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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17131完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭彥彬 | |
| dc.contributor.author | Chun-Hao Chen | en |
| dc.contributor.author | 陳君豪 | zh_TW |
| dc.date.accessioned | 2021-06-07T23:57:40Z | - |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-17 | |
| dc.identifier.citation | Abreu, J. G., Ketpura, N. I., Reversade, B. & De Robertis, E. M. (2002) Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta. Nat Cell Biol 4, 599-604. doi:10.1038/ncb826.
Alvarez, H., Corvalan, A., Roa, J. C., Argani, P., Murillo, F., Edwards, J., Beaty, R., Feldmann, G., Hong, S. M., Mullendore, M., Roa, I., Ibanez, L., Pimentel, F., Diaz, A., Riggins, G. J. & Maitra, A. (2008) Serial analysis of gene expression identifies connective tissue growth factor expression as a prognostic biomarker in gallbladder cancer. Clin Cancer Res 14, 2631-2638. doi:10.1158/1078-0432.CCR-07-1991. Atilla, G. & Kutukculer, N. (1998) Crevicular fluid interleukin-1beta, tumor necrosis factor-alpha, and interleukin-6 levels in renal transplant patients receiving cyclosporine A. J Periodontol 69, 784-790. doi:10.1902/jop.1998.69.7.784. Ayanoglou, C. M. & Lesty, C. (1999) Cyclosporin A-induced gingival overgrowth in the rat: a histological, ultrastructural and histomorphometric evaluation. J Periodontal Res 34, 7-15. Babic, A. M., Chen, C. C. & Lau, L. F. (1999) Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo. Mol Cell Biol 19, 2958-2966. Ball, D. K., Rachfal, A. W., Kemper, S. A. & Brigstock, D. R. (2003) The heparin-binding 10 kDa fragment of connective tissue growth factor (CTGF) containing module 4 alone stimulates cell adhesion. J Endocrinol 176, R1-7. Black, S. A., Jr., Palamakumbura, A. H., Stan, M. & Trackman, P. C. (2007) Tissue-specific mechanisms for CCN2/CTGF persistence in fibrotic gingiva: interactions between cAMP and MAPK signaling pathways, and prostaglandin E2-EP3 receptor mediated activation of the c-JUN N-terminal kinase. J Biol Chem 282, 15416-15429. doi:10.1074/jbc.M610432200. Black, S. A., Jr. & Trackman, P. C. (2008) Transforming growth factor-beta1 (TGFbeta1) stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibroblasts through a RhoA-independent, Rac1/Cdc42-dependent mechanism: statins with forskolin block TGFbeta1-induced CCN2/CTGF expression. J Biol Chem 283, 10835-10847. doi:10.1074/jbc.M710363200. Bolzani, G., Della Coletta, R., Martelli Junior, H. & Graner, E. (2000) Cyclosporin A inhibits production and activity of matrix metalloproteinases by gingival fibroblasts. J Periodontal Res 35, 51-58. Bonnaure-Mallet, M., Tricot-Doleux, S. & Godeau, G. J. (1995) Changes in extracellular matrix macromolecules in human gingiva after treatment with drugs inducing gingival overgrowth. Arch Oral Biol 40, 393-400. Bradham, D. M., Igarashi, A., Potter, R. L. & Grotendorst, G. R. (1991) Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10. J Cell Biol 114, 1285-1294. Brigstock, D. R., Steffen, C. L., Kim, G. Y., Vegunta, R. K., Diehl, J. R. & Harding, P. A. (1997) Purification