請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19926
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 盧彥文(Yen-Wen Lu) | |
dc.contributor.author | Yun-Yen Chiu | en |
dc.contributor.author | 邱勻彥 | zh_TW |
dc.date.accessioned | 2021-06-08T02:27:32Z | - |
dc.date.copyright | 2015-08-20 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-17 | |
dc.identifier.citation | Bhagat, A. A., S. S. Kuntaegowdanahalli and I. Papautsky, 2008, 'Continuous particle separation in spiral microchannels using Dean flows and differential migration', 8, 1906-1914.
Bianchi, D. W., G. K. Zickwolf, M. C. Yih and A. F. Flint, 1993, 'Erythroid-specific antibodies enhance detection of fetal nucleated erythrocytes in maternal blood', 13, 293-300. Chen, D. F., H. Du and W. H. Li, 2006, 'A 3D paired microelectrode array for accumulation and separation of microparticles', 16, 1162-1169. Chen, X., D. Cui, C. Liu, H. Li and J. Chen, 2007, 'Continuous flow microfluidic device for cell separation, cell lysis and DNA purification', 584, 237-243. Chiou, P. Y., A. T. Ohta and M. C. Wu, 2005, 'Massively parallel manipulation of single cells and microparticles using optical images', 436, 370-372. Choi, S. and J. K. Park, 2007, 'Continuous hydrophoretic separation and sizing of microparticles using slanted obstacles in a microchannel', 7, 890-897. Choi, S., S. Song, C. Choi and J. K. Park, 2007, 'Continuous blood cell separation by hydrophoretic filtration', 7, 1532-1538. Choi, S., S. Song, C. Choi and J. K. Park, 2009, 'Microfluidic self-sorting of mammalian cells to achieve cell cycle synchrony by hydrophoresis', 81, 1964-1968. Cummings, E. B. and A. K. Singh, 2003, 'Dielectrophoresis in microchips containing arrays of insulating posts: Theoretical and experimental results', 75, 4724-4731. Cunniff, C., 2004, 'Prenatal screening and diagnosis for pediatricians', 114, 889-894. Di Carlo, D., D. Irimia, R. G. Tompkins and M. Toner, 2007, 'Continuous inertial focusing, ordering, and separation of particles in microchannels', 104, 18892-18897. Einstein, A., 1905, 'Eine neue Bestimmung der Moleküldimensionen', Furlani, E. P., 2007, 'Magnetophoretic separation of blood cells at the microscale', 40, 1313-1319. Gossett, D. R., W. M. Weaver, A. J. Mach, S. C. Hur, H. T. Tse, W. Lee, H. Amini and D. Di Carlo, 2010, 'Label-free cell separation and sorting in microfluidic systems', 397, 3249-3267. Han, K.-H. and A. Bruno Frazier, 2004, 'Continuous magnetophoretic separation of blood cells in microdevice format', 96, 5797. Honda, H., N. Miharu, Y. Ohashi, O. Samura, M. Kinutani, T. Hara and K. Ohama, 2002, 'Fetal gender determination in early pregnancy through qualitative and quantitative analysis of fetal DNA in maternal serum', 110, 75-79. Hou, H. W., A. A. S. Bhagat, W. C. Lee, S. Huang, J. Han and C. T. Lim, 2011, 'Microfluidic Devices for Blood Fractionation', 2, 319-343. Hsu, C. H., D. Di Carlo, C. Chen, D. Irimia and M. Toner, 2008, 'Microvortex for focusing, guiding and sorting of particles', 8, 2128-2134. Huang, L. R., E. C. Cox, R. H. Austin and J. C. Sturm, 2004, 'Continuous Particle Separation Through Deterministic Lateral Displacement', 304, 987. Huang, R., T. A. Barber, M. A. Schmidt, R. G. Tompkins, M. Toner, D. W. Bianchi, R. Kapur and W. L. Flejter, 2008, 'A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women', 28, 892-899. Hughes, M. P., R. Pethig and X. B. Wang, 1996, 'Dielectrophoretic forces on particles in travelling electric fields', 29, 474-482. Ji, H. M., V. Samper, Y. Chen, C. K. Heng, T. M. Lim and L. Yobas, 2008, 'Silicon-based microfilters for whole blood cell separation', 10, 251-257. Jung, J. and K.