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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
dc.contributor.author | Hsien-Jen Kuo | en |
dc.contributor.author | 郭獻仁 | zh_TW |
dc.date.accessioned | 2021-06-08T00:04:21Z | - |
dc.date.copyright | 2013-08-27 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-14 | |
dc.identifier.citation | 1 Craft, D. L., Madduri, K. M., Eshoo, M. & Wilson, C. R. Identification and characterization of the CYP52 family of Candida tropicalis ATCC 20336, important for the conversion of fatty acids and alkanes to alpha,omega-dicarboxylic acids. Applied and environmental microbiology 69, 5983-5991 (2003).
2 Eschenfeldt, W. H. et al. Transformation of fatty acids catalyzed by cytochrome P450 monooxygenase enzymes of Candida tropicalis. Applied and environmental microbiology 69, 5992-5999 (2003). 3 Giri, R. & Yu, J. Q. Synthesis of 1,2- and 1,3-dicarboxylic acids via Pd(II)-catalyzed carboxylation of aryl and vinyl C-H bonds. Journal of the American Chemical Society 130, 14082-14083 (2008). 4 Picataggio, S. et al. Metabolic engineering of Candida tropicalis for the production of long-chain dicarboxylic acids. Biotechnology (N Y) 10, 894-898 (1992). 5 http://www.docin.com/p-342469183.html. 6 Samorski, M. & Dierker, M. WO 2010020368. World Patent (2010). 7 Van Beilen, J. B. & Funhoff, E. G. Alkane hydroxylases involved in microbial alkane degradation. Appl Microbiol Biotechnol 74, 13-21 (2007). 8 Sharma, S. L. & Pant, A. Biodegradation and conversion of alkanes and crude oil by a marine Rhodococcus sp. Biodegradation 11, 289-294 (2000). 9 Rojo, F. Degradation of alkanes by bacteria. Environmental microbiology 11, 2477-2490 (2009). 10 Sumita, T. et al. Peroxisome deficiency represses the expression of n-alkane-inducible YlALK1 encoding cytochrome P450ALK1 in Yarrowia lipolytica. FEMS microbiology letters 214, 31-38 (2002). 11 Spencer, J. F., Ragout de Spencer, A. L. & Laluce, C. Non-conventional yeasts. Appl Microbiol Biotechnol 58, 147-156 (2002). 12 Scheller, U., Zimmer, T., Becher, D., Schauer, F. & Schunck, W. H. Oxygenation cascade in conversion of n-alkanes to alpha,omega-dioic acids catalyzed by cytochrome P450 52A3. The Journal of biological chemistry 273, 32528-32534 (1998). 13 Cheng, Q. et al. Candida yeast long chain fatty alcohol oxidase is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism. Biochimica et biophysica acta 1735, 192-203 (2005). 14 Sanglard, D., Kappeli, O. & Fiechter, A. Metabolic conditions determining the composition and catalytic activity of cytochrome P-450 monooxygenases in Candida tropicalis. Journal of bacteriology 157, 297-302 (1984). 15 Sumita, T. et al. YlALK1 encoding the cytochrome P450ALK1 in Yarrowia lipolytica is transcriptionally induced by n-alkane through two distinct cis-elements on its promoter. Biochemical and biophysical research communications 294, 1071-1078 (2002). 16 Gmunder, F. K., Kappeli, O. & Fiechter, A. Chemostat studies on the hexadecane assimilation by the yeast Candida tropicalis. European J. Appl. Microbiol. Biotechnol. 12, 135-142 (1981). 17 Seghezzi, W. et al. Identification and characterization of additional members of the cytochrome P450 multigene family CYP52 of Candida tropicalis. DNA and cell biology 11, 767-780 (1992). 