請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19856完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 潘建源(Chien-Yuan Pan) | |
| dc.contributor.author | Yi-Ting Wang | en |
| dc.contributor.author | 王怡婷 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:23:22Z | - |
| dc.date.copyright | 2015-09-25 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-19 | |
| dc.identifier.citation | 6. References
Ames, J.B., and Lim, S. (2012). Molecular Structure and Target Recognition of Neuronal Ca2+ Sensor Proteins. Biochimica et Biophysica Acta 1820, 1205-1213. Barouch, W., Prasad, K., Greene, L., and Eisenberg, E. (1997). Auxilin-Induced Interaction of the Molecular Chaperone Hsc70 with Clathrin Baskets. Biochemistry 36, 4303-4308. de Barry, J., Janoshazi, A., Dupont, J.L., Procksch, O., Chasserot-Golaz, S., Jeromin, A., and Vitale, N. (2006). Functional Implication of Neuronal Calcium Sensor-1 and Phosphoinositol 4-Kinase-Beta Interaction in Regulated Exocytosis of Pc12 Cells. J Biol Chem 281, 18098-18111. Denessiouk, K., Permyakov, S., Denesyuk, A., Permyakov, E., and Johnson, M.S. (2014). Two Structural Motifs within Canonical Ef-Hand Calcium-Binding Domains Identify Five Different Classes of Calcium Buffers and Sensors. PLoS ONE 9, e109287. Edvardson, S., Cinnamon, Y., Ta-Shma, A., Shaag, A., Yim, Y.-I., Zenvirt, S., Jalas, C., Lesage, S., Brice, A., Taraboulos, A., et al. (2012). A Deleterious Mutation in Dnajc6 Encoding the Neuronal-Specific Clathrin-Uncoating Co-Chaperone Auxilin, Is Associated with Juvenile Parkinsonism. PLoS ONE 7, e36458. Eisenberg, E., and Greene, L.E. (2007). Multiple Roles of Auxilin and Hsc70 in Clathrin-Mediated Endocytosis. Traffic 8, 640-646. Gruschus, J.M., Greene, L.E., Eisenberg, E., and Ferretti, J.A. (2004). Experimentally Biased Model Structure of the Hsc70/Auxilin Complex: Substrate Transfer and Interdomain Structural Change. Protein Science : A Publication of the Protein Society 13, 2029-2044. Handley, M.T., Lian, L.Y., Haynes, L.P., and Burgoyne, R.D. (2010). Structural and Functional Deficits in a Neuronal Calcium Sensor-1 Mutant Identified in a Case of Autistic Spectrum Disorder. PLoS One 5, e10534. Hormuzdi, S.G., Filippov, M.A., Mitropoulou, G., Monyer, H., and Bruzzone, R. (2004). Electrical Synapses: A Dynamic Signaling System That Shapes the Activity of Neuronal Networks. Biochimica et biophysica acta 1662, 113-137. Hui, H., McHugh, D., Hannan, M., Zeng, F., Xu, S.Z., Khan, S.U., Levenson, R., Beech, D.J., and Weiss, J.L. (2006). Ca2+-Sensing Mechanism in Trpc5 Channels Contributing to Retardation of Neurite Outgrowth. J Physiol 572, 165-172. Kabbani, N., Negyessy, L., Lin, R., Goldman-Rakic, P., and Levenson, R. (2002). Interaction with Neuronal Calcium Sensor Ncs-1 Mediates Desensitization of the D2 Dopamine Receptor. J Neurosci 22, 8476-8486. Leitz, J., and Kavalali, E.T. (2015). Ca2+ Dependence of Synaptic Vesicle Endocytosis. Neuroscientist. McFerran, B.W., Graham, M.E., and Burgoyne, R.D. (1998). Neuronal Ca2+ Sensor 1, the Mammalian Homologue of Frequenin, Is Expressed in Chromaffin and Pc12 Cells and Regulates Neurosecretion from Dense-Core Granules. J Biol Chem 273, 22768-22772. Morgan, J.R., Prasad, K., Jin, S., Augustine, G.J., and Lafer, E.M. (2001). Uncoating of Clathrin-Coated Vesicles in Presynaptic Terminals: Roles for Hsc70 and Auxilin. Neuron 32, 289-300. Newmyer, S.L., Christensen, A., and Sever, S. (2003). Auxilin-Dynamin Interactions