and characterization of novel heparin-binding growth factors in uterine secretory fluids. Identification as heparin-regulated Mr 10,000 forms of connective tissue growth factor. J Biol Chem 272, 20275-20282. Buduneli, N., Kutukculer, N., Aksu, G. & Atilla, G. (2001) Evaluation of transforming growth factor-beta 1 level in crevicular fluid of cyclosporin A-treated patients. J Periodontol 72, 526-531. doi:10.1902/jop.2001.72.4.526. Burgess, J. K. (2005) Connective tissue growth factor: a role in airway remodelling in asthma? Clin Exp Pharmacol Physiol 32, 988-994. doi:10.1111/j.1440-1681.2005.04296.x. Burgess, J. K., Ge, Q., Poniris, M. H., Boustany, S., Twigg, S. M., Black, J. L. & Johnson, P. R. (2006) Connective tissue growth factor and vascular endothelial growth factor from airway smooth muscle interact with the extracellular matrix. Am J Physiol Lung Cell Mol Physiol 290, L153-161. doi:10.1152/ajplung.00287.2005. Chang, J. Z., Yang, W. H., Deng, Y. T., Chen, H. M. & Kuo, M. Y. (2012) Thrombin-stimulated connective tissue growth factor (CTGF/CCN2) production in human buccal mucosal fibroblasts: Inhibition by epigallocatechin-3-gallate. Head Neck 34, 1089-1094. doi:10.1002/hed.21863. Chang, J. Z., Yang, W. H., Deng, Y. T., Chen, H. M. & Kuo, M. Y. (2013) EGCG blocks TGFbeta1-induced CCN2 by suppressing JNK and p38 in buccal fibroblasts. Clin Oral Investig 17, 455-461. doi:10.1007/s00784-012-0713-5. Chen, C. C., Chen, N. & Lau, L. F. (2001) The angiogenic factors Cyr61 and connective tissue growth factor induce adhesive signaling in primary human skin fibroblasts. J Biol Chem 276, 10443-10452. doi:10.1074/jbc.M008087200. Chen, Y. T., Tu, H. P., Chin, Y. T., Shen, E. C., Chiang, C. Y., Gau, C. H. & Fu, E. (2005) Upregulation of transforming growth factor-beta1 and vascular endothelial growth factor gene and protein expression in cyclosporin-induced overgrown edentulous gingiva in rats. J Periodontol 76, 2267-2275. doi:10.1902/jop.2005.76.12.2267. Chen, Y. W., Yang, W. H., Wong, M. Y., Chang, H. H. & Yen-Ping Kuo, M. (2012) Curcumin inhibits thrombin-stimulated connective tissue growth factor (CTGF/CCN2) production through c-Jun NH2-terminal kinase suppression in human gingival fibroblasts. J Periodontol 83, 1546-1553. doi:10.1902/jop.2012.110641. Chiang, C. Y., Chen, Y. T., Hung, F. M., Tu, H. P., Fu, M. M. & Fu, E. (2007) Cyclosporin-A inhibits the expression of cyclooxygenase-2 in gingiva. J Periodontal Res 42, 443-449. doi:10.1111/j.1600-0765.2006.00967.x. Chiang, C. Y., Tu, H. P., Chen, Y. T., Chin, Y. T., Lai, T. M., Chiu, H. C., Nieh, S. & Fu, E. (2011) Up-regulation of retinoblastoma protein phosphorylation in gingiva after cyclosporine A treatment: an in vivo and in vitro study. J Periodontal Res 46, 158-163. doi:10.1111/j.1600-0765.2010.01312.x. Chin, Y. T., Chen, Y. T., Tu, H. P., Shen, E. C., Chiang, C. Y., Gau, C. H., Nieh, S. & Fu, E. (2006) Upregulation of the expression of epidermal growth factor and its receptor in gingiva upon cyclosporin A treatment. J Periodontol 77, 