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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳昭瑩 | |
| dc.contributor.author | Yun-Ting Ou | en |
| dc.contributor.author | 歐昀庭 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:18:10Z | - |
| dc.date.copyright | 2013-07-31 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-07-26 | |
| dc.identifier.citation | 1. 羅秋雄。2005。作物施肥手冊。增修六版。台北：行政院農業委員會農糧署。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17520 | - |
| dc.description.abstract | 甘藍黑腐病造成甘藍的減產，一直以來都是難以解決的問題。本研究測試生物防治方法，期能提供目前既有防治選項外的另一可行方案，以減輕黑腐病對甘藍的危害。首先以誘導系統抗性能力為篩選指標，同時搭配甘藍苗生長影響作綜合性評估，得知菌株28-5、AA2-3、37-1與M-2最具應用潛力。以此四個菌株進行田間應用試驗，比較涵蓋甘藍外部與內部病徵之罹病程度，顯示菌株37-1之生物防治效果最佳。在測試各菌株葉面保護能力時，菌株37-1之保護率亦最佳，故針對菌株37-1進行ISR與葉面保護特性之探討，包括菌株37-1之最適施用濃度。在病徵發展方面，根圈施用菌株37-1再接種病原細菌，12天後的罹病度與對照組之差異最為明顯，而葉內病原族群則於接種後7~9天呈現變化；觀察葉緣水孔癒傷葡聚醣累積情形，發現菌株37-1處理的甘藍植株於接種後第5天呈現與對照組不同的變化趨勢，顯示此菌株可經由誘導系統抗性之機制抑制甘藍黑腐病之病徵發展。 | zh_TW |
| dc.description.abstract | Reduced production of cabbages caused by black rot has been a problem difficult to solve. In this study, biocontrol method as an alternative besides the present disease control measures was investigated to reduce the economic loss caused by cabbage black rot. At beginning, induced systemic resistance (ISR) of plants was used as a main screening index of potential biocontrol agents. In combination with the results of seedling growth, strains 28-5, AA2-3, 37-1 and M-2 showed good potentials for application. Field trial with these four strains showed that strain 37-1 had the best biocontrol activity according to the external and internal symptoms. On the other hand, strain 37-1 exhibited superior activity in foliar protection. Thus, the properties of ISR and foliar protection of strain 37-1 were further investigated, including the suitable concentration of application. In the aspect of disease development, disease severity of the plants pre-drenched with strain 37-1 were significantly reduced at 12 days after pathogen inoculation as compared with the control without bacterization. In addition, the pathogen populations in cabbage leaves fluctuated at 7 and 9 days post pathogen inoculation following drench application of strain 37-1. The examination of callose deposition on hydathode areas at leaf margin indicated the seedlings of cabbage drenched with strain 37-1 had defense reaction as compared with the control at 5 days after pathogen inoculation. Thus, drench application of strain 37-1 could suppress symptom development of cabbage black rot by a mechanism of ISR. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:18:10Z (GMT). No. of bitstreams: 1 ntu-102-R99633012-1.pdf: 1073885 bytes, checksum: cbc49c5429f7bbc7de4d6935bb3792c9 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 目錄