Link the Uncoating Atpase Chaperone Machinery with Vesicle Formation. Dev Cell 4, 929-940. Pan, C.Y., Jeromin, A., Lundstrom, K., Yoo, S.H., Roder, J., and Fox, A.P. (2002). Alterations in Exocytosis Induced by Neuronal Cacium Sensor-1 in Bovine Chromaffin Cells. J Neurosci 22, 2427-2433. Pongs, O., Lindemeier, J., Zhu, X.R., Theil, T., Engelkamp, D., Krah-Jentgens, I., Lambrecht, H., Koch, K.W., Schwemer, J., Rivosecchi, R., et al. (1993). Frequenin—a Novel Calcium-Binding Protein That Modulates Synaptic Efficacy in the Drosophila Nervous System. Neuron 11, 15-28. Sever, S., Skoch, J., Newmyer, S., Ramachandran, R., Ko, D., McKee, M., Bouley, R., Ausiello, D., Hyman, B.T., and Bacskai, B.J. (2006). Physical and Functional Connection between Auxilin and Dynamin During Endocytosis. Embo j 25, 4163-4174. Sudhof, T.C. (2004). The Synaptic Vesicle Cycle. Annu Rev Neurosci 27, 509-547. Taverna, E., Francolini, M., Jeromin, A., Hilfiker, S., Roder, J., and Rosa, P. (2002). Neuronal Calcium Sensor 1 and Phosphatidylinositol 4-Oh Kinase Beta Interact in Neuronal Cells and Are Translocated to Membranes During Nucleotide-Evoked Exocytosis. J Cell Sci 115, 3909-3922. Wang, X., Zhao, K., Kirberger, M., Wong, H., Chen, G., and Yang, J.J. (2010). Analysis and Prediction of Calcium-Binding Pockets from Apo-Protein Structures Exhibiting Calcium-Induced Localized Conformational Changes. Protein Science 19, 1180-1190. Weiss, J.L., Archer, D.A., and Burgoyne, R.D. (2000). Neuronal Ca2+ Sensor-1/Frequenin Functions in an Autocrine Pathway Regulating Ca2+ Channels in Bovine Adrenal Chromaffin Cells. J Biol Chem 275, 40082-40087. Weiss, J.L., and Burgoyne, R.D. (2001). Voltage-Independent Inhibition of P/Q-Type Ca2+ Channels in Adrenal Chromaffin Cells Via a Neuronal Ca2+ Sensor-1-Dependent Pathway Involves Src Family Tyrosine Kinase. J Biol Chem 276, 44804-44811. Weiss, J.L., Hui, H., and Burgoyne, R.D. (2010). Neuronal Calcium Sensor-1 Regulation of Calcium Channels, Secretion, and Neuronal Outgrowth. Cell Mol Neurobiol 30, 1283-1292. Xing, Y., Bocking, T., Wolf, M., Grigorieff, N., Kirchhausen, T., and Harrison, S.C. (2010). Structure of Clathrin Coat with Bound Hsc70 and Auxilin: Mechanism of Hsc70-Facilitated Disassembly. Embo j 29, 655-665. Yanez, M., Gil-Longo, J., and Campos-Toimil, M. (2012). Ca2+ Binding Proteins. Adv Exp Med Biol 740, 461-482. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19856 | - |
| dc.description.abstract | 在突觸前神經末梢，鈣離子透過結合蛋白啟動胞吐作用釋放神經傳導物質。Neuronal calcium sensor-1 (NCS-1) 含有EF-motif 結合鈣離子和N端荳蔻酸化修飾以嵌入細胞膜，屬於鈣離子結合蛋白的一員。已知NCS-1會調節鈣離子通道或結合胞吞作用相關蛋白，進而調控突觸前囊泡循環，但相關細節尚不清楚。Auxilin為一伴護蛋白，參與在胞吞作用中幫助網格蛋白脫離內吞囊泡表面。本實驗室先前以酵母菌雙雜合系統中證實NCS-1和Auxilin有相互作用，因此在本研究中想了解NCS-1和Auxilin在牛腎上腺嗜鉻細胞中，如何影響囊泡循環。透過安培紀錄法，以高鉀重複刺激後發現，NCS-1延遲胞吐作用發生，推論NCS-1 會減少可立即釋放的囊泡。NCS-1G2A點突變會延長囊泡膜與突觸膜之融合過程。另外，發現NCS-1E120Q點突變可能會降低神經傳導物質填入囊泡之效果。而利用螢光共振轉移和共軛焦顯微鏡影像技術，在NCS-1-EYFP和ECFP-Auxilin C half 共同表現的PC12細胞上，給予ATP刺激時會使EYFP螢光強度暫時下降。推測NCS-1和Auxilin受到ATP刺激會短暫分離後再次結合。本研究主題釐清了NCS-1和Auxilin在囊泡循環中扮演之功能，深入突觸傳遞之分子機制進行探討。 | zh_TW |
