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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 金洛仁(Laurent Zimmerli) | |
| dc.contributor.author | Yi-Ying Hsieh | en |
| dc.contributor.author | 謝宜穎 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:59:43Z | - |
| dc.date.copyright | 2015-04-27 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2015-01-15 | |
| dc.identifier.citation | Reference
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18322 | - |
| dc.description.abstract | 植物在辨識到微生物所共有且演化保守的分子結構時，將啟動先天性免疫反應，而該種分子結構稱為病原菌相關分子模式(pathogen-associated molecular patterns, PAMPs)。此防禦反應稱之為模式誘發免疫反應(pattern-triggered immunity, PTI)。而這種免疫反應近年來已被發現是經由表觀遺傳的甲基化以及乙醯化調控。為了解植物對抗Pseudomonas syringae (Pst)甲基化調控的機制，我們利用KRYPTONITE (KYP) 這個基因的突變株來做為材料，其是專一性的甲基化組蛋白三第九個賴氨酸的雙甲基化轉位酵素。接種了Pst後，突變株的抗性較為野生種(Col-0)強。突變株中的氣孔免疫能力、防禦基因FRK1以及WRKY22的表現量也較野生種來得高。當處理了病原菌相關分子模式，突變株中組蛋白三第九個賴氨酸的雙甲基化程度在防禦基因FRK1以及WRKY22的雙甲基化程度也大幅的減低。從我們的研究得知，在植物以及病原菌的交互關係中，組蛋白甲基化為相當重要的調控角色。 | zh_TW |
| dc.description.abstract | Pathogen-associated molecular patterns (PAMPs) such as flagellin (flg22) are microbial molecular signatures recognized by pattern recognition receptors (PRRs). Recognition leads to the activation of PAMP-triggered immunity (PTI). Many defense-responsive mechanisms involved in PTI are believe to be under epigenetic control mediated by histone acetylation, histone methylation and DNA methyation modifications. To increase our understanding of Arabidopsis thaliana defense mechanisms towards Pseudomonas syringae bacteria, we studied KRYPTONITE (KYP), a methyltransferase specific to Histone 3 Lysine 9 dimethylation (H3K9me2). Loss of function mutants of KYP demonstrated boosted resistance against Pseudomonas syringae when compared to wild-type controls (Col-0). Notably, priming of PTI marker genes such as FRK1 and WRKY22 were observed after flg22 treatment in kyp mutants. In addition, we found decreased enrichment of H3K9me2 at promoter and ATG region of FRK1 and WRKY22 in Col-0 plants treated with the PAMP flg22. Our results suggest that histone H3 lysine 9 dimethylation is a critical component in the signaling and defense processes occurring between plants and microbes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:59:43Z (GMT). No. of bitstreams: 1 ntu-103-R01b42020-1.pdf: 2017138 bytes, checksum: d7e5360e72ea2d121d47a0e09b29116f (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | Contents
誌謝 i 摘要: ii Abstract iii Contents v Introduction 1 Basal immunity responses in plant 1 Priming of Pattern-triggered immunity in Arabidopsis 1 Epigenetic regulation in plant 2 Epigenetic changes associated with biotic Stress Conditions 3 Objective 6 Material and Method 7 Biological Materials and Growth Conditions 7 Bacterial inoculations 7 Screening of homozygous mutant plants 8 RNA extraction and gene expression analysis 9 Histone Extraction 10 SDS-PAGE and Immunoblotting 11 Stomatal Assay 12 Measurement of ROS production 12 ChIP assay 13 Results 14 Epigenetic T-DNA insertion mutant lines screening 14 Genotyping of kyp mutants 15 The level of H3K9me2 in kyp mutants is strongly reduced 15 Mutants kyp-1 and kyp-2 are highly resistant to bacterial pathogen Pst DC3000 16 Mutants kyp-1 and kyp-2 demonstrate increased resistance to necrotrophic bacterial pathogen Pcc SCC1 16 Wild-type level of flg22-induced ROS burst in kyp mutants 17 Increased stomatal immunity in kyp mutants 17 Decreased KYP gene expression levels is in Col-0 WT 18 PAMP-responsive gene expression is increased in kyp mutants 18 Shearing of chromatin 19 H3K9me2 levels decreased at FRK1 and WRKY22 loci upon flg22 treatment 20 Flg22-induced histone 3 modifications are important in PTI 20 Shearing of chromatin from Arabidopsis thaliana for ChIP-seq 22 Discussion 23 Histone 3 Lysine 9 di-methylation plays a critical role in PTI and priming 23 The modification of H3 Lysine 9 di-methylation in PTI is transient 24 The distribution of elongating Pol II 27 Conclusion and Future perspectives 29 Figure 31 Figure 1. Phenotype of Pst DC3000 infected Col-0 and epigenetic mutants 32 Figure 2. Genotypic characterization of KYP T-DNA insertion lines 33 Figure 3. H3K9me2 in kyp mutants 34 Figure 4. kyp mutants are highly resistant to Pst DC3000 35 Figure 5. kyp mutants are more resistant to Pcc SCC1 36 Figure 6. No increase of flg22-induced ROS production in kyp mutants 37 Figure 7. kyp mutants demonstrate an enhanced stomatal immunity. 38 Figure 8. KYP expression level after flg22 treatment 39 Figure 10. Efficient shearing of chromatin from Arabidopsis thaliana 42 Figure 11. Time-course of modifications of histone H3 on FRK1 and WRKY22 treated with different concentration of flg22 44 Figure 12. Modifications of histone H3 after flg22 treatment in Col-0. 47 Figure 13. Modifications of histone H3 Lysine 9 after flg22 treatment. 50 Figure 14. Shearing of chromatin from Arabidopsis thalianan for ChIP-seq 51 Appendix 52 Reference 55 | |
| dc.language.iso | zh-TW | |
| dc.title | 表觀遺傳學對於植物病原微生物交互作用之調控探討 | zh_TW |
| dc.title | Functional analysis of histone methyltransferase KYP/SUVH4 in plant immunity | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林讚標(Tsan-Piao Lin),吳克強(Ke-qian Wu) | |
| dc.subject.keyword | 表觀遺傳學,分子結構稱為病原菌相關分子模式,模式誘發免疫反應,植物防禦,組蛋白甲基化, | zh_TW |
| dc.subject.keyword | Epigenetics,Pathogen-associated molecular patterns (PAMPs),AMP-triggered immunity (PTI),histone methylation,methyltransferase,H3 lysine 9 dimethylation, | en |
| dc.relation.page | 62 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2015-01-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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