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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 施明哲(Ming-Che Shih) | |
dc.contributor.author | Hsiang Hsieh | en |
dc.contributor.author | 謝湘 | zh_TW |
dc.date.accessioned | 2021-06-08T03:06:49Z | - |
dc.date.copyright | 2017-07-07 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-06-30 | |
dc.identifier.citation | Ayano, M., Kani, T., Kojima, M., Sakakibara, H., Kitaoka, T., Kuroha, T., Angeles-Shim, R.B., Kitano, H., Nagai, K., and Ashikari, M. (2014). Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice. Plant Cell Environ. 37: 2313-2324.
Bailey-Serres, J., Fukao, T., Gibbs, D.J., Holdsworth, M.J., Lee, S.C., Licausi, F., Perata, P., Voesenek, L.A., and van Dongen, J.T. (2012). Making sense of low oxygen sensing. Trends in Plant Sci. 17: 129-138. Bailey-Serres, J., Fukao, T., Ronald, P., Ismail, A., Heuer, S., and Mackill, D. (2010). Submergence Tolerant Rice: SUB1’s Journey from Landrace to Modern Cultivar. Rice 3: 138-147. Banti, V., Giuntoli, B., Gonzali, S., Loreti, E., Magneschi, L., Novi, G., Paparelli, E., Parlanti, S., Pucciariello, C., Santaniello, A., and Perata, P. (2013). Low oxygen response mechanisms in green organisms. Int. J. Mol. Sci. 14: 4734-4761. Bui, L.T., Giuntoli, B., and Kosmacz, M. (2015). Constitutively expressed ERF-VII transcription factors redundantly. Plant Sci. 236: 37-43. Baena-Gonzalez, E., Rolland, F., Thevelein, J.M., and Sheen, J. (2007). A central integrator of transcription networks in plant stress and energy signaling. Nature 448: 938-942. Bailey-Serres, J., and Voesenek, L.A. (2008). Flooding stress: acclimations and genetic diversity. Annu. Rev. Plant Biol. 59: 313-339. Bhaskaran, U.P., and Varghese, T. (2009). Effect of submergence on alleviation of soil acidity and availability of nutrients in a rice-rice ecosystem. The Proceedings of the International Plant, Nutrition Colloquium XVI, UC Davis. Catling, D. (1992). The deepwater rice plants. Rice in deep water. (International Rice Research Institute), pp. 105-168. Cheong, Y.H. (2003). BWMK1, a Rice Mitogen-Activated Protein Kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor. Plant Physiol. 132: 1961-1972. Chen, S.Y., Chien, C.T., Chung, J.D., and Yang, Y.S. (2007). Dormancy-break and germination in seeds of Prunuscampanulata (Rosaceae): role of covering layers and changes in concentration of abscisic acid and gibberellins. Seed Sci. Res. 17: 21-32. Chen, P.W., Chiang, C.M., Tseng, T.H., and Yu, S.M. (2006). Interaction between rice MYBGA and the gibberellin response element controls tissue-specific sugar sensitivity of a-amylase genes. Plant Cell 18: 2326-2340. Chen, G., Hu, Q., Luo, L., Yang, T., Zhang, S., Hu, Y., Yu, L., and Xu, G. (2015). Rice potassium transporter OsHAK1 is essential for maintaining potassium-mediated growth and functions in salt tolerance over low and high potassium concentration ranges. Plant Cell Environ. 38: 2747-2765. Cho, H.T., and Kende, H. (1997). Expression of Expansin genes is correlated with growth in deepwater rice. Plant Cell 9: 1661-1671. De Angeli, A., Monachello, D., Ephritikhine, G., Frachisse1, J. M., Thomine1, S., Gambale, F., Barbier-Brygoo1, H. (2006). The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles. Nature 442: 939-942. FAO. (2003). Sustainable rice production for food security. In: Proceedings of the 20th session of the International Rice Commission. Fukao, T., and Bailey-Serres, J. (2008). Submergence tolerance conferred by Sub1A is mediated by SLR1 and SLRL1 restriction of gibberellin responses in rice. Proc. Natl. Acad. Sci. USA 105: 16814-16819. Fry, S.C., Smith, R.C., Renwick, K.F., Martin, D.J., Hodge, S.K., and Matthews, K.J. (1992). Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants. Biochem. J. 282: 821-828. Fukao, T., Xu, K., Ronald, P.C., and Bailey-Serres, J. (2006). A variable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice. Plant Cell 18: 2021-2034. Grennan, A.K. (2006). Gibberellin metabolism enzymes in rice. Plant Physiol. 141: 524-526. Gout, E., Bligny, R., and Douce, R. (1992). Regulation of intracellular pH values in higher plant cells. J. Biol. Chem. 267: 13903-13909. Giege, P., Heazlewood, J.L., Roessner-Tunali, U., Millar, A.H., Fernie, A.R., Leaver, C.J., and Sweetlove, L.J. (2003). Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis Cells. Plant Cell 15: 2140-2151. Gibbs, D.J., Lee, S.C., Isa, N.M., Gramuglia, S., Fukao, T., Bassel, G.W., Correia, C.S., Corbineau, F., Theodoulou, F.L., Bailey-Serres, J., and Holdsworth, M.J. (2011). Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants. Nature 479: 415-418. Gubler, F., Raventos, D., Keys, M., Watts, R., Mundy, J., and Jacobsen, J.V. (1999). Target genes and regulatory domains of the GAMYB transcriptional activator in cereal aleurone. The Plant Journal 17: 1–9. Harrison, M.A. (2012). Cross-talk between phytohormone signaling pathways under both optimal and stressful environmental conditions. Phytohormones and Abiotic Stress Tolerance in Plants. In N.A. Khan, R. Nazar, N. Iqubal, and N.A. Anjum, eds (Berlin/Heidelberg: Springer), pp. 49-76. Hebelstrup, K.H., Igamberdiev, A.U., and Hill, R.D. (2007). Metabolic effects of hemoglobin gene expression in plants. Gene 398: 86–93. Hunt, P.W., Klok, E.J., Trevaskis, B., Watts, R.A., Ellis, M.H., Peacock, W.J., and Dennis, E.S. (2002). Increased level of hemoglobin 1 enhances survival of hypoxic stress and promotes early growth in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 99: 17197-17202. Hattori, Y., Nagai, K., Furukawa, S., Song, X.J., Kawano, R., Sakakibara, H., Wu, J., Matsumoto, T., Yoshimura, A., Kitano, H., Matsuoka, M., Mori, H., and Ashikari, M. (2009). The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water. Nature 460: 1026-1030. Huang, J., Tang, D., Shen, Y., Qin, B., Hong, L., You, A., Li, M., Wang, X., Yu, H., Gu, M., and Cheng, Z. (2010). Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice. J. Genet. Genomics 37: 23-36. Igamberdiev, A.U., Baron, K.,Manach-Little, N., Stoimenova, M., and Hill, R.D. (2005). The haemoglobin/nitric oxide cycle: involvement in flooding stress and effects on hormone signalling. Ann. Bot. 96: 557–564. Izumi, K., Kamiya, Y., Sakurai, A., Oshio, H., and Takahashi, N. (1985). studies of sites of action of a new plant growth retardant (E)-l-(4-Chlorophenyl)- 4,4-dimethyl-2-(l,2,4-triazoI-l-yl)-l-penten-3-ol (S-3307) and comparative effects of its stereoisomers in a cell-free system from cucurbita maxima. Plant Cell Physiol. 