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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Chien-Yeh Lee | en |
dc.contributor.author | 李健業 | zh_TW |
dc.date.accessioned | 2021-06-08T00:45:58Z | - |
dc.date.copyright | 2015-08-06 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-07-31 | |
dc.identifier.citation | [1] Carroll M. Leevy, Frank Smith, Jacques Longueville, Gustav Paumgartner, and Maceo M. Howard, 'Indocyanine Green Clearance as a Test for Hepatic Function', JAMA., 200, 3, pp. 236-240, 1967.
[2] T. Desmettre, J.M. Devoisselle, and S. Mordon, 'Fluorescence Properties and Metabolic Features of Indocyanine Green (ICG) as Related to Angiography', Surv. Ophthalmol., 45, 1, pp. 15-27, 2000. [3] Jarmo T. Alander, Ilkka Kaartinen, Aki Laakso, Tommi Pぴatilぴa, Thomas Spillmann, Valery V. Tuchin, Maarit Venermo, and Petri Vぴalisuo, 'A Review of Indocyanine Green Fluorescent Imaging in Surgery', Int. J. Biomed. Imaging., 2012. [4] Robert H Webb, 'Confocal optical microscopy', Rep. Prog. Phys., 59, pp. 427-471, 1996. [5] Douglas B. Murphy, Fundamentals of light Microscopy and Electronic Imaging, A John Wiley and Sons, Inc., U.S.A., 2001. [6] M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, 'Light-absorbing properties, stability, and spectral stabilization of indocyanine green', J. Appl. Physiol., 40, 4, pp. 575-583, 1976. [7] J. Wauters and A. Wilmer, 'Non-invasive liver monitoring in the critically ill: plasma disappearance rate of indocyanine green (ICG-PDR)', Neth. J. Crit. Care., 11, 2, pp. 92-98, 2007. [8] Caesar J, Shaldon S, Chiandussi L, Guevara L, Sherlock S, 'The use of indocyanine green in the measurement of hepatic blood flow and as a test of hepatic function,' Clin. Sci., 21, pp. 43-57, 1961. [9] Vishal Saxena, Mostafa Sadoqi, Jun Shao, 'Degradation Kinetics of Indocyanine Green in Aqueous Solution', J. Pharm. Sci., 92, 10, pp. 2090-2097, 2003. [10] Marvin Minsky, 'Microscopy Apparatus,” U. S. Patent Office, ' 1961. [11] Eugen Hecht, Optics, fourth edition, Pearson Education, Inc., San Francisco, 2002. [12] Chih-Ju Lin, Ning Kang, Jian-Ye Lee, Hsuan-Shu Lee, Chen-Yuan Dong, 'Visualizing and quantifying difference in cytoplasmic and nuclear metabolism in the hepatobiliary system in vivo,' J. Biomed. Opt., 20(1), 2015. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17909 | - |
dc.description.abstract | 循血綠是一種近紅外光的螢光染劑已經被使用在醫學和生物影像領域數十年,吸收峰值約在780奈米,螢光峰值約在805奈米。在臨床醫學上,醫師會給病患注射循血綠,循血綠會經由肝門靜脈進入肝臟,然後被肝細胞代謝經由膽管排出,所以醫師會在注射後15分鐘抽血,量測血液中殘餘的循血綠濃度,用以診斷病患的肝功能。根據組織學,肝腺泡依據功能被區分為三個區域,區域一、區域二和區域三,區域一最接近肝門靜脈最先得到來自腸道的養分,區域三最靠近中央靜脈最遠離腸道養分供給,區域二介於二者之間,在本研究中,我們利用循血綠的特性和反射式共軛焦顯微鏡隨時間拍攝肝腺泡中不同區域的影像。藉由分析這些影像資料,我們發現影像因為光漂白造成強度與對比下降。因此轉而定性上的討論肝細胞排出ICG的速率;更進一步分析肝細胞核與細胞質排出ICG的速率。區域一肝細胞排出ICG的速率最快,區域二次之,區域三最慢。細胞核排出ICG速率大於細胞質排出ICG速率,與區域無關。 | zh_TW |
dc.description.abstract | Indocyanine green (ICG) is a near-infrared fluorescent dye which absorption peak about 780 nm and emission peak about 805 nm. ICG has been used for decades on medical and bio-image field. For example, doctor diagnose a patient’s hepatic function by injecting ICG into the patient and monitor its excretion with time. Since ICG is processed by hepatocytes from portal vein then excreted into the bile duct, diagnosis can be performed by drawing blood from the patient 15 minutes following ICG injection. In this study, we studied ICG metabolism within the liver acinus which is classified into three zones: zone I, zone II, and zone III. Epi-illuminated confocal fluorescence microscope is used with a liver imaging chamber to observe ICG metabolism in vivo. By analyzing ICG liver images found that ICG fluorescence was degraded by excitation light. We discussed ICG excretion rate of hepatocytes to proceed qualitative analysis in three zones. Further, studying nucleus and cytoplasm of individual hepatocyte separately because of the image contrast between nucleus and cytoplasm. In an acinus, zone I hepatocytes excrete ICG fastest. Nucleus excretes ICG fast than cytoplasm independent of zone classified. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T00:45:58Z (GMT). No. of bitstreams: 1 ntu-104-R02245011-1.pdf: 2250723 bytes, checksum: c104c5cb4f59faf142151768a00f848c (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | Abstract i
摘要 ii 目錄 iii 圖目錄 iv 表目錄 vi 第1章 緒論 1 第2章 肝臟組織學 3 2-1肝臟的血液供應與膽道樹 3 2-2 肝臟的構造 6 2-3 肝臟的結構組成 8 2-4 ICG在肝臟中的代謝與臨床肝功能診斷 8 第3章 實驗基礎理論 11 3-1 螢光 11 3-2 螢光顯微鏡 13 3-3 共軛焦顯微鏡 14 3-4 點擴函數與解析度 17 第4章 實驗方法與材料 21 4-1 實驗儀器和架設 21 4-2 實驗動物與手術 23 4-3 使用藥劑 24 4-4 實驗步驟 26 第5章 實驗結果與分析討論 28 5-1 從影像分析肝臟型態 28 5-2 光漂白效應對影像的影響 31 5-3 不同區域間的代謝速率 33 5-4 肝細胞核與細胞質的代謝速率 35 第6章 結論與未來展望 40 參考文獻 41 | |
dc.language.iso | zh-TW | |
dc.title | 利用螢光共軛焦顯微術監控循血綠在小鼠活體肝臟中的代謝 | zh_TW |
dc.title | The Use of Confocal Microscopy to Monitor the Metabolism of Indocyanine Green in Mouse Liver | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳永芳(Yang-Fang Chen),石明豐(Ming-Feng Shih),陳定信(Ding-Shinn Chen),駱遠(Yuan Luo) | |
dc.subject.keyword | 循血綠,螢光,肝細胞代謝,共軛焦顯微鏡, | zh_TW |
dc.subject.keyword | indocyanine green,fluoresence,hepatobiliary metabolism,confocal microscope, | en |
dc.relation.page | 42 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2015-07-31 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 應用物理所 | zh_TW |
顯示於系所單位: | 應用物理研究所 |
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