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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 曾麗慧(Li-Hui Teng) | |
dc.contributor.author | Kai-Tzu Kao | en |
dc.contributor.author | 高愷孜 | zh_TW |
dc.date.accessioned | 2021-06-08T01:45:36Z | - |
dc.date.copyright | 2016-08-26 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-12 | |
dc.identifier.citation | 1. Key statistics for colorectal cancer. 2016. (Accessed 2016/07/18, 2016, at
http://www.cancer.org/cancer/colonandrectumcancer/detailedguide/colorectal-cancer-ke y-statistics.) 2. Nussbaum RL, McInnes RR, Willard HF. Thompson & Thompson genetics in medicine. Eighth edition. ed. 3. Dudley JC, Lin MT, Le DT, Eshleman JR. Microsatellite Instability as a Biomarker for PD-1 Blockade. Clin Cancer Res 2016;22:813-20. 4. Hampel H, Frankel W, Panescu J, et al. Screening for Lynch syndrome (hereditary nonpolyposis colorectal cancer) among endometrial cancer patients. Cancer Res 2006;66:7810-7. 5. Zighelboim I, Goodfellow PJ, Gao F, et al. Microsatellite instability and epigenetic inactivation of MLH1 and outcome of patients with endometrial carcinomas of the endometrioid type. J Clin Oncol 2007;25:2042-8. 6. Tseng LH, Tang JL, Haley L, et al. Microsatellite instability confounds engraftment analysis of hematopoietic stem-cell transplantation. Appl Immunohistochem Mol Morphol 2014;22:416-20. 7. Merok MA, Ahlquist T, Royrvik EC, et al. Microsatellite instability has a positive prognostic impact on stage II colorectal cancer after complete resection: results from a large, consecutive Norwegian series. Ann Oncol 2013;24:1274-82. 8. Funkhouser WK, Jr., Lubin IM, Monzon FA, et al. Relevance, pathogenesis, and testing algorithm for mismatch repair-defective colorectal carcinomas: a report of the association for molecular pathology. J Mol Diagn 2012;14:91-103. 9. Berg KD, Glaser CL, Thompson RE, Hamilton SR, Griffin CA, Eshleman JR. Detection of microsatellite instability by fluorescence multiplex polymerase chain reaction. J Mol Diagn 2000;2:20-8. 10. Hatch SB, Lightfoot HM, Jr., Garwacki CP, et al. Microsatellite instability testing in colorectal carcinoma: choice of markers affects sensitivity of detection of mismatch repair-deficient tumors. Clin Cancer Res 2005;11:2180-7. 11. Gryfe R, Kim H, Hsieh ET, et al. Tumor microsatellite instability and clinical outcome in young patients with colorectal cancer. N Engl J Med 2000;342:69-77. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/19117 | - |
dc.description.abstract | 本論文的目的利用微小衛星不穩定性(Microstatellite instability)與遺傳性非瘜肉症結直 腸癌(Hereditary non-polyposis colorectal cancer, HNPCC)的高度相關的特性,來分析台灣地 區微小衛星不穩定性在大腸直腸癌的表現,以作為臨床上評估大腸直腸癌患者預後的情況。
隨著台灣地區飲食習慣西化的影響,導致台灣地區大腸直腸癌發生率有逐年增加的情 況。除了飲食習慣等環境因素影響外,與遺傳基因有關的大腸直腸癌大約佔 15%。 本研究共收集了經臺大醫院確診的大腸直腸癌患者 265 位,分別為 89 位第二期患者與 176 位第四期患者,利用 Fluorescence Multiplex Polymerase Chain Reaction 與毛細管電泳分 析,來偵測台灣地區微衛星不穩定性在大腸直腸癌的表現。 結果顯示大腸直腸癌第四期患者較第二期患者在年紀、性別、MSS 的表現均有顯著差異, 反觀在第二期與第四期的 MSI-H 表現則沒有顯著差異性。而進一步比較第二期與第四期大腸 直腸癌患者在性別上的表現是無差異性的,且在年紀上也無差異性。本實驗結果與美國 Hatch SB, Lightfoot HM 等人在 2010 年北卡羅來納州所實驗的結果相近。 | zh_TW |
dc.description.abstract | The objective of this study is to utilize the high relevance of the characteristics of Microsatellite instability and Hereditary non-polyposis colorectal cancer (HNPCC) and to analyze the performance of Microsatellite instability and colorectal cancer in Taiwan and in the assessment of prognosis of colorectal cancer patients.