| dc.description.abstract | At the presynaptic terminals, calcium triggers the exocytosis mediated by various calcium binding proteins. Neuronal calcium sensor-1 (NCS-1) belongs to EF-hand superfamily of calcium binding proteins and has an N-terminal myristoylation for membrane association. NCS-1 does not only regulate exocytosis through Ca2+ channels but interact with endocyto-sis related protein. However, the detail how NCS-1 affect the vesicle recycling is not clear. Auxilin is a chaperone molecule involved in endocytosis, facilitating uncoating of clathrin and AP2 from endocytosed vesicle. We have previously verified that NCS-1 interacts with auxilin by yeast-two-hybridization. Therefore, we are interested in how NCS-1 and auxilin regulate the exo- and endocytosis using amperometry recording and calcium imaging. Re-petitive high K+ evoked exocytosis was investigated using bovine chromaffin cells express-ing NCS-1 and mutants. Amperometry recording shows that NCS-1 delayed the spike dis-tribution, suggesting that NCS-1 might decrease the size of the readily releasable pool. The prolonged halfwidth infers that NCS-1G2A might increase the fusion period. In addition, NCS-1E120Q diminished the quanta size, indicating NCS-1 might be involved in neurotrans-mitter filling. When NCS-1-EYFP and ECFP-auxilin C-half were coexpressed in PC12 cells, both Fluorescence resonance energy transfer (FRET) and confocal images revealed that YFP intensity dropped upon ATP stimulation, suggesting that NCS-1 and auxilin dissociate dur-ing stimulation. These results demonstrate NCS-1 regulate the kinetics of fusion pore during exocytosis and may interact with auxilin to regulate the vesicle recycling. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:23:22Z (GMT). No. of bitstreams: 1 ntu-104-R02454014-1.pdf: 2112635 bytes, checksum: e7d1b563e12d3c9568b5a27efb2f47b1 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | Catalog
誌謝 i Abstract ii 摘要 iv 1. Introduction 1 1.1. Calcium binding protein (CaBP) 1 1.2. Vesicle recycling 2 1.3. Neuronal Calcium Protein-1 (NCS-1) 4 1.4. Auxilin-1 5 1.5. Aim 7 2. Material and methods 8 2.1. Chemicals 8 2.2. Plasmid transformation and preparation 9 2.3. Isolation and primary culture of bovine chromaffin cells 9 2.4. Bovine chromaffin cell transfection 10 2.5. Amperometry Recording 11 2.6. Ca2+ Imaging 12 2.7. Fluorescence Resonance Energy Transfer (FRET) imaging 12 2.8. Data Analysis 13 3. Results 14 3.1. NCS-1 decreases the Ca2+ response 14 3.2. Cells overexpressing NCS-1 have a delayed release 14 3.3. NCS-1G2A and NCS-1E120Q decrease the release level 16 3.4. NCS-1 mutants affect the the rise and decay time 17 3.5. NCS-1G2A and NCS-1E120Q decrease the PSF area and duration 17 3.6. Emission of CFP and YFP have converse response in FRET imaging 18 3.7. NCS-1 and auxilin translocated after ATP stimulation 19 4. Discussion 20 4.1. NCS-1 participate in intracellular Ca2+ level 20 4.2. NCS-1 detain the vesicle releasing 21 4.3. NCS-1 maitain the normal quanta size and releasing machinery 22 4.4. NCS-1 might have interaction with auxilin 23 5. Conclusion 24 6. References 25 7. Schemes and Figures 29 | |
| dc.language.iso | en | |
| dc.title | NCS-1和Auxilin對牛腎上腺嗜鉻細胞胞吐作用的影響 | zh_TW |
| dc.title | Effects of NCS-1 and Auxilin on Exocytosis in Bovine Chromaffin Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃憲松(Hsien-Sung Huang),王致恬(Chih-Tien Wang) | |
| dc.subject.keyword | NCS-1,auxilin,囊泡循環,牛腎上腺嗜鉻細胞,安培紀錄法, | zh_TW |
| dc.subject.keyword | NCS-1,auxilin,vesicle recycle,chromaffin cell,amperometry, | en |
| dc.relation.page | 42 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2015-08-19 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 腦與心智科學研究所 | zh_TW |
| 顯示於系所單位： | 腦與心智科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-104-1.pdf 未授權公開取用 | 2.06 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。