647-656. doi:10.1902/jop.2006.050130. Chin, Y. T., Liao, Y. W., Fu, M. M., Tu, H. P., Shen, E. C., Nieh, S., Shih, K. C. & Fu, E. (2011) Nrf-2 regulates cyclosporine-stimulated HO-1 expression in gingiva. J Dent Res 90, 995-1000. doi:10.1177/0022034511410698. Chin, Y. T., Tu, H. P., Chen, Y. T., Dai, N. T., Shen, E. C., Chiang, C. Y., Fu, M. M. & Fu, E. (2009) Expression and bioactivities of endothelin-1 in gingiva during cyclosporine A treatment. J Periodontal Res 44, 35-42. doi:10.1111/j.1600-0765.2007.01058.x. Chiu, H. C., Lan, G. L., Chiang, C. Y., Chin, Y. T., Tu, H. P., Ming-Jen Fu, M., Shin, N. & Fu, E. (2008) Upregulation of heme oxygenase-1 expression in gingiva after cyclosporin A treatment. J Periodontol 79, 2200-2206. doi:10.1902/jop.2008.080160. Chiu, H. C., Lu, Y. T., Chin, Y. T., Tu, H. P., Chiang, C. Y., Gau, C. H., Nieh, S. & Fu, E. (2009) Cyclosporine A inhibits the expression of membrane type-I matrix metalloproteinase in gingiva. J Periodontal Res 44, 338-347. doi:10.1111/j.1600-0765.2008.01126.x. Das, S. J., Parkar, M. H. & Olsen, I. (2001) Upregulation of keratinocyte growth factor in cyclosporin A-induced gingival overgrowth. J Periodontol 72, 745-752. doi:10.1902/jop.2001.72.6.745. di Mola, F. F., Friess, H., Martignoni, M. E., Di Sebastiano, P., Zimmermann, A., Innocenti, P., Graber, H., Gold, L. I., Korc, M. & Buchler, M. W. (1999) Connective tissue growth factor is a regulator for fibrosis in human chronic pancreatitis. Ann Surg 230, 63-71. Frazier, K., Williams, S., Kothapalli, D., Klapper, H. & Grotendorst, G. R. (1996) Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor. J Invest Dermatol 107, 404-411. Gao, R. & Brigstock, D. R. (2003) Low density lipoprotein receptor-related protein (LRP) is a heparin-dependent adhesion receptor for connective tissue growth factor (CTGF) in rat activated hepatic stellate cells. Hepatol Res 27, 214-220. Grotendorst, G. R. (1997) Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts. Cytokine Growth Factor Rev 8, 171-179. Hall, E. E. (1997) Prevention and treatment considerations in patients with drug-induced gingival enlargement. Curr Opin Periodontol 4, 59-63. Heering, P. & Grabensee, B. (1991) Influence of ciclosporin A on renal tubular function after kidney transplantation. Nephron 59, 66-70. Heng, E. C., Huang, Y., Black, S. A., Jr. & Trackman, P. C. (2006) CCN2, connective tissue growth factor, stimulates collagen deposition by gingival fibroblasts via module 3 and alpha6- and beta1 integrins. J Cell Biochem 98, 409-420. doi:10.1002/jcb.20810. Holmes, A., Abraham, D. J., Chen, Y., Denton, C., Shi-wen, X., Black, C. M. & Leask, A. (2003) Constitutive connective tissue growth factor expression in scleroderma fibroblasts is dependent on Sp1. J Biol Chem 278, 41728-41733. doi:10.1074/jbc.M305019200. Holmes, A., Abraham, D. J., Sa, S., Shiwen, X., Black, C. M. & Leask, A. (2001) CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signaling. J Biol Chem 276, 10594-10601. doi:10.1074/jbc.M010149200. Hyland, P. L., McKeown, S. T., Mackenzie, I. C. & Irwin, C. R. (2004) Regulation of keratinocyte growth factor and