-H. Han, 2008, 'Lateral-driven continuous magnetophoretic separation of blood cells', 93, 223902. Kantak, C., C. P. Chang, C. C. Wong, A. Mahyuddin, M. Choolani and A. Rahman, 2014, 'Lab-on-a-chip technology: impacting non-invasive prenatal diagnostics (NIPD) through miniaturisation', 14, 841-854. Kavanagh, D. M., M. Kersaudy-Kerhoas, R. S. Dhariwal and M. P. Desmulliez, 2010, 'Current and emerging techniques of fetal cell separation from maternal blood', 878, 1905-1911. Kim, M., S. Mo Jung, K. H. Lee, Y. Jun Kang and S. Yang, 2010, 'A microfluidic device for continuous white blood cell separation and lysis from whole blood', 34, 996-1002. Kuo, J. S., Y. Zhao, P. G. Schiro, L. Ng, D. S. Lim, J. P. Shelby and D. T. Chiu, 2010, 'Deformability considerations in filtration of biological cells', 10, 837-842. Lee, D., P. Sukumar, A. Mahyuddin, M. Choolani and G. Xu, 2010, 'Separation of model mixtures of epsilon-globin positive fetal nucleated red blood cells and anucleate erythrocytes using a microfluidic device', 1217, 1862-1866. Li, X., W. Chen, G. Liu, W. Lu and J. Fu, 2014, 'Continuous-flow microfluidic blood cell sorting for unprocessed whole blood using surface-micromachined microfiltration membranes', 14, 2565-2575. Lo, Y. M., N. Corbetta, P. F. Chamberlain, V. Rai, I. L. Sargent, C. W. Redman and J. S. Wainscoat, 1997, 'Presence of fetal DNA in maternal plasma and serum', 350, 485-487. Lo, Y. M., P. Patel, J. S. Wainscoat, M. Sampietro, M. D. Gillmer and K. A. Fleming, 1989, 'Prenatal sex determination by DNA amplification from maternal peripheral blood', 2, 1363-1365. Lo, Y. M., M. S. Tein, T. K. Lau, C. J. Haines, T. N. Leung, P. M. Poon, J. S. Wainscoat, P. J. Johnson, A. M. Chang and N. M. Hjelm, 1998, 'Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis', 62, 768-775. Mohamed, H., J. N. Turner and M. Caggana, 2007, 'Biochip for separating fetal cells from maternal circulation', 1162, 187-192. Morijiri, T., S. Sunahiro, M. Senaha, M. Yamada and M. Seki, 2011, 'Sedimentation pinched-flow fractionation for size- and density-based particle sorting in microchannels', 11, 105-110. Nagrath, S., L. V. Sequist, S. Maheswaran, D. W. Bell, D. Irimia, L. Ulkus, M. R. Smith, E. L. Kwak, S. Digumarthy, A. Muzikansky, P. Ryan, U. J. Balis, R. G. Tompkins, D. A. Haber and M. Toner, 2007, 'Isolation of rare circulating tumour cells in cancer patients by microchip technology', 450, 1235-1239. Pergament, E., J. D. Schulman, K. Copeland, B. Fine, S. H. Black, N. A. Ginsberg, M. C. Frederiksen and R. J. Carpenter, 1992, 'The risk and efficacy of chorionic villus sampling in multiple gestations', 12, 377-384. Purwosunu, Y., A. Sekizawa, K. Koide, S. Okazaki, A. Farina and T. Okai, 2006, 'Clinical potential for noninvasive prenatal diagnosis through detection of fetal cells in maternal blood', 45, Purwosunu, Y., A. Sekizawa, K. Koide, S. Okazaki, A. Farina and T. Okai, 2006, 'Clinical potential for noninvasive prenatal diagnosis through detection of fetal cells in maternal blood', 45, 10-20. Roscoe, R., 1952, 'The viscosity of suspensions of rigid spheres', 