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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鍾國芳(Kuo-Fang Chung) | |
| dc.contributor.author | Yi-Hsuan Li | en |
| dc.contributor.author | 李宜軒 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:43:36Z | - |
| dc.date.copyright | 2018-02-23 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-01-25 | |
| dc.identifier.citation | 張至善. 2012 談樹皮布與構樹. 林業研究專訊, 19, 62-66.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/20264 | - |
| dc.description.abstract | 構樹（Broussonetia papyrifera (L.) L’Hér. ex Vent., paper mulberry）為太平洋南島語族重要物質文化樹皮布的主要製作原料，並極有可能隨著南島語族遷徙傳入太平洋，因此，透過研究構樹親緣地理關係，可推論南島語族遷徙路徑。由於過去的研究皆使用單一或少數分子標記作為討論依據，解析度低，因此本研究利用限制酶位點標定之核酸定序法（restriction-site associated DNA sequencing, RADSeq），利用Stacks軟體自137個個體間尋獲6,322個單一核酸多型性（single nucleotide polymorphism, SNP）位點，並進行最大似然性親緣關係分析，成功以全基因組範圍資料再次證實了太平洋構樹皆來自臺灣東南部，與南島語族遷徙相關的「出臺灣說（Out of Taiwan Hypothesis）」相符，且太平洋構樹個體間差異甚小，亦符合太平洋構樹的播遷皆是透過人為無性扦插繁殖之理論，利用此少數差異已可追蹤構樹於遠大洋洲的傳播路徑。過去研究中，以母系遺傳的葉綠體DNA片段作為分子標記，臺灣的構樹基因型明顯具三區地理結構。而本研究所使用之分子標記主要分布於核基因體中，可由花粉攜帶傳播，結果顯示構樹花粉傳播能力強，能將核基因傳播甚遠，造成原發現臺灣島內之地理結構不呈現於新分析結果中，但即便如此，臺灣海峽仍能形成一有效屏障，隔離中國大陸與臺灣族群。從RADSeq分析結果中，亦發現琉球群島族群與臺灣東部族群最為相近，但仍有相當分化，可能是因為第四紀時曾有兩次冰河期陸橋連接，之後又因海平面上升造成族群長期隔離所致。 | zh_TW |
| dc.description.abstract | Paper mulberry (Broussonetia papyrifera (L.) L’Hér. ex Vent.), a wind-pollinated dioecious tree native to East Asia, is the cultural necessities of Austronesian-speaking peoples (Austronesians) for making bark cloth (tapa). By studying the geographical relationship among the populations of paper mulberry, the migration path of the Austronesians can be deduced. To increase phylogenetic resolution of previous studies limited by insufficient molecular resolution, we applied restriction-site associated DNA sequencing (RADSeq) collected using next-generation sequencing technology, obtaining 6,322 SNPs from 137 individuals for phylogenetic analysis by Stacks. The outcome reaffirmed that the Pacific paper mulberries were all derived from southeastern Taiwan, and the genetic differences among the Pacific paper mulberry were rare, conforming asexual propagation of the Pacific paper mulberry for tapa making and reaffirming the “out of Taiwan” hypothesis of Austronesian expansion and migration. Tough the genetic differences were rare, we still succeeded in finding the propagation path of paper mulberry in remote Oceania. The previous study, which using the chloroplast marker, showed the strong phylogeographic structure in Taiwan. On the contrary, the lack of phylogeographic structure in Taiwan revealed by RADSeq, which represents the biparental-inherited nuclear genome, suggests high gene flow mediate by efficient pollen dispersal. Nevertheless, Taiwan Strait remains an effective dispersal barrier for pollen dispersal between mainland China and Taiwan. Our RADSeq data also confirm the tight relationship between populations of Ryukyu Islands and eastern Taiwan. However, substantial genetic differentiation is present between the two populations, suggesting a long-term isolation and that the Ryukyus populations were likely established via land bridge during the twice ice age. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:43:36Z (GMT). No. of bitstreams: 1 ntu-107-R02625049-1.pdf: 9618674 bytes, checksum: 72af17f46ea7f98154e16c98b5bf801b (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 圖目錄 & 表目錄III
一、前言1 （一）構樹與樹皮布文化1 （二）南島語族遷徙假說2 （三）利用構樹推測南島語族文化的起源與傳播5 （四）次世代定序與限制酶位點標定之核酸定序法9 （五）研究目標11 二、材料與方法12 （一）取樣12 （二）DNA萃取、PCR產物定序與葉綠體基因單型鑑定15 （三）RAD 定序庫建構16 （四）RADSeq資料處理22 （五）親緣關係分析23 三、結果24 （一）中國大陸族群與臺灣族群分隔27 （二）臺灣族群之個體無分群27 （三）太平洋島嶼個體是從臺灣東南部族群延伸出之長枝單系群，且個體變異小27 （四）琉球群島族群是從臺灣東部族群延伸出之長枝單系群28 （五）日本、夏威夷 (基因單型cp-41) 個體皆來自中國大陸28 （六）太平洋小島個體分群29 四、討論30 （一）以構樹親緣地理關係推論南島語族遷徙假說30 （二）臺灣島內構樹地理結構33 （三）臺灣北部與中國福建、廣東沿海cp-01構樹之關係36 （四）琉球與臺灣東部構樹族群之關係36 （五）日本與夏威夷 (cp-41) 構樹來源38 （六）RADSeq資料分析問題39 五、結論41 參考文獻42 附錄：構樹採集資訊一覽表50 | |
| dc.language.iso | zh-TW | |
| dc.title | 以限制酶位點標定之核酸定序法檢視太平洋構樹之親緣地理 | zh_TW |
| dc.title | Reconstructing Phylogeography of Pacific Paper Mulberry using RADSeq | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 陳凱儀(Kai-Yi Chen) | |
| dc.contributor.oralexamcommittee | 胡哲明(Jer-Ming Hu),王俊能(Chun-Neng Wang) | |
| dc.subject.keyword | 南島語族遷徙,共生物種,次世代定序,Stacks,出臺灣說,琉球群島,冰河陸橋, | zh_TW |
| dc.subject.keyword | Austronesian expansion and migration,commensal species,next-generation sequencing (NGS),Stacks,Out of Taiwan hypothesis,Ryukyu Islands,ice age,land bridge, | en |
| dc.relation.page | 55 | |
| dc.identifier.doi | 10.6342/NTU201800168 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2018-01-25 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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