scatter factor in cyclosporin-induced gingival overgrowth. J Oral Pathol Med 33, 391-397. doi:10.1111/j.1600-0714.2004.00223.x. Iacopino, A. M., Doxey, D., Cutler, C. W., Nares, S., Stoever, K., Fojt, J., Gonzales, A. & Dill, R. E. (1997) Phenytoin and cyclosporine A specifically regulate macrophage phenotype and expression of platelet-derived growth factor and interleukin-1 in vitro and in vivo: possible molecular mechanism of drug-induced gingival hyperplasia. J Periodontol 68, 73-83. doi:10.1902/jop.1997.68.1.73. Igarashi, A., Nashiro, K., Kikuchi, K., Sato, S., Ihn, H., Grotendorst, G. R. & Takehara, K. (1995) Significant correlation between connective tissue growth factor gene expression and skin sclerosis in tissue sections from patients with systemic sclerosis. J Invest Dermatol 105, 280-284. Ikawa, Y., Ng, P. S., Endo, K., Kondo, M., Chujo, S., Ishida, W., Shirasaki, F., Fujimoto, M. & Takehara, K. (2008) Neutralizing monoclonal antibody to human connective tissue growth factor ameliorates transforming growth factor-beta-induced mouse fibrosis. J Cell Physiol 216, 680-687. doi:10.1002/jcp.21449. Ito, H., Hirata, Y., Adachi, S., Tanaka, M., Tsujino, M., Koike, A., Nogami, A., Murumo, F. & Hiroe, M. (1993) Endothelin-1 is an autocrine/paracrine factor in the mechanism of angiotensin II-induced hypertrophy in cultured rat cardiomyocytes. J Clin Invest 92, 398-403. doi:10.1172/JCI116579. Ito, Y., Aten, J., Bende, R. J., Oemar, B. S., Rabelink, T. J., Weening, J. J. & Goldschmeding, R. (1998) Expression of connective tissue growth factor in human renal fibrosis. Kidney Int 53, 853-861. doi:10.1111/j.1523-1755.1998.00820.x. Jedsadayanmata, A., Chen, C. C., Kireeva, M. L., Lau, L. F. & Lam, S. C. (1999) Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3). J Biol Chem 274, 24321-24327. Kahan, B. D. (1989) Cyclosporine. N Engl J Med 321, 1725-1738. doi:10.1056/NEJM198912213212507. Kantarci, A., Black, S. A., Xydas, C. E., Murawel, P., Uchida, Y., Yucekal-Tuncer, B., Atilla, G., Emingil, G., Uzel, M. I., Lee, A., Firatli, E., Sheff, M., Hasturk, H., Van Dyke, T. E. & Trackman, P. C. (2006) Epithelial and connective tissue cell CTGF/CCN2 expression in gingival fibrosis. J Pathol 210, 59-66. doi:10.1002/path.2000. Kawaki, H., Kubota, S., Suzuki, A., Suzuki, M., Kohsaka, K., Hoshi, K., Fujii, T., Lazar, N., Ohgawara, T., Maeda, T., Perbal, B., Takano-Yamamoto, T. & Takigawa, M. (2011) Differential roles of CCN family proteins during osteoblast differentiation: Involvement of Smad and MAPK signaling pathways. Bone 49, 975-989. doi:10.1016/j.bone.2011.06.033. Kucich, U., Rosenbloom, J. C., Herrick, D. J., Abrams, W. R., Hamilton, A. D., Sebti, S. M. & Rosenbloom, J. (2001) Signaling events required for transforming growth factor-beta stimulation of connective tissue growth factor expression by cultured human lung fibroblasts. Arch Biochem Biophys 395, 103-112. doi:10.1006/abbi.2001.2571. Lasky, J. A., Ortiz, L. A., Tonthat, B., Hoyle, G. W., Corti, M., Athas, G., Lungarella, G., Brody, A. & Friedman, M. (1998) Connective tissue growth factor mRNA expression