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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 沈弘俊(Horn-Jiunn Sheen) | |
| dc.contributor.author | Syu-Sheng Siao | en |
| dc.contributor.author | 蕭旭昇 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:46:54Z | - |
| dc.date.copyright | 2017-08-25 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-21 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20382 | - |
| dc.description.abstract | 本研究成功結合預濃縮機制(preconcentration mechanism)以及分離技術，將兩種步驟整合在一起，利用微影製程做出微米流道和Nafion作為離子選擇性薄膜(ion-selective membrane)，將上述兩者整合而成本實驗之檢測晶片，其中微米流道分為兩條，其一為濃縮流道(concentration channel)、其二為緩衝流道(buffer channel)而Nafion則橫跨兩流道。
預濃縮機制為施加一電位差於Nafion兩端，可產生濃度極化效應(ion concentration polarization, ICP)，意即一端為離子富集區(ion enrichment region)、另一端為離子空乏區(ion depletion region)，爾後在空乏區一側施加偏壓便可利用第二種電滲透流(electroosmosis of the second kind)使空乏區之高電壓側產生濃縮區塊(preconcentration plug)。 分離機制是藉由電泳(Electrophoresis)帶動，不同帶電量的螢光小球會有不同的速度，以及在突縮突擴流道中流場及電場造成的速度差，將不同粒徑的螢光小球進行分離。 | zh_TW |
| dc.description.abstract | In this thesis, we have successfully integrated preconcentration mechanism and electrophersis separation. Fabricate a detection chip by coupling microchannels which including two channels: concentration channel and buffer channel, and Nafion as ion selection membrane which crossing two channels.
Preconcentration mechanism is applying a voltage drop in Nafion ends at first, can produce ion concentration polarization (ICP) which refers to one of the end becomes ion enrichment and the other becomes ion depletion. Then we apply a bias voltage to generate electroosmosis of the second kind to aggregate the preconcentration plug near the ion depletion region on the high voltage side. The separation system is applying electrophoresis technique to make particles which have different net charge moving in different velocity. The particle velocity in flow field and electric field is affected by sudden contraction flow channel. Different size particles have different net charge. We can make different size particle separated in different velocity. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:46:54Z (GMT). No. of bitstreams: 1 ntu-106-R02543075-1.pdf: 4505367 bytes, checksum: 4e58813af53f78d386f874d092150e23 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 誌謝 i
摘要 ii ABSTRACT iii 目錄 iv 圖目錄 vi 表目錄 viii 第一章導論 1 1.1前言 1 1.2研究動機 1 1.3研究方法 2 1.4論文架構 2 第二章 文獻回顧 3 2.1生物晶片感測文獻回顧 3 2.1.1實驗室晶片 3 2.1.2免疫分析法(Immunoassay) 4 2.1.3樣本前處理的預濃縮技術 5 2.2奈米流體預濃縮晶片文獻回顧 6 2.3空乏區渦流文獻回顧 13 2.4電泳分離技術文獻回顧 16 第三章 實驗理論及技術背景 21 3.1奈米濃縮晶片之濃縮原理 21 3.1.1 電雙層 21 3.1.2 離子的區域性空乏與濃縮現象 24 3.1.3 預濃縮機制 (Mechanism of preconcentration) 27 3.2 Nafion®117 29 3.3 分離機制 30 3.3.1 電泳(Electrophoresis)及電滲流(Electroosmotic flow) 30 3.3.2 粒子流速分析 32 第四章 實驗設備與實驗方法 33 4.1 實驗架構 33 4.2 粒子控制晶片製程 34 4.2.1微米流道與製程 34 4.2.2 以Nafion®作為離子選擇性薄膜之塗布 36 4.2.3氧電漿接合製程 37 4.2 觀測系統實驗設備 38 第五章 實驗結果與討論 41 5.1濃縮實驗結果 41 5.1.1 預濃縮驗證 41 5.1.2 濃縮區塊與電泳之關係 42 5.1.3 壓力驅動流對濃縮區塊的影響 43 5.2分離實驗結果 47 5.2.1分離驗證 47 5.2.2流道對分離的影響 49 5.2.3電壓與流場之關係 50 5.2.4螢光小球與濃縮分離之關係 51 第六章 總結與未來展望 54 6.1 總結 54 6.2未來展望 55 參考文獻 56 | |
| dc.language.iso | zh-TW | |
| dc.title | 整合奈米流道預濃縮器與粒子分離技術用於粒子操作晶片 | zh_TW |
| dc.title | Integration of nanofluidic preconcentrator and particle separation for a particle controllable device | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 范育睿(Ray Yu-Jui Fan),盧彥文(Yen-Wen Lu) | |
| dc.subject.keyword | 奈米流體預濃縮,Nafion,分離,電泳, | zh_TW |
| dc.subject.keyword | Nanofluidic preconcentration,Nafion,Separation,Electrophoresis., | en |
| dc.relation.page | 59 | |
| dc.identifier.doi | 10.6342/NTU201703373 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-08-21 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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