluorescence microscope………………...18 In vivo longitudinal imaging with confocal microscope……………………..19 In vivo confocal microscopic image processing………………………………20 Statistical analysis……………………………………………………………..23 Results………………………………………………………………….24 Mice with paclitaxel injections developed mechanical hypersensitivity……24 Effects of paclitaxel on PGP9.5 intraepidermal nerve fiber innervation…..25 In vivo longitudinal effects of paclitaxel on large nerve fiber innervation in Thy-1 eGFP mice………………………………………………………………26 In vivo longitudinal effects of paclitaxel on small nerve fiber innervation in Nav1.8-tdTomato mice………………………………………………………...27 Discussion………………………………………………………………28 References………………………………………………………………30 Figures………………………………………………………………….34 Figure 1…………………………………………………………………………34 Figure 2…………………………………………………………………………35 Figure 3…………………………………………………………………………36 Figure 4…………………………………………………………………………37 Figure 5…………………………………………………………………………38 Figure 6…………………………………………………………………………39 Figure 7…………………………………………………………………………40 Figure 8…………………………………………………………………………41 Figure 9…………………………………………………………………………42 Figure 10………………………………………………………………………..43 Figure 11………………………………………………………………………..43 Figure 12………………………………………………………………………..44 Figure 13………………………………………………………………………..45 Figure 14………………………………………………………………………..46 | |
dc.language.iso | zh-TW | |
dc.title | 以光學方式觀察紫杉醇注射小鼠痛覺神經的變化 | zh_TW |
dc.title | Visualize Nociceptor Changes in Neuropathic Mice with Paclitaxel Injections | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳瑞芬,張鈞惠 | |
dc.subject.keyword | Nav1.8-Cre-tdTomato基因轉殖小鼠,紫杉醇,化療所引起的周邊神經病變,感受傷害行為,機械性的痛覺異常,表皮內的神經纖維,體內縱向成像, | zh_TW |
dc.subject.keyword | Nav1.8-Cre-tdTomato transgenic mice,paclitaxel,chemotherapy-induced peripheral neuropathy (CIPN),nociceptive behavior,mechanical allodynia,intraepidermal nerve fiber (IENF),in vivo longitudinal imaging, | en |
dc.relation.page | 46 | |
dc.identifier.doi | 10.6342/NTU202000131 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2020-01-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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