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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20425完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王淑慧(Shu-Huei Wang) | |
| dc.contributor.author | Ting-Hsin Chang | en |
| dc.contributor.author | 張庭欣 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:48:17Z | - |
| dc.date.copyright | 2017-09-08 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-18 | |
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Carcinogenesis , 32 (9), p.1372-1380. 6. Lee,T.K., Lee,D.K., Kim,D.I., Lee,Y.C., Chang,Y.C., Kim,C.H. (2004). Inhibitory effects of Scutellaria barbata D. Don on human uterine leiomyomal smooth muscle cell proliferation through cell cycle analysis. International Immunopharmacology , 4, p.447-454. 7. Jiang,Q.Q., Li,Q.Y., Chen,H.W., Shen,A., Cai,Q.Y., Lin,J.M.,& Peng,J. (2015). Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL‐6‐inducible STAT3 pathway activation in human colorectal cancer cells. EXPERIMENTAL AND THERAPEUTIC MEDICINE (10), p.1602-1608 . 8. Lin,W., Zheng,L.P., Zhuang,Q.C., Zhao,J.Y., Cao,Z.Y., Zheng,J.W., Lin,S., Xu,W.& Peng,J.(2013). Spica prunellae promotes cancer cell apoptosis, inhibits cell proliferation and tumor angiogenesis in a mouse model of colorectal cancer via suppression of stat3 pathway. Complementary&AlternativeMedicine, 13, p.144. 9. Xu,X.J., Zhao,L.L., Deng,C.Y., Yang,L., Wang, Y., Guo,T., Li,L.F., Lin,J.P.& Zhang L.R. 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Don polysaccharides inhibit the growth of Calu‐3 xenograft tumors via suppression of the HER2 pathway and angiogenesis . ONCOLOGY LETTERS , 9, p. 2721-2725. 15. Lai,K.G., Lin,Y.H., Ho,C.T., Chen,C.Y.,Peng,C.Y., Liu,T.Z. & Chiou,J.F. (2015). Paclitaxel pretreatment overcomes hypoxia inducible factor-1α-induced radioresistance acquisition of human hepatoma and lung adenocarcinoma cells. Life Science (136), p.7-12. 16. Prakobwong,S., Gupta,S.C., Kim,J.H., Sung,B., Pinlabor,P., Hiraku,Y., Wongkham,S., Sripa,B., Pinlaor,S. & Aggarwal,B.B. (2010). Curcumin suppresses proliferation and induces apoptosis in human biliary cancer cells through modulation of multiple cell signaling pathways. Carcinogenesis , 32 (9), p.1372-1380. 17. SiveenSikka,K.S. , Surana,R., Dai,X.Y., Zhang,J.W., Kumar,A.P., Tan,K.H., Sethi,G. & Bishayee,A. (2014). Targeting the STAT3 signaling pathway in cancer: Role of synthetic and natural inhibitors. Biochimica et Biophysica Acta, 1845, p.136-154. 18. Pan,L.T.,Sheung,Y., Guo,W.P., Rong,Z.B.& Cai,Z.M. (2016). Hedyotis diffusa plus Scutellaria barbata Induce Bladder Cancer Cell Apoptosis by Inhibiting Akt Signaling Pathway through Downregulating miR-155 Expression. Evidence-Based Complementary and Alternative Medicine , p.1-10. 19. Zhong,L.X., Zhang,Y., Wu, M.L., Liu,Y.N., Zhang,P., Chen,X.Y., Kong,Q.Y.,Liu,J. & Li,H. (2016). Resveratrol and STAT inhibitor enhance autophagy in ovarian cancer cells . Cell Death Discovery , 2, p.15071. 20. Suh,S.J., Yoon,J.W., Lee,T.K., Jin,U.H. & Kim,S.L.(2007). Chemoprevention of Scutellaria bardata on Human Cancer Cells and Tumorigenesis in Skin Cancer. Phytother. Res. , 21, p.135-141. 21. Chiu,W.H., Su,W.C., Li,C.L., Chen,C.L., Lin,C.F. (2015). An increase in glucosylceramide synthase induces Bcl-xL-mediated cell survival in vinorelbine-resistant lung adenocarcinoma cells. Oncotarget , 26 (4), p.20513-20524. 22. Chu,Y.W., Yang,P.C., Yang,S.C., Shyu,Y.C., Hendrix,M.J.C., Wu,R. & Wu,C.W. (1997). Selection of Invasive and Metastatic Subpopulations from a Human Lung Adenocarcinoma Cell Line. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 17, p.353-360. 