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標題: | 溫敏性奈米凝膠結合光熱分子應用於藥物制放 Thermal-responsive nanogels containing photothermal molecules for controlled drug release |
作者: | Ray Chang 張睿 |
指導教授: | 蔡偉博(Wei-Bor Tsai) |
關鍵字: | 奈米凝膠,溫敏性,N-異丙基丙烯醯胺,銅葉綠素鈉,N-羥甲基丙烯醯胺,藥物輸送, nanogels,thermo-sensitive,N-Isopropylacrylamide,Sodium copper chlorophyllin,N-(Hydroxymethyl)acrylamide,drug delivery, |
出版年 : | 2015 |
學位: | 碩士 |
摘要: | 近年來,已有多種奈米凝膠的藥物釋放策略(例如:酸鹼、磁或熱敏感等)被發展出來。在眾多釋放策略中,由於溫度的改變相對於其他環境刺激容易調控,故目前較多研究著墨於溫敏性奈米凝膠的開發。然而,多數研究其奈米凝膠控制釋放藥物的溫度多在37 oC左右,這可能會使得膠體在進入生物體後,便開始釋放藥物。本研究藉由N-異丙基丙烯醯胺(N-Isopropylacrylamide, NIPAAm),N-羥甲基丙烯醯胺(N-(Hydroxymethyl)acrylamide, NHMAAm)共聚,將奈米凝膠控制釋放藥物的溫度提升至40 oC以上。此外,我們會在此系統中加入少量的銅葉綠素鈉(Sodium copper chlorophyllin, SCC),使奈米凝膠具有光熱轉換能力。 實驗結果發現,當N-異丙基丙烯醯胺、N-羥甲基丙烯醯胺以及銅葉綠素鈉的比例為80:19:1時,奈米凝膠會有較佳的光熱轉換能力及溫敏性質。而此奈米凝膠可包覆抗癌藥物,並可透過照射雷射調控藥物釋放。此外,細胞存活率的結果顯示,藉由雷射來調控藥物釋放可明顯提升其毒殺細胞的能力。 Recently, many drug release strategies, such as pH-, magnetic-, or thermo- responsive, have been developed to design stimulus responsive nanogels. Among all strategies, temperature stimuli can be easily designed and controlled relative to other environmental stimuli. Thus, much attention has been focused on thermo-sensitive nanogels. However, many researches show that temperature for triggered drug release from nanogels is nearly to 37 oC, which will lead to a rapid release of drug after injection of drug loaded nanogels into human body. In this study, the nanogels which can release drug at higher temperature (above 40 oC) were preparaed by via copolymerization of N-Isopropylacrylamide (NIPAAm) and N-(Hydroxymethyl)acrylamide (NHMAAm). In addition, the small amount of Sodium copper chlorophyllin (SCC) was incorporated into the nanogels, which make nanogels can convert laser into heat. As result, when the ratio of NIPAAm to NHMAAm to SCC was 80: 19: 1, nanogels would have high photothermal efficiency and appropriate thermoresponsive properties. These nanogels could load the anticancer drug and controlled drug release upon exposure to green laser. In addition, the result of cell viability shows that laser-triggered drug release could enhance the cytotoxicity, obviously. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19918 |
全文授權: | 未授權 |
顯示於系所單位: | 化學工程學系 |
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