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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許輔 | |
| dc.contributor.author | Mei-Ju Chen | en |
| dc.contributor.author | 陳美如 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:43:06Z | - |
| dc.date.copyright | 2015-09-17 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-08-13 | |
| dc.identifier.citation | 王竹鑫、章迺榮（民95）。袖珍中藥快速鑑別手冊。長沙：湖南科學技術出版社。
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Effects of Gastrodia elata and its active constituents on scopolamine-induced amnesia in rats. Planta Med. 62, 317-321. Wu, C. R., Hsieh, M. T., Liao, J. (1996b). p-Hydroxybenzyl alcohol attenuates learning deficits in the inhibitory avoidance task: involvement of serotonergic and dopaminergic systems. Chin J. Physiol. 39, 265-273. Xul, Q., Lid, Y., Wang, X., Gu, H., Chen, Z. (1998). Purification and characterization of a novel anti-fungal protein from Gastrodia elata. Plant Physiology Biochemistry. 36(12),899-905. Zhou, B. H., Li, X. J., Liu, M., Wu, Z., Ming, Hu. X. (2006). Antidepressant-like activity of the Gastrodia elata ethanol extract in mice. Fitoterapia . 77, 592-594. Zhu, W., Ma, S., Qu, R., Kang, D. (2006). Antidepressant-like effect of saponins extracted from Chaihu-jia-longgu-muli-tang and its possible mechanism. Life Sci. 79, 749-756. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17759 | - |
| dc.description.abstract | Tianma (Gastrodia elata), also known as fixed wind grass or barbed arrows, is a medicinal orchid used in traditional Chinese medicine to treat vertigo, paralysis, convulsions, cramps, epilepsy, high blood pressure and other symptoms. We have previously purified an immunomodulatory protein IPAF from Anoectochilus formosanus. The protein IPAF has an amino acid sequence highly similar to proteins from Orchidaceae plants, including Epipactis helleborine lectin, Listera ovata lectin, and G. elata-gastrodianin-4B. Therefore, we hypothesized that Orchidaceae plants might possess proteins similar to IPAF. To test our hypothesis, the crude protein was extracted from G. elata, Dendrobium huoshanense, Dendrobium nobile, and Dendrobium loddigesii and then analyzed by polyacrylamide gel electrophoresis and Western blotting. Subsequently, a 15.1 kDa protein G. elata (GEP), which exhibited similar biochemical characteristics to IPAF, was purified via anion exchange chromatography using DE-52 and HiTrap Q columns. Finally, RAW 264.7 murine macrophage cells were used for preliminary activity tests. GEP (5 μg/mL) could activate nitric oxide (NO) (10.4 ± 0.6 μM), TNF-α (935.4 ± 118.5 pg/mL), and IL-1β (25.1 ± 3.0 pg/mL) secretion by RAW 264.7 murine macrophage. In addition, GEP enhanced nucleic acid replication by 1.16-fold as analyzed by BrdU analysis, and promoted 1.43-fold of MTT metabolic activity in murine splenocytes. In this study we purified a new orchid protein, which exhibited similar biochemical characteristics as the IPAF, and we also assessed its immunomodulatory function. GEP activated RAW 264.7 macrophages by enhancing NO, TNF-α and IL-1β secretion. Moreover, GEP also stimulated cell proliferation in mouse splenocytes. On the basis of previous the findings, in our laboratory GEP, as well as other orchid proteins, might play important roles in the medicinal functions of Orchidaceae plants. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:43:06Z (GMT). No. of bitstreams: 1 ntu-104-R00628214-1.pdf: 1577974 bytes, checksum: e6f38d6d4e67172242552035753c95a7 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 誌謝 I
摘要 III Abstract IV 目錄 VI 圖目錄 IX Chapter 1 緒論 1 1.1 序言 1 1.1.1 天麻典故 1 1.1.2 天麻之分部和栽培 1 1.1.3 天麻之傳統藥理與藥用潛力 3 1.1.4 天麻之生理活性 3 1.2 台灣金線連 8 1.3 免疫調節 10 1.3.1 免疫功能 10 1.3.2 發炎反應 11 1.3.3 巨噬細胞的免疫系統角色 12 1.4 研究動機與架構 16 Chapter 2 材料與方法 17 2.1 天麻、霍山石斛、川石斛和金釵石斛等四種蘭科植物粗蛋白製備 17 2.1.1 天麻、霍山石斛、川石斛和金釵石斛等四種蘭科植物粗蛋白萃取 17 2.1.2 天麻、霍山石斛、川石斛和金釵石斛粗蛋白之特性分析 19 2.2 天麻蛋白 GEP 純化 23 2.2.1 DE-52 離子交換樹脂層析 23 2.2.2 快速蛋白質層析系統(FPLC SYSTEM)分析 25 2.3 天麻蛋白 GEP 免疫調節活性試驗 25 2.3.1 一氧化氮 (NITRIC OXIDE) 產量分析 25 2.3.2 Α腫瘤壞死因子 (TNF-α) 產量之分析 27 2.3.3 介白質 (IL-1β) 產量之分析 28 2.3.4 小鼠脾細胞之取得 29 2.3.5 脾臟細胞的代謝活性 (MTT ASSAY) 29 2.3.6 脾臟細胞的細胞增生效應 (BRDU ASSAY) 30 2.3.7 Γ干擾素 (IFN-γ)產量之分析 31 Chapter 3 結果 32 3.1 四種藥用蘭科植物全蛋白萃取及特性分析 32 3.1.1 聚丙烯醯胺膠體電泳分析 32 3.1.2 西方轉漬分析 32 3.2 天麻蛋白 GEP 的純化 33 3.2.1 DE-52陰離子交換樹脂 33 3.2.2 快速蛋白層析系統 (FPLC SYSTEM) 分析 33 3.3 天麻蛋白 GEP 的免疫調節活性 34 3.3.1 天麻蛋白 GEP 對 RAW 264.7 MACROPHAGES 之影響 34 3.3.2 天麻蛋白 GEP 對小鼠脾細胞之影響 38 Chapter 4 討論 42 Chapter 5 結論 45 參考文獻 47 圖表 55 | |
| dc.language.iso | zh-TW | |
| dc.title | 天麻免疫調節蛋白之純化與活性分析 | zh_TW |
| dc.title | Purification and Function Analysis of the Immunomodulatory Protein from Gastrodia elata | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 周志輝,潘敏雄 | |
| dc.subject.keyword | 天麻,純化,免疫調節蛋白, | zh_TW |
| dc.subject.keyword | Gastrodia elata,Purification,Immunomodulatory Protein, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2015-08-13 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
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