3, Safarık, I. and M. Safarıkova, 1999, 'Use of magnetic techniques for the isolation of cells', 722, 33–53. Sajeesh, P. and A. K. Sen, 2013, 'Particle separation and sorting in microfluidic devices: a review', 17, 1-52. Samura, O., A. Sekizawa, D. K. Zhen, V. M. Falco and D. W. Bianchi, 2000, 'Comparison of fetal cell recovery from maternal blood using a high density gradient for the initial separation step:1.090 versus 1.119 g/ml', 20, 281-286. Sethu, P., A. Sin and M. Toner, 2006, 'Microfluidic diffusive filter for apheresis (leukapheresis)', 6, 83-89. Shulman, L. P. and S. Elias, 1993, 'Amniocentesis and Chorionic Villus Sampling', 159, 260. Simha, R., 1940, 'The Influence of Brownian Movement on the Viscosity of Solutions.', 44, Simpson, J. L. and S. Elias, 1993, 'Isolating Fetal Cells From Maternal Blood', 270, 19. Takagi, J., M. Yamada, M. Yasuda and M. Seki, 2005, 'Continuous particle separation in a microchannel having asymmetrically arranged multiple branches', 5, 778-784. Wang, S., H. Wang, J. Jiao, K. J. Chen, G. E. Owens, K. Kamei, J. Sun, D. J. Sherman, C. P. Behrenbruch, H. Wu and H. R. Tseng, 2009, 'Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells', 48, 8970-8973. Winichagoon, P., S. Sithongdee, S. Kanokpongsakdi, P. Tantisirin, L. F. Bernini and S. Fucharoen, 2005, 'Noninvasive Prenatal Diagnosis for Hemoglobin Bart's Hydrops Fetalis', 81, 396-399. Xu, G., M. B. Chan, C. Yang, P. Sukumar, M. Choolani and J. Y. Ying, 2006, 'Design and Fabrication a Microfluidic Device for Fetal Cells Dielectrophoretic Properties Characterization', 34, 1106-1111. Yamada, M. and M. Seki, 2005, 'Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics', 5, 1233-1239. Zheng, S., H. Lin, J. Q. Liu, M. Balic, R. Datar, R. J. Cote and Y. C. Tai, 2007, 'Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells', 1162, 154-161. Zheng, Y. L., N. P. Carter, C. M. Price, S. M. Colman, P. J. Milton, G. A. Hackett, M. F. Greaves and M. A. Ferguson-Smith, 1993, 'Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting', 30, 1051-1056. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19926 | - |
dc.description.abstract | 細胞分離是血液分析過程中的一個重要步驟。主要應用在血液的樣本中萃
取出目標細胞,以進行後端的檢測分析,常被應用在分離出循環癌症細胞 (Circulating Tumor Cells, or CTC)來研究癌症擴散的情形,或是從母體的血液中 分離出其胎兒的有核紅血球(fetal Nucleated Red Blood Cells, or fNRBC) 來進行 產前檢測。近年來隨著生物晶片(Lab-on-a-Chip) 快速發展,藉由製造出更接近 細胞尺度的裝置,微流道晶片被有效應用在細胞分離上,可以達到更有效且敏 感的細胞分離結果。 一般而言,血液中帶核的細胞大小都在8 μm 以上,而紅血球為雙凹扁平狀, 厚度約為2~3 μm,本研究目標在利用細胞在尺寸上的差異,來分離血液中有核 紅血球(fNRBCs)。實驗設計則是利用橫向流(cross-flow) 的篩選原理,在側邊 加上額外的流體(buffer),產生流體聚集(hydrodynamic focusing)的效果,而將 帶有細胞的樣本流體(sample flow)推向篩選流道(filters),無核較小的細胞會通 過篩子而收集後被移除,有核較大的細胞則無法通過篩子,沿著主要流道後被 收集。此機制,可以充分利用橫向流的好處來減少細胞在流道內的阻篩,再配合 流體集中的效果增加過濾的壓力、提升分離效率。 在本論文裏,首先討論了實驗原理與設計,接著利用模擬分析了解流道的 設計和原理,然後是微流道晶片製作以及進行利用微流道來分離不同樣本的實 驗。實驗分為兩個階段,第一階段,先用2.7 μm 和10.6 μm 大小不同的微珠, 模擬細胞的分離情況,驗證設計在分離尺寸上差異的微珠可行性。藉由最佳的 流速結果,再進行細胞的分離,比較裝置設計對含量稀少的細胞收集的靈敏度。 研究結果比較不同流速下對細胞分離、回收效率和純度的影響。本研究利用設 計的微流道晶片,成功分離不同尺寸的微珠和細胞,並應用在血液的分離。 關鍵詞: 微流道,非侵入式檢測,有核紅血球,細胞分離,cross-flow | zh_TW |