is upregulated in bleomycin-induced lung fibrosis. Am J Physiol 275, L365-371. Leask, A. & Abraham, D. J. (2003) The role of connective tissue growth factor, a multifunctional matricellular protein, in fibroblast biology. Biochem Cell Biol 81, 355-363. doi:10.1139/o03-069. Leask, A., Holmes, A., Black, C. M. & Abraham, D. J. (2003) Connective tissue growth factor gene regulation. Requirements for its induction by transforming growth factor-beta 2 in fibroblasts. J Biol Chem 278, 13008-13015. doi:10.1074/jbc.M210366200. Luft, F. C. (2008) CCN2, the connective tissue growth factor. J Mol Med (Berl) 86, 1-3. doi:10.1007/s00109-007-0287-x. Mandel, S. A., Amit, T., Weinreb, O., Reznichenko, L. & Youdim, M. B. (2008) Simultaneous manipulation of multiple brain targets by green tea catechins: a potential neuroprotective strategy for Alzheimer and Parkinson diseases. CNS Neurosci Ther 14, 352-365. doi:10.1111/j.1755-5949.2008.00060.x. McLennan, S. V., Wang, X. Y., Moreno, V., Yue, D. K. & Twigg, S. M. (2004) Connective tissue growth factor mediates high glucose effects on matrix degradation through tissue inhibitor of matrix metalloproteinase type 1: implications for diabetic nephropathy. Endocrinology 145, 5646-5655. doi:10.1210/en.2004-0436. Meng, M., Li, Y. Q., Yan, M. X., Kou, Y. & Ren, H. B. (2007) Effects of epigallocatechin gallate on diethyldithiocarbamate-induced pancreatic fibrosis in rats. Biol Pharm Bull 30, 1091-1096. Mori, T., Kawara, S., Shinozaki, M., Hayashi, N., Kakinuma, T., Igarashi, A., Takigawa, M., Nakanishi, T. & Takehara, K. (1999) Role and interaction of connective tissue growth factor with transforming growth factor-beta in persistent fibrosis: A mouse fibrosis model. J Cell Physiol 181, 153-159. doi:10.1002/(SICI)1097-4652(199910)181:1<153::AID-JCP16>3.0.CO;2-K. Myrillas, T. T., Linden, G. J., Marley, J. J. & Irwin, C. R. (1999) Cyclosporin A regulates interleukin-1beta and interleukin-6 expression in gingiva: implications for gingival overgrowth. J Periodontol 70, 294-300. doi:10.1902/jop.1999.70.3.294. Naesens, M., Kuypers, D. R. & Sarwal, M. (2009) Calcineurin inhibitor nephrotoxicity. Clin J Am Soc Nephrol 4, 481-508. doi:10.2215/CJN.04800908. Nakachi, K., Suemasu, K., Suga, K., Takeo, T., Imai, K. & Higashi, Y. (1998) Influence of drinking green tea on breast cancer malignancy among Japanese patients. Jpn J Cancer Res 89, 254-261. Nares, S., Ng, M. C., Dill, R. E., Park, B., Cutler, C. W. & Iacopino, A. M. (1996) Cyclosporine A upregulates platelet-derived growth factor B chain in hyperplastic human gingiva. J Periodontol 67, 271-278. doi:10.1902/jop.1996.67.3.271. Paradis, V., Dargere, D., Vidaud, M., De Gouville, A. C., Huet, S., Martinez, V., Gauthier, J. M., Ba, N., Sobesky, R., Ratziu, V. & Bedossa, P. (1999) Expression of connective tissue growth factor in experimental rat and human liver fibrosis. Hepatology 30, 968-976. doi:10.1002/hep.510300425. Plemons, J. M., Dill, R. E., Rees, T. D., Dyer, B. J., Ng, M. C. & Iacopino, A. M. (1996) PDGF-B producing cells and PDGF-B gene expression in normal gingival and