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標題: | 重金屬對乳酸菌發酵臺灣赤楊生質物之影響 Impact of heavy metal on lactic acid fermentation from Alnus formosana biomass |
作者: | Ko-Yu Liu 劉科佑 |
指導教授: | 柯淳涵 |
關鍵字: | 臺灣赤楊,重金屬,乳酸蔨,酵素水解,乳酸, A.formosana,heavy metals,lactic acid bacteria,enzyme hydrolysis,lactic acid, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 植生復育為目前全球土壤與地下水污染整治頗受重視的整治工法,本研究為探討植生復育所生之生質物是否可做為永續再生材料使用,進而增加整治的環境效益。本研究以臺灣本土先驅樹種臺灣赤楊作為生質物,探討使用不同重金屬對以臺灣赤楊生質物生產乳酸之影響。本研究將經蒸氣爆碎之臺灣赤楊生質物,在不同重金屬含量下進行同時糖化與發酵。結果顯示,在1414.02 mg/L 的 Cr3+ 濃度之下發酵乳酸菌Lactobacillus casei與Lactobacillus acidophilus 所得結果為相對於未加入重金屬之控制組分別為 19.65 %與12.84 %的產率;在107.85 mg/L 的 Cd2+ 濃度之下以乳酸菌L. casei發酵可以得到相對於未加入重金屬之控制組達 109.93 %的產率,L. acidophilus發酵可以得到相對於未加入重金屬之控制組達 112.69 %的產率,為全部同時糖化與發酵實驗中最高之產率;在6832.65 mg/L的Pb2+ 濃度之下進行同時糖化發酵時乳酸菌L. acidophilus仍具有促進的效果,而相同濃度之下乳酸菌 L. casei則無顯著影響。本研究証實之含重金屬之先驅樹種臺灣赤楊生質物作為原料,可生產乳酸,因此本研究提供另一個植生復育所得生質物的利用方法。 Phytoremediation is highly regarded as an alternative method for soil and groundwater pollution remediation globally. However, there is a need to explore the application for biomass harvested from polluted sites after remediation. Formosan alder (Alnus formosana) is a fast-growing, adaptable pioneer native tree species in Taiwan, and it is particularly suitable for reforestation. In this study, steam exploded Formosan alder (Alnus formosana) biomass were employed to investigate lactic acid production by SSF (simultaneous saccharification and fermentation) under impacts of different heavy metals. Impacts of different heavy metals on saccharification were also investigated. Negative impacts were for SSF by Cr3+ demonstrated under 1414.02 mg Cr3+ /L: fermentation by Lactobacillus casei and L. acidophilus obtained results of 19.65% and 12.84% compared to blank controls. Positive impacts for SSF by Cd2+ were demonstrated under 107.85 mg Cd2+ /L: fermentation by L. casei and L. acidophilus obtained results of 109.93 % and 112.69 % compared to blank controls. No impacts for SSF by Pb2+ up to 6832.65 mg Pb2+/L was found for both Lactobacillus strains. This study demonstrates that SSF for production of lactic acid from Formosan alder biomass is able to tolerate a wide range of heavy metal concentration regimes. Hence, this study provides the alternative for using biomass harvested from phytoremediation sites. Such biomass can be used as sustainable regenerative biomaterial, thereby further enhance the benefits of environmental remediation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20266 |
DOI: | 10.6342/NTU201800038 |
全文授權: | 未授權 |
顯示於系所單位: | 森林環境暨資源學系 |
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