請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18732
標題: | 阿拉伯芥 AtMAPR3 參與對抗灰黴病菌感染之功能性研究 Functional studies of Arabidopsis AtMAPR3 in the defense against B. cinerea |
作者: | Huan-Yu Lin 林煥宇 |
指導教授: | 楊健志(Chien-Chih Yang) |
關鍵字: | MAPR,灰黴病菌,活性氧化物質,植物防禦系統,水楊酸, Membrane-associated progesterone receptor,Botrytis cinerea,reactive oxygen species,plant defense,salicylic acid, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 膜聯合孕酮受質蛋白 (MAPRs) 是一種廣泛存在於真核生物的血基質結合蛋白質,存在於動物、植物及真菌中。AtMAPR 是 MAPR 家族在阿拉伯芥中的同源蛋白,目前已知的功能有參與去除內質網壓力 (ER stress) 與參與木質素的生合成等等。本研究探討 AtMAPR3 在植物防禦上可能扮演的角色。根據微陣列資料庫的資料顯示,AtMAPR3 的表現量會被植物病原菌灰黴病菌、丁香假單胞菌等病原菌感染所誘導。其中在灰黴病菌感染後, AtMAPR3 表現量提升最高。為了瞭解 AtMAPR3 是否參與對抗灰色葡萄孢菌的防禦,本研究利用兩個 AtMAPR3 功能缺失的突變株 (msbp2-1 和 msbp2-2) 進行研究。 msbp2 突變株中被灰黴病菌感染後,感染面積比野生型植株大 1.2 倍。然而在感染的突變株植株中,病菌的生長情況與野生型植株無明顯相異。此結果暗示 msbp2 突變株的初期防禦機制受損,但是當植物防禦啟動後,突變株能夠限制感染真菌的生長。分析感染後不同時間產生的活性氧化物質 (ROS),發現在感染 48 小時後,msbp2 突變株中的 ROS 累積量比野生型多,這和呼吸爆發氧化酶 D (RBOHD) 的表現量增加相關。此外,突變株中水楊酸 (SA) 反應基因表現量也比較高。綜合上述,缺乏 AtMAPR3 似乎影響病原菌感染的早期感應階段。AtMAPR3 可能在辨識病原菌上或是早期防禦訊息傳導上扮演了某個角色,所以後續引發的防禦才會被延遲。 Membrane-associated progesterone receptors (MAPRs) are widely found in eukaryotes, including animals, plants, and fungi. The functions of MAPR in Arabidopsis (AtMAPR, also named as MSBPs) are diversified, ranging from participating in the release of ER stress to lignin biosynthesis. Here, the possible role of AtMAPR3 in plant defense is studied. Based on microarray databases, the expression of AtMAPR3 can be induced by the infection of phytopathogen Botrytis cinerea (B. cinerea), Pseudomonas syringae (P. syringae), etc.; among these factors, B. cinerea has been shown to generate the highest induction levels of AtMAPR3 expression. To investigate if AtMAPR3 is involved in plant defense against B. cinerea, two mutants defected in AtMAPR3, namely msbp2-1 and msbp2-2, were employed in this study. The lesion areas of msbp2 infected by B. cinerea were larger by 1.2-fold than that of WT. However, the fungal growth of B. cinerea was similar in the mutants and WT. This suggested that the defense mechanisms in the early stage of infection were impaired in msbp2 mutants. However, the mutants were able to limit the growth of the infected fungi after the activation of late plant defense mechanisms. The ROS accumulation triggered by the infection was detected at different time points post-inoculation of the pathogen. It was interesting to note that ROS accumulated more in msbp2 mutants after 48 hours of infection, as revealed by DAB staining. This enhanced accumulation of ROS in msbp2 mutants correlated with the enhanced expression of the respiratory burst oxidase homolog D (RBOHD) in msbp2 mutants. Furthermore, the expression levels of salicylic acid (SA) responsive genes were also higher in mutants. Taken together, the lack of AtMAPR3 appears to affect the early stage responses for the pathogen infection. AtMAPR3 might likely play roles in pathogen sensing or early signaling, which could have resulted in the delayed triggering of the defense system in the msbp2 mutants. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18732 |
DOI: | 10.6342/NTU202002912 |
全文授權: | 未授權 |
顯示於系所單位: | 生化科技學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
U0001-1108202011082400.pdf 目前未授權公開取用 | 4.56 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。