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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳宏文 | |
dc.contributor.author | Chih-Kuan Yang | en |
dc.contributor.author | 楊子寬 | zh_TW |
dc.date.accessioned | 2021-06-08T02:53:49Z | - |
dc.date.copyright | 2017-08-25 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-09 | |
dc.identifier.citation | 1. Burton, G.J. and A.L. Fowden, The placenta: a multifaceted, transient organ. Philosophical Transactions of the Royal Society B: Biological Sciences, 2015. 370(1663): p. 20140066.
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PLOS Pathogens, 2010. 6(1): p. e1000732. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20575 | - |
dc.description.abstract | Serotonin (5-HT) mediates a variety of physiological activities through serotonin receptors, including regulation of peristaltic reflex in gastrointestinal tract, control of mood, appetite and sleep. In addition, maternal 5-HT plays a crucial role for fetal development. Therefore, the regulation of fetal access to maternal 5-HT becomes an important issue to study.
Serotonin transporter (SERT) has been reported to be responsible for the uptake of 5-HT and is highly expressed in placental tissues. In this study, we used ChIP-chip analysis to identify GCM1 as a SERT regulator. GCM1 is a placenta-specific transcription factor, regulating trophoblast differentiation and function during placentation. We found out that GCM1 will up-regulate the expression of SERT and enhance the 5-HT uptake in JAR cells. Furthermore, we discovered that SERT regulates the invasion of JAR and BeWo cells. Detection of SERT expression in chorionic villi and EVTs by immunohistochemical analysis suggested that SERT not only has a transport function in chorionic villi, but also involves in EVT invasion. In conclusion, we propose the regulation of SERT by GCM1 and a bifunctional role of SERT in 5-HT transport and trophoblast invasion. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T02:53:49Z (GMT). No. of bitstreams: 1 ntu-106-R04b46020-1.pdf: 3177877 bytes, checksum: b8d1e97c0875eec3c67610d9e09c183e (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員會審定書 i
Acknowledgement ii Contents iii 中文摘要 v Abstract vi Chapter 1. Introduction 1 1.1 Placenta 1 1.2 Serotonin 3 1.3 SERT 4 1.4 Preeclampsia 6 1.5 Trophoblast invasion 7 1.6 GCM1 8 1.7 Experimental rationales and specific aims 10 Chapter 2. Materials and Methods 11 2.1 Recombinant plasmid construction 11 2.2 Cell culture and transfection 14 2.3 Lentivirus infection 14 2.4 SDS polyacrylamide gel electrophoresis (PAGE) and western blotting 15 2.5 RNA extraction and quantitative PCR 16 2.6 Chromatin immunoprecipitation (ChIP) 17 2.7 Serotonin uptake assay 17 2.8 Luciferase reporter assay 18 2.9 Cell Invasion assay 18 Chapter 3. Results 20 Chapter 4. Discussion 24 Figures 28 Figure 1. GCM1 regulated SERT expression 29 Figure 2. GCM1 enhanced serotonin uptake in JAR cell 30 Figure 3. GCM1 transactivated SLC6A4 luciferase reporter construct 32 Figure 4. SERT promoted placental cell invasion 35 Figure 5. SSRI inhibited BeWo cell invasion 36 Appendix 37 Appendix 1. Placenta structures 37 Appendix 2. Trophoblast invasion in normal pregnancy and preeclampsia 39 Appendix 3. Identification of GCM1 target gene. 40 Appendix 4. Expression of SERT in human placenta. 41 Reference 42 | |
dc.language.iso | en | |
dc.title | 探討SERT的調控機制與其對胎盤細胞入侵能力的影響 | zh_TW |
dc.title | Regulation of SERT and its Implication in Placental Cell Invasion | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張震東,黃銓珍,張茂山,張功耀,黃娟娟 | |
dc.subject.keyword | 胎盤,SERT,GCM1,細胞入侵, | zh_TW |
dc.subject.keyword | placenta,SERT,GCM1,cell invasion, | en |
dc.relation.page | 52 | |
dc.identifier.doi | 10.6342/NTU201702841 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2017-08-10 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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