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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
| dc.contributor.author | Hsin-Kuei Liu | en |
| dc.contributor.author | 劉炘魁 | zh_TW |
| dc.date.accessioned | 2021-06-08T02:05:49Z | - |
| dc.date.copyright | 2020-08-24 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-17 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19565 | - |
| dc.description.abstract | 快速靈敏的臨床檢測能夠增進疾病診斷及疾病擴散的控制，但目前臨床上針對基因檢測所使用的即時聚合酶連鎖反應，因前處理過程複雜且檢測時間過長，並不適合運用於快速臨床檢測。因此，本研究旨在開發一個新型的檢測系統，藉由在奈米金膜上針對標的基因進行環形恆溫擴增法過程反應產物造成的溶液折射率改變，配合波長850nm光與影像感光耦合元件的表面電漿子共振感測系統進行即時訊號擷取，依據所產生的角度與強度的量測對應折射率變化所產生的光學訊號改變。進一步搭配核酸熔解曲線進行分析，讓不同長短的雙股核酸片段在不同溫度下解離，進一步的共振角度位移。此新式的檢測系統有以下優點，首先，因為表面電漿子共振具有高敏感度與即時的特點，可以實現低濃度快速檢測與分子交互作用的動態分析。接著，因為環形恆溫擴增法上針對引子的設計，可以維持檢測的高靈敏度及高特異性。最後，再藉由核酸熔解曲線來達到多標的分析確認。經由前述幾項優點，以改進先前所述臨床上之不足並實現早期臨床檢測的目的。 | zh_TW |
| dc.description.abstract | Rapid and high sensitivity clinical diagnostic tools can improve disease detection and disease-spread control. Real-time PCR is recently used in clinical genetic detection , but its complex sample pretreatment and long detection time make it unsuitable for rapid clinical diagnosis. Thus , we propose a new diagnostic system based on surface plasmon resonance (SPR) angle shift , which is caused by performing loop-mediated isothermal amplification(LAMP) on target sequence on gold film. Furthermore , we perform melting curve analysis , which can cause different refractive index change in different target , to differentiate multiple target sequence. By using this new diagnostic system , we can achieve high sensitivity , high specificity , rapid and real-time multiplex detection. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T02:05:49Z (GMT). No. of bitstreams: 1 U0001-1608202013384800.pdf: 4943521 bytes, checksum: 3bb102fddc87dc10c69fbfde80f937fb (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 目錄 摘要 I ABSTRACT II 圖目錄 VI 表目錄 VIII 第一章 前言 1 1.1研究背景 1 1.1.1傳染病的爆發 1 1.1.2基因分析 3 1.1.3生物感測器之應用 4 1.2研究動機 5 1.3論文架構 6 第二章 文獻回顧與探討 7 2.1抗甲氧西林金黃色葡萄球菌（MRSA） 7 2.1.1 MRSA背景 7 2.1.2致病及抗藥機制 8 2.1.3檢測方式 9 2.2檢測技術 11 2.2.1基因檢測技術的發展 11 2.2.2多基因分析 13 2.2.3 LAMP反應及檢測 14 2.3生物感測器 16 2.3.1 SPR生物感測器的發展 16 2.3.2表面電漿子感測器反應機制 17 2.3.3表面電漿子感測器的優缺點 19 第三章 系統架構與實驗方法 21 3.1感測器軟硬體架構 21 3.1.1光學系統 21 3.1.2晶片設計 23 3.1.3加熱系統 25 3.1.4軟體系統 28 3.2 LAMP反應 29 3.2.1標的基因 29 3.2.2引子設計 29 3.2.3 SPR-LAMP反應條件 31 3.2.4 REAL-TIME LAMP反應條件 32 第四章 實驗結果 33 4.1 LAMP反應折射率變化 33 4.2加熱系統優化 34 4.4角度調變之溫度折射率變化 36 4.5 REAL-TIME LAMP分析 39 4.6 SPR-LAMP反應及核酸熔解曲線分析 41 第五章 結論與未來展望 44 第六章 參考資料 46 | |
| dc.language.iso | zh-TW | |
| dc.title | 多標的環形恆溫擴增法應用於表面電漿子感測器 | zh_TW |
| dc.title | Multiplex Loop Mediated Isothermal Amplification on Surface Plasmon Resonance Biosensor | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林致廷(Chih-Ting Lin),黃念祖(Nien-Tsu Huang) | |
| dc.subject.keyword | 表面電漿子共振,環形恆溫擴增法,核酸熔解曲線,生物感測器,共振角度位移, | zh_TW |
| dc.subject.keyword | Surface Plasmon Resonance,Angle-shift,Biosensor,Loop-mediated Isothermal Amplification,Methicillin-resistance Staphylococcus aureus, | en |
| dc.relation.page | 53 | |
| dc.identifier.doi | 10.6342/NTU202003576 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-08-17 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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