cyclosporine A-induced gingival overgrowth. J Periodontol 67, 264-270. doi:10.1902/jop.1996.67.3.264. Sander, B., Brigati, C. & Moller, E. (1986) Inhibition of in vitro alloreactivity by cyclosporin A: evidence for an inter-individual variation in sensitivity. Scand J Immunol 23, 435-440. Seymour, R. A. & Jacobs, D. J. (1992) Cyclosporin and the gingival tissues. J Clin Periodontol 19, 1-11. Sheng, R., Gu, Z. L., Xie, M. L., Zhou, W. X. & Guo, C. Y. (2009) EGCG inhibits proliferation of cardiac fibroblasts in rats with cardiac hypertrophy. Planta Med 75, 113-120. doi:10.1055/s-0028-1088387. Shi-Wen, X., Denton, C. P., Dashwood, M. R., Holmes, A. M., Bou-Gharios, G., Pearson, J. D., Black, C. M. & Abraham, D. J. (2001) Fibroblast matrix gene expression and connective tissue remodeling: role of endothelin-1. J Invest Dermatol 116, 417-425. doi:10.1046/j.1523-1747.2001.01256.x. Silva, H. C., Coletta, R. D., Jorge, J., Bolzani, G., de Almeida, O. P. & Graner, E. (2001) The effect of cyclosporin A on the activity of matrix metalloproteinases during the healing of rat molar extraction wounds. Arch Oral Biol 46, 875-879. Sriram, N., Kalayarasan, S. & Sudhandiran, G. (2009) Epigallocatechin-3-gallate exhibits anti-fibrotic effect by attenuating bleomycin-induced glycoconjugates, lysosomal hydrolases and ultrastructural changes in rat model pulmonary fibrosis. Chem Biol Interact 180, 271-280. doi:10.1016/j.cbi.2009.02.017. Stratton, R., Rajkumar, V., Ponticos, M., Nichols, B., Shiwen, X., Black, C. M., Abraham, D. J. & Leask, A. (2002) Prostacyclin derivatives prevent the fibrotic response to TGF-beta by inhibiting the Ras/MEK/ERK pathway. FASEB J 16, 1949-1951. doi:10.1096/fj.02-0204fje. Tipoe, G. L., Leung, T. M., Liong, E. C., Lau, T. Y., Fung, M. L. & Nanji, A. A. (2010) Epigallocatechin-3-gallate (EGCG) reduces liver inflammation, oxidative stress and fibrosis in carbon tetrachloride (CCl4)-induced liver injury in mice. Toxicology 273, 45-52. doi:10.1016/j.tox.2010.04.014. Tipton, D. A., Stricklin, G. P. & Dabbous, M. K. (1991) Fibroblast heterogeneity in collagenolytic response to cyclosporine. J Cell Biochem 46, 152-165. doi:10.1002/jcb.240460209. Trackman, P. C. & Kantarci, A. (2004) Connective tissue metabolism and gingival overgrowth. Crit Rev Oral Biol Med 15, 165-175. Uzel, M. I., Kantarci, A., Hong, H. H., Uygur, C., Sheff, M. C., Firatli, E. & Trackman, P. C. (2001) Connective tissue growth factor in drug-induced gingival overgrowth. J Periodontol 72, 921-931. doi:10.1902/jop.2001.72.7.921. Van Beek, J. P., Kennedy, L., Rockel, J. S., Bernier, S. M. & Leask, A. (2006) The induction of CCN2 by TGFbeta1 involves Ets-1. Arthritis Res Ther 8, R36. doi:10.1186/ar1890. van der Stroom, J. G., van Wezel, H. B., Piek, J. J., Kal, J. E., van der Linden, R., Vergroesen, I., Pfaffendorf, M. & van Zwieten, P. A. (1999) Intracoronary-administered urapidil does not influence myocardial contractility, metabolic activity, or coronary sinus blood flow in humans. J Cardiothorac Vasc Anesth 13, 684-689. Vinson, J. A., Teufel, K. & Wu, N. (2004) Green and black teas