18 Scheller, U., Kraft, R., Schroder, K. L. & Schunck, W. H. Generation of the soluble and functional cytosolic domain of microsomal cytochrome P450 52A3. The Journal of biological chemistry 269, 12779-12783 (1994). 19 Sutter, T. R., Sanglard, D. & Loper, J. C. Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins. The Journal of biological chemistry 265, 16428-16436 (1990). 20 Vogel, F., Gengnagel, C., Kargel, E., Muller, H. G. & Schunck, W. H. Immunocytochemical localization of alkane-inducible cytochrome P-450 and its NADPH-dependent reductase in the yeast Candida maltosa. European journal of cell biology 57, 285-291 (1992). 21 Zimmer, T., Kaminski, K., Scheller, U., Vogel, F. & Schunck, W. H. In vivo reconstitution of highly active Candida maltosa cytochrome P450 monooxygenase systems in inducible membranes of Saccharomyces cerevisiae. DNA and cell biology 14, 619-628 (1995). 22 Smit, M. S., Mokgoro, M. M., Setati, E. & Nicaud, J. M. alpha,omega-Dicarboxylic acid accumulation by acyl-CoA oxidase deficient mutants of Yarrowia lipolytica. Biotechnology letters 27, 859-864 (2005). 23 Ohkuma, M. et al. CYP52 (cytochrome P450alk) multigene family in Candida maltosa: identification and characterization of eight members. DNA and cell biology 14, 163-173 (1995). 24 Ohkuma, M., Tanimoto, T., Yano, K. & Takagi, M. CYP52 (cytochrome P450alk) multigene family in Candida maltosa: molecular cloning and nucleotide sequence of the two tandemly arranged genes. DNA and cell biology 10, 271-282 (1991). 25 Van Beilen, J. B. & Funhoff, E. G. Expanding the alkane oxygenase toolbox: new enzymes and applications. Current opinion in biotechnology 16, 308-314 (2005). 26 Van Beilen, J. B. et al. Cytochrome P450 alkane hydroxylases of the CYP153 family are common in alkane-degrading eubacteria lacking integral membrane alkane hydroxylases. Applied and environmental microbiology 72, 59-65 (2006). 27 Takagi, M., Moriya, K. & Yano, K. Induction of cytochrome P450 in petroleum-assimilating yeast--I. selection of a strain and basic characterization of cytochrome P450 induction in the strain. Cellular and molecular biology, including cyto-enzymology 25, 363-369 (1979). 28 Liu, S. C., Xie, L. Y., Li, C. & Cao, Z. A. Measurement of intracellular pH in long-chain dicarboxylic acid-producing yeast Candida tropicalis and its growth activity. Sheng wu gong cheng xue bao = Chinese journal of biotechnology 20, 279-283 (2004). 29 Mauersberger, S., Matyashova, R. N., Muller, H. G. & Losinov, A. B. Influence of the growth substrate and the oxygen concentration in the medium on the cytochrome P-450 content in Candida guilliermondii. European J. Appl. Microbiol. Biotechnol. 9, 285-294 (1980). 30 Iida, T., Sumita, T., Ohta, A. & Takagi, M. The cytochrome P450ALK multigene family of an n-alkane-assimilating yeast, Yarrowia lipolytica: cloning and characterization of genes coding for new CYP52 family members. Yeast 16, 1077-1087 (2000). 31 Gilewicz, M., Zacek, M., Bertrand, J. C. & Azoulay, E. Hydroxylase regulation in Candida tropicalis grown on alkanes. Canadian journal of microbiology 25, 201-206 (1979). 32 Mauersberger, S., Schunck, W. H. & Muller, H. H. The induction of cytochrome P-450 in Lodderomyces elongisporus. Zeitschrift fur allgemeine Mikrobiologie 21, 313-321 (1981). 