23. Chen,Y.K., Wang,H.C., Ho,C.T., Chen,H.Y., Li,S., Chan,H.L., Chung,T.W., Tan,K.T., Li,Y.R., Lin,C.C. (2015). 5-Demethylnobiletin promotes the formation of polymerized tubulin,leads to G2/M phase arrest and induces autophagy via JNK activation in human lung cancer cell. Journal of Nutrtional Biochemistry , 26, p.484-504. 24. Lin,Y.C., Lin,Y.C., Shih,J.Y., Huang,W.J., Chao,S.W., Chang,Y.L. & Chen,C.C. (2015). DUSP1 Expression Induced by HDAC1 Inhibition Mediates Gefitinib Sensitivity in Non–Small Cell Lung Cancers. Clinical Cancer Research , 21, p.428-438. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20425 | - |
| dc.description.abstract | 癌症目前已躍居國人十大死亡原因之首，而肺癌也成為男女癌症死因之第一位。因此找出有效治療肺癌的方法，成為了當務之急。研究顯示半枝蓮（Scutellaria barbata D. Don; SB）具有清熱解毒及治療各種癌症腫瘤的作用。但其抗腫瘤作用機制尚不清楚。因此本篇實驗利用半枝蓮的提取物(SB)進行了體內抗腫瘤及體外對細胞毒性的實驗，以了解半枝蓮的抗癌作用及對生物體的功能影響。在體內實驗方面，我們將CL1-5人類肺腺癌細胞，皮下注射於BALB/c 裸鼠背側形成腫瘤，並給予腹腔注射SB來觀察在裸鼠身上移植性腫瘤的影響。在體外方面，觀察SB對於CL1-5人類肺腺癌細胞是透過什麼路徑來造成細胞毒性的影響。初步實驗結果顯示，在SB對於在裸鼠身上移植性腫瘤，發現有抑制腫瘤生長的趨勢，雖然無統計上顯著表現，但從外觀上可明顯判斷出差異。在體外實驗方面，SB 有效抑制CL1-5 細胞存活率，並且會增加細胞自噬的路徑相關蛋白質的表現，顯示SB 可能是藉由增加CL1-5 產生自噬，減少細胞的存活率。總結本篇實驗SB對於CL1-5人類肺腺癌細胞，不論在體內或體外實驗的確有抑制腫瘤細胞生長的效果，期望未來能夠成為在抗腫瘤方面的潛力藥物。 | zh_TW |
| dc.description.abstract | Lung cancer is most common type in Taiwan. Therefore, it is important to find an effective treatment of lung cancer. Several studies showed that scutellaria barbataD. Don(SB)has detoxification and anti-cancer effects. But the precise effects and mechanisms of SB on lung cancer was still unclear. The aim of thestudy was to evaluate the therapeutic effect and mechanismof SB on lung cancer in in vivoand in vitrostudy. In in vivoexperiments, SB showed a tendency to inhibit tumor growth in transplanted tumors in nude mice.In in vitroexperiments, SB reduced the CL1-5 cell viability in a dose dependent manner. And the cytotoxicityeffect was caused by autophagy. Inthis study, the use of SB for CL1-5 cells, which showed a positive effect in inhibiting the growth of tumor cells in nude mice in both in vivo and in vitro experiments, is hoped to become a future potential drug to treat lung cancer patients. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:48:17Z (GMT). No. of bitstreams: 1 ntu-106-R01446014-1.pdf: 1492551 bytes, checksum: 6d3a1027f76e8f609bf9987ebe2d32ac (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 口試委員會審定書........................................i
誌謝................................................. ii 目錄..................................................iii 中文摘要................................................iv Abstract................................................v 壹、緒論.................................................1 貳、實驗材料.............................................4 參、實驗方法.............................................8 肆、 實驗結果...........................................12 伍、 討論...............................................14 陸、 參考文獻...........................................23 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用細胞與動物模式探討半枝蓮對人類肺癌CL1-5 細
胞治療的效用 | zh_TW |
| dc.title | Scutellaria barbata D. Don induced autophagy in
human lung cancer cells in vivo and in vitro | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 龔秀妮,陳金銓 | |
| dc.subject.keyword | SB 半枝蓮萃取物,細胞自噬, | zh_TW |
| dc.subject.keyword | Scutellaria barbata D. Don (SB),autophagy, lung cancer, | en |
| dc.relation.page | 25 | |
| dc.identifier.doi | 10.6342/NTU201704024 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-08-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 解剖學暨細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 解剖學暨細胞生物學科所 | |
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