inhibit atherosclerosis by lipid, antioxidant, and fibrinolytic mechanisms. J Agric Food Chem 52, 3661-3665. doi:10.1021/jf035255l. Williamson, M. S., Miller, E. K., Plemons, J., Rees, T. & Iacopino, A. M. (1994) Cyclosporine A upregulates interleukin-6 gene expression in human gingiva: possible mechanism for gingival overgrowth. J Periodontol 65, 895-903. doi:10.1902/jop.1994.65.10.895. Xu, S., Denton, C. P., Holmes, A., Dashwood, M. R., Abraham, D. J. & Black, C. M. (1998) Endothelins: effect on matrix biosynthesis and proliferation in normal and scleroderma fibroblasts. J Cardiovasc Pharmacol 31 Suppl 1, S360-363. Yang, W. H., Kuo, M. Y., Liu, C. M., Deng, Y. T., Chang, H. H. & Chang, J. Z. (2013) Curcumin Inhibits TGFbeta1-induced CCN2 via Src, JNK, and Smad3 in Gingiva. J Dent Res 92, 629-634. doi:10.1177/0022034513488139. Young, B. A., Burdmann, E. A., Johnson, R. J., Alpers, C. E., Giachelli, C. M., Eng, E., Andoh, T., Bennett, W. M. & Couser, W. G. (1995) Cellular proliferation and macrophage influx precede interstitial fibrosis in cyclosporine nephrotoxicity. Kidney Int 48, 439-448. Yumei, F., Zhou, Y., Zheng, S. & Chen, A. (2006) The antifibrogenic effect of (-)-epigallocatechin gallate results from the induction of de novo synthesis of glutathione in passaged rat hepatic stellate cells. Lab Invest 86, 697-709. doi:10.1038/labinvest.3700425. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17131 | - |
| dc.description.abstract | 牙齦增生是指牙齦上皮組織或結締組織的細胞不正常的過度增殖，使牙齦組織呈現擴大的狀態，根據組織學的特徵可分為炎性增生、纖維性增生或兩者合併。結締組織生長因子(connective tissue growth factor, CTGF/CCN2)是一種間質蛋白，屬於CCN家族的一員，在牙齦增生的組織中常可偵測到結締組織生長因子的上升，且其表現量與牙齦纖維化增生的程度呈正相關。環孢素(cyclosporin A, CsA)是一種免疫抑制劑，這種藥物與抗癲癇藥物phenytoin以及鈣離子阻斷劑常會造成牙齦增生的副作用。我們利用初級培養正常的牙齦纖維母細胞，以反轉錄聚合酶連鎖反應以及西方墨點法觀察環孢素是否能誘發結締組織生長因子的表現。並加入各種天然化學預防藥劑與抑制劑，探討植物生化素對環孢素誘發結締組織生長因子表現的影響。結果發現，環孢素會誘導人類牙齦纖維母細胞表現結締組織生長因子，經環孢素刺激的人類牙齦纖維母細胞，其結締組織生長因子的表現會在2小時達到高峰。JNK和Smad3的抑制劑(SP600125, SIS3)也會抑制環孢素誘導人類牙齦纖維母細胞表現結締組織生長因子。兒茶素(epigallocatechin gallate, EGCG)會抑制環孢素誘導人類牙齦纖維母細胞表現結締組織生長因子，且抑制效果具有濃度依賴性。未來仍需進一步的實驗來確認兒茶素在環孢素引起的牙齦增生中的抑制角色。 | zh_TW |
| dc.description.abstract | Gingival overgrowth is clinically manifested by increased gingival volume, including an increased number of cells and a higher level of extracellular matrix production. Histologically, it can be inflammatory and/or fibrous. Connective tissue growth factor(CTGF/CCN2) is a matricellular protein of the CCN protein family. It is overexpressed in both drug-induced and non-drug-induced gingival overgrowth tissues and is positively related to the degree of fibrosis. Cyclosporin A(CsA) is a selective immunosuppressant that has a variety of applications in medical practice. Like phenytoin and calcium channel blockers, the drug is associated with gingival overgrowth. In this study, human gingival fibroblasts(HGFs) were obtained from normal gingiva and were primary cultured in vitro. CsA-induced CTGF expression were assessed by quantitative reverse transcription polymerase chain reaction and Western blot analysis. Effects of natural chemopreventive drugs and inhibitors of various signaling pathways