26: 821-827. Itoh, H., Ueguchi-Tanaka, M., Sato, Y., Ashikari, M., and Matsuoka, M. (2002). The gibberellin signaling pathway is regulated by the appearance and disappearance of SLENDER RICE1 in nuclei. Plant Cell 14: 57-70. Ikeda, A., Ueguchi-Tanaka, M., Sonoda, Y., Kitano, H., Koshioka, M., Futsuhara, Y., Matsuoka, M., and Yamaguchi, J. (2001). slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. Plant Cell 13: 999–1010. Jung, K.H., Seo, Y.S., Walia, H., Cao, P., Fukao, T., Canlas, P.E., Amonpant, F., Bailey-Serres, J., and Ronald, P.C. (2010). The submergence tolerance regulator Sub1A mediates stress-responsive expression of AP2/ERF transcription factors. Plant Physiol. 152: 1674-1692. Jan, A., Yang, G., Nakamura, H., Ichikawa, H., Kitano, H., Matsuoka, M., Matsumoto, H., and Komatsu, S. (2004). Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice. Plant Physiol. 136: 3670-3681. Khush, G.S. (1984). Terminology for rice growing environments. (International Rice Research Institute), pp. 5-10. Koornneef , M., and van der Veen, J.H. (1980). Induction and Analysis of GibbereUin Sensitive Mutants in Arabidopsis thaliana (L.) Heynh. Theor. Appl. genet. 58: 257-263. Lee, Y., and Kende, H. (2001). Expression of beta-Expansins is correlated with internodal elongation in deepwater rice. Plant Physiol. 127: 645-654. Licausi, F., Kosmacz, M., Weits, D.A., Giuntoli, B., Giorgi, F.M., Voesenek, L.A., Perata, P., and van Dongen, J.T. (2011). Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization. Nature 479: 419-422. Magome, H., Nomura, T., Hanada, A., Takeda-Kamiya, N., Ohnishi, T., Shinma, Y., Katsumata, T., Kawaide, H., Kamiya, Y., and Yamaguchi, S. (2013). CYP714B1 and CYP714B2 encode gibberellin 13-oxidases that reduce gibberellin activity in rice. Proc. Natl. Acad. Sci. USA 110: 1947-1952. Mustroph, A., Zanetti, M.E., Jang, C.J.H., Holtan, H.E., Repetti, P.P., Galbraith, D.W., Girke, T., and Bailey-Serres, J. (2009). Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis. Proc. Natl. Acad. Sci. USA 106: 18843-18848. Nakayamaa, M., Koshioka, M., Matsuib, H., Ohara, H., Mander, L.N., Leitch, S.K., Twitchin, B., Kraft-Klaunzer, P., Pharis, R.P., and Yokota, T. (2001). Endogenous gibberellins in immature seeds of Prunus persica L.: identification of GA118,GA119,GA120,GA121,GA122 and GA126. Phytochemistry 57: 749–758. Nakano, T., Suzuki, K., Fujimura, T., and Shinshi, H. (2006). Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 140: 411-432. Nishitani, K., and Tominaga, R. (1992). Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. J. Biol. Chem. 29: 21058-21064. Ohwaki, Y., Kawagishi-Kobayashi, M., Wakasa, K., Fujihara, S., and Tadakatsu, Y. (2005). Induction of class-1 non-symbiotic hemoglobin genes by nitrate, nitrite and nitric oxide in cultured rice cells. Plant Cell Physiol. 46: 324-331. Ohme-Takagi, M., and Shinshi, H. (1995). Ethylene-lnducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell 7: 173-182. Rodriguez-Vargas, S., Sanchez-Garcia, A., Martinez-Rivas, J.M., Prieto, J.A., and Randez-Gil, F. (2007). Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress. Appl. Environ. Microbiol. 73: 110-116. Sun, T.P. (2011). The molecular mechanism and evolution of the GA-GID1-DELLA signaling module in plants. Curr. Biol. 21: R338-345. Sowa, A.W., Duff, S.M.G., Guy, P.A., and Hill, R.D. (1998). Altering hemoglobin levels changes energy status in maize cells under hypoxia. Proc Natl Acad Sci U S A 95: 10317–10321. Singh, N., Dang, T.T., Vergara, G.V., Pandey, D.M., Sanchez, D., Neeraja, C.N., Septiningsih, E.M., Mendioro, M., Tecson-Mendoza, E.M., Ismail, A.M., Mackill, D.J., and Heuer, S. (2010). Molecular marker survey and expression analyses of the rice submergence-tolerance gene SUB1A. Theor. Appl. Genet. 