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標題: | 以菠菜開花率修正光穩態平衡模式 Modified Photostationary Equilibrium Model in Flowering of Spinach |
作者: | Zong-Han Yang 楊宗翰 |
指導教授: | 方煒(Wei Fang) |
關鍵字: | 植物工廠,光週期,遠紅光,開花率,光敏素,光穩態平衡, Plant factory,Photoperiod,Far-red,Flowering rate,Phytochrome,Photostationary equilibrium (PSE),Photostationary state (PSS), |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 本研究旨在探討光敏素光穩態平衡 (Photostationary Equilibrium) 與抑制長日植物 (以菠菜為例) 開花的相關性,於明期與暗期分別以冷白 (Cool White, CW) 與遠紅光 (Far-Red, FR) LED 為光源,於全人工光型植物工廠內栽培美和菠菜,目標在維持較長明期以確保產量之同時仍能抑制菠菜開花。 實驗分四個主題進行探討:1. 補充遠紅光之時機,2. 補充遠紅光之總量、強度與均勻度,3. 明期之極限,4. 溫度等對菠菜生長與開花的影響。實驗共 21 個處理組,計算各處理組的光敏素光穩態平衡指標 (PhotoStationary State, PSS),並量測開花率,結果發現兩者相關性很低,進而加入暗期時間與溫度的影響來修正 PSS 模式,最終建立考慮全光譜 (T-PSS) 與只考慮紅光與遠紅光波段的日累積光量 (D-PSS) 兩種修正 PSS 模式,建立其與開花率的相關性,R2 值由 PSS 模式的 0.2 進步到 T-PSS 的 0.92 與 D-PSS 的 0.91。 本研究建立的兩種修正 PSS 模式可成功描述明期 (CW) 與暗期補光 (FR) 及暗期的時間長度與溫度對兩型光敏素之間相互轉換的關係,所建立的方法對於探討其他植物的光敏素轉換應具備參考價值。 The purpose of this study is to explore the correlation between the photostationary equilibrium of phytochromes and the inhibition of the flowering of long-day plants by using spinach as an example. Cool white (CW) and far-red (FR) LEDs are used as light source to cultivate spinach ‘Meiho’ in plant factory with artificial light. The goal is to maintain a longer light period to ensure the yield and to inhibit the flowering of spinach at the same time. The experiments are divided into four parts: 1. Timing to apply the far-red light. 2. The total amount, intensity and uniformity of supplementing far-red light. 3. The limit of light period 4. Temperature. The effects of the above mentioned four targeting parameters on the growth and flowering of spinach were compared. Totally, there are 21 treatment groups in the experiments. The phytochrome photostationary state (PSS) of each treatment is calculated, and the flowering rate is measured. The result shows that the correlation between the two is quite low. The influence of duration and the temperature during the dark period were considered. As a result, two modified PSS models were established: the 1st one considers 300~800 nm (T-PSS) and the other considers only the red (600~700 nm) and far-red (700~800 nm) light bands (D-PSS). The R2 value of the correlation of specific index of the three models with flowering rate has increased from 0.2 in PSS to 0.92 in T-PSS and 0.91 in D-PSS model. Two modified PSS models developed in this study can successfully demonstrate the impact of the CW light during the light period, FR light, duration and temperature of the dark period on the conversion between the Pr and Pfr. The method developed should be valuable to further explore the conversion of phytochrome of other plants. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18410 |
DOI: | 10.6342/NTU202003121 |
全文授權: | 未授權 |
顯示於系所單位: | 生物機電工程學系 |
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