dc.description.abstract | A microfluidic chip is proposed to separate particles using cross-flow filtration enhanced with hydrodynamic focusing. By exploiting a buffer flow from side, the particles in the sample flow are pushed on one side of the microchannels, while a larger pressure gradient in the filters is obtained to enhance separation efficiency. Our proposed mechanism has the buffer flow to create a moving virtual boundary for the sample flow to actively push all the particles to reach the filters for separation. This filtration device only requires soft lithograph fabrication to create microchannels and a novel pressurized bonding technique to make high-aspect-ratio (HAR) filtration structures. A mixture of polystyrene particles with 2.7 μm and 10.6 μm diameters are successfully separated. 96.2 ± 2.8% of the large particle are recovered with a purity of 97.9 ± 0.5%, while 97.5 ± 0.4% of the small particle are depleted with a purity of 99.2 ± 0.4% at a throughput of 10 μl/min. The experiment is also conducted with the sample solutions of spiked PC3 cells in whole blood, whole blood sample and cord blood sample. Our device offered a label-free and high separation efficiency technique with simple fabrication and integrable possibility with other components as a promising tool for continuous cell filtration and analysis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T02:27:32Z (GMT). No. of bitstreams: 1 ntu-104-R02631029-1.pdf: 3058813 bytes, checksum: 969d367ed35268922b601541a671f37e (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii List of Figures vii List of Tables ix Chapter 1 Introduction 1 1.1 Prenatal Diagnosis 1 1.2 Non-Invasive Prenatal Diagnostics (NIPD) 3 1.3 Overall Structure of Thesis 5 Chapter 2 Literature Review 7 2.1 Blood Cell Separation Using Microfluidics 7 2.1.1 Blood Cells 7 2.1.2 Microfluidic Cell Separation and Sorting 8 2.1.3 Pinched Flow Fractionation 10 2.1.4 Physical Filtration 12 2.2 Fetal Nucleated Red Blood Cells Separation 17 2.2.1 Current Techniques for fNRBCs Separation 17 2.2.2 Microfluidic Approaches to Isolate fNRBCs 19 Chapter 3 Materials and Methods 22 3.1 Microchip Design 22 3.2 Separation principle 24 3.2.1 Flow Resistance at Fixed Boundary 24 3.2.2 Flow Resistance in Hydrodynamic Focusing 27 3.3 Device Fabrication 29 3.3.1 Lithography on Silicon Wafer 29 3.3.2 Pressure Bonding Technic on PDMS Microchannel 32 3.4 Numerical simulations 37 3.5 Experiment Preparation 39 3.5.1 Particles Preparation 39 3.5.2 Cell Preparation 40 3.5.3 The Experiment Setup 43 Chapter 4 Results and Discussion 44 4.1 Flow and Pressure Filed Simulation 44 4.2 Particles Separation 50 4.2.1 Particle movements passing through filter 50 4.2.2 Separation of microparticles 53 4.3 Separation of PC3 cells Spiked in Blood Samples 59 4.4 WBCs Separation from Whole Blood 63 4.5 Separation fNRBCs from cord blood 65 Chapter 5 Conclusions 70 5.1 Conclusions 70 5.2 Future Prospects 71 Appendix I 73 Reference 76 | |
dc.language.iso | en | |
dc.title | 藉流體集中增強橫向過濾微流道細胞分離效率之研究 | zh_TW |
dc.title | Enhancement of Microfluidic Cell Separation Using Cross-Flow Filters with Hydrodynamic Focusing | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃振康(Chen-Kang Huang),陳林祈(Lin-Chi Chen),楊馥菱(Fu-Ling Yang) | |
dc.subject.keyword | 微流道,非侵入式檢測,有核紅血球,細胞分離,cross flow, | zh_TW |
dc.subject.keyword | fetal cells,prenatal diagnosis,microfluidics,cross-flow,cells separation, | en |
dc.relation.page | 80 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2015-08-17 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
顯示於系所單位: | 生物機電工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-104-1.pdf 目前未授權公開取用 | 2.99 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。