121: 1441-1453. Schmitz, A.J., Folsom, J.J., Jikamaru, Y., Ronald, P., and Walia, H. (2013). SUB1A-mediated submergence tolerance response in rice involves differential regulation of the brassinosteroid pathway. New Phytol. 198: 1060-1070. Shylaraj, K.S., and Sasidharan, N.K. (2005). VTL 5: A high yielding salinity tolerant rice variety for the coastal saline ecosystems of Kerala. J. Trop. Agr. 43: 25-28, 2005. Singh, P., and Sinha, A.K. (2016). A positive feedback loop governed by SUB1A1 interaction with MITOGEN-ACTIVATED PROTEIN KINASE3 imparts submergence tolerance in rice. Plant Cell 28: 1127–1143. Tsuji, H., Aya, K., Ueguchi-Tanaka, M., Shimada, Y., Nakazono, M., Watanabe, R., Nishizawa, N. K., Gomi, K., Shimada, A., Kitano, H., Ashikari, M., and Matsuoka, M. (2006). GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. Plant J. 47: 427-444. Tamang, B.G., and Fukao, T. (2015). Plant adaptation to multiple stresses during Submergence and following desubmergence. Int. J. Mol. Sci. 16, 30164-30180. Upchurch, R.G. (2008). Fatty acid unsaturation, mobilization, and regulation in the response of plants to stress. Biotechnol. Lett. 30: 967-977. Uozu, S., Tanaka-Ueguchi, M., Kitano, H., Hattori, K., and Matsuoka, M. (2000). Characterization of XET-related genes of rice. Plant Physiol. 122: 853-859. Vijayan, R. (2013). Pokkali rice cultivation in Kerala. Agriculture Update 11: 329-333. Vanaja, T., Neema, V.P., Mammootty, K.P., Rajeshkumar, R., Balakrishnan, P.C., Naik, J., and Raji, P. (2009). Development of first non-lodging and high-yielding rice cultures for saline kaipad paddy tracts of Kerala, India. Current Sci. 96: 1024-1028. Van de Poel, B., Smet, D., and Van Der Straeten, D. (2015). Ethylene and hormonal cross talk in vegetative growth and development. Plant Physiol. 169: 61-72. Wijayanti, L., Fujioka, S., Kobayashi, M., and Sakurai, A. (1996). Effect of uniconazole and gibberellin on the flowering of Pharbitis nil. Biosci. Biotech. Biochem. 60: 852-855. Wilson, R.N., Heckman, J.W., and Somerville, C.R. (1992). Plant Physiol. 100: 403-408. Warner, H.L., and Leopold, A.C. (1968). Ethylene evolution from 2-chloroethyl- phosphonic acid. Plant Physiol. 44: 156-158. Wang, K.L.C., Li, H., and Ecker, J.R. (2002). Ethylene biosynthesis and signaling networks. Plant Cell 14: S131-S151. Ward, J. M., Maser, P., and Schroeder, J. I. (2009). Plant ion channels: gene families, physiology, and functional genomics analyses. Annu. Rev. Physiol. 71: 59–82. Xu, K., and Mackill, D.J. (1996). A major locus for submergence tolerance mapped on rice chromosome 9. Mol. Breeding 2: 219-224. Xu, K., Xu, X., Fukao, T., Canlas, P., Maghirang-Rodriguez, R., Heuer, S., Ismail, A.M., Bailey-Serres, J., Ronald, P.C., and Mackill, D.J. (2006). Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature 442: 705-708. Yamaguchi, S. (2008). Gibberellin metabolism and its regulation. Annu. Rev. Plant Biol. 59: 225–51. Yang, S.F., and Hoffman, N.E. (1984). Ethylene biosynthesis and its regulation in higher plants. Ann. Rev. Plant Physiol. 35: 155-189. Yokoyama, R., Rose, J. K., and Nishitani, K. (2004). A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis. Plant Physiol. 3: 1088-1099. Zifarelli, G., and Pusch, M. (2010). CLC transport proteins in plants. FEBS Lett. 584: 2122-2127. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20851 | - |