33 Sanglard, D., Kappeli, O. & Fiechter, A. The distinction of different types of cytochromes P-450 from the yeasts Candida tropicalis and Saccharomyces uvarum. Archives of biochemistry and biophysics 251, 276-286 (1986). 34 Bernhardt, R. Cytochromes P450 as versatile biocatalysts. Journal of biotechnology 124, 128-145 (2006). 35 Ratajczak, A., Geissdorfer, W. & Hillen, W. Alkane hydroxylase from Acinetobacter sp. strain ADP1 is encoded by alkM and belongs to a new family of bacterial integral-membrane hydrocarbon hydroxylases. Applied and environmental microbiology 64, 1175-1179 (1998). 36 Tani, A., Ishige, T., Sakai, Y. & Kato, N. Gene structures and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1. Journal of bacteriology 183, 1819-1823 (2001). 37 Watkinson, R. J. & Morgan, P. Physiology of aliphatic hydrocarbon-degrading microorganisms. Biodegradation 1, 79-92 (1990). 38 Whyte, L. G. et al. Biodegradation of variable-chain-length alkanes at low temperatures by a psychrotrophic Rhodococcus sp. Applied and environmental microbiology 64, 2578-2584 (1998). 39 Maeng, J. H., Sakai, Y., Tani, Y. & Kato, N. Isolation and characterization of a novel oxygenase that catalyzes the first step of n-alkane oxidation in Acinetobacter sp. strain M-1. Journal of bacteriology 178, 3695-3700 (1996). 40 Wentzel, A., Ellingsen, T. E., Kotlar, H. K., Zotchev, S. B. & Throne-Holst, M. Bacterial metabolism of long-chain n-alkanes. Appl Microbiol Biotechnol 76, 1209-1221 (2007). 41 Maier, T., Forster, H. H., Asperger, O. & Hahn, U. Molecular characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104. Biochemical and biophysical research communications 286, 652-658 (2001). 42 Feng, L. et al. Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. Proceedings of the National Academy of Sciences of the United States of America 104, 5602-5607 (2007). 43 Ratledge, C. Microbial conversions of n-alkanes to fatty acids: A new attempt to obtain economical microbial fats and fatty acids. Chemistry & industry 26, 843-854 (1970). 44 Fickers, P. et al. Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS yeast research 5, 527-543 (2005). 45 Lasserre, J. P. et al. First complexomic study of alkane-binding protein complexes in the yeast Yarrowia lipolytica. Talanta 80, 1576-1585 (2010). 46 Kappeli, O., Muller, M. & Fiechter, A. Chemical and structural alterations at the cell surface of Candida tropicalis, induced by hydrocarbon substrate. Journal of bacteriology 133, 952-958 (1978). 47 Cirigliano, M. C. & Carman, G. M. Isolation of a bioemulsifier from Candida lipolytica. Applied and environmental microbiology 48, 747-750 (1984). 48 Roy, P. K., Singh, H. D., Bhagat, S. D. & Baruah, J. N. Characterization of hydrocarbon emulsification and solubilization occurring during the growth of Endomycopsis lipolytica on hydrocarbons. Biotechnology and Bioengineering 21, 955-974 (1979). 49 Reisfeld, A., Rosenberg, E. & Gutnick, D. Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures. Applied microbiology 24, 363-368 (1972). 50 Hettema, E. H. & Tabak, H. F. Transport of fatty acids and metabolites across the peroxisomal membrane. Biochimica et biophysica acta 1486, 18-27 (2000). 51 Beopoulos, A., Chardot, T. & Nicaud, J. M. Yarrowia lipolytica: A model and a tool to understand the mechanisms implicated in lipid accumulation. Biochimie 91, 692-696 (2009). 