Skip navigation

DSpace

機構典藏 DSpace 系統致力於保存各式數位資料(如:文字、圖片、PDF)並使其易於取用。

點此認識 DSpace
DSpace logo
English
中文
  • 瀏覽論文
    • 校院系所
    • 出版年
    • 作者
    • 標題
    • 關鍵字
  • 搜尋 TDR
  • 授權 Q&A
    • 我的頁面
    • 接受 E-mail 通知
    • 編輯個人資料
  1. NTU Theses and Dissertations Repository
  2. 醫學院
  3. 法醫學科所
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19407
標題: 利用尿液中非法海洛因之雜質代謝物確認非法海洛因之使用
Using Specific Metabolites Originated from Illicit Heroin Impurities to Confirm the Administration of Illicit Heroin in Urine
作者: Po-Hsin Kong
龔柏勳
指導教授: 陳珮珊(Pai-Shan Chen)
關鍵字: 液相層析串聯質譜分析,高解析質譜分析,法醫毒理學,非法海洛因,代謝物鑑定,乙醯化蒂巴因,罌粟植物鹼,體外代謝反應,
Liquid chromatography-tandem mass spectrometry,high resolution mass spectrometry,forensic toxicology,illicit heroin,metabolite identification,acetylated thebaines,alkaloids of Papaver somniferum,in vitro metabolism,
出版年 : 2016
學位: 碩士
摘要: 確認海洛因施用者主要倚靠6-乙醯嗎啡的檢出,僅檢出嗎啡成分不能證明施用海洛因。但由於6-乙醯嗎啡體內半衰期短,限制了6-乙醯嗎啡的應用價值。然而,不同於高純度的全合成海洛因,非法海洛因源產於罌粟中之嗎啡,成品含多種具特異性的乙醯化植物鹼雜質。為輔助確認施用海洛因,我們利用人類肝細胞成分模擬人體代謝反應,於體外合成此類特異性雜質的可能代謝物,依此開發液相層析質譜檢測方法,並分析已知為海洛因使用者的尿液檢體。本研究經由分析精確質量與碎片圖譜找到12個代謝物,其中8個未曾報導過。此外,代謝物ATM4S在真實檢體中驗出,14件6-乙醯嗎啡陽性檢體中檢出9件,7件嗎啡陽性而6-乙醯嗎啡陰性的檢體中檢出2件。暗示ATM4S應與6-乙醯嗎啡有相關性,且體內陽性反應時間可能超過6-乙醯嗎啡。
關鍵字:液相層析串聯質譜分析、高解析質譜分析、法醫毒理學、非法海洛因、代謝物鑑定、乙醯化蒂巴因、罌粟植物鹼、體外代謝反應
In the forensic toxicology, it is a challenge to confirm heroin administration, especially for those who are only be confirmed the presence of morphine but no detectable 6-acetylmorphine in their biological samples. However, illicit heroin, different from pharmaceutical heroin with high purity, is manufactured from the capsules of Papaver somniferum with a plenty of alkaloid impurities. Thebaine, one of the alkaloids, can be acetylated during the manufacturing process, and forming unusual rearrangement products, which have been thought to be specific features of illicit heroin. To apply these impurities as a marker in biological fluid to confirm heroin administration, we in vitro synthesized the phase I and phase II metabolites with human liver subfractions, and established a method using liquid chromatography-mass spectrometry. Urine samples from heroin abuser were analyzed to verify if the metabolites from acetylated thebaines can be detected. There were 12 metabolites identified by accurate masses and fragmentation patterns, and 8 of which have never been reported before. Besides, metabolite – ATM4S was detected in the real urine samples: 9 out of 14 samples presented with 6-acetylmorphine; 2 out of 7 samples only presented with morphine.
Keywords: Liquid chromatography-tandem mass spectrometry; high resolution mass spectrometry; forensic toxicology; illicit heroin; metabolite identification; acetylated thebaines; alkaloids of Papaver somniferum; in vitro metabolism
URI: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19407
DOI: 10.6342/NTU201600487
全文授權: 未授權
顯示於系所單位:法醫學科所

文件中的檔案:
檔案 大小格式 
ntu-105-1.pdf
  目前未授權公開取用
3.29 MBAdobe PDF
顯示文件完整紀錄


系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。

社群連結
聯絡資訊
10617臺北市大安區羅斯福路四段1號
No.1 Sec.4, Roosevelt Rd., Taipei, Taiwan, R.O.C. 106
Tel: (02)33662353
Email: ntuetds@ntu.edu.tw
意見箱
相關連結
館藏目錄
國內圖書館整合查詢 MetaCat
臺大學術典藏 NTU Scholars
臺大圖書館數位典藏館
本站聲明
© NTU Library All Rights Reserved