dc.description.abstract | SUBMERGENCE1A-1 (SUB1A-1)是第七群乙烯反應轉錄因子(group-Vll ethylene response transcription factor, ERF-Vll)之一,在某些水稻品系的抗淹水逆境中扮演十分重要的角色,例如:FR13A和Pokkali。遭遇淹水時,SUB1A-1在FR13A中大量被誘導,且FR13A的生長會受到延遲,以利淹水期間節省能量消耗,直至水退後甫進行生長,此策略稱為low oxygen quiescence syndrome (LOQS)。雖然Pokkali水稻同樣具有SUB1A-1,但卻在淹水逆境中採取截然不同的策略。與FR13A相較之下,SUB1A-1的表現量在Pokkali中只受到淹水少量誘導,且Pokkali的生長並不會受到淹水的影響。從基因微陣列(microarray)分析結果發現,在淹水期間有一群負責將活化態GA轉換成非活化態的GA2 OXIDASE基因在Pokkali中有相對較低的誘導表現。另外,我們也發現GA下游的基因EXPANSIN在Pokkali中則有相對較高的誘導表現。進一步檢查在淹水期間三種水稻品系中活化態GA的變化情形,結果顯示,遭遇淹水後活化態GA會在Pokkali中逐漸累積,但在FR13A中並沒有明顯的變化。我們進一步發現大量的活化態GA使得SUB1A-1的表現受到抑制。另外,益收水溶液處理顯示,乙烯在淹水期間可能促進GA調控的下游反應,以利淹水期間植株得以持續生長。另一方面,我們也發現淹水逆境時,與FR13A相較之下,HEMOGLOBIN、ACYL-ACP DESATURASE、CLC7和HAK1基因在Pokkali中有較高的誘導表現。因此,根據實驗結果,我們推測淹水期間在Pokkali此種水稻品系中,乙烯促進GA調控的下游反應以及誘導少量的SUB1A-1表現,另一方面,大量累積的活化態GA進一步抑制了SUB1A-1的表現,使得SUB1A-1在Pokkali中維持較低的表現量。除此之外,我們也推測除了SUB1A-1之外,還有其他的因子在Pokkali的抗淹水逆境中參與了重要的調控。根據基因微陣列分析結果,我們找出可能調控Pokkali淹水耐受性的基因,包括了HEMOGLOBIN、ACYL-ACP DESATURASE、CLC7以及HAK1,這些基因尚需進一步的實驗加以驗證。
另一方面,先前研究指出同屬於第七群乙烯反應轉錄因子的ERF66和ERF67的表現量可能會受到SUB1A-1的調控。透過啟動子轉錄分析(trans-activation assay)實驗,我們發現SUB1A-1蛋白正向調控ERF66與ERF67的表現。另外,SUB1A-1基因本身的表現同樣也會受SUB1A-1蛋白的活化,有趣的是,此活性並不是透過SUB1A-1蛋白結合在SUB1A-1基因啟動子上已知的第七群乙烯反應轉錄因子結合序列GCC box,而是可能透過結合啟動子上其他的順式調控元素(cis-element)來調控其活化。根據以上實驗結果,我們推測在淹水期間,SUB1A-1蛋白透過活化本身SUB1A-1基因表現以及誘導下游ERF66與ERF67的表現量,來參與水稻抗淹水逆境的調控,給予水稻對於淹水的耐受性。 | zh_TW |
dc.description.abstract | SUBMERGENCE1A-1 (SUB1A-1) is a member of the group-Vll ethylene response transcription factors (ERF-Vlls) which confers major flooding-tolerance to some rice cultivars, including FR13A and Pokkali. Encountering submergence stress, FR13A shows a “low oxygen quiescence syndrome (LOQS)” phenotype, including: 1) the transcription level of SUB1A-1 is highly up-regulated and 2) the growth of FR13A is retarded until the water subsided. Although possessing SUB1A-1 as well, Pokkali displays a contrasting flooding-tolerant phenotype. Pokkali has a much lower induction level of SUB1A-1 than that of FR13A and continues to grow under submergence. Microarray data showed that some GA2 OXIDASEs encoding enzymes that catalyze active GA to inactive forms were lowly induced in Pokkali than in FR13A during submergence. Additionally, a series of EXPANSINs, which are GA-responsive genes, had much higher induction levels in Pokkali. GC/MS analysis indicated that the level of GA4 is highly increased in Pokkali under submergence, but not changed in FR13A. Furthermore, GA3 and ethephon treatments revealed that high levels of GA inhibited the expression of SUB1A-1 under submergence, and ethylene could promote GA response in rice, respectively. Moreover, a number of genes were highly induced in Pokkali than in FR13A under submergence, including HEMOGLOBIN, ACYL-ACP DESATURASE, CLC7 and HAK1, which may contribute to submergence tolerance of Pokkali. Based on these results, we hypothesized that in Pokkali ethylene triggers higher GA accumulation, which represses SUB1A-1 induction at lower levels. HEMOGLOBIN, ACYL-ACP DESATURASE, CLC7 and HAK1, which were highly induced in Pokkali than in FR13A under submergence, might participate in submergence tolerance of Pokkali.
