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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林啟萬 | |
| dc.contributor.author | Yin-Lin Lu | en |
| dc.contributor.author | 盧盈霖 | zh_TW |
| dc.date.accessioned | 2021-06-08T01:05:01Z | - |
| dc.date.copyright | 2014-08-25 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-20 | |
| dc.identifier.citation | [1] 行政院衛生署, “101年主要死因分析” (2013)
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18434 | - |
| dc.description.abstract | 糖化血色素 (Glycated Hemoglobin, HbA1c)是糖尿病的診斷與監測的重要標誌。而在血色素與葡萄糖產生糖基化過程所造成的血色素改變成為光譜檢驗糖化血色素濃度的重要指標。在本研究中，我們分析了利用光譜量測技術對糖化血色素濃度檢測的可行性，而光譜檢測技術不僅是一個敏感度高的檢測方式，同時也具有快速檢測、低成本的檢測系統，並配合漫反射式光譜檢測系統以達到非侵入式糖化血色素光譜檢測系統。
在實驗中，為了確立不同濃度的糖化血色素可藉由光譜檢測，因此進行了血色素糖基化實驗觀察過程中血色素光譜的變化情況，利用帶氧血色素、缺氧血色素與高鐵血色素的光譜特徵波峰進行光譜比值，由結果我們確立不同濃度的糖化血色素能以光譜量測系統檢測，且於糖化血色素濃度為1%~12%時擁有98%的量測線性度。此外，我們還進行了漫反射光譜於糖化血色素的非侵入式檢測的可行性實驗，於仿體漫反射光譜之模擬量測結果中，糖化血色素為3%~12%的量測線性度也達97%；於未來將針對臨床量測部位進行該部位組織散射係數與黑色素的等誤差因子訊號干擾模擬以建立量測校正參數，並將此校正參數實際應用臨床量測以分析非侵入式糖化血色素檢測的可行性。 | zh_TW |
| dc.description.abstract | Glycated hemoglobin (HbA1c) is an important maker in diabetes diagnosis and disease status tracking. In this study, we demonstrate the advantage of using absorption spectroscopy technique for glycated hemoglobin (HbA1c) quantitation. By the ratio of selected absorption peaks, the concentration of HbA1c can be easily determined. Our experimental result shows that absorption spectroscopy is not only a sensitive method in HbA1c detection, but also a high potential technique in developing a noninvasive, rapid and cost effective diabetes monitoring device with.
In our experiments, in order to establish the HbA1c concentration can be detected by spectral, we used the feature peaks of oxy-Hb, deoxy-Hb and metHb to calculate the peak ratio. As the results shown, we demonstrated that different HbA1c levels can be detected by spectral measurement system, and the calibration curve had 98% linearity in 1% to 12% HbA1c levels. In addition, the calibration curve had 97% linearity in 3% to 12% HbA1c levels of phantom spectrum by using the diffuse reflectance spectroscopy in noninvasive measurement experiments. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T01:05:01Z (GMT). No. of bitstreams: 1 ntu-103-R01548026-1.pdf: 1491021 bytes, checksum: bb8f4f9392bad89d4fa93f290842f40f (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 口試委員審定書 II
致謝 II 中文摘要 III ABSTRACT IV 目錄 V 圖目錄 VII 表目錄 VIII 第一章：緒論 1 1.1現有糖化血色素檢測方法與比較 3 1.1.1 陽離子交換高壓液相層析法 3 1.1.2 硼酸親和力層析法 3 1.1.3 酵素免疫分析法 4 1.2光學檢測於光譜式糖化血色素之應用 5 1.3現有非侵入式糖化血色素檢測方式 7 第二章：理論介紹 9 2.1 血色素與糖化血色素簡介 9 2.2 UV/VIS 吸收光譜量測原理 13 2.3 漫反射原理 17 2.4 蒙地卡羅演算法 20 第三章：實驗材料與研究方法 22 3.1 血色素糖基化對光譜之影響 22 3.1.1 血色素糖基化樣本準備 22 3.1.2糖化血色素樣本準備 23 3.1.3 UV/VIS光譜量測系統 24 3.2非侵入式漫反射系統於皮膚淺層糖化血色素檢測 25 3.2.1 漫反射光譜量測系統 26 3.2.2 斜角光纖探頭 26 3.3 仿體量測實驗 28 3.4 人體漫反射光譜臨床量測 31 第四章：實驗結果與討論 32 4.1血色素糖基化與糖化血色素光譜量測 32 4.1.1 血色素糖基化光譜量測結果分析 32 4.1.2 糖化血色素光譜量測結果分析 34 4.2 非侵入式光譜檢測之可行性量測 36 4.2.1組織雙層仿體量測結果 37 4.2.2臨床耳垂光譜量測結果 39 第五章：結論與未來展望 41 參考文獻 42 | |
| dc.language.iso | zh-TW | |
| dc.title | 非侵入式糖化血色素光譜檢測於糖尿病應用可行性研究 | zh_TW |
| dc.title | Feasibility Study on Noninvasive Spectrophotometric Measurement of Glycated Hemoglobin for Diabetics | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 宋孔彬 | |
| dc.contributor.oralexamcommittee | 劉子銘,許正治 | |
| dc.subject.keyword | 糖尿病,糖化血色素,HbA1c,光譜,漫反射光譜,非侵入式檢測,光譜特徵分析, | zh_TW |
| dc.subject.keyword | Diabetics,glycated hemoglobin,HbA1c,spectrophotometric,diffuse reflectance,noninvasive measurement,spectrum feature, | en |
| dc.relation.page | 44 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2014-08-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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