52 Cao, Z., Gao, H., Liu, M. & Jiao, P. Engineering the acetyl-CoA transportation system of Candida tropicalis enhances the production of dicarboxylic acid. Biotechnology journal 1, 68-74 (2006). 53 Ueda, M., Tanaka, A. & Fukui, S. Characterization of peroxisomal and mitochondrial carnitine acetyltransferases purified from alkane-grown Candida tropicalis. European journal of biochemistry / FEBS 138, 445-449 (1984). 54 Rottensteiner, H. & Theodoulou, F. L. The ins and outs of peroxisomes: co-ordination of membrane transport and peroxisomal metabolism. Biochimica et biophysica acta 1763, 1527-1540 (2006). 55 Okazaki, K. et al. Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence. Proceedings of the National Academy of Sciences of the United States of America 83, 1232-1236 (1986). 56 Okazaki, K., Tan, H., Fukui, S., Kubota, I. & Kamiryo, T. Peroxisomal acyl-coenzyme A oxidase multigene family of the yeast Candida tropicalis; nucleotide sequence of a third gene and its protein product. Gene 58, 37-44 (1987). 57 Picataggio, S., Deanda, K. & Mielenz, J. Determination of Candida tropicalis acyl coenzyme A oxidase isozyme function by sequential gene disruption. Molecular and cellular biology 11, 4333-4339 (1991). 58 Nicaud, J. M., Thevenieau, F., Le Dall, M. T. & Marchall, R. WO 2006064131. World Patent (2006). 59 Picataggio, S., Deanda, K. & Eirich, L. D. WO 9106660. World Patent (1993). 60 Functional Expression of the Alkane-Inducible Monooxygenase System of the Yeast: Candida tropicalis in Saccharomyces cerevisiae. Biocatalysis and Biotransformation 4, 19-28 (1990). 61 Schunck, W. H. et al. Comparison of two cytochromes P-450 from Candida maltosa: primary structures, substrate specificities and effects of their expression in Saccharomyces cerevisiae on the proliferation of the endoplasmic reticulum. European journal of cell biology 55, 336-345 (1991). 62 Jiao, P., Ma, S., Hua, Y., Huang, Y. & Cao, Z. Isolation and enzyme determination of Candida tropicalis mutants for DCA production. The Journal of general and applied microbiology 46, 245-249 (2000). 63 Yi, Z.-H. & Rehm, H.-J. Metabolic formation of dodecanedioic acid from n-dodecane by a mutant of Candida tropicalis. European J. Appl. Microbiol. Biotechnol. 14, 254-258 (1982). 64 Yi, Z.-H. & Rehm, H.-J. Bioconversion of elaidic acid to Δ9-trans-1,18-octadecenedioic acid by Candida tropicalis. Applied Microbiology and Biotechnology 29, 305-309 (1988). 65 Fabritius, D., Schafer, H. J. & Steinbuchel, A. Identification and production of 3-hydroxy-Δ9-cis-1,18-octadecenedioic acid by mutants of Candida tropicalis. Applied Microbiology and Biotechnology 45, 342-348 (1996). 66 Liu, S., Li, C., Xie, L. & Cao, Z. Intracellular pH and metabolic activity of long-chain dicarylic acid-producing yeast Candida tropicalis. Journal of bioscience and bioengineering 96, 349-353 (2003). 67 Yang, Y. et al. Two-step biocatalytic route to biobased functional polyesters from omega-carboxy fatty acids and diols. Biomacromolecules 11, 259-268 (2010). 68 Liu, S., Li, C., Fang, X. & Cao, Z. Optimal pH control strategy for high-level production of long-chain [alpha], [omega] -dicarboxylic acid by Candida tropicalis. Enzyme Microb. Technol. 34, 73-77 (2004). 69 Hahn-Hagerdal, B. et al. Role of cultivation media in the development of yeast strains for large scale industrial use. Microbial cell factories 4, 31 (2005). 