Furthermore, prior studies showed that ERF66 and ERF67, members of ERF-Vlls, might be regulated by SUB1A-1. Through trans-activation assays, we proved that SUB1A-1 not only positively regulated the transcription of ERF66 and ERF67, but also activated itself at the transcription level. It is possible that SUB1A-1 binds other cis-element at the SUB1A-1’s promoter other than GCC box, which conferred flooding tolerance to rice by activating itself at the transcription level and enhanced the transcription of downstream targets, including ERF66 and ERF67. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T03:06:49Z (GMT). No. of bitstreams: 1 ntu-106-R04b42005-1.pdf: 3168156 bytes, checksum: c53e9e15c861c1e75caf0ba69aaf6b5e (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員議定書 ii
誌謝........... iv 摘要........... vi Abstract viii Table of contents x List of figures xiii List of tables xv Chapter 1 Introduction 1 Submergence poses a serious impact on crop yields 1 ERF-Vll group genes are involved in submergence tolerance 1 Low oxygen escape syndrome (LOES) in rice 2 Low oxygen quiescence syndrome (LOQS) in rice 3 SUB1A-1 is the key transcription factor for submergence tolerance 4 The submergence tolerance and characteristics of three rice cultivars 6 Material and Methods 8 Plant materials and growth condition 8 Submergence treatment and plant harvest 8 Ethephon treatment and plant harvest 9 RNA extraction 9 Reverse transcription 11 Quantitative RT-PCR 11 GA4 level measurement 12 Results and discussion 16 GA-related genes showed different expression patterns between FR13A and Pokkali 16 Gibberellins were involved in shoot elongation under submergence 17 GA4 level was significantly accumulated in Pokkali under submergence 19 GA could repress the expression of SUB1A-1 under submergence 20 Ethylene could promote the GA response in rice 22 Other factors might participate in submergence tolerance of Pokkali 25 Conclusions and future perspectives 31 Figures and tables 34 Chapter 2 Introduction 59 N-end rule pathway regulates the stability of some ERF-Vll proteins 59 SUB1A-1 could bind GCC box in the promoters of both ERF66 and ERF67 60 Material and Methods 62 Plant materials and growth condition 62 Rice protoplast isolation and transformation 62 LUC activity and GUS activity assay 64 Results and discussion 66 SUB1A-1 activated the transcription of both ERF66 and ERF67 66 SUB1A-1 positively regulated itself at transcription level 67 Conclusions and future perspectives 69 Figures and tables 70 References 76 | |
dc.language.iso | en | |
dc.title | 水稻品系Pokkali抗淹水逆境之調控機制 | zh_TW |
dc.title | Mechanism of Submergence Tolerance in Pokkali Rice | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 葉開溫(Kai-Wun Yeh),簡慶德(Ching-Te Chien) | |
dc.subject.keyword | 水稻,淹水耐受性,SUB1A-1,吉貝素,轉錄調控, | zh_TW |
dc.subject.keyword | gibberellin,rice,submergence tolerance,SUB1A-1,transcriptional regulation, | en |
dc.relation.page | 81 | |
dc.identifier.doi | 10.6342/NTU201700828 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2017-06-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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