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標題: | 阿拉伯芥富含半胱胺酸受體激酶18及絲裂原活化蛋白質激酶6在抗病反應抑制菜籽類固醇路徑及KottoiA/B胜肽透過不同受體調控植物氣孔和維管束之發育之功能性分析 Functional Characterization of CYSTEINE-RICH RECEPTOR-LIKE Arabidopsis PTI repression of BR signaling And KottoiA/B peptide regulates development of stomata and vasculature through distinct receptors |
作者: | Yi-Chun Huang 黃義竣 |
指導教授: | 金洛仁(Laurent Zimmerli) |
關鍵字: | 阿拉伯芥,免疫反應,賀爾蒙,生物逆境, arabidopsis,immunity,mpk6,pti, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 訊息胜肽調控植物的生長發育以及多種生理反應,如根部組織生長、葉形調控、防禦反應、癒合組織生長、頂端分生組織構型組成等。然而訊息胜肽如何調控植物組織之發育仍有許多未知,本研究透過比較植物在不同胜肽處理後之發育情形,篩選出阿拉伯芥CUE胜肽家族中的KottoiA/B胜肽,實驗顯示KottoiA/B會透過不同受體激酶調控葉部stomatal lineage stem cell數量以及根部之維管束發育。植物不僅能透過受體激酶感受內在訊號,也能藉由受體激酶接收環境訊息做出反應,本研究發現,富含半胱胺酸受體激酶18與絲裂原活化蛋白質激酶6能夠維持模式誘發免疫反應,使其不被菜籽類固醇訊號傳遞路徑抑制。透過對植物轉錄調控以及表型的分析發現,半胱胺酸重複受體激酶18及有絲分裂活化蛋白質激酶6會負向調控菜籽類固醇訊號傳遞路徑。因此推論半胱胺酸重複受體激酶18及有絲分裂活化蛋白質激酶6可能參與在模式誘發免疫反應抑制菜籽類固醇訊號傳遞路徑中。 Peptide signaling molecules are increasingly known to have important and diverse roles in regulation of developmental and physiological processes in plants. Here, we found that the peptide KottoiA/B, belonging to the CLAVATA3/ESR-unlike (CUE) family, regulates two different developmental processes, namely stomatal lineage and xylem developments in Arabidopsis thaliana. We also found that Kottoi A/B is perceived by two distinct receptor-like kinases (RLKs) systems in different tissues regulating distinct developmental processes. Plant cell use RLKs as transmembrane sensors to perceive and process peptide signals. RLKs not only sense endogenous signals but also exogenous signals. Our study revealed a novel RLK, CCR18 and it upstream MPK6 maintain the activation of PTI. Notably, by transcriptional and phenotype analyses, we show that CCR18 and MPK6 negatively regulate BRs signaling and positively regulate flg22-mediated growth inhibition. Therefore, CRK18 and MPK6 may be necessary for PTI-mediated reduction of BR-signaling. Our results further provide insight into understanding the fine-tune of plant immunity and may have potential for agricultural application. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20639 |
DOI: | 10.6342/NTU201702431 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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