70 Fabritius, D., Schafer, H. J. & Steinbuchel, A. Bioconversion of sunflower oil, rapeseed oil and ricinoleic acid by Candida tropicalis M25. Applied Microbiology and Biotechnology 50, 573-578 (1998). 71 Anderson, K. W. & Wenzel, J. D. US 6569670. United States Patent (2003). 72 Kunishige, E. & Morinaga, T. US 4474882. United States Patent (1984). 73 Gangopadhyay, S., Nandi, S. & Ghosh, S. Biooxidation of fatty acid distillates to dibasic acids by a mutant of Candida tropicalis. Journal of oleo science 56, 13-17 (2006). 74 Akabori, S. & Uchio, R. US 3843466. United States Patent (1974). 75 Yi, Z.-H. & Rehm, H.-J. Formation and degradation of Δ9-1,18-octadecenedioic acid from oleic acid by Candida tropicalis. Applied Microbiology and Biotechnology 28, 520-526 (1988). 76 Kozak, W., Rebrovic, L., Gottman, A. M. & Staley, M. D. WO 0121572. World Patent (2001). 77 Mobley, D. P. Biosynthesis of long-chain dicarboxylic acid monomers from renewable resources - Final technical report. GE Corporate Research and Development One Research Circle Niskayuna, NY 12309 (USA) (1999). 78 Uemura, N. Production of dicarboxylic acids by fermentation. J. Am. Oil Chem. Soc. 64, 1254-1254 (1987). 79 Anderson, K. W., Wenzel, J. D., Fayter, R. G. & McVay, K. R. WO 2000015828. World Patent (2000). 80 http://cathaybiotech.com. 81 http://www.stdaily.com/kjrb/ content/2010-03/12/content_164082.htm. 82 Winston, F. EMS and UV Mutagenesis in Yeast. Current Protocols in Molecular Biology (2008). 83 郭政寬. 利用碳氫化合物為原料以醱酵法生產長鏈二質子羧酸之研究. 國立臺灣大學碩士論文 (1988). 84 Huf, S., Krugener, S., Hirth, T., Rupp, S. & Zibek, S. Biotechnological synthesis of long-chain dicarboxylic acids as building blocks for polymers. European Journal of Lipid Science and Technology 113, 548-561, doi:10.1002/ejlt.201000112 (2011). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17274 | - |
dc.description.abstract | 許多微生物具有將長鏈正烷類或脂肪酸轉化為二元酸之能力,二元酸轉化平台之相關研究及產程開發是以熱帶假絲酵母 (Candida tropicalis) 為主。本研究以熱帶假絲酵母 (Candida tropicalis) ATCC 20962,進行十二碳二元酸 (DCA12) 之醱酵轉化研究,該醱酵策略分為菌體累積及正十二烷 (n-dodecane) 轉化為二元酸兩個階段。在第一階段之菌體累積,分別於醱酵槽比較批式饋料培養 (fed-batch culture) 與批式培養 (batch culture) 對正十二烷之轉化效率。批式培養後經 96 小時之轉化可得到 65 g/L 之 DCA12,而批式饋料培養結果顯示,單以葡萄糖作為碳源之饋料培養基,無法有效提升生菌數,而饋料培養基中添加酵母抽出物與蛋白腖等有機氮源後,雖能有效提升生菌數,但無 DCA12 產出。當以無機銨鹽作為氮源後此現象獲得改善,經 72 小時之轉化可得到 99 g/L 之 DCA12,產率為 1.37 g/L/h。此外,在第二階段 (轉化階段) 維持高生菌數是獲得十二碳二元酸高產量的必要條件。將菌體濃度提升至批式培養之 3 倍後,並調整葡萄糖供給速率及添加少量微量元素和維生素,經 48 小時之轉化可得到 87.8 g/L 之 DCA12,產率達 1.82 g/L/h。進一步以相同策略將菌體濃度提升至批式培養之 5 倍後,經 158.75 小時之轉化 DCA12 最高濃度可達 130.8 g/L,產率為 0.82 g/L/h。後者 DCA12 產率低於前者,推測為培養環境之溶氧濃度過低 (前者約 40% 飽和度,後者約 20% 飽和度),限制了十二碳二元酸之轉化效率。菌種改良方面,藉由紫外線照射導致菌株突變後,於搖瓶中培養並篩選二元酸高產量菌株,可獲得產量增加一倍之突變株。 | zh_TW |
dc.description.abstract | Many microorganisms are able to convert n-alkanes and fatty acids into long chain dicarboxylic acids (DCA), and many researches and bioprocesses using C. tropicalis to produce long chain DCA have been proposed. In this study, the bioprocess is generally divided into two stages, the growth of C. tropicalis ATCC 20962 and bioconversion of n-dodecane (alkane C12) into dodecanedioic acid (DCA12). To get higher DCA productivity, it is required to increase the number of viable cells and bioconversion ability with optimized fermentation conditions. We compared batch culture with fed-batch culture about the accumulation of viable cells and bioconversion of n-dodecane. The