壹、中文摘要 1 貳、英文摘要 2 參、前言 3 肆、前人研究 5 一、甘藍黑腐病 5 二、植物病害生物防治 7 三、芽孢桿菌屬菌株於生物防治之應用潛力 8 四、芽孢桿菌屬菌株防治甘藍黑腐病之研究 10 伍、材料與方法 11 一、供試菌株之培養與保存 11 二、各芽孢桿菌屬菌株對甘藍之ISR作用 11 1. 甘藍黑腐病罹病嚴重度與統計方式 11 2. 室外網室初步篩選試驗 11 3. 植物生長室試驗 12 三、各芽孢桿菌屬菌株對甘藍幼苗生長之影響試驗 13 1. 植物生長室試驗 13 2. 無菌栽培試驗 13 四、通過篩選之四個菌株的田間試驗 14 1. 植物生長室植株育苗 14 2. 植株生長試驗 14 3. 病害防治試驗 15 五、各芽孢桿菌屬菌株對甘藍之葉面保護能力測試 15 1. 植物生長室試驗之植株栽培 15 2. 甘藍葉面保護試驗 15 六、各芽孢桿菌屬菌株拮抗甘藍黑腐病菌能力測試 16 七、菌株37-1對甘藍之葉面保護能力測試 16 1. 植物生長室試驗之植株栽培 16 2. 不同濃度菌液對甘藍之葉面保護 16 八、菌株37-1對甘藍之ISR作用測試 17 1. 植物生長室試驗之植株栽培 17 2. 不同濃度菌液對甘藍之ISR 17 九、菌株37-1對甘藍葉內黑腐病菌族群量影響試驗 17 1. 植物生長室試驗之植株栽培 17 2. 甘藍葉之黑腐病菌族群計量 17 十、菌株37-1對甘藍葉片癒傷葡聚醣累積影響試驗 18 1. 噴霧接種試驗 18 2. 注射接種試驗 19 陸、結果 20 一、各芽孢桿菌屬菌株對甘藍之ISR作用 20 1. 室外網室初步篩選試驗 20 2. 各芽孢桿菌屬菌株於不同處理時間對甘藍之ISR作用(植物生長室試驗) 20 二、各芽孢桿菌屬菌株對甘藍幼苗之生長影響 21 1. 各芽孢桿菌屬菌株懸浮液對甘藍幼苗生長之影響 21 2. 各芽孢桿菌屬菌株懸浮液對甘藍幼苗根部生長之影響 21 三、各芽孢桿菌屬菌株之綜合特性比較 21 四、芽孢桿菌屬菌株之田間試驗 22 五、各芽孢桿菌屬菌株對甘藍之葉面保護能力 22 六、各芽孢桿菌屬菌株拮抗甘藍黑腐病菌能力 23 七、菌株37-1對甘藍之葉面保護能力 23 八、菌株37-1對甘藍之ISR作用 24 九、菌株37-1對甘藍葉內黑腐病菌族群量影響 24 十、菌株37-1對甘藍葉片癒傷葡聚醣累積影響 24 1. 噴霧接種試驗 24 2. 注射接種試驗 25 柒、討論 26 捌、參考文獻 31 玖、圖表集 46 表一、芽孢桿菌屬菌株ISR特性綜合比較 47 圖一、各芽孢桿菌屬菌株對甘藍之ISR 作用(初步篩選) 48 圖二、各芽孢桿菌屬菌株對甘藍之ISR 作用(澆灌方式一) 49 圖三、各芽孢桿菌屬菌株對甘藍之ISR 作用(澆灌方式二) 50 圖四、各芽孢桿菌屬菌株對甘藍幼苗生長之影響 52 圖五、各芽孢桿菌屬菌株對甘藍幼苗根部生長之影響 53 圖六、田間試驗之芽孢桿菌屬菌株施用與黑腐病菌接種時間 54 圖七、田間甘藍黑腐病罹病度計量方式 55 圖八、田間測試芽孢桿菌屬菌株對甘藍黑腐病病徵發展之影響 56 圖九、田間測試芽孢桿菌屬菌株防治甘藍黑腐病效果 59 圖十、田間測試芽孢桿菌屬菌株對甘藍葉球產量之影響 60 圖十一、田間甘藍黑腐病防治試驗期間之溫度雨量圖 61 圖十二、各芽孢桿菌屬菌株對甘藍黑腐病之葉面防治效果 62 圖十三、菌株37-1菌液的界面活性 63 圖十四、各芽孢桿菌屬菌株在LB培養基上對黑腐病菌之拮抗作用 65 圖十五、不同濃度菌株37-1之葉面防治效果 66 圖十六、不同濃度菌株37-1之ISR作用 67 圖十七、菌株37-1對甘藍幼苗葉內黑腐病菌族群影響 68 圖十八、菌株37-1對噴霧接種黑腐病菌之甘藍葉部癒傷葡聚醣累積影響 70 圖十九、菌株37-1對注射接種黑腐病菌之甘藍葉部癒傷葡聚醣累積影響 71 | |
| dc.language.iso | zh-TW | |
| dc.title | 甘藍黑腐病之生物防治研究 | zh_TW |
| dc.title | Biocontrol study of cabbage black rot | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭安秀,沈偉強,黃祥恩 | |
| dc.subject.keyword | 生物防治菌,甘藍,黑腐病,誘導系統抗性,葉面保護, | zh_TW |
| dc.subject.keyword | Biocontrol agent,cabbage,black rot disease,induced systemic resistance (ISR),foliar protection, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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