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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳美如(Mei-Ru Chen) | |
| dc.contributor.author | Tsung-Yu Yang | en |
| dc.contributor.author | 楊宗諭 | zh_TW |
| dc.date.accessioned | 2021-06-08T00:10:57Z | - |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-06 | |
| dc.identifier.citation | 黃浩雲，(2011)，EB 病毒BGLF4 蛋白質激酶與IQGAP1 的交互作用及其對於
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17402 | - |
| dc.description.abstract | Epstein-Barr virus (EBV) is the first human virus to be implicated in carcinogenesis. Over 90 percent of people are infected by EBV in the world. Upon lytic replication, the nucleocapsids of EBV need to be translocated from the nucleus into cytoplasm for tegumentation and final maturation after DNA replication and encapsidation. Little is known about how EBV remodels cytoskeleton and cellular secretory apparatuses for efficient cytoplasmic assembly. Using EBV infected nasopharyngeal carcinoma NA cells we showed that, cells displayed modified nuclear structure after EBV reactivation. Viral capsid, tegument proteins and glycoproteins are clustered at juxtanuclear concave region accompanied by redistributed cytoplasmic organelles, MTOC and cytoskeleton regulator IQGAP1, suggesting EBV builds a specialized cytoplasmic assembly compartment for an efficient cytoplasmic maturation process. This compartment was disrupted after siRNA knockdown of BGLF4 kinase. Transient expression of BGLF4 induced a compact Golgi structure along with reorganization of MTOC and IQGAP1, indicating that BGLF4 plays a critical role for assembly compartment formation and cytoskeleton rearrangement. Treatment with microtubule-disturbing agents in cells inhibited BGLF4 induced compact Golgi structure, suggesting BGLF4 induces assembly compartment formation through regulation of cytoskeleton rearrangement. Since BGLF4 interacted with IQGAP1 in co-immunoprecipitation assay and
phosphorylated IQGAP1 in vitro,it is possible that BGLF4 regulates cytoskeleton through phosphorylation of IQGAP1. While viral DNA replication and maturation was enhanced in IQGAP1 knockdown cells, IQGAP1 may serve as a negative regulator of EBV lytic cycle. Taken together, I propose that BGLF4 induces cytoskeleton rearrangement and instability through phosphorylation of IQGAP1, and subsequently facilitates assembly compartment formation and virus maturation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T00:10:57Z (GMT). No. of bitstreams: 1 ntu-102-R00445104-1.pdf: 19697251 bytes, checksum: 2a0d3a69e08fe3f07f6a2423d503828e (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 圖次 ......................................................................................................... ii
中文摘要 ................................................................................................ iv 英文摘要 ................................................................................................. v 緒論 ......................................................................................................... 1 材料與方法 ........................................................................................... 14 結果 ....................................................................................................... 27 討論 ....................................................................................................... 35 圖 ........................................................................................................... 41 參考文獻 ............................................................................................... 66 | |
| dc.language.iso | zh-TW | |
| dc.title | EB 病毒 BGLF4 透過調控細胞骨架以協助病毒細胞質組裝區域之形成 | zh_TW |
| dc.title | EBV BGLF4 Induces Formation of Viral Cytoplasmic Assembly Compartment Through Rearrangement of Cytoskeleton | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林素芳(Su-Fang Lin),張雯(Wen Chang),李重霈(Chung-Pei Lee) | |
| dc.subject.keyword | EB 病毒,病毒細胞質組裝區域,BGLF4,IQGAP1,細胞骨架,微管,高基氏體, | zh_TW |
| dc.subject.keyword | Epstein-Barr virus (EBV),cytoplasmic assembly compartment,BGLF4,IQGAP1,cytoskeleton,microtubule,Golgi apparatus, | en |
| dc.relation.page | 72 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-08-07 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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