With the westernization of the eating habits in Taiwan, incidence of colorectal cancer in Taiwan increases through the years, Aside from eating habits and environmental factors, heredity may also stands approximately 15% of possible causes. This study collected data from 265 subjects diagnosed with colorectal cancer in the National Taiwan University Hospital, of which 89 subjects are in the 2nd stage and 176 subjects in the 4th stage, using the Fluorescence Multiplex Polymerase Chain Reaction and capillary electrophoresis method to detect the performance of microsatellite instability in colorectal cancer in Taiwan. Results show significant difference observed in subjects with 4th stage and 2nd stage subjects in their ages, gender and MSS performance, but in contrast, there is so significant difference seen in MSI-H performance. To further compare subjects in the 2nd and 4th stages, there is no significant difference neither in gender nor in age The observed outcome is very similar to that made by USA Hatch SB, Lightfoot HM et al. in 2010 in North Carolina. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T01:45:36Z (GMT). No. of bitstreams: 1 ntu-105-P03448012-1.pdf: 2071273 bytes, checksum: dc7f39b2eccbca5beac6fd41ff92af2f (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 口試委員審定書 i
致謝 ii 中文摘要 iii Abstract iv 目錄 v 表目錄 vii 圖目錄 viii 第一章 前言 1 第二章 文獻考證 3 第一節 大腸直腸癌(Colorectal cancer, CRC)的介紹 3 第二節 與遺傳有關的大腸直腸癌 4 第一項 家族腺瘤性瘜肉症(Familial adenomatous polyposis, FAP) 4 第二項 遺傳性非瘜肉症結直腸癌(Hereditary non-polyposis colorectal cancer, HNPCC) 5 第三節 微衛星不穩定現象Microstatellite Instability, MSI 6 第四節 研究目的 9 第三章 研究方法 10 第一節 研究對象與來源 10 第一項 研究對象 10 第二項 方法原理 10 第三項 實驗步驟 11 第四章 結果 14 第一節 描述性統計分析之結果: 14 第一項 以年紀觀察: 15 第二項 以性別來觀察: 16 第三項 MSI在CRC的表現: 16 第五章 結論 18 第一節 Norwegian結果 18 第一項 MSI在CRC的表現 18 第二項 以性別來觀察 18 第二節 USA結果 19 第一項 MSI在CRC的表現 19 第二項 以性別來觀察 19 第三節 Canada結果 19 第四節 NTU與USA、Norwegian、Canada比較 20 第一項 MSI-H在CRC的表現 20 第二項 以性別來比較 20 第三項 以年紀來比較 21 第五節 總結 21 第六章 遺傳諮詢與醫學倫理 22 第七章 參考文獻 23 第八章 圖表 24 | |
dc.language.iso | zh-TW | |
dc.title | "台灣地區大腸直腸癌微衛星不穩定性(Microsatellite instability, MSI)的表現" | zh_TW |
dc.title | Microstatellite Instability Manifestation of Colorectal Cancer in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 李建南(Chine-Nan Lee) | |
dc.contributor.oralexamcommittee | 林芯? | |
dc.subject.keyword | 微小衛星不穩定性,遺傳性非瘜肉大腸直腸癌,大腸直腸癌, | zh_TW |
dc.subject.keyword | Microsatellite instability,Hereditary non-polyposis colorectal cancer,colorectal cancer, | en |
dc.relation.page | 43 | |
dc.identifier.doi | 10.6342/NTU201602251 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2016-08-12 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
顯示於系所單位: | 分子醫學研究所 |
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