production of DCA12 reached 65 g/L in batch culture with 96 h bioconversion. In fed-batch culture, fed medium containing only carbon source (glucose) cannot raise the number of viable cells efficiently. Addition of organic nitrogen source (yeast extract and peptone) in fed medium can effectively increase the number of viable cells, but the cells cannot produce DCA12. Instead, adding inorganic ammonium salts as nitrogen source in fed medium can make 3-fold increase of viable cell compared with batch culture and raise DCA12 production to 99 g/L in 72 h bioconversion (1.37 g/L/h of productivity). Moreover, it is necessary to maintain the large number of viable cells in bioconversion phase for higher DCA production. By adjusting the feeding rate of glucose with supplemental trace elements and vitamins in bioconversion phase, the production of DCA12 reached 87.8 g/L after 48 h bioconversion (1.82 g/L/h of productivity) from 3-fold viable cells. By the same fed-batch strategy to get 5-fold increased of viable cells, the production of DCA12 reached 130.8 g/L after 158.75 h bioconversion (0.82 g/L/h of productivity). We assumed that the major limiting factor of DCA12 productivity is dissolved oxygen concentration in culture medium (the former is 40% and the latter is 20%). For strain improvement, one strain mutated by UV-irradiation has been selected after screening to have 2-fold DCA12 productivity in flask culture. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T00:04:21Z (GMT). No. of bitstreams: 1 ntu-102-R00b22047-1.pdf: 4979863 bytes, checksum: 792387ae4e9cdc224a1559687243a9c7 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 I
謝誌 II 中文摘要 III Abstract IV 縮寫表 V 目錄 VI 圖表目錄 IX 壹、緒論 IX 1.1 二元酸 1 1.1.1 二元酸之用途及來源 1 1.1.2 二元酸之市場規模 1 1.2 十二碳二元酸之用途 2 1.2.1 合成香料 2 1.2.2 合成尼龍材料 2 1.2.3 合成潤滑油 2 1.3 生物性氧化 3 1.3.1 生物性氧化機制 3 1.3.2 氧化過程之運輸機制 4 1.3.2.1 運輸進入菌體 4 1.3.2.2 運輸於菌體內各胞器 5 1.4 微生物醱酵生產長鏈二元酸之策略 6 1.4.1 以基因工程技術改良菌株 6 1.4.1.1 破壞脂肪酸降解之酵素 6 1.4.1.3 強化表現正烷類末端氧化之酵素 7 1.4.2 以致突變改良菌株 7 1.4.3 產程設計 7 1.4.4 微生物醱酵生產長鏈二元酸之產業現況 8 1.5 醱酵策略簡介 9 1.6 研究目的 10 貳、材料與方法 11 2.1 菌株來源 11 2.2 Hinton 氏三角搖瓶培養 11 2.2.1 Candida tropicalis ATCC 20962 生長曲線 11 2.2.2搖瓶培養 11 2.3 醱酵槽培養 12 2.3.1 批式培養 12 2.3.2 批式饋料培養 12 2.4 菌株突變 13 2.4.1 紫外線致突變 13 2.4.2 突變株篩選 14 2.5 分析方法 14 2.5.1 菌體乾重 14 2.5.2 比生長速率 14 2.5.3 生菌數 15 2.5.4 葡萄糖濃度 15 2.5.5 十二碳二元酸濃度分析 83 15 2.5.5.1 萃取醱酵液中十二碳二元酸 15 2.5.5.2 十二碳二元酸之氣相層析分析 15 2.5.6 十二碳二元酸之初步回收 16 參、結果與討論 17 3-1 Hinton 氏三角搖瓶培養 17 3-1-1 Candida tropicalis ATCC 20962 生長曲線 17 3-1-2 搖瓶培養 17 3-2 醱酵槽培養 18 3-2-1 批式培養 18 3-2-2 批式饋料培養 18 3-2-2-1 饋料培養基之組成 19 3-2-2-2 十二碳二元酸轉化效率與微量元素及維生素之添加 21 3-2-3 十二碳二元酸之初步回收 23 3-3 熱帶假絲酵母菌株突變之篩選 23 3-3-1 紫外線之致死曲線 23 3-3-2 突變株之篩選 24 肆、結論 25 伍、未來展望 25 圖表 26 參考文獻 49 附錄 57 | |
dc.language.iso | zh-TW | |
dc.title | Candida tropicalis ATCC 20962 之十二碳二元酸轉化生產研究 | zh_TW |
dc.title | Bioconversion of 1,12-dodecanedioic acid by Candida tropicalis ATCC 20962 | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇遠志,黃健雄,劉?德 | |
dc.subject.keyword | 熱帶假絲酵母,十二碳二元酸,批式饋料培養,紫外線致突變, | zh_TW |
dc.subject.keyword | Candida tropicalis,dodecanedioic acid (DCA12),fed-batch culture,mutagenesis by UV irradiation, | en |
dc.relation.page | 58 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2013-08-14 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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