牙髓幹細胞於氯化鋅/聚己内酯電紡紗薄膜之生長及分化

Abstract

齲齒是國人口腔衛生健康最常見的病灶之一。如果放任輕微齲齒不處理，往往會造成齲齒範圍擴大，影響到神經活性，最後導致牙齒需要做根管治療的命運。一但牙齒經過根管治療，對於牙齒的健康也已大打折扣。因此近年來，牙科治療已朝向盡量保留牙髓活性的方向進展。覆髓材料主要為了在齲齒面積較大時能盡量覆蓋於牙髓上方保留牙髓活性而不至於在補牙過程中導致牙髓細胞壞死。 電紡紗(electrospinning)是近年來組織工程中新興的材料製備方法之一。藉由電紡紗製備材料可將有機高分子材料製做成奈米等級大小的紡絲。電紡紗材料具有高表面積，可調整粗細，可攜帶藥物，可調控通透性等特色，因此做為細胞生長框架，也適合作為攜帶藥物之基材。 本研究以聚己內酯(polycaprolactone)為基材，混和氯化鋅(ZnCl2)製作出ZnCl2/PCL為覆髓材料的評估。先以生物相容性測試(MTT assay)確認這樣的材料能擁有良好的生物相容性。在以茜素紅S染色法(ARS assay)以及鹼性磷酸酶活性分析(ALP activity test)檢測發現材料擁有促進牙髓幹細胞分化及產生鈣化沉積。因此，利用電紡紗製備方法製作出ZnCl2/PCL可促進牙髓幹細胞的生長分化。本論文製作出一種新的電紡紗薄膜可應用於牙髓覆髓材料之使用。

Dental caries has been the most common disease in Taiwan's health care. Usually, when a caries cavity was left untreated, the caries will soon spread to dental pulp area, eventually the pulp will be extracted, and the tooth lost its vitality. Recent years, preserving dental pulp has been the most important goal in dental treatment, and pulp capping materials is for that purpose. Electrospinning is a new method to create materials in tissue engineering in recent years. By using this method, we can make nano-sized polymer fiber, and fabricate fiber mesh according to our needs. Electrospun fiber mesh has many advantages, including large surface area, easy to modify physical property, drug delivery via coating or blending, permeability to liquid. For this reason, the electrospun fiber mesh can serve as not only cell growth structure, but also drug carrier. In this study, we fabricate electrospun polycaprolactone fiber mesh, and blended with ZnCl2, and evaluate it as a pulp capping mateiral. Zinc chloride is known to be related to bone metabolism. A recent study shows that zinc can promote dental pulp stem cells(DPSCs) differentiation. We fabricate electrospun nanofiber blended with zinc chloride, and found this material not only have good bio-compatibility, but also good at inducing DPSCs differentiation and calcium deposition, both results support that our new material has 2 good odontogenic induction ability. The present study suggests a new type of material, to improve traditional capping method. I hope this material has potential useful gradually in clinical use in the future.