on CsA-induced CTGF expression were also investigated The results showed that CsA increased CTGF synthesis and that CTGF expression reached the maximal level after 2 hours exposure to CsA. Pretreatment with c-Jun NH2-terminal kinase(JNK) inhibitor SP600125 and SIS3 significantly reduced CsA-induced CTGF expression in HGFs. In addition, EGCG dose-dependently inhibited CsA-induced CTGF expression in HGFs. Additional research is required to confirm the inhibiting role of EGCG in the development of gingival overgrowth. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T23:57:40Z (GMT). No. of bitstreams: 1 ntu-102-R99422021-1.pdf: 1133236 bytes, checksum: 542dfcbadf1259f5bcb3720374dac96f (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 目錄
誌謝 I 中文摘要 II Abstract III 目錄 IV 圖目錄 VI 第一章 緒論 1 一、 牙齦構造 1 二、 牙齦增生 3 I. 牙齦之炎性增生 3 II. 牙齦之纖維性增生 4 III. 藥物與牙齦增生 5 三、 環孢素(cyclosporin A, CsA) 6 四、 結締組織生長因子(connective tissue growth factor, CTGF) 9 五、 兒茶素(epigallocatechin gallate, EGCG) 12 第二章 研究目的 13 第三章 實驗材料與方法 14 一、 細胞株與細胞培養(cell culture) 14 二、 藥物處理 14 I. CsA的處理 15 II. Inhibitor使用資料 15 三、 反轉錄聚合酶連鎖反應(Reverse Transcription Polymerase Chain Reaction；RT-PCR) 15 I. RNA萃取 16 II. RNA定量 16 III. 反轉錄(Reverse Transcription；RT) 16 IV. 聚合酵素連鎖反應(Polymerase Chain Reaction；PCR) 17 V. 瓊脂凝膠電泳分析(Agarose gel electrophoresis) 17 四、 定量聚合酶連鎖反應 (Real-time Polymerase Chain Reaction；Real-time PCR；qPCR) 17 五、 西方墨點法 18 I. 蛋白質萃取 18 II. 蛋白質濃度測定 18 III. 膠體配置與電泳分析 19 IV. 蛋白質轉漬 19 V. 抗體的使用與顯影呈色 19 VI. 統計分析 20 第四章 結果 21 CsA誘導人類牙齦纖維母細胞中CTGF蛋白於不同時間點的表現 21 CsA誘導人類牙齦纖維母細胞中CTGF蛋白於不同濃度的表現 21 CsA誘導人類牙齦纖維母細胞中CTGF mRNA於不同時間點的表現 21 JNK和Smad3抑制劑會抑制CsA對於CTGF的誘導 22 EGCG可以抑制CsA誘導人類牙齦纖維母細胞之CTGF蛋白的表現 22 不同濃度EGCG對CsA誘發之CTGF protein level的抑制效果 23 第五章 討論 24 第六章 結論 26 參考文獻 34 圖目錄 圖 一A、不同時間點CsA誘導HGF表現CTGF蛋白的情形 27 圖 一B、不同時間點CsA誘導HGF表現CTGF蛋白的定量 27 圖 二A、不同濃度之CsA誘導HGF表現CTGF蛋白的情形 28 圖 二B、不同濃度之CsA誘導HGF表現CTGF蛋白的定量 28 圖 三、不同時間點CsA誘導HGF表現CTGF mRNA的情形 29 圖 四A、不同抑制劑前處理對CsA誘導HGF表現CTGF蛋白的影響-1 30 圖 四B、不同抑制劑前處理影響CsA誘導HGF表現CTGF蛋白的定量-1 30 圖 五A、不同抑制劑前處理對CsA誘導HGF表現CTGF蛋白的影響-2 31 圖 五B、不同抑制劑前處理影響CsA誘導HGF表現CTGF蛋白的定量-2 31 圖 六A、不同天然化學預防藥劑抑制CsA誘導HGF表現CTGF蛋白的情形 32 圖 六B、不同天然化學預防藥劑抑制CsA誘導HGF表現CTGF蛋白的定量 32 圖 七A、不同濃度EGCG抑制CsA誘導HGF表現CTGF蛋白的情形 33 圖 七B、不同濃度EGCG抑制CsA誘導HGF表現CTGF蛋白的定量 33 | |
| dc.language.iso | zh-TW | |
| dc.subject | 兒茶素 | zh_TW |
| dc.subject | 環孢素 | zh_TW |
| dc.subject | 牙齦增生 | zh_TW |
| dc.subject | 結締組織生長因子 | zh_TW |
| dc.subject | cyclosporin A | en |
| dc.subject | EGCG | en |
| dc.subject | connective tissue growth factor | en |
| dc.subject | gingival overgrowth | en |
| dc.title | 環孢素誘導牙齦纖維母細胞表現結締組織生長因子機制之研究 | zh_TW |
| dc.title | Cyclosporin A Induced Connective Tissue Growth Factor in Human Gingival Fibroblasts | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張瑞青,周涵怡 | |
| dc.subject.keyword | 環孢素,牙齦增生,結締組織生長因子,兒茶素, | zh_TW |
| dc.subject.keyword | cyclosporin A,gingival overgrowth,connective tissue growth factor,EGCG, | en |
| dc.relation.page | 41 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-08-18 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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