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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 洪傳揚 | |
dc.contributor.author | Ling-Hung Yang | en |
dc.contributor.author | 楊翎虹 | zh_TW |
dc.date.accessioned | 2021-06-08T00:43:01Z | - |
dc.date.copyright | 2015-08-28 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-14 | |
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Sci Signal 7. doi: 10.1126/scisignal.2005051. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17753 | - |
dc.description.abstract | 穀胱甘肽還原酶 (Glutathione reductase, GR) 為植物重要的抗氧化酵素,以 NADPH 為基質催化氧化態的 GSSG 還原為 GSH。水稻 GR 基因家族由三個基因組成,分別為位於細胞質的 OsGR2 和葉綠體/粒線體的 OsGR1 與 OsGR3。在 GR1::GUS 轉殖水稻中, GUS 主要表現於種子的胚與糊粉層、莖、葉鞘、葉片、花與根之維管束、側根原基與根尖。西方墨點法分析 OsGR1 對非生物性逆境之反應,發現水稻幼苗地上部OsGR1 為持續性表現,不受非生物逆境與植物荷爾蒙影響,反之,根部 OsGR1 則會受到 ABA、Auxin 與 Cytokinin 誘導表現。利用RNAi 技術弱化 OsGR1 表現,顯示 OsGR1 於地上部與根部分別貢獻40% 與 15% 的總GR 活性。弱化OsGR1 表現後會顯著抑制側根的發育,利用 GSH 專一性螢光探針,monobromobimane (mBBr) 偵測 GSH,發現 GR1-RNAi 轉殖株側根根尖的GSH 含量顯著降低,而外加 GSH 則可回復側根正常的生長。以受 Auxin 誘導之DR5::GUS 轉殖水稻進行組織化學染色分析,結果顯示 GSH 生合成抑制劑buthionine sulfoximine (BSO) 處理會降低側根中 GUS 的表現,並且隨著處理濃度增加抑制效果越顯著。此外,外加 GSH 也可減緩ABA 抑制側根發育與種子的發芽,並回復DR5::GUS 轉殖水稻側根中受ABA 抑制的 GUS 表現,分析ABRC::Luciferase 轉殖水稻,發現 GSH 可抑制 ABA 對ABRC 的誘導。另一方面,大量表現 OsGR1 可降低 ABA 對側根的抑制效果,維持側根根尖 GSH 含量的恆定,並可促進滲透壓逆境下側根的發育。以上結果說明 OsGR1 透過調控側根根尖 GSH 之穩定來調節水稻根部auxin 的轉運,與側根根尖對 ABA 的敏感度,進而調控側根的發育。 | zh_TW |
dc.description.abstract | Glutathione reductases (GRs) are important antioxidant enzymes that catalyze the reduction of glutathione disulfide (GSSG) into reduced glutathione (GSH) with the accompanying oxidation of NADPH. In rice, one cytosolic (OsGR2) and two chloroplast / mitochondria dual-localized GR (OsGR1 and OsGR3) isoforms have been identified. In this study, role of the chloroplast / mitochondria localized OsGR1 was investigated. OsGR1 is primarily expressed in shoots at seedling stage and ubiquitously expressed in all tissues at heading stage. Transgenic rice containing GR1::GUS showed that GUS was expressed in vascular bundle and apical meristem of roots, embryo and aleurone layer of seeds, coleoptile, sheath, stem and leaves. Reduced expression of GR1 by RNA interference (GR1-RNAi) revealed that GR1 had contributed at least 40% and 15% of total GR activity in shoots and roots, respectively. Phenotypic analysis showed that lateral root (LR) growth was strongly inhibited in GR1-RNAi plants. Decreased glutathione levels was observed by GSH specific fluorescent-dye monobromobimane in LR-tip of GR1-RNAi plants, whereas exogenously applied GSH recovered the growth of LR. Treating DR5::GUS transgenic plant, an auxin indicator, with GSH biosynthetic inhibitor buthionine sulfoximine (BSO) showed that a decline in GUS expression was associated with increased BSO concentrations in LR; moreover, exogenously applied GSH relieved the inhibitory effect of ABA on LR development, seed germination, and GUS expression and of DR5::GUS transgenic plants. Through in vivo analysis of luciferase activity in ABRC::Luciferase transgenic rice showed that GSH retards ABA response and signal transduction in rice. In GR1-overexpressing plants, ABA inhibited LR growth was decreased and LR growth was improved under osmotic stress. Taken together, GR1-regulated GSH homeostasis in roots plays a key role in LR development by modulating auxin transport and the sensitivity to ABA. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T00:43:01Z (GMT). No. of bitstreams: 1 ntu-104-R02623003-1.pdf: 5241699 bytes, checksum: f38d60dca21fb07bdc4623ef2a71bc18 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 目錄 口試委員會審定書 致謝 中文摘要..................................i Abstract................................ ii 縮寫字對照表..............................iv 目錄.....................................vi 圖目錄...................................ix 壹、前人研究.............................. 1 一、植物的抗氧化機制........................1 二、植物穀胱甘肽 (Glutathione, GSH) 的生理角色....2 2.1 穀胱甘肽之簡介...............................2 2.2 穀胱甘肽之生合成途徑..........................2 2.3 穀胱甘肽參與調控細胞周期......................3 2.4 穀胱甘肽影響植物生長發育......................3 三、Glutathione reductase (GR) 的特性與生理功能......5 3.1 GR的構造.......................................5 3.2 植物 GR的生理功能...............................5 3.3 水稻 GR 基因家族與生理功能.......................6 四、側根之發育與調控.................................6 4.1 植物荷爾蒙交互作用調控側根發育....................7 4.2 ABA 拮抗 auxin 並抑制側根發育...................7 4.3 水稻側根發育之研究...............................8 貳、本論文研究目的及實驗架構..........................9 參、材料與方法......................................10 一、植物材料準備及生長條件...........................10 二、質粒的構築......................................11 三、水稻基因轉殖....................................14 四、基因表現分析材料準備及處理........................16 五、基因表現分析....................................19 六、水稻試驗處理及生理分析...........................19 七、OsGR1啟動子特性分析.............................25 八、 側根生長素分布與累積分析........................25 九、Firefly Luciferase 活性分析....................25 十、統計分析.......................................26 肆、結果...........................................27 一、OsGR1 分子特性分析..............................27 二、OsGR1 轉殖株的功能分析..........................28 三、OsGR1 調控側根發育的機制.........................30 四、OsGR1 參與調控滲透壓逆境下側根的發育...............32 五、 OsGR1大量表現與弱化表現轉殖株中參與ABA生合成與下游訊息傳遞之基因表現...........................................33 六、 OsGR1 調控水稻側根發育模式圖......................33 伍、討論.............................................34 一、 OsGR1 分子特性分析...............................34 二、 水稻 GR 同功酶對於總GR活性的貢獻...................34 三、 水稻 GR 與根部發育................................35 四、 OsGR1 影響側根根尖 GSH 庫恆定是影響側根發育的主因....37 五、 OsGR1 透過維持側根根尖GSH 庫恆定影響 Auxin 分布.....38 六、 地上部 GSH/GSSG 失衡可能影響根部的發育..............39 七、 ABA 調控 auxin 運輸與訊息傳遞並抑制側根發育..........39 八、 ABA 與 GSH pool調控的上下游關係.....................40 九、 外加 GSH 與大量表現 OsGR1降低水稻對ABA敏感度..........40 十、 逆境下 OsGR1 調控GSH 恆定與 ABA 反應影響水稻側根發育與耐受性的評估..............................................42 陸、參考文獻............................................43 柒、 附錄..............................................86 一、 水稻基因轉殖用培養基列表............................ 86 二、 木村氏 (Kimura) 水耕液配方..........................88 三、 原態膠體電泳分析....................................91 四、 變性膠體電泳分析....................................92 五、 GUS 染色溶液配方...................................94 六、 試驗所使用的載體....................................94 七、 本論文中基因表現分析所使用的引子列表..................95 八、 Redox-sensitive GFP (roGFP) 與 GR1tp-roGFP 的次細胞定位.....................................................96 | |
dc.language.iso | zh-TW | |
dc.title | 水稻穀胱甘肽還原酶 1 調控側根發育之研究 | zh_TW |
dc.title | Studies on the Role of Glutathione Reductase 1 in the Regulation of Lateral Root Development in Rice (Oryza sativa L.) | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 賀端華,葉國楨,張孟基,蔡育彰 | |
dc.subject.keyword | 穀胱甘?還原?,穀胱甘?,側根發育,生長素,離層酸,水稻, | zh_TW |
dc.subject.keyword | Glutathione reductase,Glutathione,Lateral root development,Auxin,Abscisic acid,Rice (Oryza sativa L.), | en |
dc.relation.page | 96 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2015-08-14 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
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